FIELD OF THE INVENTION

[0001] The invention is in the field of the selective extraction of anions from solution.

BACKGROUND

[0002] Selective extraction of specific anions from a solution can be useful in several different contexts, but has been limited in practice by the inability of anion extraction to overcome the Hofmeister bias. In liquid-liquid anion extraction, the Hofmeister bias is a thermodynamic bias that favors the extraction of charge diffuse, weakly hydrated anions from water into an organic medium. Therefore, if the anion which is desired to be extracted is in competition with an a nion which is more charge diffuse and weakly hydrated, selective extraction of the desired anion must overcome the Hofmeister bias.

[0003] One example of a system where two anions are in aqueous solution, and it is desirable to remove one of the anions selectively from the solution, is nuclear waste, where both nitrates and sulfates are in solution. Sulfate separation is desirable to improve vitrification of the nuclear waste, but the Hofmeister bias greatly favors the extraction of nitrate from the solution instead of sulfate.

[0004] Sulfate separation is a lso desirable for waste streams to avoid affecting natural biogeochemical cycles and the metabolisms of living organisms. The uncontrolled release of anthropogenic sulfate can have a significant impact on the environment, such as by increasing Earth's albedo or leading to eutrophication of rivers or lakes.

[0005] Extraction of other anions from a liquid solution in contravention to the usual

Hofmeister bias is also desirable in certain liquid handling and separations situations. Additional anions for preferential extraction may include phosphate, arsenate, chloride, carbonate, fluoride, selenate, selenite, sulfite, chromate, pyrophosphate and arsenite.

[0006] Phosphate, along with sulfate, leads to eutrophication of lakes and rivers,

therefore methods for the specific removal of phosphates, in addition to sulfates, from water entering lakes and rivers are highly sought after, such as removal of phosphates and sulfates widely used in fertilizer from agricultural runoff. Chloride has also been found to accumulate in lakes due to the use of large amounts of road-salt (NaCI, CaCI ₂ ) in the winter, and cause adverse environmental effects. Therefore, preferential removal of chloride from such environments is desirable. Anions such as chromate, arsenate, arsenite, selenate, selenite are highly toxic, therefore they need to be removed very efficiently from waste waters at industrial and mining sites, or water purification plants.

BRIEF SUMMARY

[0007] In one aspect, the present innovation includes an anion-encapsulating compound, including an encapsulating host of the formula c/ ^' s-Cu" _x (ORi) _y (R2pz) _z . Ri is H, CH ₃ , C ₂ H ₅ , C3H7, C ₄ H ₉ or another alkyl group, R ₂ is H, CH ₃ , C ₂ H ₅ , C ₃ H ₇ , C ₄ H ₉ or another a lkyl or charged group, and pz is a pyrazolate anion. Each of x, y, and z is equal to an integer between about 1 and 40, inclusive.

[0008] In another aspect, the present innovation includes a method of selectively

extracting a targeted anion from an aqueous solution. The method includes the steps of combining a solvent and anion-encapsulating components with the aqueous solution containing the targeted anion to form an organic-aqueous mixture. The organic-aqueous mixture has an organic phase and an aqueous phase. The anion-encapsulating

components include a copper ion contributor, a hydroxide ion contributor, and a pyrazole. The anion-enca psulating components are allowed to react to form

encapsulating hosts in situ in the organic-aqueous mixture around the target anions to contain the target ions therein. The target ion is chosen from the group consisting of sulfate, phosphate, arsenate, carbonate, selenate, selenite, sulfite, chromate,

pyrophosphate and a rsenite ions wherein the target anion is chosen from the group consisting of sulfate, phosphate, arsenate, carbonate, selenate, selenite, sulfite, chromate, pyrophosphate and arsenite ions. The organic phase and the aqueous phase are separated.

[0009] In another aspect, the present innovation includes a method of preparing an

encapsulating assembly, including the steps of providing a first encapsulating host, the first encapsulating host including a copper ion contributor, a hydroxide ion contributor and a first pyrazole. A ligand-bound pyrazole is substituted for at least some of the first pyrazole in the encapsulating host to form a modified encapsulating host. The ligand- bound pyrazole comprises an alkyl group bound to a pyrazole group. The modified encapsulating host has a greater solubility in a non-polar solvent than the first encapsulating host. [0010] In another aspect, the present innovation includes a method of preparing an encapsulating assembly, including the steps of providing a first encapsulating host, the first encapsulating host including a copper ion contributor, a hydroxide ion contributor and a first pyrazole. A ligand-bound pyrazole is substituted for at least some of the first pyrazole in the encapsulating host to form a modified encapsulating host. The ligand- bound pyrazole comprises a charged ligand bound to a pyrazole group. The modified encapsulating host has a greater solubility in a polar solvent than the first encapsulating host, altered recognition properties from the first encapsulating host, altered binding properties from the first encapsulating host, or altered tethering properties from the first encapsulating host.

[0011] In another aspect, the present innovation includes a method of preparing an

encapsulating assembly, including the steps of combining a copper ion contributor, a hydroxide ion contributor, and a first pyrazole to form an encapsulating host. A ligand- bound pyrazole is substituted for at least some of the first pyrazole during, after, or during and after formation of the enca psulating host to form a modified encapsulating host. The ligand bound to the ligand-bound pyrazole is a charged ligand or an alkyl group.

[0012] In another aspect, the present innovation includes a method of selectively

extracting a targeted anion from an aqueous solution. The method includes the steps of combining a solvent, a polymer chain of [(Ιυ"(μ-ΟΗ)(μ-ρζ)] _∞ , and an aqueous solution containing the target anion to form an organic-aqueous mixture. The organic-aqueous mixture has an organic phase and an aqueous phase. The polymer chain is allowed to form an encapsulating host around the target anion. The target anion is chosen from the group consisting of sulfate, phosphate, arsenate, carbonate, selenate, selenite, sulfite, chromate, pyrophosphate and arsenite ions. The organic phase is separated from the aqueous phase.

[0013] These and other features, advantages, and objects of the present invention will be further understood and a ppreciated by those skilled in the art by reference to the following specification, claims, and appended drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

[0014] FIG. 1 is a top view of sulfate enca psulated in an encapsulating host, represented by the formula S04¾c/ ^' s-Cu ^l -OH)^-pz)} ₆₊ i ₂₊ io (SO^ ^' cicis-Cu' -OHj^-pzJhe); [0015] FIG. 2 is a side pla n view of the sulfate encapsulated in a n encapsulating host, represented by the formula as shown in FIG. 1;

[0016] FIG. 3 is a side pla n view showi ng the formation of the sulfate-encapsulating

na no-jar;

[0017] FIG. 4 is a top view showi ng the formation of the sulfate-encapsulating "nano- ja r,";

[0018] FIG. 5 is a transmission electron micrograph (TEM) image of sulfate encapsulating assemblies deposited onto a copper grid by the evaporation of a solution in ethanol;

[0019] FIG. 6 is a side pla n view showi ng the formation of the chromate-encapsulati ng

"nano-ja r";

[0020] FIG. 7 is a side pla n view of an a rsenate-encapsulating compound, where H atoms are omitted for cla rity; and

[0021] FIG. 8 is a side pla n view of the phosphate enca psulating assembly, where H

atoms are omitted for clarity.

DETAI LED DESCRI PTION

[0022] Various example embodiments a re described herein. However, it is to be

understood that the i nvention may assume various alternative embodiments or orientations except where expressly specified to the contra ry. It is a lso to be understood that the specific embodiments a nd processes il lustrated in the attached drawings and described in the following specification a re simply exemplary and that those skilled in the art will be a ble to substitute va rious a lternative components or counterions, or use alternative solvents, pH-adjusting com pounds or other components without affecting the functional structure of the a nion-encapsulating aggregates described herein or the method of removal of the a nions from solution. Hence, specific methods of synthesis, dimensions a nd other physical characteristics relating to the embodiments disclosed herein a re not to be considered as limiting. Throughout the disclosure, relevant terms are to be understood consistently with their typical meanings in the relevant art, unless otherwise defined herein.

[0023] Certain embodiments of a nion-enca psulating aggregates based on a series of compounds a re described herein, as well as methods of using the anion-encapsulating aggregates to remove targeted a nions from solution. I n one embodiment, the a nion- encapsulating aggregate is based on an encapsulating host, where the host has the formula c/ ^' s-Cu" _x (ORi) _y (R ₂ pz) _z , where Ri is H, CH ₃ , C ₂ H ₅ , C ₃ H ₇ , C ₄ H ₉ or another alkyl group, R ₂ is H, CH3, C2H5, C ₃ H ₇ , C ₄ H ₉ or another alkyl or charged group, pz is a pyrazolate anion, and each of x, y, and z is equal to an integer between about 1 and 40, inclusive.

[0024] One preferred set of embodiments includes anion-encapsulating aggregates

based on a series of compounds of the formula c/ ^' s-Cu" _x (OR) _y (pz) _z , where R is H, CH ₃ , or another alkyl group, pz is pyrazolate anion and each of x, y, and z is equal to an integer between about 1 and about 40, inclusive. Another particular embodiment of this compound is a cyclic polymerization isomer of the formula [<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)] _η , where n is an integer between about 6 and about 16, inclusive. As used herein, μ means

"bridging," meaning that where " μ-ΟΗ" is used, the O atom of the OH group bridges two adjacent copper centers. Similarly, where "μ-ρζ" is used, the two N atoms of the pyrazolate ligand are bound to two different copper atoms, and therefore the pyrazolate ligand is bridging two adjacent copper centers. Where "μ ₃ -ΟΗ" is used below, it means that the O atom of the OH group bridges three adjacent copper centers.

