Title:
SEPARATION AND PURIFICATION METHOD FOR VANCOMYCIN HYDROCHLORIDE OF HIGH PURITY
Document Type and Number:
WIPO Patent Application WO/2015/062168
Kind Code:
A1
Abstract:
Provided is a separation and purification method for vancomycin hydrochloride of a high purity. The method comprises the following steps: (1) obtaining a vancomycin hydrochloride solution from a crude vancomycin product by ion exchange chromatography and obtaining a concentrate by nanofiltration desalination and concentration; (2) adjusting the concentrate with a hydrochloric acid solution and then performing a column chromatography using a reverse chromatography column for the adjusted concentrate; (3) collecting the chromatographic solution of vancomycin to obtain a mixed chromatographic solution; (4) adjusting the mixed chromatographic solution, and separating the solution and the salts by nanofiltration desalination and concentration to obtain a concentrate; and (5) obtaining a vancomycin dry powder with a chromatographic purity of up to 99% and a pure white appearance by dehydrating and drying the concentrate of step (4), or by solvent crystallization or salting-out crystallization.
Inventors:
LI ENMIN (CN)
ZHUANG YIYUN (CN)
WANG JUE (CN)
SUN XINQIANG (CN)
LAO XUEJUN (CN)
JIANG BIWANG (CN)
ZHUANG YIYUN (CN)
WANG JUE (CN)
SUN XINQIANG (CN)
LAO XUEJUN (CN)
JIANG BIWANG (CN)
Application Number:
PCT/CN2014/000950
Publication Date:
May 07, 2015
Filing Date:
October 27, 2014
Export Citation:
Assignee:
ZHEJIANG MED XINCHANG PHARM (CN)
International Classes:
A61K38/14; C07K1/22; C07K9/00
Domestic Patent References:
WO2006061166A1 | 2006-06-15 | |||
WO2006061166A1 | 2006-06-15 |
Foreign References:
CN101456903A | 2009-06-17 | |||
CN101440127A | 2009-05-27 | |||
CN200710187300A | 2007-11-19 | |||
CN01132048A | 2001-10-30 | |||
CN200710198599A | 2007-12-14 |
Other References:
See also references of EP 3064214A4
Attorney, Agent or Firm:
FAITHFULAW INTERNATIONAL IP LAW OFFICE (CN)
北京乾诚五洲知识产权代理有限责任公司 (CN)
北京乾诚五洲知识产权代理有限责任公司 (CN)
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