Title:
SORTING OF ADHERENT CELLS BY SELECTIVE TRANSFORMATION OF LABELS
Document Type and Number:
WIPO Patent Application WO/2012/071275
Kind Code:
A3
Abstract:
Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.
Inventors:
ARCHER ROBERT M (US)
HSIUNG FRANK (US)
PATT PAUL (US)
HSIUNG FRANK (US)
PATT PAUL (US)
Application Number:
PCT/US2011/061425
Publication Date:
July 26, 2012
Filing Date:
November 18, 2011
Export Citation:
Assignee:
BIO RAD LABORATORIES (US)
ARCHER ROBERT M (US)
HSIUNG FRANK (US)
PATT PAUL (US)
ARCHER ROBERT M (US)
HSIUNG FRANK (US)
PATT PAUL (US)
International Classes:
C12N5/02; C12N5/00; C12N5/071; G01J1/56; G01J3/30; G01N23/00
Foreign References:
| US20080166378A1 | 2008-07-10 | |||
| US20070238169A1 | 2007-10-11 |
Other References:
WANG Y. ET AL.: "Broadening Cell Selection Criteria with Micropallet Arrays of Adherent Cells", CYTOMETRY PART A., vol. 71A, no. 10, 2007, pages 866 - 874, XP055087020
MAUREL D. ET AL.: "Photoactivatable and photoconvertible fluorescent probes for protein labeling", ACS CHEM BIOL., vol. 5, no. 5, 21 May 2010 (2010-05-21), pages 507 - 516, XP055087028
PATTERSON G.H. ET AL.: "A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells", SCIENCE, vol. 297, no. 5588, 2002, pages 1873 - 1877, XP055087031
GUNN N.M. ET AL.: "Ferromagnetic Micropallets for Magnetic Capture of Single Adherent Cells", LANGMUIR, vol. 26, no. 22, 16 November 2010 (2010-11-16), pages 17703 - 17711, XP055087039
LEE H.-M. ET AL.: "Light-Mediated Spatial Control Via Photolabile Fluorescently Quenched Peptide Cassettes", J AM CHEM SOC., vol. 132, no. 5, February 2010 (2010-02-01), pages 1446, XP055077218
MAUREL D. ET AL.: "Photoactivatable and photoconvertible fluorescent probes for protein labeling", ACS CHEM BIOL., vol. 5, no. 5, 21 May 2010 (2010-05-21), pages 507 - 516, XP055087028
PATTERSON G.H. ET AL.: "A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells", SCIENCE, vol. 297, no. 5588, 2002, pages 1873 - 1877, XP055087031
GUNN N.M. ET AL.: "Ferromagnetic Micropallets for Magnetic Capture of Single Adherent Cells", LANGMUIR, vol. 26, no. 22, 16 November 2010 (2010-11-16), pages 17703 - 17711, XP055087039
LEE H.-M. ET AL.: "Light-Mediated Spatial Control Via Photolabile Fluorescently Quenched Peptide Cassettes", J AM CHEM SOC., vol. 132, no. 5, February 2010 (2010-02-01), pages 1446, XP055077218
Attorney, Agent or Firm:
HEINES, M. Henry et al. (Two Embarcadero Center 8th Floo, San Francisco California, US)
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