Spider neurotoxiπs and method of producing the same

The present invention relates to a novel toxin, and a method of producing a toxin, particularly but not exclusively to an insect specific neurotoxin S -Latroinsectotoxin («f-LIT), and a method of producing same .

A family of high molecular weight neurotoxiπs has been found in the venom of the black widow spider (Latrodectus mactans Tredecimguttatus ) . Some of these toxins have been identified as being either vertebrate or invertebrate specific. «r-Latrotoxin (<χ-LT) and or -Latroinsectotoxins (©< -LIT) are two such neurotoxins that have been characterised as being vertebrate and invertebrate specific respectively. The primary structures of these proteins have been determined, but characterisation of the structural features of the cloned toxins has not been possible due to the inability to achieve functional expression of their genes.

It is an object of the present invention to provide a novel toxin and a method of producing a toxin usually naturally produced by post-translational modification o f a precursor protein, using reco binant technology.

According to the present invention there is provided a polypeptidε, such as a toxin, formed by expression of a truncated form of a gene sequence, or an

analogue thereof.

Preferably the polypeptide is a neurotoxin and preferably corresponds to a toxic derivative of a substantially non-toxic precursor polypeptide encoded by the gene sequence. The polypeptide may comprise an amino acid sequence that corresponds to a truncated form of the amino acid sequence of a substantially non-toxic precursor polypeptide. Preferably the amino acid sequence of the polypeptide corresponds to the amino acid sequence of the precursor polypeptide with truncation thereof principally at the carboxy (C) end, and desirably by about 150 to 200 amino acids. The polypeptide amino acid sequence may in addition correspond to the precursor polypeptide amino acid sequence truncated at the amino end (N) preferably by less than 50 amino acids, and desirably by 7 or 28 amino acids.

The amino acid sequence of the polypeptide may be homologous to the amino acid sequence of the insect specific neurotoxin eC-Latroιnsectotoxιn ( _< f-L.IT) or an active derivative thereof, and preferably comprises an amino acid sequence as shown in SEQIDN01 and SEQIDN02 or an active derivative thereof. Preferably the toxin is expressed from a nucleotide construct or truncated form of the gene sequence comprising a sequence as shown in SEQIDN01 , or active variants thereof. Preferably the

toxin is expressed from a sequence substantially as provided in a microorganism deposited at The National Collections of Industrial and Marine Bacteria Limited, under Accession No. NCIMB 40632.

The invention also provides a protein for use as a toxin comprising an amino acid sequence substantially as shown in SEQIDN01 and SEQIDN02, or an active derivative thereof.

According to a further aspect of the present invention there is provided a nucleotide sequence comprising a truncated form of a gene sequence or an analogue thereof, for use in the expression of a polypeptide, such as a toxin.

Preferably the nucleotide sequence corresponds to a gene encoding for a precursor polypeptide and truncated at the 3' end thereof or an active derivative thereof. Preferably the nucleotide sequence corresponds to the gene truncated by about 400 to 650 nucleotide bases, and desirably between 550 to 600 nucleotide bases.

The nucleotide sequence may also correspond to the gene truncated at the 5' thereof, preferably by less than 100 nucleotide bases, and desirably by either 84 or 21 nucleotide bases .

Preferably the nucleotide sequence corresponds to part of a gene encoding for a neurotoxin in the venom of the Black Widow Spider (Latrodectus mactans Tredeci guttatus ) , or an active derivative thereof.

The nucleotide sequence may correspond to part of the gene encoding the precursor polypeptide of insect specific toxin cT-Lactoinsectotoxin ( cf-LIT), or an active derivative thereof. The nucleotide sequence preferably codes for a polypeptide comprising a sequence of 991 amino acids.

Preferably the nucleotide sequence comprises a base sequence as shown in SEQIDN01, or an active derivative thereof, and preferably as comprised in a microorganism deposited under Accession No. NCIMB 40632 at The National Collections of Industrial and Marine Bacteria Limited .

Preferably the nucleotide sequence codes for a polypeptide having an amino acid sequence as shown in SEQIDN01 and SEQIDN02, or an active derivative thereof.

The nucleotide sequence may be a cDNA derived from mRNA by the use of an enzyme such as reverse transcriptase . The nucleotide sequence may alternatively be an oligonucleotide DNA construct produced perhaps using the polymerase chain reaction (PCR).

According to a further aspect of the present invention there is provided a method of producing a polypeptide, the method comprising producing a recombinant DNA molecule comprising a truncated form of a gene, and expressing the truncated form in a host expression system, such as a viral or bacterial expression system, to produce the polypeptide.

Preferably the polypeptide produced is an active toxin and desirably a neurotoxin substantially as defined above. Preferably the truncated form comprises part of a gene which encodes for a non-toxic precursor polypeptide.

Preferably the truncated form comprises a nucleotide sequence substantially as defined above, and as shown in SEQIDN01 , or an active derivative thereof. Preferably the expression system comprises E . coll BL21 (DE3) bacterial cells transformed with pT7-7 vectors comprising the truncated form of the sequence, desirably substantially as deposited under Accession No. NCIMB 40632 at The National Collections of Industrial and Marine Bacteria Limited. The expression system may comprise a baculovirus system.

In a further aspect of the present invention there is provided a recombinant DNA molecule, such as a virus, and in particular a baculovirus comprising a truncated form of a gene encoding for a toxin generally

as defined above, and substantially as provided in the microorganism deposited under Accession No. NCIMB 40632.

A still further aspect of the present invention provides an expression vector comprising a truncated form of a gene, the truncated form encoding for a toxin generally as defined above.

The invention also provides a cell, such as a viral or bacterial cell transformed with a recombinant molecule as defined above.

There is also provided an insecticide comprising a toxin as defined above. The insecticide may be so as to be administered orally or topically. The insecticide may comprise a spray .

This invention also provides an insecticide system comprising means for expressing a truncated form of a gene to produce a toxin as described above in an insect to kill or incapacitate the insect. The insecticide system may comprise a viral expression system, and desirably a baculovirus expression system.

According to a further aspect there is provided a plant comprising a genetically modified cell containing a truncated form of a gene sequence substantially as defined above.

Still further according to the present invention

there is provided a non-human animal comprising a genetically modified cell containing a truncated form of a gene sequence substantially as defined above.

According to a further aspect of the present invention there is provided a toxin formed by processing of a substantially isolated non-toxic precursor polypeptide .

The toxin is preferably a neurotoxin and is preferably formed by truncation toward the carboxy (C) end of the precursor polypeptide, preferably by site-directed mutagenesis. Desirably the toxin amino acid sequence generally corresponds to the amino acid sequence of the precursor polypeptide, truncated by between 150 and 200 amino acids. The toxin amino acid sequence may also be formed by truncation toward the amino (N) end of the precursor polypeptide amino acid sequence, the fragment cleaved therefrom preferably being significantly smaller than the fragment cleaved from the carboxy end, and may comprise 7 or 28 amino acids.

Preferably the toxin has an amino acid sequence corresponding to polypeptide encoded by part of a gene of the Black Widow Spider (Latrodectus mactans Tredecimguttatus ) . The toxin may comprise or be an analogue of the insect specific neurotoxin -Latroinsectotoxin ( -LIT) , or an active derivative

t he r e o f .

Preferably the toxin comprises an amino acid sequence as shown in SEQIDN01 and SEQIDN02 or an active derivative thereof.

In a further aspect of the present invention there is provided a method of producing an active polypeptide from an inactive precursor polypeptide, the method comprising truncating the isolated precursor polypeptide.

Preferably the isolated precursor polypeptide is truncated at the Carboxyl end, perhaps using proteolytic cleavage, and preferably by site directed mutagenesis. Truncation of the N terminus may also be provided. Preferably the active polypeptide is a toxin and is substantially as described above.

According to another aspect of the present invention there is provided an isolated nucleotide base sequence encoding for a toxin precursor polypeptide as defined above and preferably with an amino acid sequence as shown in SEQIDN04 or an active derivative thereof. The base sequence preferably comprises the sequence shown in SEQIDN03 or a derivative thereof. The nucleotide base sequence preferably encodes a precursor polypeptide of the neurotoxin -Latroinsectotoxin ( ^ ^" -LIT) . Preferably the base sequence is substantially

as provided in the microorganism deposited under Accession No. NCIMB 40633.

In a further aspect there is provided a recombinant DNA molecule such as a virus, and more particularly a baculovirus comprising a sequence as defined in the preceding paragraph.

In a still further aspect the invention provides a cell, such as a bacterial cell or viral cell, transformed with a recombinant DNA molecule as described in the preceding paragraph.

This invention also provides an insecticide system comprising means for expressing a gene as described above to produce a precursor polypeptide as described above and to process the precursor polypeptide to produce a toxin in an insect to kill or incapacitate the insect. The insecticide system may comprise a viral expression system, and desirably a baculovirus expression system.

According to a further aspect there is provided a plant comprising a genetically modified cell containing a gene as defined above.

Still further according to the present invention there is provided a non-human animal comprising a gene¬ tically modified cell containing a gene as defined above.

Preferred embodiments of the present invention will ow be described by way of example only, with reference to the accompanying sequences, in which:-

SEQ ID NO. 1 shows the nucleotide base sequence and the corresponding amino acid sequence of a truncated form of a gene and a polypeptide encoded thereby, according to one aspect of the present invention;

SEQ ID NO. 2 shows the polypeptide sequence of SEQIDN01 ;

SEQ ID NO. 3 shows the nucleotide base sequence and the corresponding amino acid sequence of a gene and a polypeptide encoded thereby, according to another aspect of the present invention; and

SEQ ID NO. 4 shows the polypeptide sequence of SEQIDN03.

Referring to the sequences, a polypeptide such as a toxin as in SEQIDN02 is formed by expression of a truncated form of a gene sequence (SEQIDN01), or an analogue thereof.

A toxin from Black Widow Spider (Latrodectus actans Tredeci gut atus ) venom (BWSV),

c-f-Latroinsectotoxin , (J ^~ -LIT) has been purified and shown to possess insect specific toxicity. The cf-LIT structural gene has been cloned and sequenced and the N- and C termimi of the native (precursor) and functional protein toxin have been determined as described below. Site directed mutagenesis of T-LIT cDNA enabled expression of the mature protein product (toxin) in bacteria, and this has been shown to be toxic to locusts.

Expression and production of this and other such toxins in bacterial expression systems has hitherto not been possible. The invention includes identification of the sites for cleavage of the precursor protein to produce the toxin, and the precise site of truncation of the gene sequence which has enabled the toxin to be expressed in bacterial, and indeed other suitable hosts.

Microorganism deposits have been made under the Budapest Treaty on 3rd May 1994, at the National Collections of Industrial and Marine Bacteria Limited, of 23 St. Machar Drive, Aberdeen, Scotland, United Kingdom. Escherichia coll ( XL-1 Blue pT7.<fM) cloned with the truncated form of the gene sequence is deposited under Accession No. 40632, and Escherichia coli (HMS 174 pT7. FL) cloned with substantially the full gene sequence is deposited under Accession No. 40633).

