Title:
SPHERICAL MAGNETISABLE POLYVINYL ALCOHOL MICROPARTICLES, METHOD FOR PRODUCING SAME, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2014/015966
Kind Code:
A3
Abstract:
1. The invention relates to spherical magnetisable polyvinyl alcohol microparticles having a particle size distribution in the range from 0.5 to 3 μm, to a method for the production thereof, and use thereof in particular for diagnostic purposes. 2. Spherical magnetisable polyvinyl alcohol microparticles produced according to known methods have a size distribution which is too broad, which is disadvantageous for various reasons. However, particles having a narrow size distribution cannot be produced on a larger scale by known production methods. 3. The method enables the production of spherical magnetisable polyvinyl alcohol microparticles having a particle size distribution in the range from 0.5 to 3 μm. The method comprises the following steps: Dispersing a nanoparticulate magnetisable material in an aqueous phase which contains polyvinyl alcohol in dissolved form; addition of the aqueous phase to an organic phase which is not miscible with the aqueous phase and contains at least one emulsifier; and generating an emulsion by stirring at a temperature of 25 °C or higher; addition of at least one water-soluble cross-linking substance under continued stirring.
Inventors:
OSTER JUERGEN (DE)
SOMMER THOMAS (DE)
A BRASSARD LOTHAR (DE)
SOMMER THOMAS (DE)
A BRASSARD LOTHAR (DE)
Application Number:
PCT/EP2013/002145
Publication Date:
March 13, 2014
Filing Date:
July 18, 2013
Export Citation:
Assignee:
PERKINELMER CHEMAGEN TECHNOLOGIE GMBH (DE)
International Classes:
B03C1/01; C08J3/14; C07K16/26; C08K3/22; C12N15/10; C12Q1/24; G01N33/543; H01F1/44
Domestic Patent References:
| WO1997004862A1 | 1997-02-13 |
Foreign References:
| DE10103652A1 | 2002-08-14 | |||
| US20030109618A1 | 2003-06-12 | |||
| EP1118676A2 | 2001-07-25 |
Other References:
MULLER-SCHULTE D ET AL: "Novel magnetic microspheres on the basis of poly(vinyl alcohol) as affinity medium for quantitative detection of glycated haemoglobin", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, vol. 711, no. 1, 8 September 1995 (1995-09-08), pages 53 - 60, XP004038950, ISSN: 0021-9673, DOI: 10.1016/0021-9673(95)00114-3
CHENGLI YANG ET AL: "Preparation and characterization of monodisperse superparamagnetic poly(vinyl alcohol) beads by reverse spray suspension crosslinking", JOURNAL OF POLYMER SCIENCE PART A: POLYMER CHEMISTRY, vol. 46, no. 1, 1 January 2007 (2007-01-01), pages 203 - 210, XP055095075, ISSN: 0887-624X, DOI: 10.1002/pola.22372
"M-PVA Magnetic Beads", 14 April 2012 (2012-04-14), XP002718375, Retrieved from the Internet [retrieved on 20140107]
JUK RGEN OSTER ET AL: "Polyvinyl-alcohol-based magnetic beads for rapid and e$cient separation of speci"c or unspeci"c nucleic acid sequences", JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 1 January 2001 (2001-01-01), pages 145 - 150, XP055095234, Retrieved from the Internet [retrieved on 20140108]
CHENGLI YANG ET AL: "Preparation and characterization of monodisperse superparamagnetic poly(vinyl alcohol) beads by reverse spray suspension crosslinking", JOURNAL OF POLYMER SCIENCE PART A: POLYMER CHEMISTRY, vol. 46, no. 1, 1 January 2007 (2007-01-01), pages 203 - 210, XP055095075, ISSN: 0887-624X, DOI: 10.1002/pola.22372
"M-PVA Magnetic Beads", 14 April 2012 (2012-04-14), XP002718375, Retrieved from the Internet
JUK RGEN OSTER ET AL: "Polyvinyl-alcohol-based magnetic beads for rapid and e$cient separation of speci"c or unspeci"c nucleic acid sequences", JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 1 January 2001 (2001-01-01), pages 145 - 150, XP055095234, Retrieved from the Internet
Attorney, Agent or Firm:
FLACCUS, Rolf-Dieter et al. (Bussardweg 10, Wesseling, DE)
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