[0025] Such anion-encapsulating aggregates can be used to selectively extract target anions of sulfate, phosphate, arsenate, carbonate and chromate anions preferentially from a solution, and are also believed to be useful in separating selenate, selenite, sulfite, pyrophosphate, and arsenite anions preferentially from a solution.

[0026] The anion host assemblies described herein, form "nano-jars" or toroids of the host around the anion to be extracted in solution, as further described below. The anion host assemblies exhibit a large number of hydrogen-bonds between the targeted anion and the rings and strands of the host assemblies, resulting in encapsulation of the targeted anion by the host assemblies, and sequestration of the targeted anion, an example of which is shown in FIGS. 1-2.

[0027] FIG. 1 is a top view of S0 ₄ ^2~ c{c/ ^' s-Cu'V-OH)^-pz)}6+i2+io, where 6-member

pyrazole ligand rings, 12-member pyrazole ligand rings, and 10-member pyrazole ligand rings form a 29-member pyrazole ligand "nano-jar" 10 a round the target anion 12. FIG. 2 is a side plan view of the encapsulating host which forms the nano-jar 10 and the target anion 12.

[0028] As shown in FIGS. 3-4, a suitable counterion, when added to the solution of nano- jar 10 forming host assemblies in certain solvent environments, further associates with the host assembly to form a lid 14 for the nano-jar 10 to complete the anion- encapsulating aggregate. One set of suitable counterions include large cations. Potential large counterions include, but are not limited to counterions such as

tetrabutylammonium (Bu ₄ N ⁺ ), tributylammonium (Bu ₃ NH ⁺ ), tetraethylammonium (Et ₄ N ⁺ ), triethylammonium (Et ₃ NH ⁺ ), 18-crown-6 potassium complex (K ⁺ -18C6), tris(l,10- phenanthroline)copper(l l) - [Cu(phen) ₃ ] ²⁺ , and

bis(triphenylphosphoranylidene)ammonium (Ph ₃ P=N=PPh ₃ ⁺ ). Additional cation counterions can be used to form the lid for the nano-jar, including smaller alkali or alkaline-ea rth metal cation counterions. Non-limiting examples of such smaller counterions include sodium, potassium, rubidium, cesium, and barium ions.

[0029] However, the attachment of the lid 14 to the na no-jar 10 is not required for

selective anion extraction. The nano-jars 10 described herein self-assemble around the particular ta rgeted anion 12 during the extraction process. The lid 14 will generally be attached to the nano-jar 10 if the solvent is fairly non-polar (such as chloroform), but will generally be detached if the solvent is polar (such as dimethylsulfoxide). The binding strength of the nano-jars 10 toward target anions 12 is independent of the lid 14.

Addition of the lid 14 creates a compound which is soluble in certain solutions.

[0030] To perform a liquid-liquid extraction to extract a target anion from an aqueous solution using the anion host assemblies as described herein, one preferred method is to combine a solvent and the nano-jar components, including a copper salt, pyrazole, and a base, with an aqueous solution containing the anion(s) to be extracted to form an organic-aqueous mixture. The solvent and nano-jar components can be added together

(with the com ponents dissolved or suspended in the solvent, or with one or more of the components combined with the solvent or each other), or can be added individua lly in any order to the aqueous solution to form the organic-aqueous mixture, having an organic phase and an aqueous phase. The components in the organic-aqueous mixture are then permitted to react. During such reaction, the encapsulating host assemblies begin forming in situ in the organic-aqueous mixture, around the target anions, even if other competing anions which would normally be favored by the Hofmeister bias are present in the aqueous phase. As the nano-jars form, they transfer to the organic phase.

The organic phase and aqueous phase can then be separated using known separation techniques therefor, such as a separatory funnel. Upon separation, the target anions and encapsulating host assemblies are removed in the organic phase, leaving an aqueous phase from which some or all of the target anions have been removed.

[0031] A compound containing or capable of providing a suitable counterion upon

reaction, a counterion contributor, is a lso added to the organic-aqueous mixture to act as the lid for the nano-ja rs and to complete an anion-encapsulating aggregate. If bases such as triethylamine or tributylamine a re used, there is no need to add a separate counterion contributor because the triethylammonium or tributylammonium ions resulting from the self-assembly reaction (by protonation of the base) will serve as suitable counterion lids. The solvents used in addition to the nano-jar components are believed to have little or no influence on the self-assembly process of the "nano-jars." If the extraction is performed without a suitable counterion, the reaction may lead to an insoluble materia l, hampering removal of the target anion from the aqueous phase of the liquid. Illustration of the addition of the lid to an encapsulated target anion is shown in FIG. 4.

[0032] Suitable copper salts may include copper nitrate, copper perchlorate, copper tetrafluoroborate, or other copper salts having copper combined with another anion having a small hydration energy, which contribute a copper ion for formation of the anion-encapsulating assembly. Suitable bases include, but are not limited to, bases such as potassium hydroxide, sodium hydroxide, triethylamine, tributylamine,

tetrabutylammonium hydroxide, and trialkylamenes, all of which contribute or cause the contribution of a hydroxide ion for the formation of the anion-encapsulating assembly.

[0033] Alternatively, instead of adding a copper salt and a base, Cu(OH) ₂ can be added to the solution to replace the copper salt and the base listed above. The Cu(OH) ₂ behaves as a copper-ion contributor (as the copper salt does), and also as a hydroxide ion

contributor (as the base does). The pyrazole used to form the anion-encapsulating assembly optionally includes an alkyl group ligand bound thereto. Suitable solvents may include toluene, tetrahydrofura n, chloroform, dichloromethane or other solvents. To facilitate separation of the organic and aqueous phases of the liquid, the solvent used cannot be miscible with water under the final conditions of the reaction (when the anion- encapsulating hosts and target anions a re present in the organic phase). For example, despite the fact that tetrahydrofuran (THF) is normally miscible with water, the THF will form a phase separate from the aqueous phase solution at high ionic strengths. [0034] Another method for performing a liquid-liquid extraction of a target anion is to add a solvent, a polymer chain of [(Ιυ"(μ-ΟΗ)(μ-ρζ)] _∞ and a suitable counterion contributor to an aqueous solution containing the targeted anion for extraction, to form an organic-aqueous mixture. The com ponents in the organic-aqueous mixture are then permitted to react. Upon such reaction, the nano-jars self-assemble around the target anions and transfer into the organic phase, selectively removing the target anion from the aqueous phase. Separation of the organic phase from the aqueous phase results in extraction of some or all of the targeted anion from the aqueous phase.

[0035] As described above, the solvent, polymer chain, and counterion contributor can be added together (with the components dissolved or suspended in the solvent, or with one or more of the components combined with the solvent or each other), or can be added individually in any order to the aqueous solution to form the organic-aqueous mixture. Suitable solvents and counterions for such reaction are the same as those described above.

[0036] Including certain metal ions during the formation of the nano-jars also influences formation of the anion-encapsulating aggregates and anion host assemblies, resulting in the formation of specific anion host assemblies. Metals which are preferred for influencing the formation of the nano-jars include without limitation lead, calcium, cobalt, manganese, praseodymium, neodynium, zinc, cadmium, and nickel. To use such metals to influence the formation of the anion host assemblies or anion-encapsulating aggregates, metal ions or compounds capable of contributing the metal ions are added to the solution while the anion-encapsulating aggregates are forming.

[0037] As used throughout the following description, unless stated otherwise in a

particular instance, the formulas given for specific anion-encapsulating host assemblies and aggregates will be the formula of the solid compound that is present upon drying of the solvent.

[0038] One preferred embodiment of an anion-encapsulating aggregate is based on the three-ring {<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)}6 ₊ ΐ2+ιο enca psulating host assembly ("Host 1"), as shown in FIG. 1 and FIG. 2. Host 1 can a lso be denoted as {<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)}28, and is one ring set that is representative of {<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)}28· The two opposite sides of the 6+12 membered ring assembly in this host assembly are curved upwardly, indicating the flexibility of the Cu-rings. Another preferred embodiment is based on the three-ring enca psulating host assem bly ("Host 2"). Similar to Host 1, Host 2 can a lso be denoted as {<;/ ^' 5-(Ιυ"(μ-ΟΗ)(μ-ρζ)} _3ΐ , a nd is one ring set that is representative

[0039] Host 1 a nd Host 2 show selectivity for the encapsulation of sulfate ions over

nitrate and perchlorate ions, and are believed to a llow selective extraction of sulfate in the presence of chlorate, bromate, iodate, periodate, cyanate, trifluoromethansu lfonate, permanganate, perrhenate, pertechnetate, picrate, tetrafluoroborate,

tetraphenylborate, hexafluorophosphate a nd other anions that have a Gibbs free energy of hyd ration smaller tha n ~310 KJ/mol. Partial selectivity for the sulfate ion by Host 1 and Host 2 has also been observed in the presence of fluoride, chloride and bromide ions.

