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Title:
SIMPLE DETECTION METHOD FOR RNA MODIFICATION, AND METHOD FOR DETECTING TYPE-II DIABETES USING SAID DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2014/136870
Kind Code:
A1
Abstract:
 Provided is a method for detecting modifications present in RNA using small-volume RNA samples. Also provided is a method for detecting modifications in tRNA, such as thio methylation. Also provided is a method of diagnosing human type-II diabetes or the risk thereof, by detecting the thio methylation of tRNA. The present invention is characterized by the generation of cDNA by reverse transcription of RNA using a first primer, and comparing that quantity of cDNA with the quantity of cDNA generated by reverse transcription from RNA using a second primer, thereby detecting modifications (e.g. thio methylation) present in RNA in an RNA sample.

Inventors:
TOMIZAWA KAZUHITO (JP)
WEI FANYAN (JP)
SUZUKI TAKEO (JP)
SUZUKI TSUTOMU (JP)
Application Number:
JP2014/055758
Publication Date:
September 12, 2014
Filing Date:
March 06, 2014
Export Citation:
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Assignee:
UNIV KUMAMOTO NAT UNIV CORP (JP)
International Classes:
C12Q1/68; C12N15/09
Domestic Patent References:
WO2007018169A12007-02-15
WO2008149855A12008-12-11
WO2008109945A12008-09-18
Foreign References:
JP2007295855A2007-11-15
JP2007509613A2007-04-19
Other References:
YANO ET AL.: "Genome-wide exploring A-to-I RNA editing sites in human transcriptome", CLINICAL TESTING, vol. 55, no. 9, 15 September 2011 (2011-09-15), pages 858 - 864, XP008180850
KIRINO ET AL.: "SPECIFIC CORRELATION BETWEEN THE WOBBLE MODIFICATION DEFICIENCY IN MUTANT TRNAS AND THE CLINICAL FEATURES OF A HUMAN MITOCHONDRIAL DISEASE", PNAS, vol. 102, no. 20, 17 May 2005 (2005-05-17), pages 7127 - 7132, XP055280643, DOI: 10.1073/PNAS.0500563102
VENDEIX ET AL.: "HUMAN TRNALYS3UUUIS PRE-STRUCTURED BY NATURAL MODIFICATIONS FOR COGNATE AND WOBBLE CODON BINDING THROUGH KETO-ENOL TAUTOME", J. MOL. BIOL., vol. 416, no. 4, 1 January 2012 (2012-01-01), pages 467 - 485, XP028891688, DOI: 10.1016/J.JMB.2011.12.048
XIE ET AL.: "Simple and Rapid Detection of 2-methylthio Modification in tRNA for Diagnostic Application of Type 2 Diabetes", DAI 90 KAI THE PHYSIOLOGICAL SOCIETY OF JAPAN TAIKAI, 29 March 2013 (2013-03-29), pages S248, XP008180961
See also references of EP 2966178A4
ARRAGAIN S. ET AL., J. BIOL. CHEM., vol. 285, 2010, pages 28425 - 28433
TOMIZAWA ET AL., UEHARA KINEN SEIMEIKAGAKU ZAIDAN KENKYU HOUKOKUSYO, vol. 25, no. 77, 2011
TOMIZAWA ET AL., ENDOCRINE JOURNAL, vol. 58, no. 10, 2011, pages 819 - 825
WEI, F. Y. ET AL., J. CLIN. INVEST., vol. 121, 2011, pages 3598 - 3608
REITER, V. ET AL., NUCLEIC ACIDS RES., vol. 40, 2012, pages 6235 - 6240
SUZUKI, T. ET AL., METHOD. ENZYMOL., vol. 425, 2007, pages 211 - 229
TOMIZAWA ET AL.: "The Uehara Memorial Foundation", RESEARCH REPORT, vol. 25, no. 77, 2011
MIYAUCHI ET AL., NUCLEIC ACIDS RES., vol. 35, 2007, pages E24
WEI, F. Y. ET AL., J. CLIN. INVEST., vol. 121, 2011, pages 3598 - 3608
Attorney, Agent or Firm:
OSAWA, KENICHI (JP)
Ken-ichi Osawa (JP)
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