In more detail, the cDNA cloning and sequencing was

conducted as follows. Poly(A+)-RNA was isolated from venom glands of the Black Widow Spider (Latrodectus mactans Tredecimguttatus) and a cDNA library constructed in the plasmid vector pSP65 (according to Kiyatkin et al,

4 1993) . A library of 6x10 clones was screened with an end-labelled 23-mer oligonucleotide probe based on the

N-terminal sequence of ,s-v -LIT (amino acid residues 1-8)-

5' GA(C/T)GA(A/G)GA(A/G)GA(C/T)GG(A/T)GAAATGAC 3' .

Hybridization was performed. Positive clones were colony-pun fled and analysed by restriction mapping. The inserts were excised and fragmented by sonication as described (Sambrook et al, 1989) followed by cloning into the Smal site of pBluescπpt II SK+ and SK- vectors

(Stratagene, USA) . Single-stranded templates for sequencing were obtained after infection with helper phage VCS (Stratagene). The DNA sequences were determined by the chain-ter ination method (Sanger et al,

1977) using Sequenase 2.0 version kit (USB Corporation) and T7 and T3 vector-specific primers (Stratagene). Each sequence was determined at least twice on both strands.

Synthetic primers were used to sequence regions that were not covered by isolated subcloned fragments.

DNA and protein sequence analysis was performed using the computer software DNASTAR (Dnastar Inc) and PCGENE ( IntelliGenetics Inc) . This work benefitted from the GCG programme mounted on the SERC Daresbury SEQNET

facility (Devereux, Haeberli and Smithies, (1984), Nucleic Acids Research 12(1); 387-395).

The full-length cDNA construction was carried out as follows. Two sets of oligonucleotide primers were used to produce N- and C- overlapping parts of tS -L U coding sequences by polymerase chain reaction. To facilitate subcloning into the expression vector the 5' sense primer (P1) ( TTGGGATCCGATGAAGAAGATGGAGAA) and 3' antisense primer (P8) (CAATGGTCGACACAGAAGGAATGGTA) contained BamHI and Sail restriction enzyme sites. Two other primers -P9, sense (GTCTGAACCATTTACTGTCC) (position 1283-1302) and P3, antisense (GTAAGATTACCATCTGCAAC ) (complementary to position 2253-2272) were chosen to produce overlapping fragments with an internal Ncol (2056) restriction site. An oligonucleotide was designed to terminate the protein sequence after amino acid 991- 5* CGTTTCGTCGACTCATTCCGGTAAAGTACGACGAAA 3' . The polymerase chain reaction was performed using 1 unit of Taq-polymerase (Promega) under standard conditions (30 cycles, 55°C for 1 min, 72°C for 3 min, 94°C for 1 min, with 100 pmol of each primer and 1-10 ng first-strand cDNA). In the first cycle the deπaturation time was elongated to 5 min. The molecular mass of the amplified material was checked on an agarose gel. First-strand cDNA synthesis was carried out using First-strand cDNA Synthesis Kit (Pharmacia) with both random and specific

primers as recommended by the manufacturer. The PCR products were purified from agarose gel using GeneCleaπ Kit (Bio 101 Inc. ) , digested with appropriate pairs of enzymes (BamHI and Ndel for the N-terminus part and Sall/Ndel for the C-terminus) and cloned into the pT7-7 vector restricted with the similar pairs of enzymes. The full-length cDNA was created as a result of three-way ligation between N-terminal BamH I/Ndel-fragment , C-terminal Ndel/Sal I-fragment and pT7-7 BamHI/Sall- digested vector. The final construct had eight addi¬ tional amino acid residues at the amino terminal end (MARIRARG). All plasmid constructs were verified by sequencing from both ends and through the junction region. The full length construct was designated pT7&.FL a sample of which is deposited at the NCIMB, accession No 40633 and the truncated clone (1-991 amino acids) was designated pT7.£M. (NCIMB No 40632).

In order to verify the identity of the -LIT cDNA, this clone was expressed in the bacterial pT7-7 vector in E.coli BL2KDE3) cells. A full-length toxin cDNA (corresponding to Asp residue 29 to 1186) 1214 of SEQI0N04 was constructed and designated pT7. _< s^FL. The first 28 amino acids are believed to be present in the precursor polypeptide in spider venom glands, but cleaved during N-terminal processing. The recombinant protein constitutes approximately 10 % of the total bacterial

lysate protein. A polyclonal antibody specific was raised to <d-LIT purified from spider venom glands, and demonstrated to be specific for the J ^" toxin. This protein specifically detected a protein of 130 kDa in bacteria expressing recombinant full-length *_T-LIT. Comparison of the molecular mass of the bacterially expressed full-length ^-LIT and the toxin purified from venom glands demonstrated a size difference of approximately 23kDa, in agreement with the calculated molecular mass. The full-length <? -LIT had no toxicity towards insects and is considered to be an inactive precursor form of the toxin.

c- -LIT purified from venom glands was analysed by mass spectrometry (on a Kratos Kompact MALDI 3 Mass Spectrometer, using sinapinic acid as a matrix. The nitrogen laser excitation was at 337nm, and the positive ion was detected in the linear mode) yielding a prominent molecular ion with a m/z+ ratio of 110916. This corresponds closely to the expected molecular mass of ^ -LIT which is truncated at amino acid 991. By comparison, the bacterially expressed full length £ -LIT yielded a molecular ion with an m/z+ ratio of 133631 (VK, DRB, PNRU, Data not shown), within 100 Da of the calculated value. Site directed mutagenesis was used to create a novel _< _- -L I T cDNA clone (pT7 _< ^" M), which was truncated after amino acid 991 of the ^ -LIT sequence

(SEQIDN02). This protein was expressed in bacteria, yielding a protein of similar molecular mass to the mature toxin isolated from spider venom.

E. coli BL21(DE3) cells transformed with pT7 clones were grown in LB medium containing 100mg ampicillin/ml at

30°C to an A, _nn of approximately 0.5. Then 600nm ^ ^r ' expression was induced by addition of IPTG (1mM) to the medium, and incubation continued for 1 hour. For functional studies, bacteria were washed and resuspended in 50 mM TrisHCI, 100mM NaCl, 10mM KC1, 0.4?ό Triton X-100, 12?ό (W/V) sucrose, 5mM DTT, 2 tg/ml aprotonin, 2mM EDTA, pH8, and sonicated on ice. Ammonium sulphate was added to the cleared supernatant to a final concentration of 20 % of saturation, and the pellet was resuspended in buffer without DTT. These samples (5-15/tl) were used for thoracic injection into locusts (100-300 mg body weight); each test was performed on more than 4 locusts, and the locusts were examined for toxicity for 24 hours. Extracts from pT7-7 and pT7.&FL produced no effects on the locusts, but extracts from bacteria carrying pT7.όM caused rapid lethality. The time of death of the locusts varied from 5 minutes - 4 hours, depending on the potency of the batch of toxin.

Preliminary studies were undertaken on

neurally-excited and resting retractor unguis nerve-muscle preparations isolated from metathoracic legs of adult (male and female) locusts (Usherwood and Machili, 1968). cf-LIT was applied in standard locust saline (mM: NaCl, 180; KC1, 10; CaC12, 2; Hepes, 10 (pH 6.8)). A few studies were undertaken using saline in which CaC12 was omitted. Mechanical responses were recorded using a Grass strain guage connected to a Grass pen recorder. Recordings of miniature excitatory postsynaptic potentials were made from fibres of metathoracic extensor tibiae muscles of adult locusts (either sex) using intracellular microelectrodes (approximately 1 Om 1 resistance ) . cf-LIT was applied in either standard locust saline, saline in which CaC12 was omitted or saline which contained MgC12 substituted for NaCl. The miniature potentials were recorded on video tape and analysed on a MassComp computer using m-house software. Membrane bilayers were formed at the tips of patch pipettes (diam 1-2 <m fabricated from Clark Electromedical glass) from onolayers of either diphytanoyl phosphatidylcholine or a mixture of 9 parts isolectin and 1 part cholesterol using a pipette dipping technique (Moπtal and Muller, 198). Similar patch pipettes were used to excise membrane patches from locust metathoracic extensor tibiae muscle fibres (Huddle et al). In order to reduce the activities of endogenous potassium channels KC1 was eliminated from the pipette

and bath salines.

The neurally-evoked twitch contraction of the locust retractor unguis muscle was reduced by

~ 11 approximately 40 % by 10~ M _< -LIT (applied in standard saline) and was abolished during application of

-10 10 M toxin. Small spontaneous contractions sometimes occurred during e -LIT application. The changes in twitch amplitude were accompanied by an irreversible muscle contracture. The appearance of the contracture was delayed and its a plitude was reduced when the concentration of £ -LIT was lowered. A muscle contracture also occured when toxin was applied when the muscle was not neurally stimulated. Twitch contractions

-10 do not occur in calcium-free saline and when 10 M toxin was applied to a preparation equilibrated in this saline a contracture did not occur even after 30 min application of the toxin.

When inside-out patches excised from locust muscle

-11 fibres were exposed to 10 M <= -LIT in the patch pipette, channel opening, of maximum conductance approximately 40pS, were observed. Channel openings of this type were never seen in the absence of toxin.

The channel current exhibited inward rectification when the patch pipette and bath contained identical salines

(including 2mM CaC12) , and channel open times were longer at negative than at positive pipette potentials. hen

there was a 10-fold Ca2+ gradient across a patch, the reversal potential of the channel current was +/- 15mV, the sign being dependent on the Ca2+ gradient.

In the artificial bilayer studies where 10 -11 M

_Q T -LIT was placed in the patch pipettes, single channel openings of approximately 30pS conductance were observed. These channels were not seen when toxin was omitted from the patch pipette. With identical salines

(containing 2mM CaC12) in the patch pipette and bath, the current-voltage characteristic of the^-LITx channel was sigmoidal with a reversal potential at OmV. The channel was shown to be Ca-selective by manipulating the ionic regimes of patch pipette and bath.

A cDNA library from venom gland cDNA was screened with a 23-bp oligonucleotide probe corresponding to the N-terminal sequence of ©T -LIT (as described above). To reduce the number of nucleotide ambiguities the codon usage data available from the nucleotide sequences of e -LT and o<-LIT cDNA (Kiyatkin et al, 1990, Kiyatkin et al 1993) was referred to. Five positive cDNA clones were colony-purified and sequenced. The longest clone (pDT-1) contained more than 2 (kb) of oT-LIT coding region. A PstI-3' fragment was used to rescreen the cDNA library to search for clones encoding the C-terminal part of the toxin. An additional cDNA clone, pDT-17, was isolated,

which covered the C-terminal coding region of the & -LIT. Two overlapping clones, covering the entire open reading frame, have been sequenced in their entirety. The two clones have been demonstrated to be part of a single, continuous RNA from venom glands by polymerase chain reaction across the overlapping region, using two distinct sets of primers. The composite clones encode a cDNA with a frame of 3642 bp starting from the first in-frame Methionine and ending with TAA stop codon (SEQIDN03) .

