Title:
SECRETION PRODUCTION METHOD FOR PROTEIN
Document Type and Number:
WIPO Patent Application WO/2013/062029
Kind Code:
A1
Abstract:
This invention develops a novel technology that improves the secretion production of a heterologous protein using coryneform bacteria, and provides a secretion production method for the heterologous protein. A coryneform bacteria is cultivated, and a heterologous protein is secreted and produced. The coryneform bacteria has a genetic construct including: a promoter sequence functioning using the coryneform bacteria; a nucleic acid sequence that codes a signal peptide that functions using the coryneform bacteria, connected downstream from the promoter sequence; and a nucleic acid sequence that codes a fusion protein of a heterologous protein and an amino acid sequence including a Gln-Glu-Thr, connected downstream from the nucleic acid sequence coding the signal peptide.
More Like This:
Inventors:
TSURUI NORIKO (JP)
ITAYA HIROSHI (JP)
KIKUCHI YOSHIMI (JP)
ITAYA HIROSHI (JP)
KIKUCHI YOSHIMI (JP)
Application Number:
JP2012/077545
Publication Date:
May 02, 2013
Filing Date:
October 25, 2012
Export Citation:
Assignee:
AJINOMOTO CO., INC. (15-1, Kyobashi 1-chome Chuo-k, Tokyo 15, 〒1048315, JP)
International Classes:
C12P21/02; C12N15/09; C12R1/13; C12R1/15
Domestic Patent References:
| WO2001023591A1 | 2001-04-05 | |||
| WO2002081694A1 | 2002-10-17 | |||
| WO2004029254A1 | 2004-04-08 | |||
| WO2001023591A1 | 2001-04-05 | |||
| WO2005103278A1 | 2005-11-03 |
Foreign References:
| JP2002291476A | 2002-10-08 | |||
| JPH06502548A | 1994-03-24 | |||
| US4965197A | 1990-10-23 | |||
| JPH06502548A | 1994-03-24 | |||
| JP4320769B2 | 2009-08-26 | |||
| JPH11169182A | 1999-06-29 | |||
| JP4362651B2 | 2009-11-11 | |||
| JP4730302B2 | 2011-07-20 | |||
| JPH11341991A | 1999-12-14 | |||
| JP2000316579A | 2000-11-21 | |||
| US6303383B1 | 2001-10-16 | |||
| JPH057491A | 1993-01-19 | |||
| JPH10108675A | 1998-04-28 | |||
| JPH03210184A | 1991-09-13 | |||
| JPH0272876A | 1990-03-13 | |||
| US5185262A | 1993-02-09 | |||
| JPH01191686A | 1989-08-01 | |||
| JPS58192900A | 1983-11-10 | |||
| JPS57134500A | 1982-08-19 | |||
| JPS5835197A | 1983-03-01 | |||
| JPS57183799A | 1982-11-12 | |||
| JPH09322774A | 1997-12-16 | |||
| JP2008271973A | 2008-11-13 |
Other References:
SALIM K. ET AL.: "Heterologous expression of the Mycobacterium tuberculosis gene encoding antigen 85A in Corynebacterium glutamicum", APPL. ENVIRON. MICROBIOL., vol. 63, no. 11, 1997, pages 4392 - 4400, XP002333095
See also references of EP 2772546A4
MICROBIOL. REV., vol. 57, 1993, pages 109 - 137
BIOTECHNOL., vol. 11, 1993, pages 905 - 910
BIOTECHNOL., vol. 6, 1988, pages 1419 - 1422
BIOTECHNOL., vol. 9, 1991, pages 976 - 981
J. BACTERIOL., vol. 174, 1992, pages 1854 - 1861
APPL. ENVIRON. MICROBIOL., vol. 61, 1995, pages 1610 - 1613
APPL. ENVIRON. MICROBIOL., vol. 63, 1997, pages 4392 - 4400
MOL. MICROBIOL., vol. 9, 1993, pages 97 - 109
J. BIOTECHNOL., vol. 112, 2004, pages 177 - 193
INT. J. SYST. BACTERIOL., vol. 41, 1991, pages 255
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, 1998, pages 5511 - 5515
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
DATSENKO, K.A; WANNER, B.L., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 6640 - 6645
CHO, E.H.; GUMPORT, R.I.; GARDNER, J.F., J. BACTERIOL., vol. 184, 2002, pages 5200 - 5203
"Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
GOLDSTEIN ET AL.: "Prokaryotic promoters in biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128, XP008038474
AGRIC. BIOL. CHEM., vol. 48, 1984, pages 2901 - 2903
AGRIC. BIOL.CHEM., vol. 48, 1984, pages 2901 - 2903
GENE, vol. 39, 1985, pages 281 - 286
BIO/TECHNOLOGY, vol. 7, 1989, pages 1067 - 1070
SAITO; MIURA, BIOCHEM. BIOPHYS. ACT., vol. 72, 1963, pages 619
J BIOTECHNOL., vol. 112, 2004, pages 177 - 193
J. BIOL. CHEM., vol. 253, no. 8, 1978, pages 2769 - 76
See also references of EP 2772546A4
MICROBIOL. REV., vol. 57, 1993, pages 109 - 137
BIOTECHNOL., vol. 11, 1993, pages 905 - 910
BIOTECHNOL., vol. 6, 1988, pages 1419 - 1422
BIOTECHNOL., vol. 9, 1991, pages 976 - 981
J. BACTERIOL., vol. 174, 1992, pages 1854 - 1861
APPL. ENVIRON. MICROBIOL., vol. 61, 1995, pages 1610 - 1613
APPL. ENVIRON. MICROBIOL., vol. 63, 1997, pages 4392 - 4400
MOL. MICROBIOL., vol. 9, 1993, pages 97 - 109
J. BIOTECHNOL., vol. 112, 2004, pages 177 - 193
INT. J. SYST. BACTERIOL., vol. 41, 1991, pages 255
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, 1998, pages 5511 - 5515
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
DATSENKO, K.A; WANNER, B.L., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 6640 - 6645
CHO, E.H.; GUMPORT, R.I.; GARDNER, J.F., J. BACTERIOL., vol. 184, 2002, pages 5200 - 5203
"Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
GOLDSTEIN ET AL.: "Prokaryotic promoters in biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128, XP008038474
AGRIC. BIOL. CHEM., vol. 48, 1984, pages 2901 - 2903
AGRIC. BIOL.CHEM., vol. 48, 1984, pages 2901 - 2903
GENE, vol. 39, 1985, pages 281 - 286
BIO/TECHNOLOGY, vol. 7, 1989, pages 1067 - 1070
SAITO; MIURA, BIOCHEM. BIOPHYS. ACT., vol. 72, 1963, pages 619
J BIOTECHNOL., vol. 112, 2004, pages 177 - 193
J. BIOL. CHEM., vol. 253, no. 8, 1978, pages 2769 - 76
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (Acropolis 21 Building 6th floor, 4-10 Higashi Nihonbashi 3-chome, Chuo-k, Tokyo 04, 〒1030004, JP)
Download PDF:
Claims:
Previous Patent: HIV REPLICATION INHIBITOR
Next Patent: OPTICAL SHEET, SURFACE LIGHT SOURCE DEVICE, AND TRANSMISSIVE IMAGE DISPLAY DEVICE
Next Patent: OPTICAL SHEET, SURFACE LIGHT SOURCE DEVICE, AND TRANSMISSIVE IMAGE DISPLAY DEVICE