Title:
SIDE-WALL HEATER FOR THERMOCYCLER DEVICE
Document Type and Number:
WIPO Patent Application WO2003029397
Kind Code:
A8
Abstract:
The invention relates to an apparatus and methods for preventing condensation on the interior surfaces of sample tubes which are being exposed to temperature cycles, such as during a PCR amplification reaction. In particular, the invention relates to apparatus comprising a side-wall heating block 3 comprising a plurality of sample wells 2 for receiving sample tubes 1 and heating elements disposed in the sample wells for heating at least a portion of the sides of sample tubes e.g., at least the portion which forms the head space after a tube is filled with a PCR reaction mixture. In a preferred aspect, the sample block is part of a thermocycling device for performing PCR and the side-wall heater is used to enhance uniformity and speed of amplification reactions. For, example, by decreasing or eliminating condensation, signal strength jumps in a real-time PCR assay can be minimized as can reaction non-homogeneity.
Inventors:
REID TAYLOR A (US)
TAYLOR ROGER H (US)
BROWN LARRY R (US)
TAYLOR ROGER H (US)
BROWN LARRY R (US)
Application Number:
PCT/US2002/031143
Publication Date:
July 03, 2003
Filing Date:
October 01, 2002
Export Citation:
Assignee:
STRATAGENE INC (US)
REID TAYLOR A (US)
TAYLOR ROGER H (US)
BROWN LARRY R (US)
REID TAYLOR A (US)
TAYLOR ROGER H (US)
BROWN LARRY R (US)
International Classes:
B01L7/00; (IPC1-7): C12M1/38; C12Q1/68
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