Title:
SOLID PHASE EXTRACTION MATERIAL AND ITS USE FOR NUCLEIC ACID ENRICHMENT AND DETECTION
Document Type and Number:
WIPO Patent Application WO/2019/011322
Kind Code:
A1
Abstract:
Relating to the technical field of analytical chemistry and biological detection, in particular to a solid phase extraction material and its use in nucleic acid enrichment and detection. A solid phase extraction material modified by rGO or GO. The GO or rGO modified material can quickly and efficiently adsorb nucleic acids, especially short single-stranded nucleic acids, through electrostatic force, hydrogen bond, and π-πforce. The detection limit of nucleic acid samples is accordingly reduced, and the detection sensitivity is accordingly improved.
Inventors:
YAN HE (CN)
XING WANLI (CN)
LU YING (CN)
XING WANLI (CN)
LU YING (CN)
Application Number:
PCT/CN2018/095600
Publication Date:
January 17, 2019
Filing Date:
July 13, 2018
Export Citation:
Assignee:
CAPITALBIO CORP (CN)
UNIV TSINGHUA (CN)
UNIV TSINGHUA (CN)
International Classes:
C12N15/10; C12Q1/68
Domestic Patent References:
WO2016084945A1 | 2016-06-02 |
Foreign References:
CN107236730A | 2017-10-10 | |||
KR101442418B1 | 2014-09-22 | |||
CN105300950A | 2016-02-03 | |||
CN103411933A | 2013-11-27 | |||
CN105713900A | 2016-06-29 |
Other References:
PHAM X. ET AL.: "Graphene oxide conjugated magnetic beads for RNA extraction", CHEM.ASIAN J., vol. 12, no. 15, 22 June 2017 (2017-06-22), pages 1883 - 1888, XP055565002, Retrieved from the Internet
ZHU, X. ET AL.: "Detection of micro RNA SNPs with ultrahigh specificity by using reduced graphene oxide-assisted rolling circle amplification", CHEM. COMMUN., vol. 51, no. 49, 1 May 2015 (2015-05-01), pages 10002 - 10005, XP055565008, Retrieved from the Internet
ZHU, X. ET AL.: "Detection of micro RNA SNPs with ultrahigh specificity by using reduced graphene oxide-assisted rolling circle amplification", CHEM. COMMUN., vol. 51, no. 49, 1 May 2015 (2015-05-01), pages 10002 - 10005, XP055565008, Retrieved from the Internet
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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