Title:
SCREENING SYSTEM FOR SUBSTANCE INHIBITING INFLAMMATORY CHANGE OF ADIPOCYTE AND SCREENING METHOD USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2006/112418
Kind Code:
A1
Abstract:
It is intended to provide a screening system for screening a substance effectively inhibiting an inflammatory change of adipocyte and a screening method using the same. The screening system is a screening system for an inhibitor that inhibits a paracrine factor or an intracellular signal related to an increase in the inflammatory change of adipocyte comprising coculture of an adipocyte and macrophage. The screening method is a screening method for an inhibitor that inhibits a paracrine factor or an intracellular signal characterized by adding a test substance to the screening system and measuring a change of gene expression level of paracrine factor or inflammatory cytokine by the test substance.
Inventors:
OGAWA YOSHIHIRO
SUGANAMI TAKAYOSHI
SUGANAMI TAKAYOSHI
Application Number:
PCT/JP2006/307983
Publication Date:
October 26, 2006
Filing Date:
April 14, 2006
Export Citation:
Assignee:
UNIV TOKYO MEDICAL & DENTAL (JP)
OGAWA YOSHIHIRO
SUGANAMI TAKAYOSHI
OGAWA YOSHIHIRO
SUGANAMI TAKAYOSHI
International Classes:
C12Q1/02; C12N5/077; C12N15/09; G01N33/15; G01N33/50
Other References:
SUGANAMI T. ET AL.: "A paracrine loop between adipocytes ad macrophages aggravates inflammatory changes", ARTERIOSCLER. THROMB. VASC. BIOL., vol. 25, no. 10, October 2005 (2005-10-01), pages 2062 - 2068, XP003006850
OGAWA Y.: "Shibo Saibo to Macrophage no Sogo Sayo", IGAKU NO AYUMI, vol. 217, no. 1, 1 April 2006 (2006-04-01), pages 147 - 152, XP003006851
SUGANAMI T. ET AL.: "Metabolic Syndrome no Bunshi Kiban: Shibo Saibo to Macrophage no Paracrine Chosetsu", FOLIA ENDOCRINOLOGICA JAPONICA, THE JAPAN ENDOCRINE SOCIETY BUNKAKAI SHOROKUSHU, vol. 81, no. 2, 2005, pages 541, XP003006852
SUGANAMI T. ET AL.: "Himan no Shibo Soshiki ni Okeru Shibo Saibo to Macrophage no paracrine Chosetsukei - Kyobaiyokei o Mochiita Kento -", DAI 26 KAI JAPAN SOCIETY FOR THE STUDY OF OBESITY PROGRAM SHOROKUSHU, vol. 11, no. SUPPLEMENT, 2005, pages 117, XP003006853
TOYODA T. ET AL.: "Shibo Saibo to Macrophage no Sogo Sayo ni Okeru Kakunai Juyotai Agonist no Koensho Sayo", JOURNAL OF JAPAN SOCIETY FOR THE STUDY OF OBESITY 'HIMAN KENKYU', DAI 26 KAI JAPAN SOCIETY FOR THE STUDY OF OBESITY PROGRAM SHOROKUSHU, vol. 11, no. SUPPLEMENT, 2005, pages 172, XP003006854
SUGANAMI T. ET AL.: "Kyobaiyokei o Mochiita Shibo Saibo. Macrophage Sogo Sayo no Bunshi Kiko no Kento", THE JOURNAL OF THE JAPAN DIABETIC SOCIETY, vol. 48, no. SUPPLEMENT 2, 25 April 2005 (2005-04-25), pages S-141, XP003006855
NISHIDA J. ET AL.: "Shibo Saibo. Macrophage Kyobaiyokei ni Okeru Shibo Saibo Enshosei Henka no Bunshi Kiko no Kento", FOLIA ENDOCRINOLOGICA JAPONICA, DAI 78 KAI THE JAPAN ENDOCRINE SOCIETY GAKUJUTSU SOKAI SHOROKUSHU, vol. 81, no. 1, 20 April 2005 (2005-04-20), pages 103, XP003006856
