Title:
SPECIFIC RECOGNITION AND DETECTION METHOD FOR RIBONUCLEIC ACID USING BORONIC ACID MODIFIED NANOPARTICLE SYSTEM
Document Type and Number:
WIPO Patent Application WO/2019/153137
Kind Code:
A1
Abstract:
Disclosed is a specific recognition and detection method for ribonucleic acid using a boronic acid modified nanoparticle system, in the field of nucleic acid detection. Surfaces of gold nanoparticles and silver nanoparticles are modified, by means of thiol self-assembly, with a phenylboronic acid molecule containing a sulfhydryl group, forming a PBA-NP system. The PBA-NP system is capable of efficiently binding to RNA and performing signal labeling and amplification. The greatest advantage of PBA-NPs is their ability to selectively distinguish between RNA and DNA having the same base sequence, and the system can be combined with a variety of nucleic acid detection techniques to achieve specific detection of specified RNA sequences.
Inventors:
PENG WEI (CN)
QIAN SIYU (CN)
QIAN SIYU (CN)
Application Number:
PCT/CN2018/075601
Publication Date:
August 15, 2019
Filing Date:
February 07, 2018
Export Citation:
Assignee:
UNIV DALIAN TECH (CN)
International Classes:
G01N21/64; G01N21/65; G01N27/26
Domestic Patent References:
| WO2011035195A1 | 2011-03-24 | |||
| WO2013039819A2 | 2013-03-21 |
Foreign References:
| CN106596676A | 2017-04-26 |
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