Title:
SOLUBILIZED PROTEIN PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2024/057361
Kind Code:
A1
Abstract:
The present invention develops a method involving: solubilizing, without using a modifying agent, an insoluble protein included in an inclusion body, which is aggregate of a recombinant protein expressed in a microorganism; separating same as a solubilized protein without using a column or the like; and purifying same. Provided is a method for producing a solubilized target protein from an inclusion body by mixing an insoluble fraction and a lithium solution, and subsequently separating a soluble fraction including the solubilized target protein from the insoluble fraction.
Inventors:
WATANABE MASANAO (JP)
DOI TAKESHI (JP)
SHIBASAKI MANABU (JP)
DOI TAKESHI (JP)
SHIBASAKI MANABU (JP)
Application Number:
PCT/JP2022/034058
Publication Date:
March 21, 2024
Filing Date:
September 12, 2022
Export Citation:
Assignee:
KOWA CO (JP)
International Classes:
C07K1/14; C12P21/02
Domestic Patent References:
| WO2016153072A1 | 2016-09-29 | |||
| WO2021011431A1 | 2021-01-21 | |||
| WO2021035184A1 | 2021-02-25 | |||
| WO2018030499A1 | 2018-02-15 |
Foreign References:
| JP2014502140A | 2014-01-30 | |||
| JP2012182995A | 2012-09-27 | |||
| US20120214199A1 | 2012-08-23 | |||
| JP2022145193A | 2022-10-03 |
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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