Title:
SHP2 SPECIFIC INACTIVATING MUTANT PROTEIN AND APPLICATION THEREOF IN CAR-T THERAPY
Document Type and Number:
WIPO Patent Application WO/2021/213479
Kind Code:
A1
Abstract:
A SHP2 specific inactivating mutant protein and an application thereof in CAR-T therapy are provided. Specifically, the SHP2 specific inactivating mutant protein, a corresponding conditionally activated SHP-2 mutant protein expression cassette, and CAR-T cells carrying the conditionally activated SHP-2 mutant protein expression cassette are provided. When the CAR of the CAR-T cells is activated, the accompanying expression of the SHP2 mutant protein can effectively inhibit the endogenous SHP-2 in the CAR-T cells, thereby having a stronger in vitro and in vivo anti-tumor effects.
More Like This:
Inventors:
ZHANG LEI (CN)
GAO MINGMING (CN)
WANG HUIYU (CN)
ZU RUI (CN)
GAO MINGMING (CN)
WANG HUIYU (CN)
ZU RUI (CN)
Application Number:
PCT/CN2021/089087
Publication Date:
October 28, 2021
Filing Date:
April 22, 2021
Export Citation:
Assignee:
FOSUN KITE BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/16; A61K35/12; A61P35/00; C07K14/435; C12N5/10; C12N15/63; C12N15/85
Domestic Patent References:
| WO2011130217A1 | 2011-10-20 |
Foreign References:
| CN107109421A | 2017-08-29 | |||
| US20170143686A1 | 2017-05-25 | |||
| US6156551A | 2000-12-05 | |||
| CN102994621A | 2013-03-27 |
Other References:
WANG XIULI, DONG HONGYAN YANG JINXIA ZHANG MINGSHUANG ZHANG ZHUORAN WU BO XU CHANGQING ZHANG LI: "Protein Tyrosine Phosphatase SHP-2 Inhibits Apoptosis Induced by Serum Withdrawal in 293T Cells", CHINESE JOURNAL OF PATHOPHYSIOLOGY, ZHONGGUO BINGLI SHENGLI XUEHUI, BEIJING, CN, vol. 24, no. 3, 31 December 2008 (2008-12-31), CN , pages 548 - 551, XP055860117, ISSN: 1000-4718
SI YUNFENG, ZHU XIAOCHUN SUN DONGSHENG QI QI WANG GUOYUAN JIN CHENGYAN WANG LINA XU CHANGQING: "Role of SHP-2 in Apoptosis of HEK293T Cells Induced by Arsenic Trioxide", ZHONGGUO YAOLIXUE TONGBAO - CHINESE PHARMACOLOGICAL BULLETIN, LINCHUANG YAOLI YANJIUSUO, HEFEI, CN, vol. 25, no. 8, 31 August 2009 (2009-08-31), CN , pages 1086 - 1089, XP055860112, ISSN: 1001-1978
DONG, HONGYAN; SI, YUN FENG; SUN, DONG SHENG ET AL. : "The Role of SHP-2C459/S Mutant in Apoptosis of HEK293T Cell Induced by Arsenic Trioxide", JOURNAL OF HARBIN MEDICAL UNIVERSITY, vol. 43, no. 4, 31 August 2009 (2009-08-31), China , pages 313 - 315, XP009531381, ISSN: 1000-1905
ZHENG HONG, YU WEN-MEI, WACLAW RONALD R., KONTARIDIS MARIA I., NEEL BENJAMIN G., QU CHENG-KUI: "Gain-of-function mutations in the gene encoding the tyrosine phosphatase SHP2 induce hydrocephalus in a catalytically dependent manner", SCIENCE SIGNALING, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 11, no. 522, 20 March 2018 (2018-03-20), US , XP055860050, ISSN: 1945-0877, DOI: 10.1126/scisignal.aao1591
SI YUNFENG, ZHU XIAOCHUN SUN DONGSHENG QI QI WANG GUOYUAN JIN CHENGYAN WANG LINA XU CHANGQING: "Role of SHP-2 in Apoptosis of HEK293T Cells Induced by Arsenic Trioxide", ZHONGGUO YAOLIXUE TONGBAO - CHINESE PHARMACOLOGICAL BULLETIN, LINCHUANG YAOLI YANJIUSUO, HEFEI, CN, vol. 25, no. 8, 31 August 2009 (2009-08-31), CN , pages 1086 - 1089, XP055860112, ISSN: 1001-1978
DONG, HONGYAN; SI, YUN FENG; SUN, DONG SHENG ET AL. : "The Role of SHP-2C459/S Mutant in Apoptosis of HEK293T Cell Induced by Arsenic Trioxide", JOURNAL OF HARBIN MEDICAL UNIVERSITY, vol. 43, no. 4, 31 August 2009 (2009-08-31), China , pages 313 - 315, XP009531381, ISSN: 1000-1905
ZHENG HONG, YU WEN-MEI, WACLAW RONALD R., KONTARIDIS MARIA I., NEEL BENJAMIN G., QU CHENG-KUI: "Gain-of-function mutations in the gene encoding the tyrosine phosphatase SHP2 induce hydrocephalus in a catalytically dependent manner", SCIENCE SIGNALING, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 11, no. 522, 20 March 2018 (2018-03-20), US , XP055860050, ISSN: 1945-0877, DOI: 10.1126/scisignal.aao1591
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
Download PDF:
Previous Patent: ANTI-HUMAN B7-H3 MONOCLONAL ANTIBODY AND APPLICATION THEREOF
Next Patent: PEDAL SYSTEM FOR ROAD AND FLIGHT OPERATIONAL USE VEHICLE
Next Patent: PEDAL SYSTEM FOR ROAD AND FLIGHT OPERATIONAL USE VEHICLE