Title:
SPLIT-TYPE RECOMBINANT LUCIFERASE, AND ANALYSIS METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2016/038750
Kind Code:
A1
Abstract:
The present invention provides: a split-type recombinant protein; a gene encoding the split-type recombinant protein; and an analysis method using the split-type recombinant protein or the gene. The split-type recombinant protein comprises: an N-terminal-sided fragment of firefly luciferase, which is one of two split fragments of firefly luciferase that is split into two fragments so as to recover the firefly luciferase activity thereof when the two fragments are linked to each other; a C-terminal-sided fragment of firefly luciferase, which is the other of the two split fragments and also carries 58 to 78 amino acid residues that lie from a splitting position, at which the firefly luciferase can be split into the two fragments so as to recover the firefly luciferase activity thereof when the two fragments are linked to each other, toward the N-terminal of the firefly luciferase; and a linker peptide. In the split-type recombinant protein, the firefly luciferase activity can be recovered when the N-terminal-sided fragment and the C-terminal-sided fragment are linked to each other.
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Inventors:
SUGIYAMA TAKASHI (JP)
Application Number:
PCT/JP2014/074606
Publication Date:
March 17, 2016
Filing Date:
September 10, 2014
Export Citation:
Assignee:
OLYMPUS CORP (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12Q1/02; C12Q1/66
Foreign References:
| JP2011004734A | 2011-01-13 | |||
| JP2012051824A | 2012-03-15 | |||
| JP2011195547A | 2011-10-06 | |||
| JP2009296973A | 2009-12-24 | |||
| JP2011167079A | 2011-09-01 | |||
| JP2013074961A | 2013-04-25 | |||
| JP2013081459A | 2013-05-09 |
Other References:
LUKER,K.E. ET AL.: "Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals", PROC.NATL.ACAD.SCI. U.S.A., vol. 101, no. 33, 17 August 2004 (2004-08-17), pages 12288 - 93, XP002351606, DOI: doi:10.1073/pnas.0404041101
Attorney, Agent or Firm:
SUGIMURA, KENJI (JP)
Kenji Sugimura (JP)
Kenji Sugimura (JP)
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