[0040] Host 1 and Host 2 each exhibit a large numbe r of hydrogen-bonds between the sulfate anion and the two smal ler rings in each assembly. This enca psulation of the sulfate by neutra l hydrogen bond donors is on par with sulfate-binding proteins occurring in natu re that sequester sulfate anions.

[0041] The encapsulating host assemblies in Host 1 and Host 2 include nano-jars of {cis-

Cu"(μ-OH)(μ-pz)} _6+l 2 _+l o a nd { ^' -Cu"(μ-OH)(μ-pz)} _8+l4+9 , respectively, which serve to enca psulate the sulfate ions, forming the compounds S0 ₄ ^2~ c{c/s-Cu' -OH)^-pz)}6 ₊ i ₂₊ io

(Γυ"(μ-ΟΗ)(μ-ρζ)} ₃ ι, respectively. As used herein, the symbol c means that the a nion which precedes the sym bol is enca psu lated withi n the ring structure which is annotated after the sym bol.

[0042] One exa mple of a suita ble counterion for forming the lid over the sulfate- enca psulating host assemblies is Bu ₄ N ⁺ , resulting in anion-enca psulating aggregates

(Βυ ₄ Ν) ₂ [$0 ₄ ¾^υ^μ-ΟΗ)(μ-ρζ)} ₆₊₁₂₊ ^

in the above example.

[0043] The unusually high solubi lity of (Βυ ₄ Ν) ₂ [50 ₄ ^2" ^{θ -αυ"(μ-ΟΗ)(μ-ρ ^ζ )} ₆₊ ΐ2 ₊ ιο], and

(Βυ ₄ Ν) ₂ [50 ₄ ^2" ^{θ -αυ"(μ-ΟΗ)(μ-ρζ)} ₈₊ ι ₄₊₉ ] (>20 g in 100 ml CH ₂ CI ₂ ), as well as their solubi lity in non-pola r solvents (such as CS ₂ , CCI ₄ and benzene), indicate the existence of these hydrophobic nano-jars in solution (similar to the ones seen in the crysta l structure), resulting from the association of the sulfate-enca psulating na no-jars 10 with the Bu ₄ N ⁺ "lids" 14 as shown in Figs. 3 and 4. [0044] Preliminary selectivity studies indicate a marked preference of the [c/s-Cu' -

ΟΗ)(μ-ρζ)] _η assemblies of Host 1 and Host 2 described herein for the sulfate anion over nitrate or perchlorate anions. A self-assembly reaction using a 1:54 molar ratio of sulfate and nitrate (or perchlorate) anions led to the qua ntitative encapsulation of sulfate into the product, as determined gravimetrically by precipitation as BaS0 ₄ after digestion with 6M HCI. The preference for the anion with larger free energy of hydration (S0 ₄ ^2~ : -1090 kJ/mol; N0 ₃ ^~ : -306 kJ/mol; CI0 ₄ ^~ : -214 kJ/mol) is known as anti-Hofmeister bias, and indicates that the norma lly observed Hofmeister bias does not control the encapsulation reaction. The selectivity for sulfate over nitrate, along with the stability at high (basic) pH is especially relevant for the sulfate-extraction efforts during nuclear waste treatment by vitrification, because it allows liquid-liquid extraction. The methods described herein also offer the benefit of recyclability of the sulfate-encapsulating assemblies. The

encapsulating host assemblies can be recovered by stirring the dry residue obtained after BaS0 ₄ precipitation (containing CuCI ₂ , Hpz and Et ₃ NHCI) with excess Et ₃ N in DMF.

Following such mixing, the cha racteristic a bsorption at 599 nm indicates the re-assembly of the [<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)] _η rings.

[0045] To use Host 1 and Host 2 for sulfate extraction, a solvent, the stoichiometrica lly required amounts (based on the amount of sulfate to be extracted) of nano-jar ingredients of copper salt, a base (or copper hydroxide in place of the copper salt and base) and pyrazole, and a counterion contributor (if the base does not act as a counterion contributor) are combined with an aqueous solution containing sulfate ions, which may or may not be in the presence of competing ions such as nitrate, to form an organic- aqueous mixture, as described above. I n other words, for sulfate extraction, a solvent, a copper contributor, a hydroxide contributor, a counterion contributor, and pyrazole are combined with the aqueous solution containing the targeted sulfate anions to form the organic-aqueous mixture. The copper contributor, hydroxide contributor and counterion contributor may be three separate compounds, or one compound may perform as a contributor of more than one of copper, hydroxide and counterions.

[0046] The components in the organic-aqueous mixture are permitted to react, after which the sulfate is encapsulated into the Host 1 and Host 2 ring assemblies, which are found in the organic phase of the organic-aqueous mixture. The organic layer of organic- aqueous mixture can then be separated from the aqueous layer using known separation techniques, such as the use of a separatory funnel.

[0047] Alternatively, to use Host 1 a nd Host 2 for sulfate extraction, a solvent, a polymer chain of [(Ιυ"(μ-ΟΗ)(μ-ρζ)] _∞ and a counterion contributor are com bined with an aqueous solution containing sulfate ions to form an organic-aqueous mixture, as described above. The components in the organic-aqueous mixture are permitted to react, after which the sulfate is encapsulated into the Host 1 and Host 2 ring assemblies, which are found in the organic phase of the organic-aqueous mixture. The organic layer of organic-aqueous mixture can then be separated from the aqueous layer using known separation techniques, such as the use of a separatory funnel.

[0048] In experiments, an aqueous solution containing 5 M nitrate (NaN0 ₃ ), 0.044 M sulfate (Na ₂ S0 ₄ ) and 1.25 M NaOH (pH 14), in which the sulfate to nitrate ratio is 1:114 was combined together with an organic phase containing the stoichiometrically (based on the amount of sulfate) required amounts of nano-jar components. The solution effectively and selectively extracted the sulfate anions from the aqueous phase to the organic phase, where they were encapsulated in the Host 1 and Host 2 "nano-jars." Sulfate extraction can also be performed at high dilutions, such as 20 mg of sulfate per liter, using toluene as a solvent.

[0049] Host 1 and Host 2 ca n be prepared on large scale via either depolymerisation of

[(Ιυ"(μ-ΟΗ)(μ-ρζ)]∞ ₀ r a one-pot self-assembly reaction from its basic constituents in the presence of a sulfate ion.

[0050] Production of Host 1 and Host 2 via depolymerisation of [(Ιυ"(μ-ΟΗ)(μ-ρζ)]∞

includes the steps of combining aqueous solutions of CuS0 ₄ and KOH/Hpz (1:2:1 molar ratio), which results in the formation of a blue-purple precipitate (Scheme 1). The insolubility of this materia l in all common organic solvents, including refluxing pyridine (b.p. = 115 °C) and DM F (b.p. = 153 °C), suggests a polymeric structure. Elemental analysis results concur with the formula [Cu(OH)(pz)] (for C ₃ H ₄ CuN ₂ 0, M.W. = 147.62, calculated/found: C, 24.41/24.25%; H, 2.73/2.72%; N, 18.98/18.40%). The structure of this intractable material likely consists of linear polymeric chains resulting from an all- irons arrangement of the pyrazole and OH moieties about the copper centers, similar to the ones found in

[0051] (Bu ₄ N)HS0 ₄ or (Bu ₄ N) ₂ S0 ₄ can be added to a suspension of [(Ιυ(μ-ΟΗ)(μ-ρζ)]∞ in

THF or toluene, which leads to the immediate formation of a deep blue solution, and gradual dissolution of the solid. Evaporation of the solvent produces a dark blue powder, which is highly soluble in a wide variety of solvents (including CCI ₄ , CS ₂ , aromatic hydrocarbons, ethers, ketones, esters, nitriles, amines, halogenated and nitro

compounds, DM F and DMSO), slightly soluble in alcohols, and insoluble in aliphatic hydrocarbons and water. I n CH ₂ CI ₂ or DM F solution, it exhibits an absorption maximum at 599 nm, virtually identical to the one of [Cu(NH ₃ ) ₄ (H ₂ 0) ₂ ] ²⁺ in H ₂ 0 (600 nm), and a molar absorptivity of ~2.9 χ 10 ³ L mol ¹ cm ^"1 . Hexa ne vapor diffusion to a toluene solution is then used to obtain dark blue single crystals in the form of blocks and thin plates. X-ray diffraction analysis revealed their structures to be (Bu ₄ N) ₂ [S0 ₄ ^2~ c{c s-Cu"^-OH)^-pz)} ₂₈ ], and (Bu ₄ N) ₂ [S0 ₄ ^2" c{c s-Cu"^-OH)^-pz)} _3i ], respectively.

