Title:
SIRNA HAVING MICRORNA-LIKE ACTIVITY FOR PREVENTING OR TREATING COVID-19, AND PHARMACEUTICAL COMPOSITION COMPRISING SAME
Document Type and Number:
WIPO Patent Application WO/2022/203448
Kind Code:
A1
Abstract:
The present invention relates to siRNA having microRNA-like activity for preventing or treating COVID-19, and a pharmaceutical composition comprising same. The siRNA for SARS-CoV-2 according to the present invention was designed by incorporating AGO-CLIP analysis by additionally considering AGO protein accessibility, which was not previously considered in the design of siRNA, to predict the folding structure of the RNA virus and enable the siRNA to accurately effect the target, and targets the conserved region of the SARS-CoV-2. In addition, the siRNA according to the present invention incorporates the seed sequence of miRNA inhibiting pulmonary fibrosis, considered to be a serious problem in SARS-CoV-2, and thus has miRNA-like activity for preventing virus proliferation and inhibiting pulmonary fibrosis. Therefore, the siRNA is expected to be useful as a therapeutic agent that not only inhibits the proliferation of SARS-CoV-2 virus, but can also treat SARS-CoV-2-related diseases.
Inventors:
CHI SUNG WOOK (KR)
GU DO-WOON (KR)
GU DO-WOON (KR)
Application Number:
PCT/KR2022/004219
Publication Date:
September 29, 2022
Filing Date:
March 25, 2022
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12N15/113; A61K31/7105; A61P31/14
Foreign References:
| CN111330003A | 2020-06-26 |
Other References:
GU DOWOON, AHN SEUNG HYUN, EOM SANGKYEONG, LEE HYE-SOOK, HAM JUYOUNG, LEE DONG HA, CHO YOU KYUNG, KOH YONGJUN, IGNATOVA ELIZAVETA,: "AGO-accessible anticancer siRNAs designed with synergistic miRNA-like activity", MOLECULAR THERAPY-NUCLEIC ACIDS, CELL PRESS, US, vol. 23, 1 March 2021 (2021-03-01), US , pages 1172 - 1190, XP055973075, ISSN: 2162-2531, DOI: 10.1016/j.omtn.2021.01.018
MEHTA ADITI, MICHLER THOMAS, MERKEL OLIVIA M.: "siRNA Therapeutics against Respiratory Viral Infections—What Have We Learned for Potential COVID‐19 Therapies?", ADVANCED HEALTHCARE MATERIALS, WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 10, no. 7, 1 April 2021 (2021-04-01), DE , pages 2001650, XP055964903, ISSN: 2192-2640, DOI: 10.1002/adhm.202001650
CHOW JONATHAN TAK-SUM, SALMENA LEONARDO: "Prediction and Analysis of SARS-CoV-2-Targeting MicroRNA in Human Lung Epithelium", GENES, vol. 11, no. 9, 26 August 2020 (2020-08-26), pages 1002, XP055965634, DOI: 10.3390/genes11091002
JAFARINEJAD-FARSANGI SAEIDEH, JAZI MARYAM MOAZZAM, ROSTAMZADEH FARZANEH, HADIZADEH MORTEZA: "High affinity of host human microRNAs to SARS-CoV-2 genome: An in silico analysis", NON-CODING RNA RESEARCH, ELSEVIER, vol. 5, no. 4, 1 December 2020 (2020-12-01), pages 222 - 231, XP055973077, ISSN: 2468-0540, DOI: 10.1016/j.ncrna.2020.11.005
AHN SEUNG HYUN, GU DOWOON, KOH YONGJUN, LEE HYE-SOOK, CHI SUNG WOOK: "AGO CLIP-based imputation of potent siRNA sequences targeting SARS-CoV-2 with antifibrotic miRNA-like activity", SCIENTIFIC REPORTS, vol. 11, no. 1, 1 December 2021 (2021-12-01), XP055973078, DOI: 10.1038/s41598-021-98708-z
MEHTA ADITI, MICHLER THOMAS, MERKEL OLIVIA M.: "siRNA Therapeutics against Respiratory Viral Infections—What Have We Learned for Potential COVID‐19 Therapies?", ADVANCED HEALTHCARE MATERIALS, WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 10, no. 7, 1 April 2021 (2021-04-01), DE , pages 2001650, XP055964903, ISSN: 2192-2640, DOI: 10.1002/adhm.202001650
CHOW JONATHAN TAK-SUM, SALMENA LEONARDO: "Prediction and Analysis of SARS-CoV-2-Targeting MicroRNA in Human Lung Epithelium", GENES, vol. 11, no. 9, 26 August 2020 (2020-08-26), pages 1002, XP055965634, DOI: 10.3390/genes11091002
JAFARINEJAD-FARSANGI SAEIDEH, JAZI MARYAM MOAZZAM, ROSTAMZADEH FARZANEH, HADIZADEH MORTEZA: "High affinity of host human microRNAs to SARS-CoV-2 genome: An in silico analysis", NON-CODING RNA RESEARCH, ELSEVIER, vol. 5, no. 4, 1 December 2020 (2020-12-01), pages 222 - 231, XP055973077, ISSN: 2468-0540, DOI: 10.1016/j.ncrna.2020.11.005
AHN SEUNG HYUN, GU DOWOON, KOH YONGJUN, LEE HYE-SOOK, CHI SUNG WOOK: "AGO CLIP-based imputation of potent siRNA sequences targeting SARS-CoV-2 with antifibrotic miRNA-like activity", SCIENTIFIC REPORTS, vol. 11, no. 1, 1 December 2021 (2021-12-01), XP055973078, DOI: 10.1038/s41598-021-98708-z
Attorney, Agent or Firm:
LEE, Myoung-Jin (KR)
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