Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
SPATIALLY DIRECTED INTERACTION ON A SOLID SURFACE
Document Type and Number:
WIPO Patent Application WO/2000/056444
Kind Code:
A2
Abstract:
A composition of a liquid (26) is caused to interact with a narrow band shaped area at a chosen position on a solid surface within a flow channel (12) by hydrodynamic focusing of a guided stream of said liquid between two streams of guiding liquid (28). By altering the ratio of the flow rates of the two guiding liquid streams, the position of the guided liquid stream is changed and further interaction with the solid surface takes place along a second band shaped area. Using two such flow channels it is possible to produce a two dimensional array of interaction sites.

Inventors:
BLANKENSTEIN GERT (DE)
WEBER ANDERS (DK)
AHL THOMAS (DK)
BONDE MARTIN (DK)
Application Number:
PCT/EP2000/002578
Publication Date:
September 28, 2000
Filing Date:
March 23, 2000
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
TORSANA BIOSENSOR A S (DK)
BLANKENSTEIN GERT (DE)
WEBER ANDERS (DK)
AHL THOMAS (DK)
BONDE MARTIN (DK)
International Classes:
B01D57/02; B01F13/00; G01N37/00; B01J19/00; B03C5/00; C12M1/00; C12N15/09; F15C1/08; G01N15/14; C40B40/06; C40B60/14; (IPC1-7): B01J19/00
Domestic Patent References:
WO1998010267A11998-03-12
WO1999005512A11999-02-04
WO1998049344A11998-11-05
Foreign References:
EP0916396A21999-05-19
Other References:
GERT BLANKENSTEIN ET AL: "MODULAR CONCEPT OF A LABORATORY ON A CHIP FOR CHEMICAL AND BIOCHEMICAL ANALYSIS" BIOSENSORS & BIOELECTRONICS, vol. 13, no. 3/04, 1998, pages 427-438, XP000700154 BARKING, UK ISSN: 0956-5663 cited in the application
GERT BLANKENSTEIN ET AL: "FLOW SWITCH FOR ANALYTE INJECTION AND CELL/PARTICLE SORTING" ANALYTICAL METHODS AND INSTRUMENTATION, 1996, pages 82-84-84, XP000865501 NEW YORK, US ISSN: 1063-5246
Attorney, Agent or Firm:
Smart, Peter J. (Greener & Co. 7 Stone Buildings Lincoln's Inn London WC2A 3SZ, GB)
Download PDF:
Claims:
CLAIMS
1. A method for producing an interaction between a hydrodynamically focused liquid or a component of said hydrodynamically focused liquid and a selected region of a target surface comprising: providing said target surface as part of one of a plurality of surfaces together defining a flow path for liquid flow, the dimension of the surface containing the target surface transverse to the direction of said flow path serving to define the width of the flow path, providing for said flow path a set of at least three fluid inlets and at least one fluid outlet such that a flow of said hydrodynamically focused liquid can be directed into the flow path through one said inlet guided between two flows of guidance liquid introduced via two other said inlets to leave said flow path through said at least one outlet, providing for each pair of guidance liquid inlets flow control means such that the proportion of the total flow of guidance liquid introduced on each side of the said hydrodynamically focused liquid can be varied to position the flow of said hydrodynamically focused liquid laterally within the flow path and at a desired lateral position over the target surface, and directing a flow of said hydrodynamically focused liquid and two flows of guidance liquid through respective ones of said inlets and along said flow path such that the flow of said liquid is directed over a selected region of said target surface having a width less than the width of the target surface and extending at a selected lateral position within said flow path controlled by selection of an appropriate flow ratio of guidance liquid introduced on either side of the flow of said hydrodynamically focused liquid, and allowing said hydrodynamically focused liquid or a component thereof to interact with said selected region of said target surface.
2. A method as claimed in Claim 1, wherein said interaction between said hydrodynamically focused liquid and said target surface involves a chemical reaction.