[0052] To synthesize Host 1 and Host 2 via a direct self-assembly reaction, several

potential self-assembly reactions are possible. As a first option, the constituents of CuS0 ₄ »5H ₂ 0, KOH, Hpz a nd (Bu ₄ N) ₂ S0 ₄ (28:56:28:1 molar ratio) can be added to various solvents (THF, CH ₂ CI ₂ or DM F). Alternatively, CuS0 ₄ -5H ₂ 0, KOH, pyrazole and Bu ₄ NOH (in water) can be combined in a THF solution and mixed to form a solution, from which the product can be separated upon filtration and evaporation of the solvent. Alternatively, a solution of CuS0 ₄ -5H ₂ 0 in DM F can be com bined with a solution of KOH, pyrazole and Bu ₄ NOH (in water) in methanol, and the resulting solution poured into water, to produce a precipitate of the product. Alternatively, CuS0 ₄ -5H ₂ 0, KOH, pyrazole and Bu ₄ NOH (in water) can be added to CH ₂ CI ₂ , to produce a solution, from which the product can be separated upon filtration and evaporation of the solvent. The product in each of these cases includes (Bu ₄ N)2[S0 ₄ ^2" c{c s-Cu"^-OH)^-pz)} ₂ 8] and (Bu ₄ N)2[S0 ₄ ^2" c{c/ -Cu'V- ΟΗ)(μ-ρζ)} ₃ ι], as evidenced by mass spectrometric analysis (electrospray ionization).

[0053] (Bu ₄ N)2[S0 ₄ ^2" c{c s-Cu"^-OH)^-pz)} ₂ 8] can also be crystallized from

chlorobenzene, 1,2-dichloroethane or n-butylamine by hexane vapor diffusion, and from CH ₂ CI ₂ by Et ₂ 0 vapor diffusion. can also be crystallized as the Bu ₄ N-salt from chlorobenzene. Moreover, crystals of

pz)} ₂₈ ] were also obtained by recrystallization from boiling n-butanol (b.p. = 118 °C), indicating stability of the rings at that temperature.

[0054] Addition of the various counterions (e.g., 18-crown-6, Et ₃ N) results in the

formation of nano-jars with alternate "lids." For example, CuS0 ₄ »5H ₂ 0, KOH, pyrazole and 18-crown-6 (28:56:28:2 molar ratio) can be added to THF to produce (K ⁺ cl8-crown- 6)2[S0 ₄ ^2" c{cis-Cu' -C>H)^-pz)}28], and (K ⁺ cl8-crown-6)2[S0 ₄ ^2~ c{cis-Cu'V-OH)^-pz)} ₃ i] . These nano-jars form in the organic solution, and can be isolated by evaporation of the solvent or, in the case of organic solvents which are miscible with water, by the addition of water to form a precipitate of the product. can also be crystallized as the K ⁺ 18-crown-6-salt from n-butyl acetate, by hexane vapor diffusion. Alternatively, CuS0 ₄ »5H ₂ 0, pyrazole and Et ₃ N (1:1:2 molar ratio) can be added to DMF, to produce a precipitate of (Et ₃ NH)2[S0 ₄ ^2~ c{cis-Cu' -OH)^-pz)}28] and (Et ₃ NH) ₂ [S0 ₄ ^2~ (jn quantitative yield) upon addition of water.

[0055] The counterions and solvents used apparently have no influence on the self- assembly process of the anion-encapsulating host, since only {<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)}28 and {<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)} ₃ ι resulted from those reactions. Running the reaction without a suitable counterion (e.g., using CuS0 ₄ »5H ₂ 0, KOH and Hpz only), however, led to an insoluble material.

[0056] Host 1 and Host 2 a re both formed during the synthesis steps described herein, and coexist in the same solution. Host 1 and Host 2 can be separated upon crystallization due to the visual differences in the crystals (Host 1 forms dark blue prismatic crystals and Host 2 forms dark purple-blue plate-like crystals). FIG. 5 includes a transmission electron micrograph (TEM) image of sulfate encapsulating assemblies, or nano-jars 10, deposited onto a copper grid by the evaporation of a solution in ethanol showing the crystals. Other synthesis procedures may allow for the preferential formation of one of Host 1 or Host 2. However, as both are well suited to sulfate ion extraction, coexistence in the same solution does not negatively impact the selective extraction of sulfate ions.

[0057] Another preferred embodiment of an anion-enca psulating aggregate is based on the four-ring encapsulating host assembly ("Host 3"). Host 3 can a l and is one ring set that is representative of ectivity for the encapsulation of phosphate and a rsenate anions from a solution containing com peting ions where the competing ions would normally be favored for extraction into an organic phase by the Hofmeister bias. Host 3, like Hosts 1 and 2, results in the wrapping of the target ions 12 within a nano-jar structure 10, which selectively binds the targeted anions, as shown in FIG. 8.

[0058] As with the separation methods for sulfates described above, to selectively

separate phosphate or arsenate anions from an aqueous solution, a solvent, the components stoichiometrically (based on the amount of phosphate and/or arsenate) necessary to create the Host 3 na no-ja r, and a counterion contributor (if the base does not act as such) are combined with the aqueous solution containing the target anions to form an organic-aqueous mixture, and the combined components are permitted to react. Upon such reaction, the nano-jars self-assemble around the target anions and transfer into the organic phase, selectively removing phosphate or arsenate from the aqueous phase, even in the presence of other anions which would be favored by the Hofmeister bias.

[0059] Alternatively, a solvent, a polymeric chain of [(Γυ"(μ-ΟΗ)(μ-ρζ)] _∞ and a counterion contributor can be combined with an aqueous solution containing the targeted anion for extraction to form an organic-aqueous mixture, and permitted to react. Upon such reaction, the na no-jars self-assemble around the target anions and transfer into the organic phase, selectively removing phosphate or arsenate from the aqueous phase. Separation of the organic phase and aqueous solution results in extraction of some or all of the targeted anions from the aqueous solution, even in the presence of other anions which would be favored by the Hofmeister bias.

[0060] To synthesize Host 3, several methods may be employed. First, Cu(OH) ₂ can be prepared by mixing aqueous solutions of CuS0 ₄ -5H ₂ 0 with KOH 85%. A precipitate forms, which is preferably washed with water and then THF and dried. The dried solid can then be added to a solution of pyrazole and tetrabutylammonium phosphate in

tetrahydrofuran, forming a solution with a deep blue color. Upon filtration and evaporation, a dark blue powder is obtained, containing a four ring (3, 6, 9 and 12 Cu- pyrazole rings) encapsulating host assembly encapsulating phosphate anions of the formula or (Bu ₄ N)[P0 ₄ ^3~ The same encapsulating host assembly can be synthesized in the presence of an a rsenate ion, to encapsulate the arsenate ion rather than the phosphate ion, resulting in (Bu ₄ N)[As0 ₄ ^3~ c{cis-Cu'V-OH)^-pz) } ₆₊ ι ₂₊₉ {(Ιυ" ₃ (μ ₃ -ΟΗ)(μ- pz) ₃ }] or (Bu ₄ N)[ As0 ₄ ¾cis-Cu" ₃ o^-OH) ₂₈ ^-pz) ₃₀ }].

[0061] In a laboratory scale synthesis, a solution of 968 mg CuS0 ₄ -5H ₂ 0 in 20 mL H ₂ 0 was mixed with a solution of 512 mg KOH 85% in 20 ml H ₂ 0. The precipitate that formed upon mixing was washed extensively with water, and then with THF. The dried precipitate was then added to a solution of 264 mg pyrazole and 200 mg

tetrabutylammonium phosphate in 15 ml tetrahydrofuran. After stirring for 2 days, the deep blue solution was filtered and the solvent was evaporated. 684 mg dark blue powder was obtained.

[0062] Another method of synthesis of Host 3 includes combining Cu ₂ (OH)(P0 ₄ ), KOH

85%, pyrazole and Bu ₄ NOH solution (1 M in water) and stirring, to form a solution. Upon filtration and evaporation of the solution, a dark blue powder containing (Bu ₄ N)[P0 ₄ ^3~ was obtained.

[0063] In a laboratory scale synthesis, 464 mg Cu ₂ (OH)(P0 ₄ ), 356 mg KOH 85%, 264 mg pyrazole and 460 mg Bu ₄ NOH solution (1 M in water) were stirred together for 2 days. The deep blue solution which formed was filtered and the solvent was evaporated. 548 mg dark blue powder was obtained.

[0064] Another method of synthesis of Host 3 includes dissolving Cu(N0 ₃ ) ₂ -2.5H ₂ 0 in dimethylformamide, and then adding a solution of NaOH, pyrazole, H ₃ P0 ₄ 85% and Bu ₄ NOH solution (1 M in water) in methanol under vigorous stirring. After stirring, the solution is added to water, forming a precipitate. The precipitate can be filtered out of solution, washed with water, and dried in an oven, forming a dark blue powder containing (Bu ₄ N)[P0 ₄ ¾cis-Cu ^M ₃ o(^-OH) ₂₈ ^-pz) ₃₀ }]. [0065] In a laboratory scale synthesis 5.000 g Cu(N0 ₃ )2-2.5H ₂ 0 was dissolved in 167 m l dimethylformamide. A solution of 1.720 g NaOH, 1.464 g pyrazole, 83 mg H ₃ P0 ₄ 85% a nd 0.72 m l Bu ₄ NOH solution (1 M in water) in 33 ml methanol was added in 1-2 ml portions under vigorous stirring. After stirring for 3 days, the solution was poured into 500 ml water. The precipitate was filtered out, washed with water and dried in a n oven at 80 °C. 3.727 g da rk blue powder was obta ined.