The Met residue is preceded by an in-frame stop codon confirming the full length of the deduced sequence.

cf -LIT was purified to homogeneity from Black Widow Spider venom by three rounds of column chromatography according to (Krasnoperov et al, 1992) . 23 amino acid residues of the N-terminal sequence of J -LIT was sequenced. The pure toxin was digested with trypsin and seven individual peptides were isolated and partially sequenced .

Direct N-terminal sequence determination demonstrates that the mature protein starts from the sequence DEEDGEM... , so residue 1 in SEQIDN01 and 2 is the first Asp of this sequence. The deduced polypeptide starting from Asp (+1 ) consists of 1186 amino acid (as

shown in SEQIDN03 , Asp residue 29 to residue 1214) residues with a predicted molecular mass of 132671 Daltons and pi of 5.4. It contains all of the peptide sequences determined by amino acid sequencing analysis. There are two in-frame Met residues (-7 and -28) upstream of the N-terminus (as shown in SEQIDN03) of the mature protein which can serve as translation initiation sites. The nucleotide sequence surrounding the ATG codon for Met (-7) correlates better with the classical Kozak consensus (Kozak, 1989), but the nucleotide arrangement for Met (-28) strongly corresponds to starting points for at least two other known proteins which have been isolated from arachnids: Major house dust mite allergen (AAAATGA) (Yuuki et al , 1991 ) and Low molecular weight protein co-purified with o _Latrotoxin (AAATGA) (Kiyatkin et al, 1992) . In both cases, the deduced sequence preceding the N-terminus of the mature protein does not correspond to classical signal peptide structures. We conclude that post-translational modification of cT-LIT N-terminus is limited to removal of 7 or 28 amino acid residues. The existence of a cluster of positive amino acid residues Arg-X-Lys-Arg (-1-4) which can serve as a potential endopeptidase-cleavage site supports the hypothesis that post-translational processing occurs at the N-terminus.

Analysis of the deduced structure of c-f -LIT with PEST (Rogers, S. et al, 1986) reveals the presence of an

amino acid sequence enriched in P, E, S and T, which has previously been correlated with rapid degradation of intracellular proteins (Gottesmaπ & Mauπzi, 1992) . This region has the sequence EESGAPEGSFDSPSS, and is situated between residues 956-970. The presence of the PEST-region in the C-terminal part of _c -T-LIT is consistent with C-terminal processing of this protein.

Computer analysis of cf -LIT predicts three putative transmembrane helixes two of them situating in terminal regions (residues 39-67 and 221-240) and the third one of a minimal length (residues 580-595) being in the central region. The second putative transmembrane helix (residues 221-240) belongs to a very conservative region between all spider high molecular weight protein neurotoxins (Kiyatkin et al, 1993) .

Dot-matrix analysis of the predicted cT -LIT amino acid sequence revealed the presence of a repeated motif in the central part of the protein molecule. 460 amino acid residues of the ^f -LIT primary structure comprise tandemly arranged imperfect copies of the ankyrin-like repeats (Michaely & Bennett, 1992) . Whereas o< LT and ex -LIT (Kiyatkin et al, 1990, Kiyatkin et al, 1993) have no less than 20 repeats, «f -LIT has been found to have only 13 successive repeated units. Their optimal alignment is with phasing originally suggested in (Lux et

al, 1990). The sequence of 13 amino acids which precede the first repeat can be viewed as a reduced repeated unit according to its good correlation with a consensus sequence. The majority of -L U repeated units are 33-34 amino acids in length, but two repeats contain 35 (R1) and 36(R6) residues, respectively.

Analysis with the PCOMPARE programme showed the linear correlation between the repeated units of two insect-speci flc toxins. Strong linear correspondence has been found for eT-LIT repeats R2-R9 in comparison to the analogous repeats in e* -LIT (Kiyatkin et al, 1993). The first repeat in <f-LIT does not correspond well to the first one in «κ-LIT and shows high similarity to R7 from ex -LIT. «T-LIT repeat R10 is most similar to R19 from °< -LIT: this repeat is unusual in having Ser and Gly residues at position 8 and 31 , respectively. The next stretch of similarity is found between R11-R13 of J -L U and R10-R12 of o -LIT. We have noted that the R7, R2 and R9 repeats are the most highly conserved between the msectotoxins , suggesting a functional role in insectotoxicity . It has been shown that Erythrocyte Ankyπn repeats are not equivalent in respect of their functional ability to bind different proteins (Davis et al, 1991), and thus toxin repeats are also expected to make different contributions to their function.

Dot-matrix comparison of J ^" - and o< -LIT shows that they share a similar overall organization, with the strong central diagonal broken once (between 900 and 1130 amino acid residues of £ -LIT) and restored for the last 160 amino acids of both toxins. The displacement of the central diagonal reflects the difference in toxin length; cT-LIT is 190 amino acids shorter than its insect-speci flc counterpart.

The mature protein can be divided into several structural domains: an N-terminus consisting of about 470 amino acid residues and possessing strong linear homology with oc -LIT; the central domain of about 430 amino acids almost completely comprised of tande ly arranged ankyπn-like repeated units and a C-terminal domain of about 160 amino acids.

Alignment of the insectotoxin protein sequences shows that both the N- and C-terminal structural domains demonstrate the presence of high identity regions separated by rather dissimilar sequences, with a high level of identity (44.9% for the N-terminal domain and 37.1 ?ό for the C-terminus) . The most dramatic changes in primary structure of the two msectotoxins are concentrated in C-terminal parts of the repeat containing domains. A stretch of homology is localized to 13 ankyrin repeat units of ^ -LIT. This region is followed

by a sequence of about 110 amino acid residues that has no obvious homology either wιth<x -LIT or ©< LT nor with any other proteins from NBRF-PIR database. Interestingly, this domain, which is absent from c^ -LIT, forms a specific region in the primary structure of °<-LIT that has an unusual clustering of Cys-residues and possesses homology with mammalian-specific LT (Kiyatkin et al, 1993) . So striking structural difference between the two insect-speci flc neurotoxins suggests that the C-terminal part of the ankyrin-like repeated domain plays a particular role in providing a structural basis for their different functional properties.

The high molecular weight protein toxins from the venom of the Black Widow Spider are a potent and specific class of toxins. These toxins offer a great potential for elucidating the function of neural proteins, and for providing insect specific toxins. However this potential has not previously been realised due to the inability to express these protein toxins with any function. The present invention provides for the cloning of a novel DNA transcript encoding for a novel msect-speci fIC toxin, and functional expression of this toxin, and other polypeptides in bacteria.

The © -LIT cDNA was cloned with an oligonucleotide based on the sequence of amino acids 1-23 of the toxin,

and its identity confirmed by additional peptide sequences, and immunochemical identity, using an antibody specific for the -LIT. The deduced primary structure of _* S-LIT has considerable similarity to the mammalian specific ^LT and the insect-specific o<-LIT, suggesting that these toxins are part of a family with similar structure. The three proteins have a central domain which is composed of "ankyrin-like" repeats, with 13 repeats in <S - L U . The ankyrin family of proteins couple spectrin to a variety of integral membrane proteins (Bennett, 1992), and it is believed that the "ankyrin repeat" domain of the ankyrins is responsible for specific binding to proteins (Davis and Bennett, 1990 J Biol Chem 265: 10589-10596; Davis et al (1991) J. Biol Chem 266: 11163-11169). This structural similarity with the ankyrin family is reflected in the known functional properties of the latrotoxins; c LT is known to bind to a receptor with high affinity (K , 10 M). It remains to be determined whether this specific binding to theo LT receptor is mediated via the ankyrin repeat region of the toxin.

Surprisingly, cS -LIT has no greater similarity to the insect-specific c -LIT (38?ό) than to the mammalian- specific c LT (37?ό ) . Whereas oT -LIT has only 13 repeats, thec< LT and c -LIT have 19 and 20 ankyrin repeats, respecti ely. The latter 6/7 repeats have no counterpart

in the c^T-LIT, and may be a structural unit, as the "^ - toxins both contain 6 cysteine residues in this region, with partially conserved spacing. However, in view of the differences in target toxicity of the «=>< LT and o -LIT, it is not possible to identify this structural features with insect-speci fIC toxicity.

s -LIT exhibits a marked disparity between the molecular weight of the toxin, as deduced from the cDNA sequence, and the relative mobility of the pure toxin purified from venom. Whilst the N-terminus of the protein has been identified unambiguously by protein sequencing, the precise position of the C-terminus has been difficult to document. Expression of the full length es> -LIT cDNA in bacteria demonstrated that the calculated molecular mass is accurately reflected in the relative mobility of the protein on SDS-PAGE, and that the natural venom derives predominantly, if not wholly from proteolytic, C-terminal processing. The full-length recombinant protein was purified, but was not toxic to locusts under any conditions. The full-length protein is an inactive precursor of the functional toxin.

The precise site of the C-terminus of ^« -> -LIT purified from venom was assessed by MALDI- ass spectrometry , which localised the site of cleavage to amino acid 991 of the protein. The cDNA was mutated to

produce a protein of 991 amino acids with a sequence as shown in SEQIDN02, and expressed in bacteria. The mature recombinant protein was soluble and was lethal to locusts. Partial purification of the protein suggests that the toxin is highly toxic.

Expression of the mature toxin from using the truncated form of the full gene sequence as described above has considerable advantages. Firstly, the toxin can be produced relatively easily by functional expression of the truncated form in a bacterial system, thereby obviating the need to purify toxin from venom glands of spiders. This enables industrial production of the toxin and hence commercial exploitation, for example as the major component of an insecticide system.

Moreover, it presents possible administration systems for the toxin as an insecticide, beside conventional methods such as spraying. For example it may be possible to produce a modified plant cell or plant, such as a crop plant, containing a recombinant molecule incorporating the truncated sequence. Such a system comprises a recombinant baculovirus comprising the truncated form. Such viruses are highly infectious in vivo and resistant to inactivation in host cells, and are capable of high levels of expression of the inserted nucleotide sequence in host insect cells. This is expected to be harmless to the plant and indeed to

vertebrates. Upon ingestion of the plant tissue an insect will take in the recombinant molecule and/or toxin, resulting ultimately in the death of the insect. Since the toxin is insect specific, it is expected to have no detrimental effect to humans or animals upon consumption.

This is an example of one use of one embodiment of the invention to express a toxin that is usually produced by post-translational modification of a precursor protein in biological systems, in a bacterial expression system. It is to be appreciated that the truncated form of other genes coding for other proteins could be expressed in this way, and fall within the scope of the present invention .

