Title:
SNP DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2019/074004
Kind Code:
A1
Abstract:
Provided is a method for detecting SNPs (single nucleotide polymorphisms) using dried blood on filter paper, specifically a method for SNPs (single nucleotide polymorphisms) from nucleic acids in dried blood on filter paper, including (B) a step in which an amplified nucleic acid fragment of a region including SNPs in a nucleic acid in dried blood on filter paper is used as a template, and PCR using SNP identification primers is conducted in a separate reaction system for each SNP identification primer.
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Inventors:
NISHIO HISAHIDE (JP)
SHINOHARA MASAKAZU (JP)
SHINOHARA MASAKAZU (JP)
Application Number:
PCT/JP2018/037732
Publication Date:
April 18, 2019
Filing Date:
October 10, 2018
Export Citation:
Assignee:
UNIV KOBE NAT UNIV CORP (JP)
International Classes:
C12N15/09; C12Q1/686; C12Q1/6883; G01N33/49
Other References:
AR ROCHMAH M. ET AL.: "Genetic screening of spinal muscular atrophy using a real-time modified COP-PCR technique with dried blood-spot DNA", BRAIN DEV., vol. 39, no. 9, 15 May 2017 (2017-05-15), pages 774 - 782, XP085157570, DOI: doi:10.1016/j.braindev.2017.04.015
SHINOHARA, M. ET AL.: "New, Improved Version of the mCOP-PCR Screening System for Detection of Spinal Muscular Atrophy Gene (SMN1) Deletion", KOBE J. MED. SCI., vol. 63, no. 2, 7 September 2017 (2017-09-07), pages E37 - E40, XP055593668
SA'ADAH, N. ET AL.: "A Rapid, Accurate and Simple Screening Method for Spinal Muscular Atrophy: High-Resolution Melting Analysis Using Dried Blood Spots on Filter Paper.", CLIN LAB., vol. 61, no. 5-6, 2015, pages 575 - 580
SHINOHARA, M. ET AL.: "New, Improved Version of the mCOP-PCR Screening System for Detection of Spinal Muscular Atrophy Gene (SMN1) Deletion", KOBE J. MED. SCI., vol. 63, no. 2, 7 September 2017 (2017-09-07), pages E37 - E40, XP055593668
SA'ADAH, N. ET AL.: "A Rapid, Accurate and Simple Screening Method for Spinal Muscular Atrophy: High-Resolution Melting Analysis Using Dried Blood Spots on Filter Paper.", CLIN LAB., vol. 61, no. 5-6, 2015, pages 575 - 580
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
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