[0066] Another method of synthesis of Host 3 includes the steps of reacting [(Ιυ(μ-ΟΗ)(μ- pz)] _∞ polymer with a solution of tetrabutylammonium phosphate in chlorobenzene. The solution is then filtered, and the solvent evaporated, to obtain a da rk blue powder containing (Βυ ₄ Ν) [Ρθ4 ^3" α{οϊ5-αυ ^ΙΙ 3ο(μ-ΟΗ)28(μ-ρ ^ζ ) ₃ ο}] .

[0067] In a laboratory scale synthesis 500 mg [Cu" (μ-ΟΗ)(μ-ρζ)] _∞ polymer was refluxed with 200 mg tetrabutyla mmonium phosphate in 15 ml chlorobenzene for 2 hours. The solution was filtered and the solvent was evaporated. 430 mg da rk blue powder was obtained.

[0068] Another preferred anion-enca psulating aggregate is based on the Cui ₅ compound, a {Cu"3^3-OH)^-pz)3}2{Cu"3^-OH)2^-pz) ₄ }3 host assem bly ("Host 4"), which selectively enca psulates two phosphate or two a rsenate anions, as shown in FIG. 7. Host 4 ca n also be denoted as { a nd is one ri ng set that is representative of { cis- (Γυ"ι ₅ (μ-ΟΗ) ₇ (μ-ρζ)ΐ8}. Host 4 is formed at the same time and in the same reactions as Host 3, as described a bove, and is present when phosphate or arsenate are present in the aqueous solution for extraction.

[0069] As with the separation methods described a bove, when selectively separating phosphate or arsenate anions from a n aqueous solution, a solvent, the components necessary to create the Host 4 compound a nd a counterion contributor (if the base does not act as such) a re com bined with the aqueous solution containing the target anions to form an orga nic-aq ueous mixtu re, and the combined components are permitted to react. Upon such reaction, the com pounds comprising Host 4 self-assemble a round the ta rget anions and tra nsfer into the organic phase, selectively removing phosphate and/or arsenate anions from the aqueous phase, even in the presence of other anions which would be favored by the Hofmeister bias. [0070] Alternatively, a solvent, a polymeric chain of [Cu (μ-ΟΗ)(μ-ρζ)] _∞ and a counterion contributor can be combined with an aqueous solution containing the targeted anion for extraction to form an organic-aqueous mixture and permitted to react. Upon such reaction, the Host 4 compound self-assembles around the target anions and transfers into the organic phase, selectively removing phosphate or arsenate from the aqueous phase. Separation of the organic phase and aqueous solution results in extraction of some or all of the targeted anions from the aqueous solution, even in the presence of other anions which would be favored by the Hofmeister bias.

[0071] One method of synthesizing Host 4 is to react Cu(OH) ₂ with a solution of pyrazole,

Bu ₄ NOH (1M in water) and Na ₂ HAs0 ₄ -7H ₂ 0 in an organic solvent, such solvents including, without limitation solvents such as methylene chloride, tetrahydrofuran or

dimethylformamide). The solution is then filtered, and the solvent evaporated, to obtain a dark blue powder containing (Bu ₄ N) ₂ [ (As0 ₄ ^3~ ) ₂ ^~ c{cis-Cu"i5^-OH) ₇ ^-pz)i ₈ }].

[0072] In a laboratory scale synthesis, a solution of 968 mg of CuS0 ₄ -5H ₂ 0 dissolved in 50 mL of water and 512 mg KOH (pellets, 85%) dissolved in 50 mL of water were combined and mixed to form a powder of Cu(OH) ₂ . After washing thoroughly with water and then tetrahydrofuran, the copper hydroxide powder was added to a solution of 264 mg pyrazole, 0.415 ml Bu ₄ NOH (1M in H ₂ 0) and 86 mg sodium arsenate (Na ₂ HAs0 ₄ -7H ₂ 0) in 15 ml of tetrahydrofuran, and stirred for 24 hours. The deep blue solution was filtered and the solvent removed.

[0073] The same procedure as that described above can be used for the synthesis of

Host 4 with a phosphate ion encapsulated therein, by substituting tetrabutylammonium phosphate for the Bu ₄ NOH and Na ₂ HAs0 ₄ -7H ₂ 0, resulting in an aggregate containing (Bu ₄ N) ₂ [(P0 ₄ ³ -) ₂ c{ u"3(μ3-OH)(μ-pz)3} ₂ {Cu"3(μ-OH) ₂ (μ-pz) ₄ }3] or (Bu ₄ N) ₂ [ (P0 ₄ ³ Vc{cis- <:υ" ₁₅ (μ-ΟΗ) ₇ (μ-ρζ) ₁₈ }].

[0074] If phosphate and arsenate are both contained within the aqueous solution, they will both be extracted by Host 3 and Host 4, as described herein.

[0075] Another preferred anion-encapsulating aggregate is based on the four-ring

{c/ ^' s-Cu" (μ-ΟΗ)(μ-ρζ)} ₆₊ ι ₂₊ ι ₂₊₆ encapsulating host assembly ("Host 5"). Host 5 can also be denoted as {<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)} ₃₆ , and is one ring set that is representative of

{<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)} ₃₆ . Host 5 shows selectivity for the encapsulation of chloride anions from a solution containing competing ions, where the competing ions would norma lly be favored by the Hofmeister bias. Host 5, like previous hosts discussed herein, results in the wrapping of target ions within a na no-ja r structure, which selectively binds the targeted anions.

[0076] As with the separation methods described above, to selectively separate chloride anions from an aqueous solution, a solvent, the components stoichiometrically (based on the amount of chloride) necessary to create the Host 5 nano-jar, and a counterion contributor (if the base does not act as such) are combined with the aqueous solution containing the target anions to form an organic-aqueous mixture, and the combined components are permitted to react. Upon such reaction, the nano-jars self-assemble around the target anions and transfer into the organic phase, selectively removing chloride ions from the aqueous phase, even in the presence of other anions which would be favored by the Hofmeister bias.

[0077] Alternatively, a solvent, a polymeric chain of [(Ιυ"(μ-ΟΗ)(μ-ρζ)] _∞ and a counterion contributor can be combined with an aqueous solution containing the targeted anion for extraction to form an organic-aqueous mixture, and permitted to react. Upon such reaction, the nano-jars self-assemble around the target anions and transfer in to the organic phase, selectively removing the chloride from the aqueous phase. Separation of the organic phase and aqueous phase solution results in extraction of some or all of the targeted anions from the aqueous solution, even in the presence of other anions which would be favored by the Hofmeister bias.

[0078] To synthesize Host 5, several methods may be employed. First, Cu(OH) ₂ can be prepared by mixing aqueous solutions of CuS0 ₄ -5H ₂ 0 and NaOH. A precipitate of Cu(OH) ₂ forms, which is preferably collected from the solution on a filter, washed with water and then washed with tetrahydrofuran. The Cu(OH) ₂ is then dried and added to a solution of pyrazole and Bu ₄ NCI in THF, forming a solution with a deep blue color. Upon filtration and evaporation, deep blue crystals of the formula (Bu ₄ N)

were obtained by re-crystallization from CH ₂ CI ₂ /Et ₂ 0.

[0079] In a laboratory synthesis, a solution of 968 mg CuS0 ₄ -5H ₂ 0 in 15 ml H ₂ 0 was combined with a solution of 310 mg NaOH in 10 ml H ₂ 0. A blue precipitate formed and was collected on a fritted glass filter. The precipitate was washed 3 times with H ₂ 0 and then with tetrahydrofuran. To the dry Cu(OH) ₂ residue, a solution of 264 mg pyrazole and ~100 mg Bu ₄ NCI in 15 ml THF was added. After stirring overnight, the clear deep blue solution was fi ltered and the solvent removed under vacuum. Deep blue crystals suitable for X-ray diffraction were obtained by recrystallization from CH ₂ CI ₂ /Et ₂ 0.

[0080] Another method of synthesis of Host 5 includes dissolving CuCI ₂ -2H ₂ 0 in

dimethylformamide, and then adding a solution of KOH, pyrazole and Bu ₄ NOH solution (1 M in water) in methanol unde r vigorous stirring. After stirring, the solution ca n be filtered, and the solvent evaporated. The residue can be dissolved in CH ₂ CI ₂ . U pon filtration a nd evaporation of the residue i n CH ₂ CI ₂ solution, a da rk blue powder of the formula (Bu ₄ N) is obtained.

[0081] In a laboratory scale synthesis, 4.262 g CuCI ₂ -2H ₂ 0 was dissolved in 150 ml

dimethylformamide. A solution of 3.255 g KOH 85%, 1.702 g pyrazole and 720 mg Bu ₄ NOH solution (1 M in water) in 30 ml methanol was added in 1-2 ml portions under vigorous stirring. After stirring for one day, the solution was filtered and the solvent was evaporated. The residue was dissolved in CH ₂ CI ₂ , the solution was filtered a nd the solvent was eva porated. 3.914 g da rk blue powder was obtained.

