SET OF POLYPEPTIDES EXHIBITING NUCLEASE ACTIVITY OR NICKASE ACTIVITY WITH DEPENDENCE ON LIGHT OR IN PRESENCE OF DRUG OR SUPPRESSING OR ACTIVATING EXPRESSION OF TARGET GENE

Title:

SET OF POLYPEPTIDES EXHIBITING NUCLEASE ACTIVITY OR NICKASE ACTIVITY WITH DEPENDENCE ON LIGHT OR IN PRESENCE OF DRUG OR SUPPRESSING OR ACTIVATING EXPRESSION OF TARGET GENE

Document Type and Number:

WIPO Patent Application WO/2016/167300

Kind Code:

A1

Abstract:

The present invention provides, for example, a set of two polypeptides that exhibit nuclease activity with dependence on light or in the presence of a drug, wherein an N-terminal fragment and a C-terminal fragment of a Cas9 protein respectively bind to the two polypeptides forming a dimer with dependence on light or in the presence of a drug.

More Like This:

WO/2000/016631	MURF2
WO/1992/016630	DNA SEQUENCES WHICH CODE FOR VIRULENCE CHARACTERISTICS OF STREPTOCOCCUS SUIS AND PARTS THEREOF, POLYPEPTIDES AND ANTIBODIES DERIVED THEREFROM AND THE USE THEREOF FOR THE DIAGNOSIS OF AND PROTECTION AGAINST INFECTION BY S. SUIS IN MAMMALS, INCLUDING MAN
WO/2018/165765	PROBIOTIC MOLECULES FOR REDUCING PATHOGEN VIRULENCE

Inventors:

SATO MORITOSHI (JP)
NIHONGAKI YUTA (JP)

Application Number:

PCT/JP2016/061949

Publication Date:

October 20, 2016

Filing Date:

April 13, 2016

Export Citation:

Click for automatic bibliography generation Help

Assignee:

UNIV TOKYO (JP)

International Classes:

C07K14/315; C12N9/16; C12N15/09; C12N15/113

Domestic Patent References:

WO2014018423A2

2014-01-30

Other References:

ZETSCHE, B. ET AL.: "A split-Cas9 architecture for inducible genome editing and transcription modulation.", NAT. BIOTECHNOL., vol. 33, no. 2, 2 February 2015 (2015-02-02), pages 139 - 142, XP055227889
RAN, F. A. ET AL.: "Double nicking by RNA- guided CRISPR Cas9 for enhanced genome editing specificity.", CELL, vol. 154, no. 6, September 2013 (2013-09-01), pages 1380 - 1389, XP055299681
QI, L. S. ET AL.: "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.", CELL, vol. 152, no. 5, June 2015 (2015-06-01), pages 1173 - 1183, XP055299671
MALI, P. ET AL.: "CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.", NAT. BIOTECHNOL., vol. 31, no. 9, August 2013 (2013-08-01), pages 833 - 838, XP055299678
POLSTEIN, L. R. ET AL.: "A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.", NAT. CHEM. BIOL., vol. 11, no. 3, 2015, pages 198 - 200, XP055194413
NIHONGAKI, Y. ET AL.: "CRISPR-Cas9-based photoactivatable transcription system.", CHEM. BIOL., vol. 22, no. 2, 19 February 2015 (2015-02-19), pages 169 - 174, XP055282700
KAWANO, F. ET AL.: "Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins.", NAT. COMMUN., vol. 6, no. 6256, 24 February 2015 (2015-02-24), pages 1 - 8, XP055321993
NIHONGAKI, Y. ET AL.: "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.", NAT. BIOTECHNOL., vol. 33, no. 7, 2015, pages 755 - 760, XP055321996
HEMPHILL, J.: "Optical Control ot CRISPR/Cas9 Gene Editing.", J. AM. CHEM. SOC., vol. 137, no. 17, 2015, pages 5642 - 5645, XP055282605
"Atarashii Hikari Idengaku: CRISPR-Cas9 System o Oyo shita Kanben Idenshi Hatsugen Seigyoho", EXPERIMENTAL MEDICINE, vol. 33, no. 13, 2015, pages 2139 - 2143, XP009507340
See also references of EP 3284749A4