HOTAMISLIGIL G.S. ET AL.: "Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance", SCIENCE, vol. 259, 1993, pages 87 - 91, XP002973111
CLEMENT K. ET AL.: "Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects", FASEB J., vol. 18, 2004, pages 1657 - 1669, XP003006857
WEISBERG S.P. ET AL.: "Obesity is associated with macrophage accumulation in adipose tissue", J. CLIN. INVEST., vol. 112, no. 12, 2003, pages 1796 - 1808, XP003006858
XU H. ET AL.: "Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance", J. CLIN. INVEST., vol. 112, no. 12, 2003, pages 1821 - 1830, XP003006859
OGAWA Y.: "Shibo Saibo to Macrophage no Sogo Sayo", IGAKU NO AYUMI, vol. 217, no. 1, 1 April 2006 (2006-04-01), pages 147 - 152, XP003006851
SUGANAMI T. ET AL.: "Metabolic Syndrome no Bunshi Kiban: Shibo Saibo to Macrophage no Paracrine Chosetsu", FOLIA ENDOCRINOLOGICA JAPONICA, THE JAPAN ENDOCRINE SOCIETY BUNKAKAI SHOROKUSHU, vol. 81, no. 2, 2005, pages 541, XP003006852
SUGANAMI T. ET AL.: "Himan no Shibo Soshiki ni Okeru Shibo Saibo to Macrophage no paracrine Chosetsukei - Kyobaiyokei o Mochiita Kento -", DAI 26 KAI JAPAN SOCIETY FOR THE STUDY OF OBESITY PROGRAM SHOROKUSHU, vol. 11, no. SUPPLEMENT, 2005, pages 117, XP003006853
TOYODA T. ET AL.: "Shibo Saibo to Macrophage no Sogo Sayo ni Okeru Kakunai Juyotai Agonist no Koensho Sayo", JOURNAL OF JAPAN SOCIETY FOR THE STUDY OF OBESITY 'HIMAN KENKYU', DAI 26 KAI JAPAN SOCIETY FOR THE STUDY OF OBESITY PROGRAM SHOROKUSHU, vol. 11, no. SUPPLEMENT, 2005, pages 172, XP003006854
SUGANAMI T. ET AL.: "Kyobaiyokei o Mochiita Shibo Saibo. Macrophage Sogo Sayo no Bunshi Kiko no Kento", THE JOURNAL OF THE JAPAN DIABETIC SOCIETY, vol. 48, no. SUPPLEMENT 2, 25 April 2005 (2005-04-25), pages S-141, XP003006855
NISHIDA J. ET AL.: "Shibo Saibo. Macrophage Kyobaiyokei ni Okeru Shibo Saibo Enshosei Henka no Bunshi Kiko no Kento", FOLIA ENDOCRINOLOGICA JAPONICA, DAI 78 KAI THE JAPAN ENDOCRINE SOCIETY GAKUJUTSU SOKAI SHOROKUSHU, vol. 81, no. 1, 20 April 2005 (2005-04-20), pages 103, XP003006856
HOTAMISLIGIL G.S. ET AL.: "Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance", SCIENCE, vol. 259, 1993, pages 87 - 91, XP002973111
CLEMENT K. ET AL.: "Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects", FASEB J., vol. 18, 2004, pages 1657 - 1669, XP003006857
WEISBERG S.P. ET AL.: "Obesity is associated with macrophage accumulation in adipose tissue", J. CLIN. INVEST., vol. 112, no. 12, 2003, pages 1796 - 1808, XP003006858
XU H. ET AL.: "Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance", J. CLIN. INVEST., vol. 112, no. 12, 2003, pages 1821 - 1830, XP003006859
Attorney, Agent or Firm:
Yoshida, Yoshiharu (21-19 Toranomon 1-chom, Minato-ku Tokyo, JP)
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