[0082] Additional preferred em bodiments of anion-enca psulating aggregates are based on the th ree-ring 6"), {cis- Cu" ( μ-ΟΗ)(μ-ρζ)} ₈₊ ΐ3+8 encapsulating host assembly ("Host 7"), and {cis- Cu"

(μ-ΟΗ)(μ-ρζ)} ₆₊ ι ₂₊ 9 encapsulati ng host assembly (" Host 8"). Hosts 6 and 7 ca n also be denoted as while Host 8 can be denoted as {cis- Cu"

(μ-ΟΗ)(μ-ρζ)} ₂₇ . Each of Hosts 6 and 7 is a ring set that is representative of

{<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)}29, and Host 8 is a ring set that is representative of {cis- Cu"

(μ-ΟΗ)(μ-ρζ)} ₂₇ . Hosts 6, 7, and 8 show selectivity for the encapsulation of ca rbonate atoms from a solution containing com peting ions where the competing ions would normal ly be favored for extraction into a n organic phase by the Hofmeister bias. Hosts 6, 7 and 8, like the previously described hosts, result in the wrapping of target ions in a nano-ja r structure, which selectively binds the ta rgeted anions.

[0083] As with the sepa ration methods for sulfates described a bove, to selectively

sepa rate carbonate anions from an aqueous solution, a solvent, the components stoichiometrical ly (based on the amount of ca rbonate) necessary to create the Host 6, Host 7 and/or Host 8 " nano-jars," and a counterion contributor (if the base does not act as such) are combined with the aqueous solution containing the target anions to form a n orga nic-aqueous mixture, a nd the combined com ponents a re pe rmitted to react. Upon such reaction, the nano-jars self-assemble a round the target anions and transfer into the orga nic phase, selectively removing ca rbonate anions from the aqueous phase, even in the presence of other a nions which wou ld be favored by the Hofmeister bias.

[0084] Alternatively, a solvent, a polymeric chain of [(Ιυ"(μ-ΟΗ)(μ-ρζ)] _∞ and a counterion contributor are combined with an aq ueous sol ution containing the targeted anion for extraction to form an orga nic-aqueous mixture, a nd are permitted to react. Upon such reaction, the na no-ja rs self-assemble a round the target anions and transfer into the orga nic phase, selectively removing ca rbonate from the aqueous phase. Separation of the organic phase and aqueous solution results in extraction of some or all of the ta rgeted anions from the aqueous solution, even in the presence of other anions which would be favored by the Hofmeister bias.

[0085] To synthesize Hosts 6, 7 and 8, Cu(OH) ₂ ca n be prepared by mixing aqueous

solutions of CuS0 ₄ -5H ₂ 0 and KOH 85%. A precipitate forms, which is preferably washed with water and then THF and dried. The dried solid can then be added to a solution of pyrazole a nd tetrabutylammonium hydroxide in tetrahydrofuran, forming a solution with a deep blue color. Additiona l C0 ₂ , such as in the form of dry ice, can be added to the solution. C0 ₂ from air wil l a lso serve as carbonate source. U pon filtration and

evaporation, a dark blue powder containing (Bu ₄ N) ₂ [C0 ₃ ^2~ c{c s-Cu' -OH)^-pz)} ₂ 9] and (Bu ₄ N) ₂ [C03 ^2" c{c s-Cu"^-OH)^-pz)} ₂₇ ] is obtained.

[0086] I n a la boratory scale synthesis, Cu(OH) ₂ was prepa red by mixing aqueous

solutions of 968 mg CuS0 ₄ -5H ₂ 0 in 20 ml H ₂ 0 with 512 mg KOH 85% in 20 ml H ₂ 0. The precipitate was washed extensively with water, and then with THF. The dried solid was added to a solution of 264 mg pyrazole a nd 270 mg Bu ₄ NOH solution (1 M in water) in 15 ml tetrahydrofura n. A few pieces of dry ice (C0 ₂ ) were added to this sol ution, and it was stirred for one day. The deep blue solution was filtered and the solvent was eva porated. 629 mg da rk bl ue powder was obtained.

[0087] As with the formation of Host 1 and Host 2, all three of the ring combinations described as Hosts 6, 7 and 8 are formed during the synthesis steps described herei n, a nd coexist in the sa me solution. Other synthesis procedures may allow for the preferential formation of one of Hosts 6, 7 or 8. However, as all three a re well suited to carbonate ion extraction, coexistence in the same solution does not negatively impact the selective extraction of carbonate ions.

[0088] Host 2, described above, a lso permits the selective extraction of chromate from aqueous solution, according to the same principles and procedures as described above with relation to sulfate and other target anions. FIG. 6 illustrates the encapsulation of chromate in the chromate-encapsulating assembly.

[0089] With respect to the production of lids for the nano-jars described herein, during formation of the nano-ja rs, smaller alkali or alkali earth metal cations can also be used as the lid-forming counterions for the preparation of M2[C0 ₃ ^2~ c{cis-Cu"^-OH)^-pz)}27] ^2~ , M ₂ , where M is chosen from the group consisting of cesium, rubidium, potassium, sodium, and barium. The cesium, rubidium, potassium, sodium or barium may be added via hydroxides of these components. The products described below were identified by electrospray ionization mass spectrometry in an acetonitrile solution (ESI-:m/z2780, 2984, and 3118).

[0090] For example, in a laboratory synthesis cesium was used to form the lid, as follows:

187 mg Cu(N0 ₃ )2-2.5H ₂ 0 (0.804 mmol), 360 mg CsOH-H ₂ 0 (containing≤10% Cs ₂ C0 ₃ ) and 55 mg pyrazole (0.808 mmol) were added to 10 ml THF, and stirred for 3 days. A dark brown wet residue was removed from the deep blue solution by filtration and the solvent was evaporated to yield 128 mg of a dark blue powder.

[0091] In another laboratory scale synthesis, rubidium was used to form the lid. 187 mg

Cu(N0 ₃ ) ₂ -2.5H ₂ 0 (0.804 mmol), 250 mg RbOH-H ₂ 0 (containing≤15% Rb ₂ C0 ₃ ) and 55 mg pyrazole (0.808 mmol) were added to 10 ml THF, and stirred for 7 days. A dark brown wet residue was removed from the deep blue solution by filtration and the solvent was evaporated to yield 127 mg of a dark blue powder.

[0092] In another laboratory scale synthesis, potassium was used to form the lid. 342 mg

Cu(N0 ₃ ) ₂ -2.5H ₂ 0 (1.47 mmol), 194 mg KOH 85% (2.94 mmol), 100 mg pyrazole (1.47 mmol) and 15 mg K ₂ C0 ₃ (0.109 mmol) were added to 10 ml THF, and stirred for 3 days. A dark brown wet residue was removed from the deep blue solution by filtration and the solvent was evaporated to yield 219 mg of a dark blue powder.

[0093] In another laboratory scale synthesis, sodium was used to form the lid. 187 mg

Cu(N0 ₃ ) ₂ -2.5H ₂ 0 (0.804 mmol), 70 mg NaOH (1.75 mmol), 55 mg pyrazole (0.808 mmol) and 11 mg Na ₂ C0 ₃ -H ₂ 0 (0.0887 mmol) were added to 10 ml THF, and stirred for 4 days. A dark brown wet residue was removed from the deep blue solution by filtration and the solvent was eva porated to yield 82 mg of a dark blue powder.

[0094] In a fina l non-limiting example, ba rium was used to form the lid. I n a laboratory scale synthesis 902 mg Cu(N0 ₃ ) ₂ -2.5H ₂ 0 (3.88 mmol), 1.300 g BaOH-8H ₂ 0 (containing ≤2% BaC0 ₃ ) and 264 mg pyrazole (3.88 m mol) were added to 25 ml THF, and stirred for 7 days. A light blue residue was removed from the deep blue solution by filtration and the solvent was evaporated to yield 629 mg of a da rk blue powder.

[0095] Another process which al lows the formation of a single anion-encapsulati ng

aggregate involves including lead ions with copper in the presence of sulfate during the anion-enca psulating aggregate formation. Addition of lead ions to the copper salt, pyrazole, and base with the aqueous solution containing the a nions to be extracted results in formation of prima rily ("Host 9"), which enca psulates the sulfate present in the solution. The structure of Host 9 was determined by electrospray ionization mass spectrometry, which does not differentiate between possible ring sets.

[0096] For example, in la boratory scale synthesis of Host 9, 15.007 g of CuS0 ₄ -5H ₂ 0 (60.1 mmol), 4.690 g NaOH pel lets (117.3 mmol), 4.092 g pyrazole (60.1 m mol), 19.906 g Pb(N0 ₃ ) ₂ (60.1 m mol) and 3.9 g Bu ₄ NOH (1M in H ₂ 0) (3.9 mmol) we re added to 350 m l THF, and stirred for 7 days. A white solid (PbS0 ₄ , NaN0 ₃ ) was then removed from the deep blue solution by fi ltration and the solvent was evaporated in vacuum to yield 10.292 g of a dark blue powder. The product was purified by recrystallization from toluene solution by diffusion of hexane vapors. The identity of the product was confirmed to be ( Bu ₄ N) ₂ [S0 ₄ ^2~ c{cis-Cu"^-OH)^-pz)} ₃ i] exclusively, by electrospray ionization mass spectrometry in acetonitrile solution (ESI-: m/z 2336).