3. A method as claimed in Claim 2, wherein said chemical reaction is an immunoaffinity reaction, a nucleotide hybridisation reaction, a chemical synthesis reaction, a chemical deprotection reaction, an enzyme catalysed reaction, an enzyme inhibition reaction.
4. A method as claimed in Claim 2, wherein said reaction comprises immobilising a first nucleotide or oligo nucleotide on said surface.
5. A method as claimed in Claim 3, wherein said reaction comprises covalently adding a further nucleotide or oligonucleotide to a nucleotide or oligonucleotide already immobilised on said surface.
6. A method as claimed in Claim 2, wherein said reaction comprises immobilising a first amino acid residue or a peptide on said surface.
7. A method as claimed in Claim 3, wherein said reaction comprises covalently adding a further amino acid or peptide to one already immobilised on said surface.
8. A method as claimed in any preceding claim, wherein the flow of liquid through said flow path is at a Reynolds number of no more than 10.
9. A method as claimed in Claim 8, wherein the flow of liquid through said flow path is at a Reynolds number of no more than 5.
10. A method as claimed in Claim 8, wherein the flow of liquid through said flow path is at a Reynolds number of no more than 1.
11. A method as claimed in any preceding claim, wherein said flow channel is produced by etching of a glass, fused silica or silicon substrate.
12. A method as claimed in any one of Claims 1 to 10, wherein said flow channel is produced by injection moulding of plastics to form a shaped substrate.
13. A method as claimed in any preceding claim, wherein following the production of said interaction at said selected region of said target surface, a second interaction is conducted between a product of said first interaction and a second hydrodynamically focused liquid or component thereof at a selected subregion forming part of said selected region by a method comprising: providing a second plurality of surfaces together defining a second flow path for liquid flow such that one of said surfaces intersects and has a portion in common with said target surface, the dimension of said one surface transverse to the direction of said second flow path defining the width of the second flow path, providing for said second flow path a second set of three fluid inlets and at least one fluid outlet such that a flow of said second hydrodynamically focused liquid can be directed into the flow path through a said inlet guided between two flows of guidance liquid introduced via two other said inlets to leave said flow path through said at least one outlet, providing for each pair of guidance liquid inlets of the second flow path flow control means such that the proportion of the total flow of guidance liquid introduced on each side of the said second hydrodynamically focused liquid can be varied to position the flow of said second hydrodynamically focused liquid laterally within the second flow path, and directing a flow of said second hydrodynamically focused liquid and two flows of guidance liquid through respective ones of said inlets and along said second flow path such that the flow of said liquid is directed over a selected subregion of said common portion of said target surface having a width less than the width of the second flow path and lying at a selected lateral position within said second flow path controlled by selection of an appropriate flow ratio of guidance liquid introduced on either side of the flow of said second hydrodynamically focused liquid, and allowing said second hydrodynamically focused liquid or a component thereof to interact with the product of said first interaction on said selected subregion of said target surface.
14. A method for producing an interaction between hydrodynamically focused liquids or components of said hydrodynamically focused liquids at a selected region of a target surface comprising: providing said target surface at an intersection formed by two crossing flow paths defined by respective sets of flow path bounding surfaces, each said set of bounding surfaces comprising a surface having a width that defines the width of its respective flow path, the target surface being defined by the intersection of said width defining surfaces, providing for each flow path a set of three fluid inlets and at least one fluid outlet such that for each flow path, a flow of hydrodynamically focused liquid can be directed into the flow path through one said inlet guided between two flows of guidance liquid introduced via two other said inlets to leave said flow path via said at least one outlet, providing for each pair of guidance liquid inlets flow control means such that the proportion of the total flow of guidance liquid introduced on each side of the hydrodynamically focused liquid can be varied to position the flow of hydrodynamically focused liquid laterally within the respective flow path, directing a flow of a first hydrodynamically focused liquid along one of said intersecting flow paths to carry out a first interaction between said first hydrodynamically focused liquid or a component thereof and the target surface along a line extending at a selected lateral position within said flow path controlled by selection of an appropriate flow ratio of guidance liquid introduced on either side of the flow of hydrodynamically focused liquid, said interaction producing a product on said target surface, stopping flow through said one flow path, directing a flow of a second hydrodynamically focused liquid along the other one of said intersecting flow paths to carry out a second interaction between said second hydrodynamically focused liquid or a component thereof and the product of said first interaction on said target surface at a point within the intersection of the flow paths lying along a line extending at a selected lateral position within said other flow path controlled by selection of an appropriate flow ratio of guidance liquid introduced on either side of the flow of said second hydrodynamically focused liquid, whereby said second interaction takes place at a selected location within the area of intersection of the two flow paths defined by the selected lateral positions of said hydrodynamically focused liquid flows.
