Title:
SEQUENCING OF NUCLEIC ACIDS VIA BARCODING IN DISCRETE ENTITIES
Document Type and Number:
WIPO Patent Application WO/2016/126871
Kind Code:
A3
Abstract:
Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.
Inventors:
ABATE ADAM R (US)
HALIBURTON JOHN (US)
LAN FREEMAN (US)
SCIAMBI ADAM R (US)
HALIBURTON JOHN (US)
LAN FREEMAN (US)
SCIAMBI ADAM R (US)
Application Number:
PCT/US2016/016444
Publication Date:
October 06, 2016
Filing Date:
February 03, 2016
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
International Classes:
B01F3/08; C12Q1/68; C40B20/00; C40B50/06; C40B70/00
Domestic Patent References:
| WO2013173394A2 | 2013-11-21 | |||
| WO1994012216A1 | 1994-06-09 | |||
| WO2013134261A1 | 2013-09-12 |
Foreign References:
| US20140378349A1 | 2014-12-25 | |||
| US20140272988A1 | 2014-09-18 | |||
| US20120264646A1 | 2012-10-18 | |||
| US20140323316A1 | 2014-10-30 | |||
| US20140057799A1 | 2014-02-27 |
Other References:
ALI ET AL.: "Rolling circle amplification: a versatile tool for chemical biology, materials science anc medicine", CHEM SOC REV., vol. 43, 18 March 2014 (2014-03-18), pages 3324 - 41, XP055318406
DIETRICH ET AL.: "Effects of UV irradiation and hydrogen peroxide on DNA fragmentation, motility and fertilizing ability of rainbow trout (Oncorhynchus mykiss) spermatozoa", THERIOGENOLOGY., vol. 64, November 2005 (2005-11-01), XP055318415
MIYAZAKI: "Random DNA fragmentation with endonuclease V: application to DNA shuffling", NUCLEIC ACIDS RES., vol. 30, no. e139, 15 December 2002 (2002-12-15), pages 1 - 3, XP055318417
SHUI ET AL.: "Microfluidic DNA fragmentation for on-chip genomic analysis", NANOTECHNOLOGY, vol. 22, no. 494013, 21 November 2011 (2011-11-21), pages 1 - 7, XP020214503
SYED ET AL.: "Next-generation sequencing library preparation: simultaneous fragmentation and tagging using in vitro transposition", NATURE METHODS, vol. 6, 1 November 2009 (2009-11-01), pages 1 - 2, XP055318422
MIYAZAKI ET AL.: "A new large-DNA-fragment delivery system based on integrase activity from an integrative and conjugative element", APPL ENVIRON MICROBIOL., vol. 79, 17 May 2013 (2013-05-17), pages 4440 - 7, XP055108452
HORTON ET AL.: "Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction", BIOTECHNIQUES, vol. 54, 1 March 2013 (2013-03-01), pages 129 - 33, XP009172492
DIETRICH ET AL.: "Effects of UV irradiation and hydrogen peroxide on DNA fragmentation, motility and fertilizing ability of rainbow trout (Oncorhynchus mykiss) spermatozoa", THERIOGENOLOGY., vol. 64, November 2005 (2005-11-01), XP055318415
MIYAZAKI: "Random DNA fragmentation with endonuclease V: application to DNA shuffling", NUCLEIC ACIDS RES., vol. 30, no. e139, 15 December 2002 (2002-12-15), pages 1 - 3, XP055318417
SHUI ET AL.: "Microfluidic DNA fragmentation for on-chip genomic analysis", NANOTECHNOLOGY, vol. 22, no. 494013, 21 November 2011 (2011-11-21), pages 1 - 7, XP020214503
SYED ET AL.: "Next-generation sequencing library preparation: simultaneous fragmentation and tagging using in vitro transposition", NATURE METHODS, vol. 6, 1 November 2009 (2009-11-01), pages 1 - 2, XP055318422
MIYAZAKI ET AL.: "A new large-DNA-fragment delivery system based on integrase activity from an integrative and conjugative element", APPL ENVIRON MICROBIOL., vol. 79, 17 May 2013 (2013-05-17), pages 4440 - 7, XP055108452
HORTON ET AL.: "Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction", BIOTECHNIQUES, vol. 54, 1 March 2013 (2013-03-01), pages 129 - 33, XP009172492
Attorney, Agent or Firm:
RUBIN, Michael B. (Field & Francis LLP1900 University Avenue, Suite 20, East Palo Alto California, US)
Download PDF:
Previous Patent: THERMAL TRANSFER PRINTED POLYMERIC PHONE CASE INSERT
Next Patent: AXIAL RESISTANCE VALVE TRIM DESIGN
Next Patent: AXIAL RESISTANCE VALVE TRIM DESIGN