[0097] One embodiment of anion-encapsulati ng aggregates is based on the formation of ligand-bound pyrazole nano-jars. Long chain alkyi groups, branched alkyi groups or cyclic alkyi groups ca n be attached to pyrazole for incorporation into the encapsulati ng host assemblies for increased solubility in non-polar solvents. Additionally, cha rged ligands can be attached for to pyrazole for incorporation into encapsulating host assemblies to improve solubility in pola r solvents, or to create encapsulating host assem blies with altered recognition, bi nding, or tetheri ng properties, or both. [0098] In particular, one embodiment includes anion-encapsulating aggregates based on encapsulating host assemblies such as {cis-Cu"^-OH)^-4-Mepz)} ₂₇ ("Host 10"), {cis-

OH)^-4-Mepz)} ₃₂ ("Host 15"). These alkyl ligand-bound pyrazole anion-enca psulating aggregates include 4-methylpyrazole ("4-Mepz") a nd are somewhat soluble in pentane, hexane and cyclohexane. Hosts 10, 12, and 14 selectively encapsulate both carbonate and sulfate anions. Hosts 11 and 15 selectively encapsulate sulfate anions. Host 13 selectively encapsulates carbonate anions. These encapsulating host assemblies form anion-encapsulating aggregates which are capable of encapsulating anions present as contaminants in aqueous-hydrocarbon solvent mixtures, including without limitation in- process nuclear waste.

[0099] In a laboratory scale synthesis, the ligand-bound pyrazole containing anion- encapsulating aggregates were formed by adding 304 mg CuS0 ₄ -5H ₂ 0 (1.22 mmol), 94 mg NaOH pellets (2.35 mmol), 100 mg 4-methylpyrazole (1.22 mmol) and 79 mg Bu ₄ NOH (1M in H ₂ 0) (0.079 mmol) to 10 ml THF, and stirring for 13 days. A dark brown-purple solid was removed from the deep blue solution by filtration and the solvent was evaporated to yield 72 mg of a dark blue powder. The identity of the products was confirmed by electrospray ionization mass spectrometry in acetonitrile solution (ESI-: C0 ₃ ¾cis-CuV-OH)^-4-Mepz)} ₂₇ ] ² " _m / _{z 2212>} [so ₄ ¾cis-CuV-OH)^-4-Mepz)} ₂₇ ] ² "

Mepz)} ₂₉ ] ^2" m/z 2374, [S0 ₄ ¾cis-Cu"^-OH)^-4-Mepz)} ₂₉ ] ^2" m/z 2392, [C0 ₃ ¾cis-

[S0 ₄ ¾cis-CuV-OH)^-4-Mepz)} _3i ] ^2" _m / _{z 2554^} [so ₄ ¾cis-Cu'V-OH)^-4-Mepz)} ₃₂ ] ^2~ m/z 2634).

[00100] Another embodiment of an anion-encapsulating host containing a longer chain ligand-bound pyrazole is based on the formation of encapsulating host assemblies including 4-butylpyrazole. Anion-encapsulating aggregates incorporating 4-butylpyrazole are very soluble in pentane, hexane and cyclohexane, and are gradually less soluble in longer hydroca rbons (such as heptane, octane, decalin, and hexadecane). These anion- encapsulating aggregates can be used to encapsulate anions present as contaminants in aqueous-hydrocarbon solvent mixtures, such as those present in in-process nuclea r waste.

[00101] Further, specific liga nd-bound pyrazole-containing anion-encapsulating

aggregates ca n also be formed by com bining a pa rticular metal with copper during the formation of the aggregate.

[00102] When a particula r meta l is added to the copper in preparation of the a nion- enca psulating aggregate, the result may be a homogenous batch of anion-encapsulating aggregates with a pa rticu lar coppe r oxidation state, number of atoms, and targeted enca psulated anion. When a different metal is added to the copper in preparation of the anion-enca psulating aggregate, the result may be a heterogeneous mixture of a nion- encapsulating aggregates with a plu rality of different copper oxidation states, copper atoms, and targeted encapsulated anions.

[00103] For example, where Host 14 is permitted to form anion-encapsulating aggregates in the presence of lead, it enca psulates sulfate ions, to form (Bu ₄ N) ₂ [S0 ₄ ^2~ c{cis-Cu"^- ΟΗ)(μ-4-Μθρζ)} ₃ ι]. I n a laboratory scale synthesis, 304 mg CuS0 ₄ -5H ₂ 0 (1.22 m mol), 94 mg NaOH pel lets (2.35 mmol), 100 mg 4-methylpyrazole (1.22 mmol), 423 mg Pb(N 0 ₃ ) ₂ (1.28 mmol) and 79 mg Bu ₄ NOH (1M in H ₂ 0) (0.079 mmol) were added to 10 m l TH F, a nd stirred for 7 days. A gray-purple solid was removed from the deep blue solution by filtration a nd the solvent was evaporated to yield 96 mg of a da rk blue powder,

(Bu ₄ N) ₂ [S0 ₄ ^2~ c{cis-Cu"^-OH)^-4-Mepz)} _3i ]. The identity of the product was confirmed by electrospray ionization mass spectrometry in acetonitrile solution (ESI-: m/z 2554).

[00104] When other metals a re used in place of lead in the above described reaction, alternative anion-enca psulating aggregates can be formed. For exam ple, when the following metal anions were used with the copper sulfate anion-encapsulating aggregates based on Host 14 which selectively encapsulate sulfates were formed: Pb ²⁺ , Ca ²⁺ , Co ²⁺ , M n ²⁺ , Pr ³⁺ , and Nd ³⁺ . Alternatively, when Zn ²⁺ , Cd ²⁺ , or Ni ²⁺ a re present during the formation of the a nion-enca psu lating aggregate, the result is a mixture of sulfate anion-enca psulating aggregates based on Host 10, Host 11, Host 12, a nd Host 14.

[00105] As with 4-methylpyrazole, described above, when 4-butylpyrazole is added to copper in the presence of a nother metal, the 4-butylpyrazole-based anion-encapsulating aggregates form to selectively encapsu late a desired target anion, to the exclusion of other anions. When the 4-butylpyrazole is added to copper in the presence of another meta l, the result may be a homogenous batch of a nion-enca psulating aggregates with a particu lar copper oxidation state, numbe r of atoms, and targeted encapsulated anion. When a different metal is added to the copper in preparation of the ion encapsulating aggregate, the result may be a heterogeneous mixture of anion-encapsulating aggregates with a plura lity of different coppe r oxidation states, copper atoms, and targeted encapsulated anions.

[00106] A variety of cation counterions can be used to form the lids for the na no-ja rs, including smaller a lka li or alka line-ea rth metal counterions. I n one exa mple, 4- butylpyrazole is added to a solution containing copper in the presence of cesium, to form a carbonate encapsulating aggregate. I n a laboratory sca le synthesis, 187 mg

Cu(N0 ₃ ) ₂ -2.5H ₂ 0 (0.804 mmol), 320 mg CsOH- H ₂ 0 (containing≤10% Cs ₂ C0 ₃ ) and 100 mg 4-butylpyrazole (0.805 mmol) were added to 10 m l THF, and stirred for 4 days. A dark brown wet residue was removed from the deep blue solution by filtration and the solvent was eva porated to yield 171 mg of a dark blue powder. The identity of the product as Cs ₂ [C0 ₃ ^2~ c{cis-Cu"^-OH)^-4-Bupz)} ₂₇ ] _wa s confirmed by electrospray ionization mass spectrometry in tetrahydrofuran solution (ESI-: m/z 2780).

[00107] The use of alternate cou nterions, such as rubidium, potassium, and/or sodium, resulted in heterogeneous mixtures containing varying amounts of anion-encapsulati ng aggregates based on the following encapsulating host assemblies: {α5-(Ιυ"(μ-ΟΗ)(μ-4- Bupz)} ₂₇ , The anion- enca psulating aggregates had the general formulas of M ₂ [C0 ₃ ^2~ c{cis-Cu"^-OH)^-4-

Bupz)} ₃ i], where M = Rb ⁺ , K ⁺ or Na ⁺ . The three products were identified by electrospray ionization mass spectrometry in acetonitrile solution (ESI-: m/z 2780, 2984 and 3188).

[00108] In one la boratory scale synthesis, 187 mg Cu(N0 ₃ ) ₂ -2.5H ₂ 0 (0.804 m mol), 235 mg

RbOH-H ₂ 0 (containing≤15% Rb ₂ C0 ₃ ) and 100 mg 4-butylpyrazole (0.805 mmol) were added to 10 m l THF, and stirred for 7 days. A dark brown wet residue was removed from the deep blue solution by filtration and the solvent was evaporated to yield 137 mg of a dark bl ue powde r, where the da rk blue powder is Rb ₂ [C0 ₃ ^2~ c{cis-Cu"^-OH)^-4-

[00109] In a nother laboratory scale synthesis, 187 mg Cu(N0 ₃ ) ₂ -2.5H ₂ 0 (0.804 mmol), 106 mg KOH 85% (1.61 m mol), 100 mg 4-butylpyrazole (0.805 mmol) and 10 mg K ₂ C0 ₃ (0.0724 m mol) were added to 10 m l THF, a nd stirred for 3 days. A small amount of brown residue was removed from the deep blue solution by filtration and the solvent was evaporated to yield 172 mg of a dark blue powder, where the dark blue powder is K ₂ [C0 ₃ ¾cis-Cu'V-OH)^-4-Bupz)} ₂₇ ], K ₂ [C0 ₃ ¾cis-Cu'V-OH)^-4-Bupz)} ₂₉ ] _{a nd}

K ₂ [C0 ₃ ¾cis-Cu'V-OH)^-4-Bupz)} ₃₁ ].