The invention also provides toxin formed from the expression of a full, isolated gene to produce a precursor polypeptide which is then post-translationally modified. The precursor polypeptide has an amino acid sequence as shown in SEQIDN04, and the toxin has a sequence as shown in SEQIDN02.

The isolated gene (SEQIDN03) (or an analogue) encoding for the precursor polypeptide of the toxin ef LIT can be cloned into a vector for expression of the precursor polypeptide. A baculovirus expression system

can be used. The precursor polypeptide thus produced can then be truncated at the sites indicated above, by site directed mutagenesis, to produce an active toxin. This enables the toxin efLIT or an active derivative thereof, to be produced independently of the Black Widow Spider, and thus on an industrial scale, for use as indicated as an insecticide.

Whilst endeavouring in the foregoing specification to draw attention to those features of the invention believed to be of particular importance it should be understood that the Applicant claims protection in respect of any patentable feature or combination of features hereinbefore referred to and/or shown in the drawings whether or not particular emphasis has been placed thereon .

SEQUENCE LISTING

(1 ) GENERAL INFORMATION:

(i ) APPLICANT:

(A) NAME: BRITISH TECHNOLOGY GROUP LIMITED

(B) STREET: 101 NEWINGTON CAUSEWAY

(C) CITY: LONDON

(E) COUNTRY: UNITED KINGDOM

(F) POSTAL CODE (ZIP) : SET 6BU

(ii) TITLE OF INVENTION: A NOVEL TOXIN AND A METHOD OF PRODUCING A TOXIN

(i NUMBER OF SEQUENCES: A

(iv) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Floppy disk

(B) COMPUTER: IBM PC compatible

CO OPERATING SYSTEM: PC-DOS/MS-DOS

(D) SOFTWARE: Pateπtln Release £1.0, Version £1.30 (EPO)

(2) INFORMATION FOR SEQ ID NO: 1:

(i ) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2976 base pairs (3) TYPE: nucleic acid

(C) STRANDEDNESS: double (0) TOPOLOGY: unknown

(ii ) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "PLASMID DNA"

(vi ) ORIGINAL SOURCE:

(A) ORGANISM: LATRODECTUS MACTANS TREDECIMGUTTATUS

(vii) IMMEDIATE SOURCE:

(B) CLONE: pT7.del aM

(ix) FEATURE:

(A) NAME/ EY: COS (3) LOCATION:! ..2976

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1 :

GAT GAA GAA GAT GGA GAA ATG ACT CTA GAA GAA AGA CAA GCA CAA TGC 4

. Asp Glu Glu Asp Gly Glu Met Thr Leu Gl u Glu Arg Gin Ala Gin Cys 1 5 10 15

AAA GCA ATA GAG TAC AGC AAT TCA GTT TTT GGG ATG ATC GCT GAT GTA 9

Lys Ala lie Glu Tyr Ser Asπ Ser Val Phe Gl Met lie Ala Asp Val 20 25 30

GCT AAC G GTT GGC ATT 1 -

Ala Asn A Val Gly Il _e

GTA ACT GCC CCA ATT GCC ATC GTA AGT CAC ATT ACT AGC GCA GGC TTG ^1S

Val Thr Ala Pro He Ala lie Val Ser His lie Thr Ser Ala Gly Le> 50 55 60

24

65 70 - 32 75 80

2sa

Imr

432

TTC AAA ATA AAT GAT TTT AAA AAG TTT TTT GAA AAA GAA CGA CAA AGA 480 Phe Lys lie Asn Asp Phe Lys Lys Phe Phe Glu Lys Glu Arg Gin Arg 145 150 155 isα

ATT AAA GGT TTG CCT AAA GAT AGG TAT GTT GCT AAG CTT CTA GAA CAA S2S lie Lys Gly Leu Pro Lys Asp Arg Tyr Val Ala Lys Lsu Lsu Glu Gin 155 170 175

AAA GGT ATT TTA GGT TCT TTA AAA GAA GTA AGA GAA CCA TCT GGA AAC Lys Gly lie Leu Gly Ser Lsu Lys Glu Val Arg Glu Pro Ser Gly Asn ISO 1S5 190

5 ^;

672

720

763

815

8S4

912

950

GAG ACT CTT AAA AAT CAA ATC AAA ACG ACT GAT T.G CCT CTT ATA GAT -■coε Glu ~-r Leu Lys Asn Gin He Lys T r Tnr Asp Leu P o Leu II e Asp 325 330 335

GAT ATA CCC GAA ACT TTG TCT CAA GTG AAC TTT CCG AAT GAC GAA AAT 1Q _S6

Asp lie Pro Glu Thr Leu Ser Gin Val Asn Phe Pro Asn Asa Glu Asn 340 345 ₃₅₀

CAA TTG CCT ACA CCA ATA GGA AAT TGG GTT GAT GGC GTA GAA GTT AGG 11Q ₄

Gin Leu Pre Thr Pro lie Gly Asn Trp Val Asp Gly Val Glu Val Arg 355 350 365

TAC GCA GTA CAG TAT GAA AGT AAG GGC ATG TAT TCG AAA TTC AGT GAA 11=2

Tyr Ala Val Gin Tyr Glu Ser Lys Gly Met Tyr Ser Lys Phe Ser Glu 370 375 380

TGG TCT GAA CCA TTT ACT GTC CAA GGT AAC GCT TGT CCG ACT ATA AAA 1200

Trp Ser Glu Pro Phe Thr Val Gin Gly Asn Ala Cys Prp Thr Il _s Lys 385 390 395 ^" 400

GTT CGT GTT GAT CCG AAA AAG AGA AAT AGA CTT ATC TTT AGG AAG TTC 1248

Val Arg Val Asp Pro Lys Lys Arg Asn Arg Leu lie Phe Arg Lys Phe 405 410 415

AAC TCA GGA AAA CCT CAG TTT GCT GGA ACC ATG ACT CAT TCA CAA ACA 1296

Asn Ser Gly Lys Pro Gin Phe Ala Gly Thr Met Thr His Ser Gin Thr 420 425 430

AAT TTT AAA GAT ATT CAT CGT GAT CTA TAC GAT GCA GCC TTA AAT ATT 1344

Asn Phe Lys Asp lie His Arg Asp Leu Tyr Asp Ala Ala Leu Asn lie 435 440 445

AAT AAG TTG AAA GCA GTG GAT GAA GCT ACA ACT TTG ATT GAA AAG GGT 1392

Asn Lys Leu Lys Ala Val Asp Glu Ala Thr Thr Leu l e Glu Lys Gly 450 455 460

GCA GAC ATA GAA GCA AAA TTT GAC AAT GAC AGA AGT GCA ATG CAC GCA 1440

Ala Asp lie Glu Ala Lys Phe Asp Asn Asa Arg Ser Ala Met His Ala 4S5 470 475 480

GTT GCA TAT CGA GGA AAT AAC AAA ATA GCC TTA AGA TTT CTT TTG AAA 1488

Val Ala Tyr Arg Gly Asn Asn Lys ie Ala Leu Arg Phe Leu Lau Lys 485 4S0 495

AAT CAA TCC ATT GAC ATC GAG TTA AAA GAT AAA AAC GGC TTT ACT CCT 1536

Asn Gin Ser lie Asp lie Glu Leu Lys Asp Lys Asn Gly Phe Thr Pro 500 505 510

CTA CAC ATC GCA GCT GAA GCA GGT CAG GCA GGA TTT GTT AAG TTA CTA 1584

Leu His lie Ala Ala Glu Ala Gly Gin Ala Gly Phe Val Lys Lsu Leu 515 520 525

ATA AAT CAT GGA GCT GAT GTG AAT GCA AAA ACA AGT AAG ACA AAT TTG 1632 lie Asn His Gly Ala Asp Val Asn Ala Lys Thr Ser Lys Thr Asn Leu 530 535 540

ACA CCA TTA CAT CTT GCA ACA CGT AGT GGA TTT TCA AAA ACT GTA AGA 1680

Thr Pro Leu His Leu Ala Thr Arg Ser Gly Phe Ser Lys Thr Val Arg 545 550 555 560

AAT TTA CTA GAA AGC CCA AAT ATT AAG GTA AAT GAA AAG GAG GAT GAC 1 2

Asn Leu Leu Glu Ser Pro Asn He Lys Val Asn Glu Lys Glu Asp Asa 565 570 575

GGA TTT ACA CCT TTG CAT ACT GCA GTA ATG AGT ACT TAT ATG GTT GTC 177

G1y Phe Thr Pro Leu His Thr Ala Val Met Ser Thr Tyr Met Val Val 530 585 590

GAT GCT TTG CTA AAT CAT CCA GAC ATT GAT AAA AAT GCG CAG TCT ACG 182

Asp Ala Leu Leu Asn His Pre Asp lie Asp Lys Asn Ala Gin Sar T r

suBSϊiτu MFiΞT fP

595 600 - 34 - ₆₀₅

TCA GGA TTG ACT CCT TTC CAT TTA GCA ATT ATT AAT GAA AGT CAA GAA 1372 Ser Gly Leu Thr Pro Phe His Leu Ala lie lie Asn Glu Ser Gin Glu 610 615 620

GTT GCA GAA TCT TTA GTG GAA AGT AAT GCT GAT CTA AAT ATT CAG GAT 1920 Val Ala Glu Ser Leu Val Glu Ser Asn Ala Asp Leu Asn lie Gin Asp 625 630 635 640

GTT AAC CAT ATG GCT CCT ATT CAT TTT GCA GCT TCA ATG GGT AGT ATT 1968 Val Asn His Met Al Pro lie His Phe Al Ala Ser Met Gly Ser lie 645 650 655

AAA ATG CTT AGA TAT CTC ATT TCC ATA AAA GAT AAA GTT AGT ATT AAT 2016 Lys Met Leu Arg Tyr Leu lie Ser lie Lys Asp Lys Val Ser lie Asn 660 665 670

TCT GTG ACT GAG AAT AAT AAC TGG ACA CCT TTA CAT TTT GCT ATA TAT 2064 Ser Val Thr Glu Asn Asn Asn Trp Thr Pro Leu His Phe Ala lie Tyr 675 680 685

TTT AAA AAA GAA GAT GCT GCA AAA GAA TTG TTG AAA CAA GAT GAC ATA 2112 Phe Lys Lys Glu Asp Al Al Lys Glu Leu Leu Lys Gin Asp Asp lie 690 695 700

AAT TTA ACA ATT GTT GCA GAT GGT AAT CTT ACC GTT TTA CAT CTT GCT 2160 Asn Leu Thr lie Val Al Asp Gly Asn Leu Thr Val Leu His Leu Ala 705 710 715 720

GTT TCG ACA GGA CAA ATA AAT ATA ATT AAA GAA TTA TTG AAG AGA GGC 2208 Val Ser Thr Gly Gin lie Asn lie lie Lys Glu Leu Leu Lys Arg Gly 725 730 735

