Title:
SNP MARKER RELATED TO LOW-SALT TOLERANCE CHARACTERISTIC OF LITOPENAEUS VANNAMEI, AMPLIFICATION PRIMER THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/214187
Kind Code:
A1
Abstract:
Disclosed are an SNP marker related to the low-salt tolerance characteristic of Litopenaeus vannamei, an amplification primer therefor, and an application thereof. A primer is designed on the basis of the mRNA sequence (GenBank: KF765670.1) of the Na,K-ATPase α subunit of Litopenaeus vannamei obtained from NCBI, and the genomic DNA of low-salt tolerant and low-salt intolerant Litopenaeus vannamei are used as templates respectively for PCR amplification to obtain PCR products with a length of 533 bp respectively. The sequencing results are compared and analyzed. A total of 11 SNP sites are detected in this sequence. An SNP marker related to low-salt tolerance is identified by correlative analysis of the genotypes of these templates at each SNP site.
Inventors:
REN CHUNHUA (CN)
JIANG XIAO (CN)
CHEN TING (CN)
HUANG WEN (CN)
HU CHAOQUN (CN)
JIANG XIAO (CN)
CHEN TING (CN)
HUANG WEN (CN)
HU CHAOQUN (CN)
Application Number:
PCT/CN2017/088329
Publication Date:
November 29, 2018
Filing Date:
June 14, 2017
Export Citation:
Assignee:
SOUTH CHINA SEA INST OCEANOLOGY CAS (CN)
International Classes:
C12Q1/68; C12N15/11; C12N15/55
Foreign References:
| CN105969873A | 2016-09-28 |
Other References:
ZHANG LI-TIAN, ET AL: "Effect of K+ and the interaction of major ions on survival, growth and ATP enzymes specific activity of Litopenaeus vannamei", GUANGDONG AGRICULTURAL SCIENCE, vol. 39, no. 6, 30 June 2012 (2012-06-30), pages 116 - 120
Attorney, Agent or Firm:
GUANGZHOU KEYUE I.P. LAW OFFICE (CN)
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