Title:
SELECTIVE MARKER FOR TARGET BIOPOLYMER
Document Type and Number:
WIPO Patent Application WO/2011/021581
Kind Code:
A1
Abstract:
A selective fluorescent marker for a biopolymer is provided by developing a fluorescent colorant compound on the basis of a principle that is universally applicable to existing fluorescent colorants having various fluorescent properties, the fluorescent colorant compound being a compound which forms a complex with a biopolymer through a metal ion having no oxidation-reduction reactivity, is capable of selectively luminescing only when associating with the biopolymer, and does not generate nonspecific background fluorescence even without conducting cleaning. The marker comprises a first compound comprising a group of atoms P that sends out a signal and a group of atoms X and a second compound comprising a group of atoms Q that absorbs the signal and a group of atoms Y, wherein the group of atoms X is a specific bonding partner of the group of atoms Y and, when the group of atoms X connects with the group of atoms Y through a noncovalent bond to associate the first compound with the second compound, then the group of atoms Q absorbs the signal sent by the group of atoms P. Also provided is a selective marker for a protein, the marker comprising HisZiFiT as the first compound and BHQ2-His6 or DABCYL-His6 as the second compound or comprising TMR-triNTA as the first compound and DABCYL-His6 as the second compound.
Inventors:
TAKEOKA SHINJI (JP)
ARAI SATOSHI (JP)
MURATA ATSUSHI (JP)
TAKABAYASHI MASAO (JP)
OZAKI MIWAKO (JP)
YOON SU-IN (JP)
ARAI SATOSHI (JP)
MURATA ATSUSHI (JP)
TAKABAYASHI MASAO (JP)
OZAKI MIWAKO (JP)
YOON SU-IN (JP)
Application Number:
PCT/JP2010/063754
Publication Date:
February 24, 2011
Filing Date:
August 13, 2010
Export Citation:
Assignee:
UNIV WASEDA (JP)
TAKEOKA SHINJI (JP)
ARAI SATOSHI (JP)
MURATA ATSUSHI (JP)
TAKABAYASHI MASAO (JP)
OZAKI MIWAKO (JP)
YOON SU-IN (JP)
TAKEOKA SHINJI (JP)
ARAI SATOSHI (JP)
MURATA ATSUSHI (JP)
TAKABAYASHI MASAO (JP)
OZAKI MIWAKO (JP)
YOON SU-IN (JP)
International Classes:
G01N33/542; C07K7/06; C09K11/06; G01N21/78
Domestic Patent References:
| WO2007125822A1 | 2007-11-08 |
Other References:
HAUSER CT ET AL.: "A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure.", PROC NATL ACAD SCI U S A., vol. 104, no. 10, 2007, pages 3693 - 3697
KAMOTO M ET AL.: "Novel probes showing specific fluorescence enhancement on binding to a hexahistidine tag.", CHEM.EUR.J., vol. 14, 2008, pages 8004 - 8012
KAMOTO M ET AL.: "Novel probes showing specific fluorescence enhancement on binding to a hexahistidine tag.", CHEM.EUR.J., vol. 14, 2008, pages 8004 - 8012
Attorney, Agent or Firm:
USHIKI, Mamoru (JP)
牛木 護 (JP)
牛木 護 (JP)
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