TCC AAT ATA GAA GAA AAA ACT GGA GAA GGA TAT ACA TCT CTC CAC ATC 2256 Ser Asn lie Glu Glu Lys Thr Gly Glu Gly lyr Thr Ser Leu His lie 740 745 750

GCT GCG ATG CGA AAG GAG CCA GAG ATA GCT GTT GTT TTG ATT GAA AAC 2304 Ala Ala Met Arg Lys Glu Pro Glu ie Ala Val Val Leu lie Glu Asn 755 760 765

GGT GCT GAC ATA GAA GCT CGA TCA GCT GAT AAT TTA ACA CCT TTA CAT 2352 Gly Ala Asp lie Glu Ala Arg Ser Ala Asp Asn Leu Thr Fro Leu His 770 775 780

TCT GCC GCA AAA ATA GGA AGG AAA TCT ACA GTA CTT TAC TTA TTA GAA 2400 Ser Ala Ala Lys lie Gly Arg Lys Ser Thr Val Leu Tyr Leu Leu Glu 785 790 795 800

.AAA GGA GCT GAC ATT GGA GCT AAA ACA GCA GAC GGT TCT ACT GCC TTG 2448 Lys Gly Ala Asp lie Gly Ala Lys Thr Ala Asp Gly Ser Thr Ala Leu 805 810 815

CAT TTA GCT GTA TCT GGT CGT AAA ATG AAA ACT GTT GAA ACT CTA TTA 2496 His Leu Ala Val Ser Gly Arg Lys Met Lys Thr Val Glu Thr Leu Leu 820 825 830

AAT AAA GGA GCA AAT TTA AAA GAA TAC GAT AAC AAT AAA TAT TTG CCA 2544 Asn Lys Gly Ala Asn Leu Lys Glu Tyr Asp Asn Asn Lys Tyr Leu Pro 835 840 845

ATA CAT AAA GCT ATT ATT AAT GAT GAC CTT GAC ATG GTA CGT TTG TTT 2592 lie His Lys Ala lie lie Asn Asp Asp Leu Asp Met Val Arg Leu Phe 850 855 860

CTT GAA AAA GAT CCC AGT CTC AAA GAT GAT GAA ACA GAA G _AG GGT AGA 2 _S40

Leu Glu Lys Asp Pro Ser Leu Lys Asp AS _P Glu ihr Gl _u Q _{1U Gly} , p,-g 865 870 875 880

ACT TCA ATT ATG TTA ATT GTT CAG AAA TTG CTT CTT GAA TTA _TA T AAC 2S8a Thr Ser lie Met Leu lie Val Gin Lys Leu Leu Leu Glu Leu _Ty r Asn 885 890 895

TAT TTT ATA AAT AAT TAT GCT GAA ACT TTG GAT GAA GAA GCT TT _A TTC 2735 Tyr Phe lie Asn Asn Tyr Ala Glu Thr Leu Asp Glu Glu Ala Leu Phe 900 905 910

AAC CGC TTA GAT GAA CAA GGG AAA TTA GAG CTT GCA TAT ATC TTC CAT 273-1 Asn Arg Leu Asp Glu Gin Gly Lys Leu Glu Leu Ala Tyr π _e Phe His 9T5 920 925

AAT AAA GAA GGT GAT GCA AAA GAG GCT GTT AAG CCA ACT ATC CTT GTT 2832 Asn Lys Glu Gly Asp Ala Lys Glu Ala Val Lys Pro Thr lie Leu Val 930 935 340

ACA ATT AAA CTT ATG GAA TAC TGC TTA AAA AAA CTT CGC GAA GAG TCT 2880 Thr lie Lys Leu Met Glu Tyr Cys Leu Lys Lys Leu Arg Glu Glu Ser 945 950 355 960

GGA GCT CCT GAA GGT AGT TTC GAT TCT CCA TCT TCA AAG CAA TGT ATT 2323 Gly Ala Pro Glu Gly Ser Phe Asp Ser Pro Ser Ser Lys Gin Cys lie 955 S70 375

TCT ACC TTT TCA GAG GAT GAA ATG TTT CGT CGT ACT TTA CCS GAA TGA 2376 Ser Thr Phe Ser Glu Asp Glu Met Phe Arg Arg Thr Leu Pro Glu * 980 985 990

(2) INFORMATION rOR SEQ ID NO: 2:

Ci) SEQUENCE CHARACTERISTICS: (A) LENGTH: 932 amino ac s C≥) TYPE: am no acii (0) TOPOLOGY: l near

Cϋ) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2:

Asp Glu Glu Asp Gly Glu Met Thr Leu Glu Glu Arg Gin Ala Gin Cys

1 5 10 15

Lys Ala ie Glu Tyr Ser Asn Ser Val Phe Gly Met ie Ala Asp Val 20 25 30

Ala Asn Asp lie Gly Ser lie Pro Val lie Gly Glu Val Val Gly lie 35 40 45

Val Thr Ala Pro lie Ala lie Val Ser His lie Thr Ser Ala Gly Leu 50 55 60

Asp ie Ala Ser Thr Ala Leu Asp Cys Asp Asp ie Pro Phe Asp Glu 55 70 75 80 lie Lys Gl ie Leu Glu Glu A.-g Phe Asn Glu lie Asp Arg Lys Leu 85 90 95

Asp Lys Asn Thr Ala Ala Leu Glu Glu Val Ser Lys Leu Val Ser Lys

-.oo ι c 5 no

Thr Pne Val Thr Val Glu Lys Thr Arg Asn Glu Met Asn Glu Asn Phe ITS 120 125

SUBSTITUTE SHEET (RULE 26}

Lys Leu Val Leu Glu Thr lie Glu Ser Lys Glu lie Lys Ser lie Val 130 135 . 1 0

Phe Lys lie Asn Asp Phe Lys Lys Phe Phe Glu Lys Glu Arg Gin Arg 145 150 ^* 155 160 lie Lys Gly Lsu Pro Lys Asp Arg Tyr Val Ala Lys Leu Leu Glu Gin 165 170 ₁₇₅

Lys Gly lie Leu Gly Ser Leu Lys Glu Val Arg Glu Pro Ser Gly Asn 180 185 190

Ser Leu Ser Ser Ala Leu Asn Glu Leu Leu Asp Lys Asn Asn Asn Tyr 195 200 205

Ala lie Pro Lys Val Val Asp Asp Asn Lys Ala Phe Gin Ala Leu Tyr 210 215 220

Ala Leu Phe Tyr Gly Thr Gin Thr Tyr Ala Ala Val Met Phe Phe Leu 225 230 235 240

Leu Glu Gin His Ser Tyr Leu Ala Asp Tyr Tyr Tyr Gin Lys Gly Asp 245 250 255

Asp Val Asn Phe Asn Al Glu Phe Asn Asn Val Ala lie He Phe Asp 2S0 265 270

Asp Phe Lys Ser Ser Leu Thr Gly Gly Asp Asp Gly Lsu lie Asp Asn 275 23Q 285

Val lie Glu Val Leu Asn Thr Val Lys Ala Leu Pro Phe lie Lys Asn 290 295 300

Ala Asp Ser Lys Leu Tyr Arg Glu Leu Val Thr Arg Thr Lys Ala Leu 305 310 315 320

Glu Thr Leu Lys Asn Gin lie Lys Thr Thr Asp Leu Pro Leu lie Asp 325 330 335

Asp lie Pro Glu Thr Leu Ser Gin Val Asn Phe Pro Asn Asp Glu Asn 340 345 350

Gin Leu Pro Thr Pro lie Gly Asn Trp Val Asp Gl Val Glu Val Arg 355 360 365

Tyr Ala Val Gin Tyr Glu Ser Lys Gly Met Tyr Ser Lys Phe Ser Glu 370 375 380

Trp Ser Glu Pro Phe Thr Val Gin Gly Asn Ala Cys Pro Thr lie Lys 385 390 395 400

Val Arg Val Asp Pro Lys Lys Arg Asn Arg Leu lie Phe Arg Lys Phe

405 410 415

Asn Ser Gly Lys Pro Gin Phe Ala Gly Thr Met Thr His Ser Gin Thr 420 425 430

Asn Phe Lys Aso lie His Arg Asp Leu Tyr Asp Ala Ala Leu Asn lie 435 440 445

Asn Lys Leu Lys Ala Val Asp Glu Ala Thr Thr Leu Ila Glu Lys Gly

450 455 460

_A la _A sp lie Glu Ala Lys Phe Asp Asn Asp Arg Ser Ala Met His Ala 465 470 ⁴⁷ 5 480

SUES ! ^" Ξ SHEET (RULE 26 j

Val Ala Tyr Arg Gly Asn Asn Lys lie Al _a Leu Arg Phe Leu Leu Lys 435 490 495

Asn Gin Ser lie Asp lie Glu Leu Lys Asp Lys Asn Gly Phe Thr Pre 500 505 510

Leu His lie Ala Ala Glu Ala Gly Gin Al Gly Phe Val Lys Leu Leu 515 520 525 lie Asn His Gly Ala Asp Val Asn Ala Lys Thr Ser Lys Thr Asn Leu 530 535 540

Thr Pro Leu His Leu Ala Thr Arg Ser Gly Phe Ser Lys Thr Val Arg 545 550 5S5 560

Asn Leu Leu Glu Ser Pro Asn lie Lys Val Asn Glu Lys Glu Asp Asp 565 570 575

Gly Phe Thr Pro Leu His Tnr Ala Val Met Ser Tnr Tyr Met Val Val

580 585 590

Asp Ala Leu Leu Asn His Pro Asp lie Asp Lys Asn Ala Gin Ser Thr

595 600 . 605

Ser Gly Lsu Thr Pro Phe His Leu Ala lie lie Asn Glu Ser Gin Glu 610 615 620

Val Ala Glu Ser Lsu Val Glu Ser Asn Ala Asp Leu Asn lie G n Asp 625 630 635 540

Val Asn H s Me- Ala Pro lie His rhe Ala Ala Ser Met Gly Ser Us 645 650 655

Lys Met Lsu Arg Tyr Leu He Ser lie Lys Asp Lys Val Ser He Asn

660 655 67Q

Ser Val Thr Glu Asn Asn Asn Trp Thr Pro Leu His Fhe Ala lie Tyr 675 680 635

Phe Lys Lys Glu Asp Ala Ala Lys Gl Leu Leu Lys G n Asp Asp lie 690 695 700

Asn Leu T r lie Val Ala Asp Gly Asn Leu Thr Val Lsu His Leu Ala 705 710 715 720

Val Ser Thr Gly Gin lie Asn lie lie Lys Glu Leu Leu Lys Arg Gly 725 730 735

Ser Asn lie Glu Glu Lys Thr Gly Glu Gly Tyr Thr Ser Leu His lie 740 745 750

Ala Ala Met Arg Lys Glu Pro Glu lie Ala Val Val Leu lie Glu Asn

755 760 765

Gly Ala Asp lie Glu Al A g Ser Al Asp Asn Leu Thr Prα Leu Kis 770 775 7S0

Ser Ala Ala Lys lie Gl Arg Lys S=r Val Leu ιyr Leu Leu G u

790 / -53 SOC

Lys Gly _A la Asp lie Gly Ala Lys Thr Ala Asp Gly S≤r Thr Ala Leu 805 S10 8T5

_H is Leu _A la _V al Ser Gly Arς Lys Mat Lys Thr Val Glu Thr L≥U Leu

820 825 εαα

SUBSTITUTE SHEET (RULE 26

sn Lys Gly Ala Asn Leu Lys Glu T "yyrr- Asp Asn Asn Lys Tyr Leu Pro

835 340 845 lie H s Lys Al He He Asn Asp Asp Leu Asp Met Val Arα Leu Phe 850 855 860