15. A method as claimed in any preceding claim, wherein said flow of hydrodynamically focused liquid and said flows of guidance liquid are electroosmotic flows.
16. Apparatus for use in producing an interaction between hydrodynamically focused liquids or components of said hydrodynamically focused liquids at a selected region of a target surface comprising: a substrate defining said target surface at an intersection formed by two crossing flow paths defined by respective sets of flow path bounding surfaces of the substrate, each said set of bounding surfaces comprising a surface having a width that defines the width of its respective flow path, the target surface being defined by the intersection of said width defining surfaces, a set of at least three fluid inlets and at least one fluid outlet associated with each said flow path such that for each flow path, a flow of hydrodynamically focused liquid can be directed into the flow path through one said inlet guided between two flows of guidance liquid introduced via two other said inlets to leave said flow path via said at least one outlet, and flow control means associated with each pair of guidance liquid inlets such that the proportion of the total flow of guidance liquid introduced on each side of the respective hydrodynamically focused liquid can be varied to position the flow of hydrodynamically focused liquid laterally within the respective flow path.
17. Apparatus as claimed in Claim 16, further comprising a detector for detecting and/or quantitating at selected locations of said target surface products of the interactions of said hydrodynamically focused liquids.
18. A method for producing an interaction between a component of a liquid and a selected region of a target surface, comprising providing a said target surface in contact with a medium through which charged molecules can be caused to migrate, providing oppositely charged driving electrodes at opposed locations of said target surface in electrical contact with said medium to define a migration path between said driving electrodes, providing guiding electrodes of like charge on opposed sides of said migration path in electrical contact with said medium, supplying charged molecules to a starting location in said medium in said migration path and causing said molecules to migrate in said migration path away from one said driving electrode and towards the other said driving electrode whilst laterally electro dynamically focusing said migrating charged molecules to confine their movement within substantially less than the whole width of said migration path by the application of controlled voltages to said guiding electrodes so that the molecules migrate over said selected region of said target surface, and allowing said electrodynamically focused molecules to interact with said target surface.
