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Title:
SNP SITE CLOSELY ASSOCIATED WITH ANTHOCYANIN CONTENT IN VIGNA UNGUICULATA, KASP MOLECULAR MARKER PRIMER, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/236840
Kind Code:
A1
Abstract:
The present invention relates to the field of molecular genetic breeding, and particularly, to an SNP marker closely associated with anthocyanin content in Vigna unguiculata, a KASP molecular marker primer, a kit, and use thereof. The SNP marker provided by the present invention is located at bp position 2,361,292 of chromosome 5 in Vigna unguiculata genome V1.2, and is an A-to-G substitution. The nucleotide sequence of the wild-type gene of anthocyanin in Vigna unguiculata is set forth in SEQ ID NO 1, in which position 479 comprises the A-to-G mutation.

Inventors:
ZHANG HONGMEI (CN)
ZHANG WEI (CN)
MENG SHAN (CN)
CHEN HUATAO (CN)
LIU XIAOQING (CN)
CHEN XIN (CN)
CUI XIAOYAN (CN)
WANG QIONG (CN)
Application Number:
PCT/CN2023/097679
Publication Date:
December 14, 2023
Filing Date:
June 01, 2023
Export Citation:
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Assignee:
JIANGSU ACAD AGRICULTURAL SCI (CN)
International Classes:
C12N15/11; C12Q1/6895
Foreign References:
CN107475381A2017-12-15
CN109852723A2019-06-07
CN111910013A2020-11-10
Other References:
HERNITER IRA A, MUÑOZ-AMATRIAÍN MARÍA, LO SASSOUM, GUO YI-NING, CLOSE TIMOTHY J: "Identification of Candidate Genes Controlling Black Seed Coat and Pod Tip Color in Cowpea ( Vigna unguiculata [L.] Walp)", G3: GENES, GENOMES, GENETICS, GENETICS SOCIETY OF AMERICA, US, vol. 8, no. 10, 1 October 2018 (2018-10-01), US , pages 3347 - 3355, XP093114523, ISSN: 2160-1836, DOI: 10.1534/g3.118.200521
LONARDI STEFANO, MUÑOZ‐AMATRIAÍN MARÍA, LIANG QIHUA, SHU SHENGQIANG, WANAMAKER STEVE I., LO SASSOUM, TANSKANEN JAAKKO, SCHULMAN AL: "The genome of cowpea ( Vigna unguiculata [L.] Walp.)", THE PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD., GB, vol. 98, no. 5, 1 June 2019 (2019-06-01), GB , pages 767 - 782, XP093114524, ISSN: 0960-7412, DOI: 10.1111/tpj.14349
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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