Leu Glu Lys Asp Pro Ser Leu Lys Asp Asp Glu Thr Glu Glu Gly Arg 865 870 875 _8a0

Thr Ser lie Met Leu lie Val Gin Lys Leu Leu Leu Glu Leu Tyr Asn

885 890 895

Tyr Phe lie Leu Phe

Asn Arg Leu Asp Glu Gin Gly Lys Leu Glu Leu Al Tyr lie Phe His 915 920 925

Asn Lys Glu Gly Asp Ala Lys Glu Ala Val Lys Pro Thr lie Leu Val 930 935 940

Thr lie Lys Leu Met Glu Tyr Cys Leu Lys Lys Leu Arg Glu Glu Ser 94.5 950 955 960

Gly Ala Pro Glu Gly Ser Phe Asp Ser Pro Ser Ser Lys Gin Cys lie 965 970 975

Ser Thr Phe Ser Glu Asp Glu Met Phe Arg Arg Thr Leu Pro Glu * 930 985 990

(2) INFORMATION FOR SΞQ ID NO: 3:

Ci) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 3706 base pai s (5) TYPE: nucleic acid CO STRANDEDNΞS3: double CO) TOPOLOGY: c rcular

Ci ) MOLECULE TYPE: other nucleic acid

CA) DESCRIPTION: /desc = "PLASMID DNA"

Cvi) ORIGINAL SOURCE:

CA) ORGANISM: LATRODECTUS MACTANS TREDECIMGUTTATUS

Cv i) IMMEDIATE SOURCE:

CB) CLONE: pT7.deltaFL

Cix) FEATURE: A) NAME/KEY: CDS

CB) LOCATION:45..3686

Cxi) SEQUENCE DESCRIPTION: SΞQ ID NO: 3:

GGTCAATTGA AACTTTATGA TAGGATTCAC TTTCTTATAT AGAA ATG CAT TCC AAA 56

Met His Ser Lys 995

GAA TTA CAA ACT ATT TCA GCA GCG GTA GCA CGA AAA GCA GTA CCC AAT 104 Glu Leu Gin Thr lie Ser Ala Ala Val Ala Arg Lys Ala Val Pro Asn 1000 IOCS 1010

_A CT _A TG GTT ATT CGG TTG AAA AGA GAT GAA GAA GAT GGA GAA ATG ACT 1=2 Thr Met Val lie Arς Leu Lys Arg Asp Glu G u Asp Gly Glu Met Thr

SHEET (RULE 26j

1015 1020 1025

CTA GAA GAA AGA CAA GCA CAA TGC AAA GCA ATA GAG TAC AGC AAT TCA 200 Leu Glu Glu Arg Gin Ala Gin Cys Lys Ala lie Glu Tyr Ser Asn Ser 1030 1035 1040

^" GTT TTT GGG ATG ATC GCT GAT GTA GCT AAC GAC ATC GGT TCC ATT CCT 248 Val Phe Gly Met lie Ala Asp Val Ala Asn Asp lie Gly Ser He Pro 1045 1050 1055 ι ₀ 60

GTA ATT GGC GAA GTA GTT GGC ATT GTA ACT GCC CCA ATT GCC ATC GTA 296 Val lie Gly Glu Val Val Gly lie Val Thr Ala Pro He Ala He Val 1065 1070 1075

AGT CAC ATT ACT AGC GCA GGC TTG GAT ATA GCT TCT ACG GCA TTA GAT 344 Ser His lie Thr Ser Ala Gly Leu Asp lie Ala Ser Thr Ala Leu Asp 1080 1085 1090

TGT GAT GAT ATA CCT TTT GAT GAG ATT AAG GAA ATA TTA GAA GAA AGA 392 Cys Asp Asp lie Pro Phe Asp Glu lie Lys Glu lie Leu Glu Glu Arg 1095 1100 1105

TTC AAT GAA ATA GAT AGA AAG TTG GAC AAG AAC ACA GCT GCT TTG GAA 440 Phe Asn Glu ie Asp Arg Lys Leu Asp Lys Asn Thr Ala Ala Leu Glu 1110 1115 1120

GAG GTC TCT AAA CTG GTA AGT AAA ACT TTT GTT ACG GTG GAA AAA ACA 488 Glu Val Ser Lys Leu Val Ser Lys Thr Phe Val Thr Val Glu Lys Thr 1125 1130 1135 1140

AGG AAT GAA ATG AAC GAA AAT TTT AAG CTT GTT TTG GAA ACT ATA GAA 536 Arg Asn Glu Met Asn Glu Asn Phe Lys Leu Val Leu Glu Thr lie Glu 1145 1150 1155

AGC AAA GAA ATA AAA TCA ATT GTA TTC AAA ATA AAT GAT TTT AAA AAG 584 Ser Lys Glu lie Lys Ser lie Val Phe Lys lie Asn Asp Phe Lys Lys 1160 1165 1170

TTT TTT GAA AAA GAA CGA CAA AGA ATT AAA GGT TTG CCT AAA GAT AGG 632 Phe Phe Glu Lys Glu Arg Gin Arg lie Lys Gly Leu Pro Lys Asp Arg 1175 1180 1185

TAT GTT GCT AAG CTT CTA GAA CAA AAA GGT ATT TTA GGT TCT TTA AAA 680 Tyr Val Ala Lys Leu Leu Glu Gin Lys Gly lie Leu Gly Ser Leu Lys 1190 1195 1200

GAA GTA AGA GAA CCA TCT GGA AAC AGT CTG AGC TCC 6CG TTA AAT GAA 728 Glu Val Arg Glu Pro Ser Gly Asn Ser Leu Ser Ser Ala Leu Asn Glu 1205 1210 1215 1220

CTC TTA GAC AAA AAC AAC AAC TAT GCC ATC CCA AAA GTG GTT GAT GAT 776 Leu Leu Asp Lys Asn Asn Asn Tyr Ala lie Pro Lys Val Val Asp Asp 1225 1230 1235

AAT AAG GCC TTT CAG GCG CTG TAT GCT TTA TTT TAT GGA ACT CAG ACT 824 Asn Lys Ala Phe Gin Ala Leu Tyr Ala Leu Phe Tyr Gly Thr Gin Thr 1240 1245 1250

TAT GCA GCC GTT ATG TTT TTC TTA CTC GAA CAA CAT TCT TAT CTG GCT 872 Tyr Ala Ala Val Met Phe Phe Leu Leu Glu Gin His Ser Tyr Leu Al 1255 1260 1265

GAT TAT TAT TAC CAA AAA GGT GAT GAT GTA AAT TTT AAT GCA GAA TTT S20 Asp Tyr Tyr Tyr Gin Lys Gly Asp Asp Val Asn Phe Asn Ala Glu Phe 1270 1275 1280

AAT AAT GTA GCA ATT ATT TTT GAT GAC TTT AAA TCA TCA CTA ACA GGA 968

Asn Asn Val Al a l i e l i e Phe Asp Asp Phe Lys Se r Ser Leu Thr Gl y 1 285 1290 1 295 1300

GGA GAT GAC GGA TTA ATA GAT AAT GTC ATT GAG GTT CTT AAC ACC GTG 1016 Gly Asp Asp Gly Leu lie Asp Asn Val lie Glu Val Leu Asn Thr Val 1305 1310 1315

AAA GCA TTA CCA TTT ATA AAG AAC GCC GAC AGT AAA CTA TAC AGA GAA 1064 Lys Ala Leu Pro Phe lie Lys Asn Ala Asp Ser Lys Leu Tyr Arg Glu 1320 1325 1330

TTA GTA ACT AGA ACA AAA GCT TTA GAG ACT CTT AAA AAT CAA ATC AAA 1112 Leu Val Thr Arg Thr Lys Ala Leu Glu Thr Leu Lys Asn Gin lie Lys 1335 1340 1345

ACG ACT GAT TTG CCT CTT ATA GAT GAT ATA CCC GAA ACT TTG TCT CAA 1160 Thr Thr Asp Leu Pro Leu lie Asp Asp lie Pro Glu Thr Leu Ser Gin 1350 ^• 1355 1360

GTG AAC TTT CCG AAT GAC GAA AAT CAA TTG CCT ACA CCA ATA GGA AAT 1208 Val Asn Phe Pro Asn Asp Glu Asn Gin Leu Pro Thr Pro lie Gly Asn 1365 1370 1375 1380

TGG GTT GAT GGC GTA GAA GTT AGG TAC GCA GTA CAG TAT GAA AGT AAG 1256 Trp Val Asp Gly Val Glu Val Arg Tyr Ala Val Gin Tyr Glu Ser Lys 1385 1390 1395

GGC ATG TAT TCG AAA TTC AGT GAA TGG TCT GAA CCA TTT ACT GTC CAA 1304 Gly Met Tyr Ser Lys Phe Ser Glu Trp Ser Glu Pro Phe Thr Val Gin 1400 1405 1410

GGT AAC GCT TGT CCG ACT ATA AAA GTT CGT GTT GAT CCG AAA AAG AGA 1352 Gly Asn Ala Cys Pro Thr lie Lys Val Arg Val Asp Pro Lys Lys Arς 1415 1420 1425

AAT AGA CTT ATC TTT AGG AAG TTC AAC TCA GGA AAA CCT CAG TTT GCT 1 00 Asn Arg Leu lie Phe Arg Lys Phe Asn Ser Gly Lys Pro Gin Phe Ala 1430 1435 1440

GGA ACC ATG ACT CAT TCA CAA ACA AAT TTT AAA GAT ATT CAT CGT GAT 1 48 Gly Thr Met Thr His Ser Gin Thr Asn Phe Lys Asp lie His Arg Asp 1445 1450 1455 1460

CTA TAC GAT GCA GCC TTA AAT ATT AAT AAG TTG AAA GCA GTG GAT GAA 1 96 Leu Tyr Asp Ala Ala Leu Asn He Asn Lys Leu Lys Ala Val Asp Glu 1465 1470 1475

