SECRETORY PROTEIN LIBRARY AND METHOD FOR PRODUCING THE SAME

WIPO Patent Application WO/2000/071580

A1

A method for constructing a secretory protein library which comprises culturing a human cell line in a serum-containing medium, then culturing it in a serum-free medium optionally containing a secretion inducing agent, removing the medium components from the culture supernatant containing secretory proteins by the antibody column chromatography method with the use of a resin to which antibodies against proteins contained in the serum and the medium components are bonded, and fractionating and purifying the secretory proteins originating in the cell by isoelectric fractionation, molecular weight fractionation or hydrophobic fractionation.

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KOJIMA SHIN-ICHI (JP)

PCT/JP2000/003214

November 30, 2000

May 19, 2000

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SUMITOMO PHARMA (JP)
KOJIMA SHIN ICHI (JP)

C07K14/47; (IPC1-7): C07K14/47; C07K1/16; C07K1/18; C12N5/06

WO1999005293A1

1999-02-04

YUKI ISHIDA ET AL.: "Bunshi seibutsugaku kenkyu no tameno tanpaku jikkenhou", JIKKEN IGAKU BESSATSU BIO MANUAL UP SERIES (JAPAN), KABUSHIKI KAISHA YOUDOSHA, 30 October 1998 (1998-10-30), pages 228 - 236, 245-253, XP002946517

Aoyama, Tamotsu (Shiromi 1-chome Chuo-ku, Osaka-shi Osaka, JP)

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