Title:
SMALL SPECIMEN STAINING AND DIAGNOSING OF CELLS
Document Type and Number:
WIPO Patent Application WO/2010/081108
Kind Code:
A3
Abstract:
An immunohistochemical staining of small specimen comprises using a plurality of antibodies and/or antigens to mark certain cells with particular colors of stains in order to distinguish target cells, such as carcinoma cells, in a stained small specimen. For example, antibodies CD44, cytokeratin 20 and p53 may be used for selectively staining a specimen of a urothelial mucosal biopsy on a single slide. Mouse monoclonal antibody CD44 is associated only with reactive urothelial cells, while rabbit monoclonal antibody p53 is associated only with carcinoma cells. Mouse monoclonal antibody cytokeratin 20 is associated with both "umbrella cells" and carcinoma cells, but antibody p53 is not associated with umbrella cells, which are the most superficial urothelial cells and are characterized morphologically from the other cells in a prepared specimen. Thus, diagnosis is facilitated by the staining of carcinoma cells in a contrasting color to normal urothelial cells and superficial urothelial cells.
Inventors:
TSIVIS PETER ALLEN (US)
Application Number:
PCT/US2010/020667
Publication Date:
December 02, 2010
Filing Date:
January 11, 2010
Export Citation:
Assignee:
C V DIAGNOSTICS LLC (US)
TSIVIS PETER ALLEN (US)
TSIVIS PETER ALLEN (US)
International Classes:
G01N33/52; G01N1/30; G01N33/53
Foreign References:
| US7452727B2 | 2008-11-18 | |||
| US20060068452A1 | 2006-03-30 | |||
| US5334509A | 1994-08-02 | |||
| US20020090722A1 | 2002-07-11 |
Attorney, Agent or Firm:
PARADIES, Christopher, Ph.D. (501 East Kennedy Blvd. Suite 170, Tampa FL, US)
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