GCT ACA ACT TTG ATT GAA AAG GGT GCA GAC ATA GAA GCA AAA TTT GAC 1544 Ala Thr Thr Leu lie Glu Lys Gly Ala Asp lie Glu Ala Lys Phe Asp 1480 1485 1490

AAT GAC AGA AGT GCA ATG CAC GCA GTT GCA TAT CGA GGA AAT AAC AAA 1592 Asn Asp Arg Ser Ala Met His Ala Val Ala Tyr Arg Gly Asn Asn Lys 1495 1500 1505

ATA GCC TTA AGA TTT CTT TTG AAA AAT CAA TCC ATT GAC ATC GAG TTA 1640 lie Al Leu Arg Phe Leu Leu Lys Asn Gin Ser lie Asp lie Glu Leu 1510 1515 1520

AAA GAT AAA AAC GGC TTT ACT CCT CTA CAC ATC GCA GCT GAA GCA GGT 1688 Lys Asp Lys Asn Gly Phe Thr Pro Leu His lie Ala Ala Glu Ala Gly 1525 1530 1535 1540

CAG GCA GGA TTT GTT AAG TTA CTA ATA AAT CAT GGA GCT GAT GTG AAT 1 36 Gin Ala Gly Phe Val Lys Leu Leu lie Asn His Gly Ala As ^p Val Asn

SUBS! MoiESHEE ^"1 1 r rr

Vxj c i-O)

GCA AAA ACA CA CGT 1~c ₄ Ala Lys Thr hr Arg

AGT GGA TTT TCA AAA ACT GTA AGA AAT TTA CTA GAA AGC CCA AAT ATT 1532 Ser Gly Phe Ser Lys T r Val Arg A≤n Leu Leu Glu Ser Pro Asn ie 1575 1530 1585

AAG GTA AAT GAA AAG GAG GAT GAC GGA TTT ACA CCT TTG CAT ACT GCA 1380 Lys Val Asn Glu Lys Glu Asp Asp Gly Phe Thr Pro Leu His Thr Ala 1590 1555 1600

GTA ATG AGT ACT TAT ATG GTT GTC GAT GCT TTG CTA AAT CAT CCA GAC 1S28 Val Met Ser Thr Tyr Met Val Val Asp Ala Leu Leu A≤n His Pro Asp 1505 1610 1615 1620

ATT GAT AAA AAT GCG CAG TCT ACG TCA GGA TTG ACT CCT TTC CAT TTA 13 5 lie Asp Lys Asn Ala Gin Ser Thr Ser Gly Lsu Thr Pro Phe His Leu 1625 1530 1635

GCA ATT ATT AAT GAA AGT CAA GAA GTT GCA GAA TCT TTA GTG GAA AGT 2024 Ala lie ie A≤n Glu Ser Gin Glu Val Ala Glu Ser Leu Val Glu Ser 1640 154.5 1650

AAT GCT GAT CTA AAT ATT CAG GAT GTT AAC CAT ATG GCT CCT ATT CAT 2072 Asn Ala Asp Lsu Asn lie G n Asp Val Asn His Met Ala Pro lie His 16=5 1660 1665

TTT GCA GCT TCA ATG GGT AGT ATT AAA ATG CTT AGA TAT CTC ATT TCC 2120 Phe Ala Ala Ser Met Gly S&r lie Lys Met Leu Arg Tyr L≥u He Ser 1570 1675 1530 .

2153 i e Lys Asp Lys Val S&r i e A≤n Ser Val Thr Gl u Asn Asn Asn Trp 1685 1690 1635 1700

ACA CCT TTA CAT TTT GCT ATA TAT TTT AAA AAA GAA GAT GCT GCA AAA 2216 Thr Pro Leu His Phe Ala ie Tyr Phe Lys Lys Glu Asp Ala Ala Lys 1705 1710 1715

GAA TTG TTG AAA CAA GAT GAC ATA AAT TTA ACA ATT GTT GCA GAT GGT 2254 Glu Lsu Leu Lys Gin Asp Asp ie Asn Leu Thr ie Val Ala Asp Gly 1720 1725 1730

AAT CTT ACC GTT TTA CAT CTT GCT GTT TCG ACA GGA CAA ATA AAT ATA 2312 Asn Leu Thr Val Leu His Leu Ala Val Ser Thr Gly Gin lie Asn lie 1735 1740 1745

ATT AAA GAA TTA TTG AAG AGA GGC TCC AAT ATA GAA GAA AAA ACT GGA 2360 lie Lys Glu Leu Leu Lys Arg Gly Ser A≤n lie Glu Glu Lys Thr Gly 1750 1755 1760

GAA GGA TAT ACA TCT CTC CAC ATC GCT GCG ATG CGA AAG GAG CCA GAG 2408 Glu Glv Tyr Tnr Ser Leu His lie Ala Ala Met Arg Lys Glu Pro Glu 17=5 ^" 1770 1775 1730

ATA GCT GTT GTT TTG ATT GAA AAC GGT GCT GAC ATA GAA GCT CGA TCA 2456 lie Ala Val Val Leu lie Glu Asn Gly Ala Asp χι _e Glu Ala Arg Ser -735 17S0 17=5

GCT GAT AAT TTA ACA CCT TTA CAT TCT GCC GCA AAA ATA GGA AGG AAA 2504 Ala Asp Asn Leu Thr Pro Leu His Arg Lys 1800

5UES7 SHEE ^{" •} ; i c ^r -r

r l

2552

2600

ATG AAA ACT GTT GAA ACT CTA TTA AAT AAA GGA GCA AAT TTA AAA GAA 2648 Met Lys Thr Val Glu Thr Leu Leu Asn Lys Gly Ala Asn Leu Lys Glu 1845 1850 1855 1860

TAC GAT AAC AAT AAA TAT TTG CCA ATA CAT AAA GCT ATT ATT AAT GAT 2696 Tyr Asp Asn Asn Lys Tyr Leu Pro lie His Lys Ala He lie Asn Asp 1865 1870 1875

GAC CTT GAC ATG GTA CGT TTG TTT CTT GAA AAA GAT CCC AGT CTC AAA 2744 Asp Leu Asp Met Val Arg Leu Phe Leu Glu Lys Asp Pro Ser Leu Lys 1880 1885 1890

GAT GAT GAA ACA GAA GAG GGT AGA ACT TCA ATT ATG TTA ATT GTT CAG 2792 Asp Asp Glu Thr Glu Glu Gly Arg Thr Ser lie Met Leu lie Val Gin 1895 1900 1905

AAA TTG CTT CTT GAA TTA TAT AAC TAT TTT ATA AAT AAT TAT GCT GAA 2840 Lys Leu Leu Leu Glu Leu Tyr Asn Tyr Phe lie Asn Asn Tyr Ala Glu 1910 1915 1920

ACT TTG GAT GAA GAA GCT TTA TTC AAC CGC TTA GAT GAA CAA GGG AAA 2888 Thr Leu Asp Glu Glu Ala Leu Phe Asn Arg Leu Asp Glu Gin Gly Lys 1925 1930 1935 1940

TTA GAG CTT GCA TAT ATC TTC CAT AAT AAA GAA GGT GAT GCA AAA GAG 2936 Leu Glu Leu Ala Tyr lie Phe His Asn Lys Glu Gly Asp Ala Lys Glu 1945 1950 1955

GCT GTT AAG CCA ACT ATC CTT GTT ACA ATT AAA CTT ATG GAA TAC TGC 2984 Ala Val Lys Pro Thr lie Leu Val Thr lie Lys Leu Met Glu Tyr Cys 1960 1965 1970

TTA AAA AAA CTT CGC GAA GAG TCT GGA GCT CCT GAA GGT AGT TTC GAT 3032 Leu Lys Lys Leu Arg Glu Glu Ser Gly Ala Pro Glu Gly Ser Phe Asp 1975 1980 1985

TCT CCA TCT TCA AAG CAA TGT ATT TCT ACC TTT TCA GAG GAT GAA ATG 3080 Ser Pro Ser Ser Lys Gin Cys lie Ser Thr Phe Ser Glu Asp Glu Met 1990 1995 2000

TTT CGT CGT ACT TTA CCG GAA ATT GTA AAA GAA ACG AAC AGC AGA TAT 3128 •Phe Arg Arg Thr Leu Pro Glu lie Val Lys Glu Thr Asn Ser Arg Tyr 2005 2010 2015 2020

TTA CCA CTA AAG GGC TTT TCT CGC AGC CTA AAT AAG TTT CTC CCT TCT 3176 Leu Pro Leu Lys Gly Phe Ser Arg Ser Leu Asn. Lys Phe Leu Pro Ser 2025 2030 2035

CTA AAA TTT GCC GAA AGT AAG AAT AGC TAC AGA TCT GAA AAT TTT GTT 3224 Leu Lys Phe Ala Glu Ser Lys Asn Ser Tyr Arg Ser Glu Asn Phe Val 2040 2045 2050

AGC AAT ATT GAT TCC AAC GGA GCA TTA CTT TTA CTC GAT GTA TTT ATC 3272 Ser Asn He Asp Ser Asn Gly Ala Leu Leu Leu Leu Asp Val Phe π _β 2055 2060 2065

AGA _A AG TTT ACT _AA T G _A G AAA TAC AAT TTG ACT GGA AAA GAA GCT GTA 3320 Arg Lys Phe Thr Asn Glu Lys Tyr Asn Lau Thr Gly Lys Glu Ala Val

SUBSTITUTE SHEET ⁽ RULE 25

- k~ - 2070 2075 2080

CCC TAT CTG GAA GCA AAG GCT TCA TCA TTA CGT ATC GCT TCT AAA TTT 3353

Pro Tyr Leu Glu Ala Lys Ala Ser Ser Leu Arg lie Ala Ser Lys Phe 20S5 2090 2095 2100

GAA GAA CTT CTA ACT GAA GTT AAA GGT ATT CCG GCT GGA GAG CTA ATT 3416

Glu Glu Leu Leu Thr Glu Val Lys Gly lie Pro Ala Gly Glu Leu lie 2105 2110 2115

AAT ATG GCC GAA GTG AGT TCC AAC ATA CAT AAG GCA ATT GCA AGT GGT 3464

Asn Met Ala Glu Val Ser Ser Asn lie His Lys Ala lie Ala Ser Gly 2120 2125 2130

AAG CCT GTA TCA AAA GTC TTA TGT TCG TAT TTG GAT ACC TTT TCT GAA 3512

Lys Pro Val Ser Lys Val Leu Cys Ser Tyr Leu Asp Thr Phe Ser Glu 2135 2140 2145

TTA AAT TCT CAA CAA ATG GAA GAA TTA GTT AAC ACA TAC TTA TCC ACC 3560

Leu Asn Ser Gin Gin Met Glu Glu Leu Val Asn Thr Tyr Leu Ser Thr 2150 2155 2160

AAA CCT TCT GTA ATT ACG TCA GCA TCT GCA GAT TAC CAG AAA CTT CCT 3608

Lys Pro Ser Val lie Thr Ser Ala Ser Ala Asp Tyr Gin Lys Leu Pro 21S5 2170 2175 2180