[00110] In a nother la boratory scale synthesis, 187 mg Cu(N0 ₃ ) ₂ -2.5H ₂ 0 (0.804 mmol), 70 mg NaOH (1.75 m mol), 100 mg 4-butylpyrazole (0.805 mmol) and 12 mg Na ₂ C0 ₃ -H ₂ 0 (0.0968 m mol) were added to 10 m l THF, a nd stirred for 4 days. A da rk brown residue was removed from the deep blue solution by filtration and the solvent was evaporated to yield 164 mg of a dark blue powder, where the da rk blue powder is Na ₂ [C0 ₃ ^2~ c{cis-Cu"^- ΟΗ)(μ-4-Βυρζ)} ₂₇ ], Na ₂ [C0 ₃ ¾cis-Cu'V-OH)^-4-Bupz)} ₂₉ ] and Na ₂ [C0 ₃ ¾cis-Cu'V-

[00111] In another laboratory scale synthesis, 201 mg CuS0 ₄ -5H ₂ 0 (0.80 mmol), 67 mg NaOH (containing≤0.5% Na ₂ C0 ₃ ), 100 mg 4-butylpyrazole (0.80 mmol) a nd 30 mg (0.0523 m mol) bis(triphenylphosphoranylidene) a mmonium chloride ("PPNCI") were added to 10 m l THF, and stirred for 4 days. A dark brown wet residue was removed from the deep blue solution by filtration and the solvent was evaporated to yield 174 mg of a dark bl ue residue. The identity of the products was confirmed by electrospray ionization mass spectrometry in acetonitrile solution (ESI-: m/z 2780 and 2900) to be (PPN) ₂ [C0 ₃ ^2~

[00112] In addition to direct formation of ligand-bound pyrazoles in the formation of a nano-ja r, a variety of ligand bound pyrazoles can be substituted for non-ligand bound pyrazoles that have already been i ncorporated into an anion-encapsulating aggregate. For example, long cha in a lkyl groups, branched alkyl groups, or cyclic alkyl groups that are attached to a pyrazole can be incorporated into anion host assemblies for increased solubi lity i n non-pola r solvents by substitution of the ligand-bound pyrazole for the pyrazole present in the anion host assembly. Additionally, charged ligands bound to pyrazole can be substituted for pyrazole in the anion host assembly, or to improve anion host assembly's solubility in polar solvents, to create anion host assemblies with altered recognition, binding, tethering or other properties (with respect to the original anion host assembly), or both. There are certain ligands which are more easily incorporated into an anion-encapsulating aggregate through substitution than during de novo formation of the anion-encapsulating aggregate. Examples of such ligands include chloride (CI ), bromide (Br ), and iodide (Γ).

[00113] In one embodiment, to perform a ligand exchange reaction, or a substitution, with chloropyrazole on a laboratory scale, 100 mg (Bu ₄ N) ₂ [S0 ₄ ^2~ c{cis-Cu"^-OH)^-pz)} ₃ i] (0.0194 mmol) and 100 mg 4-chloropyrazole (0.975 mmol) were dissolved into 10 ml THF. A small aliquot was immediately diluted with acetonitrile and subjected to analysis by electrospray ionization mass spectrometry. Two minutes after mixing, a mixture of

[(50 ₄ ^2~ )(Ιυ3ΐ(μ-ΟΗ)3ΐ(μ-ρζ) _χ (μ-4-(ΙΙρζ)3ΐ- _χ ] species (x = 5-15) was identified, with [(S0 ₄ ^2~ )(Γυ ₃ ι(μ-ΟΗ)3ΐ(μ-ρζ)ιο(μ-4-ϋρζ)2ΐ] as the most abundant species. Twelve minutes after mixing, x = 2-13, and the most abundant species was [($0 ₄ ^2~ )(Ιυ3ΐ(μ-ΟΗ)3ΐ(μ-ρζ)8(μ-4- Clpz) ₂ 3]. Forty-two minutes after mixing, x = 1-12, and the most abundant species was [(50 ₄ ^2~ )(Ιυ3ΐ(μ-ΟΗ)3ΐ(μ-ρζ)6(μ-4-ϋρζ)25]. The same distribution was found seventeen hours after mixing.

[00114] In another embodiment, to perform a ligand exchange reaction with

bromopyrazole on a laboratory scale, 50 mg (Bu ₄ N) ₂ [S0 ₄ ^2~ c{cis-Cu"^-OH)^-pz)} ₃ i]

(0.00969 mmol) and 87 mg 4-bromopyrazole (0.592 mmol) were dissolved into 10 ml THF. A small aliquot was immediately diluted with acetonitrile and subjected to analysis by electrospray ionization mass spectrometry. Two minutes after mixing, a mixture of [(50 ₄ ^2~ )(Ιυ3ΐ(μ-ΟΗ)3ΐ(μ-ρζ) _χ (μ-4-Βφζ)3ΐ- _χ ] species (x = 3-12) was identified, the species with x = 7 and 8 being the most abundant. Twelve minutes after mixing, x = 2-11, and the most abundant species was [(S0 ₄ ^2~ )Cu3i^-OH)3i^-pz) ₇ ^-4-Brpz) ₂₄ ]. Forty-two minutes after mixing, x = 1-10, and the most abundant species was [($0 ₄ ^2~ )(Ιυ3ΐ(μ-ΟΗ)3ΐ(μ-ρζ)5(μ- 4-Brpz) ₂₆ ]. The same distribution was found seventeen hours after mixing.

[00115] In another embodiment, to perform a ligand exchange reaction with iodopyrazole on a laboratory scale, 50 mg (Bu ₄ N) ₂ [S0 ₄ ^2" c{cis-CuV- ^O (0.00969 mmol) and 118 mg 4-iodopyrazole (0.608 mmol) were dissolved into 5 ml THF. A small aliquot was immediately diluted with acetonitrile and subjected to analysis by e lectrospray ionization mass spectrometry. Two minutes after mixing, a mixture of [($0 ₄ ^2~ )(Ιυ _3ΐ (μ-ΟΗ)3ΐ(μ-ρζ) _χ (μ- 4-l pz) ₃ i- _x ] species (x = 3-11) was identified, with x = 7 being the most abundant species. Twelve minutes after mixing, x = 2-10, and the species with x = 4 and 5 were the most abundant. Forty-two minutes after mixing, x = 1-8, and the most abundant species was with [(S0 ₄ ^2" )Cu ₃ i^-OH) ₃ i^-pz) _x ^-4-lpz) ₂₇ ].

[00116] Similarly, the other target anions described herein, including selenate, selenite, sulfite, pyrophosphate, a nd a rsenite, have large hydration energies and will permit formation of anion-enca psulating host assemblies based on a series of compounds of the formula c/ ^' s-Cu" _x (OR) _y (pz) _z , as described herein, or based on a series of cyclic

polymerization isomers of the formula as described herein.

[00117] Genera lly, as described above, the selective extraction of sulfate, phosphate, arsenate, carbonate, selenate, selenite, sulfite, chromate, phosphate, pyrophosphate and arsenite ions from an aq ueous sol ution can be achieved through the addition of anion- enca psulating host assemblies based on a series of compounds of the form ula c/ ^' s-Cu" _x (OR)y(pz) _z , where R is H, CH ₃ , or another alkyl group, pz is pyrazolate anion and each of x, y, and z is equal to a n integer between 1 and 40, inclusive, or particula rly, a series of cyclic polymerization isomers of the formula [<;/ -(Ιυ"(μ-ΟΗ)(μ-ρζ)]η, where n is an integer between 6 and 16, inclusive. To perform the extraction of these anions from an aqueous solution, a solvent, the components of the encapsulation host assembly (a copper sa lt, a base, (or copper hydroxide i n place of the copper salt and the base) and pyrazole, a nd a suitable counterion contributor (if the base does not act as such), are combined with the aqueous solution conta ining the ta rget ion to form in an organic-aqueous mixture. The components of the organic-aqueous mixture a re permitted to react, and upon such reaction, the anion-encapsulating host assemblies begin forming nano-jars around the ta rget anions in-situ in the orga nic-aqueous mixture. As the nano-ja rs completely form, they transfer to the orga nic phase. The organic and aqueous phases ca n then be separated via traditional separation methods such as a separatory fu nnel.

[00118] Alternatively, rather than adding the i ndividual components of the anion- extracting compou nd, a solvent, a polymer chain of [(Ιυ"(μ-ΟΗ)(μ-ρζ)] _∞ and a suita ble counterion contributor ca n be combined with the aqueous solution containing the target anion to form an organic-aqueous mixture. The components of the organic-aqueous mixture are permitted to react, and upon such reaction, the anion-encapsulating host assemblies begin forming na no-ja rs around the target anions in-situ in the organic- aqueous mixture. As the nano-jars completely form, they transfer to the organic phase. The organic and aqueous phases can then be sepa rated via traditional separation methods such as a separatory funnel.

The above description is considered that of the preferred embodiments only.

Modifications of the inventions will occur to those skilled in the art and to those who make or use the invention. The various devices and methods of providing user information described herein may also be used in combination or separately. Therefore it is understood that the embodiments shown in the drawings and described above are merely for illustrative purposes and not intended to limit the scope of the invention.