Title:
SMALL INTESTINE EPITHELIUM-LIKE CELLS AND PRODUCTION METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/053406
Kind Code:
A1
Abstract:
Research on methods for selective differentiation induction from pluripotent stem cells to small intestine epithelium-like cells is underway. The present invention provides an excellent highly functional small intestine epithelium-like cell population that can be used stably in tests for multidrug metabolism and permeability, and also provides a highly efficient method for producing cells. The present invention involves a method for inducing differentiation from pluripotent stem cells to small intestine epithelium-like cells, the method including following steps 1) and 2). 1) A step of inducing differentiation of pluripotent stem cells into entodermal cells. 2) A step of culturing the entodermal cells in a system comprising CHIR99021 and then inducing differentiation into small intestine epithelium-like cells. A small intestine epithelium-like cell population can be more efficiently obtained by seeding the small intestine epithelium-like cells produced by the differentiation induction method on a base material for producing an organoid or on a base material for two-dimensional culture and culturing.
Inventors:
MIZUGUCHI HIROYUKI (JP)
KAWAI KANAE (JP)
INUI TATSUYA (JP)
KAWAI KANAE (JP)
INUI TATSUYA (JP)
Application Number:
PCT/JP2023/030444
Publication Date:
March 14, 2024
Filing Date:
August 24, 2023
Export Citation:
Assignee:
UNIV OSAKA (JP)
NAT INST BIOMEDICAL INNOVATION HEALTH & NUTRITION (JP)
NAT INST BIOMEDICAL INNOVATION HEALTH & NUTRITION (JP)
International Classes:
C12N5/071; C12N1/00
Domestic Patent References:
| WO2019021990A1 | 2019-01-31 | |||
| WO2020091020A1 | 2020-05-07 |
Foreign References:
| US20220135949A1 | 2022-05-05 | |||
| JP2019506861A | 2019-03-14 | |||
| JP2022019411A | 2022-01-27 | |||
| JP2021122208A | 2021-08-30 |
Other References:
YAMADA SHIGERU, KANDA YASUNARI: "Retinoic acid promotes barrier functions in human iPSC-derived intestinal epithelial monolayers", JOURNAL OF PHARMACOLOGICAL SCIENCES, JAPANESE PHARMACOLOGICAL SOCIETY , TOKYO, JP, vol. 140, no. 4, 1 August 2019 (2019-08-01), JP , pages 337 - 344, XP093148045, ISSN: 1347-8613, DOI: 10.1016/j.jphs.2019.06.012
Attorney, Agent or Firm:
SHOJI, Takashi et al. (JP)
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