AAT TTG TTA ACT GCA ACT TGC TTA GAA CCA GAA AGA ATG GCT CAA CTT 3656

Asn Leu Leu Thr Ala Thr Cys Leu Glu Pro Glu Arg Met Ala Gin Leu 2185 2190 2195

ATA GAT GTG CAT CAA AAG ATG TTT TTA CGT TAAAATACCA TTCCTTCTGT 3706 lie Asp Val His Gin Lys Met Phe Leu Arg 2200 2205

(2) INFORMATION FOR SΞQ ID NO: 4:

Ci) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1214 am no acids CB) TYPE: amino acid CD) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SΞQ ID NO: 4:

Met His Ser Lys Glu Leu Gin Thr lie Ser Ala Ala Val Ala Arg Lys 1 5 10 15

Ala Val Pro Asn Thr Met Val He Arg Leu Lys Arg Asp Glu Glu Asp 20 25 30

Gly Glu Met Thr Leu Glu Glu Arg Gin Ala Gin Cys Lys Ala lie Glu

35 40 45

Tyr Ser Asn Ser Val Phe Gly Met lie Ala Asp Val Ala Asn Asp lie 50 55 60

Gly Ser lie Pro Val lie Gly Glu Val Val Gly lie Val Thr Ala Pro 65 70 75 80 lie Ala lie Val Ser His He Thr Ser Ala Gly Leu Asp He Ala Ser 85 90 95

Thr Ala Leu Asp Cys Asp Asp He Pro Phe Asp Glu lie Lys Glu π _e

TOO T05 no

- r ~

Leu Gl u Gl u Arg Phe Asn Gl u l i e Asp Arg Lys Leu A _{S D} Lvs Asn Thr U S 1 20 . ₂ l

Al a Al a Leu Gl u Gl u Val Ser Lys Leu Val Ser Lys Thr Phe Val Thr 130 135 140

Val Gl u Lys Thr Arg Asn Gl u Met Asn Gl u Asn Phe Lys Leu Val Leu 1 45 1 50 1 55 150

Gl u Thr H e Gl u Ser Lys Gl u H e Lys Ser H e Val Phe Lys H e Asn

165 170 ₁₇₅

Asp Phe Lys Gly Leu

Pro Lys Asp Arg Tyr Val Ala Lys Leu Leu Glu Gin Lys Gly He Leu 195 200 205

Gly Ser Leu Lys Glu Val Arg Glu Pro Ser Gly Asn Ser Leu Ser Ser 210 215 220

Ala Leu Asn Glu Leu Leu Asp Lys Asn Asn Asn Tyr Ala lie Pro Lys 225 230 235 240

Val Val Asp Asp Asn Lys Ala Phe Gin Ala Leu Tyr Ala Leu Phe Tyr 245 250 255

Gly Thr Gin Thr Tyr Ala Ala Val Met Phe Phe Leu Leu Glu Gin His 260 265 270

Ser Tyr Leu Ala Asp Tyr Tyr Tyr Gin uys Gly Asp Asp Val Asn Phe 275 280 285

Asn Ala Glu Phe Asn Asn Val Ala lie lie Phe Asp ASP Phe Lys Ser 290 295 300

Ser Leu Thr Gly Gly Asp Asp Gly Leu lie Asp Asn Val lie Glu Val 305 310 315 320

Leu Asn Thr Val Lys Ala Leu Pro Phe lie Lys Asn Ala Asp Ser Lys 325 330 335

Leu Tyr Arg Glu Leu Val Thr Arg Thr Lys Ala Leu Glu Thr Leu Lys

340 345 350

Asn Gin lie Lys Thr Thr Asp Leu Pro Leu lie Asp Asp lie Pro Glu 355 360 365

Thr Leu Ser Gin Val Asn Phe Pro Asn Asp Glu Asn Gin Leu Pro Thr

370 375 380

Pro lie Gly Asn Trp Val Asp Gly Val Glu Val Arg Tyr Ala Val Gin 385 390 395 400

Tyr Glu Ser Lys Gly Met Tyr Ser Lys Phe Ser Glu Trp Ser Glu Pro 405 410 415

Phe Thr Val Gin Gly Asn Ala Cys Pro Thr lie Lys Val Arg Val Asp

420 25 43C

Pro Lys Lys Arg Asn Arg Leu lie Phe Arg Lys Phe Asn Ser Gly Lys

435 440 445

Pro Gin Phe Ala Gly Thr Met T r His Ser Gin Thr Asr. Fne Lys Asn 5Q ^55 4=0

He His Arg Asp Leu Tyr Asp Ala Ala Leu Asn He Asn Lys Leu Lys 465 470 475 430

Ala Val Asp Glu Ala Thr Thr Leu He Glu Lys Gly Ala Asp He Glu 485 490 4g5

Ala Lys Phe Asp Asn Asp Arg Ser Ala Met His Ala Val Ala Tyr Arg 500 505 510

Gly Asn Asn Lys He Ala Leu Arg Phe Leu Leu Lys Asn Gin Ser He 515 520 525

Asp He Glu Leu Lys Asp Lys Asn Gly Phe Thr Pro Leu His He Ala 530 535 540

Ala Glu Ala Gly Gin Ala Gly Phe Val Lys Leu Leu He Asn His Gly 545 550 555 560

Ala Asp Val Asn Ala Lys Thr Ser Lys Thr Asn Leu Thr Pro Leu His 565 570 575

Leu Ala Thr Arg Ser Gly Phe Ser Lys Thr Val Arg Asn Leu Leu Glu 580 585 590

Ser Pro Asn He Lys Val Asn Glu Lys Glu Asp Asp Gly Phe Thr Pro 595 600 605

Leu His Thr Ala Val Met Ser Thr Tyr Met Val Val Asp Ala Leu Leu 610 615 620

Asn His Pro Asp He Asp Lys Asn Ala Gin Ser Thr Ser Gly Leu Thr 625 630 635 640

Pro Phe His Leu Ala He He Asn Glu Ser Gin Glu Val Ala Glu Ser 645 650 655

Leu Val Glu Ser Asn Ala Asp Leu Asn He Gin Asp Val Asn His Met 660 665 670

Ala Pro He His Phe Ala Ala Ser Met Gly Ser He Lys Met Leu Arg 675 680 685

Tyr Leu He Ser He Lys Asp Lys Val Ser He Asn Ser Val Thr Glu 690 695 700

Asn Asn Asn Trp Thr Pro Leu His Phe Ala He Tyr Phe Lys Lys Glu 705 710 715 720

Asp Ala Ala Lys Glu Leu Leu Lys Gin Asp Asp He Asn Leu Thr He 725 730 735

Val Ala Asp Gly Asn Leu Thr Val Leu His Leu Ala Val Ser Thr Gly 740 745 750

Gin He Asn He He Lys Glu Leu Leu Lys Arg Gly Ser Asn He Glu 755 760 765

Glu Lys Thr Gly Glu Gly Tyr Thr Ser Leu His He Ala Ala Met Arg 770 775 780

Lys Glu Pro Glu He Ala Val Val Leu He Glu Asn Gly Ala Asp He 735 790 795 800

Glu Ala Arg Ser Ala Asp Asn Leu Thr Pro Leu His Ser Ala Ala Lys 805 810 815

SϋSSXTJTo SHEET (RULE 26)

He Gly Arg Lys Ser Thr Val Leu Tyr Leu Leu Glu Lvs Glv Ala Asp 820 825 ^y _Q 30 lie Gly Ala Lys Thr Ala Asp Gly Ser Thr Ala Leu His Leu Ala Val 835 840 8 5

Ser Gly Arg Lys Met Lys Thr Val Glu Thr Leu Leu Asn Lys Gly Ala 850 855 860

Asn Leu Lys Glu Tyr Asp Asn Asn Lys Tyr Leu Pro He His Lys Ala 865 870 875 880 lie lie Asn Asp Asp Leu Asp Met Val Arg Leu Phe Leu Glu Lys Asp 885 890 395

Pro Ser Leu Lys Asp Asp Glu Thr Glu Glu Gly Arg Thr Ser He Met 900 905 giQ

Leu lie Val Gin Lys Leu Leu Leu Glu Leu Tyr Asn Tyr Phe He Asn 915 920 925

Asn Tyr Ala Glu Thr Leu Asp Glu Glu Ala Leu Phe Asn Arg Leu Asp 930 935 940

Glu Gin Gly Lys Leu Glu Leu Ala Tyr He Phe His Asn Lys Glu Gly

945 950 955 960

Asp Al Lys Glu Ala Val Lys Pro Thr He Leu Val T r He Lys Leu 965 970 975

Met Glu Tyr Cys Leu Lys Lys Leu Arg Glu Glu Ser Gly Ala Pro Glu 980 985 990

Gly Ser Phe Asp Ser Pro Ser Ser Lys Gin Cys He Ser Thr Phe Ser 995 1000 1005

Glu Asp Glu Met Phe Arg Arg Thr Leu Pro Glu! lie Val Lys Glu Thr 1010 1015 ^' 1020

Asn Ser Arg Tyr Leu Pro Leu Lys Gly Phe Ser Arg Ser Leu Asn Lys 1025 1030 1035 1040

Phe Leu Pro Ser Leu Lys Phe Ala Glu Ser Lys Asn Ser Tyr Arg Ser 1045 1050 1055

Glu Asn Phe Val Ser Asn He Asp Ser Asn Gly Ala Leu Leu Leu Leu 1060 1065 1070

Asp Val Phe He Arg Lys Phe Thr Asn Glu Lys Tyr Asn Leu Thr Gly 1075 1080 1085

Lys Glu Ala Val Pro Tyr Leu Glu Ala Lys Ala Ser Ser Leu Arg He 1090 1095 1100

Ala Ser Lys Phe Glu Glu Leu Leu Thr Glu Val Lys Gly He Pro Ala 1105 1110 1115 1120

Gly Glu Leu He Asn Met Ala Glu Val Ser Ser Asn He His Lys Ala 1125 1130 1135 lie Ala Ser Gly Lys Pro Val Ser Lys Val Leu Cys Ser Tyr Leu Aso 1140 1145 1150

Thr Phe Ser Glu Leu Asn Ser Gin Gin Met Glu Glu Leu Val Asn Thr 1155 1160 1165

Tyr Leu Ser Thr Lys Pro Ser Val lie Thr Ser Ala Ser Ala Asp Tyr 1170 1175 1180

Gin Lys Leu Pro Asn Leu Leu Thr Ala Thr Cys Leu Glu Pro Glu Arg 1185 1190 1195 120 ₍

Met Ala Gin Leu lie Asp Val His Gin Lys Met Phe Leu Ar _g

1205 1 21 0

SUBSTITUTE SHEET (RULE 26;