Description:
INTERNATIONALSEARCH REPORT InternationalApplicationNo PCT/EP00/02578 C.(Continuation)DOCUMENTSCONSIDEREDTOBERELEVANT Category'Citationofdocument,withindication,whereappropriate, oftherelevantpassagesRelevanttoclaimNo. IXGERTBLANKENSTEINETAL:"MODULA 1-4,11 CONCEPTOFALABORATORYONACHIPFOR CHEMICALANDBIOCHEMICALANALYSIS" BIOSENSORS&BIOELECTRONICS, vol.13,no.3/04,1998,pages427-438, XP000700154 BARKING,UK ISSN:0956-5663 citedintheapplication thewholedocument AGERTBLANKENSTEINETAL: "FLOWSWITCH 1 FORANALYTEINJECTIONANDCELL/PARTICLE SORTING" ANALYTICALMETHODSANDINSTRUMENTATION, 1996,pages82-84-84,XP000865501 NEWYORK,US ISSN:1063-5246 thewholedocument AWO9905512A(UNIVERSITYOFWASHINGTON)1 4February1999(1999-02-04) abstract AEP0916396A(AFFYMETRIX,INC.)2-8,13, 19May1999(1999-05-19)14,16 page3,line47-page4,line21 page10,line20-line34 figures7A,7B AWO9849344A(LOCKHEEDMARTINENERGY1-4,11, RESEARCHCORPORATION)13-17 5November1998(1998-11-05) abstract page4,line24-page10,line16 figures1-4 InternationalapplicationNo. INTERNATIONALSEARCHREPORTPCT/EP00/02578 Box IObservationswherecertainclaimswerefoundunsearchable(Continu ationofitem1offirstsheet) ThisInternationalSearchReporthasnotbeenestablishedinrespecto fcertainclaimsunderArticle 17(2)(a)forthefollowingreasons: 1.ClaimsNos.: becausetheyrelatetosubjectmatternotrequiredtobesearchedbythi sAuthority,namely: 2.ClaimsNos.: becausetheyrelatetopartsofthelntemationalApplicationthatdono tcomplywiththeprescribedrequirementstosuch anextentthatnomeaningfulInternationalSearchcanbecarriedout,s pecifically: 3. ClaimsNos.: becausetheyaredependentclaimsandarenotdraftedinaccordancewit hthesecondandthirdsentencesofRule6.4(a). Box11Observationswhereunityofinventionislacking(Continuation ofitem2offirstsheet) ThisInternationalSearchingAuthorityfoundmultipleinventionsin thisinternationalapplication,asfollows: 1.As ai !requiredadditionalsearchfeesweretimelypaidbytheapplicant,th isInternationalSearchReportcoversall searchableclaims. 2.As all searchableclaimscouldbesearchedwithouteffortjustifyinganaddi tionalfee,thisAuthoritydidnotinvitepayment ofanyadditionalfee. 3.As on ! ysomeoftherequiredadditionalsearchfeesweretimelypaidbytheapp licant,thisInternationalSearchReport covers onlythoseclaimsforwhichfeeswerepaid,specificallyclaimsNos.: 4.y No required additionalsearchfeesweretimelypaidbytheapplicant.Consequentl y,thislntemationalSearchReportis restrictedtotheinventionfirstmentionedintheclaims;itiscovere dbyclaimsNos.: 1-17 RemarkonProtestTheadditionalsearchfeeswereaccompaniedbytheap plicant'sprotest. Noprotestaccompaniedthepaymentofadditionalsearchfees.

FURTHER INFORMATION CONTINUED FROM PCT/ISA/210 1. Claims: 1-17 method and apparatus for producing an interaction between a hydrodynamically focused liquid and a selected region of a target surface.

2. Claim: 18 method for producing an interaction between an electrodynamically focused liquid and a selected region of a target surface. INTERNATIONALSEARCHREPORT International ApplicationNo ntormationonpatentfamilymembers pCT/EP 00/02578 PatentdocumentPublicationPatentfamilyPublication citedinsearchreportdatemember(s)date WO9810267A12-03-1998AU4113297 A 26-03-1998 EP0925494 A 30-06-1999 NO991051 A 27-04-1999 WO9905512A04-02-1999US5948684 A 07-09-1999 AU5450498 A 16-02-1999 EP1002227 A 24-05-2000 EP916396A19-05-1999US5384261 A 24-01-1995 US5412087 A 02-05-1995 EP0972564 A 19-01-2000 AU675054 B 23-01-1997 AU3148193 A 15-06-1993 CA2124087 A 27-05-1993 EP0624059 A 17-11-1994 JP7506561 T 20-07-1995 WO9309668 A 27-05-1993 US6040193 A 21-03-2000 US5885837 A 23-03-1999 US5677195 A 14-10-1997 AU4110793 A 29-11-1993 WO9322680 A 11-11-1993 WO9849344A05-11-1998NONE