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Title:
SECRETION PRODUCTION METHOD FOR PROTEIN
Document Type and Number:
WIPO Patent Application WO/2013/062029
Kind Code:
A1
Abstract:
This invention develops a novel technology that improves the secretion production of a heterologous protein using coryneform bacteria, and provides a secretion production method for the heterologous protein. A coryneform bacteria is cultivated, and a heterologous protein is secreted and produced. The coryneform bacteria has a genetic construct including: a promoter sequence functioning using the coryneform bacteria; a nucleic acid sequence that codes a signal peptide that functions using the coryneform bacteria, connected downstream from the promoter sequence; and a nucleic acid sequence that codes a fusion protein of a heterologous protein and an amino acid sequence including a Gln-Glu-Thr, connected downstream from the nucleic acid sequence coding the signal peptide.

Inventors:
TSURUI NORIKO (JP)
ITAYA HIROSHI (JP)
KIKUCHI YOSHIMI (JP)
Application Number:
JP2012/077545
Publication Date:
May 02, 2013
Filing Date:
October 25, 2012
Export Citation:
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Assignee:
AJINOMOTO CO., INC. (15-1, Kyobashi 1-chome Chuo-k, Tokyo 15, 〒1048315, JP)
International Classes:
C12P21/02; C12N15/09; C12R1/13; C12R1/15
Domestic Patent References:
WO2001023591A12001-04-05
WO2002081694A12002-10-17
WO2004029254A12004-04-08
WO2001023591A12001-04-05
WO2005103278A12005-11-03
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Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (Acropolis 21 Building 6th floor, 4-10 Higashi Nihonbashi 3-chome, Chuo-k, Tokyo 04, 〒1030004, JP)
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Claims: