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Patent Searching and Data


Title:
SRM ASSAYS TO CHEMOTHERAPY TARGETS
Document Type and Number:
WIPO Patent Application WO/2016/004233
Kind Code:
A3
Abstract:
Quantitative analysis of proteins that are targets of chemotherapy by Selected reaction monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry, by measuring modified forms of disclosed specific peptides from the ENT1, ERCC1, FOLR1, RRM1, TUBB3, TOP01, and/or TOP02A proteins. These peptides can be quantified if a modified form thereof comprises a phosphorylated, tyrosine, threonine, serine, and/or other amino acid residues within their amino acid sequence. Biological samples that have been fixed in formalin are used for the analysis.

Inventors:
KRIZMAN DAVID B (US)
HEMBROUGH TODD (US)
THYPARAMBIL SHEENO (US)
LIAO WEI-LI (US)
AN EUNKYUNG (US)
Application Number:
PCT/US2015/038874
Publication Date:
April 07, 2016
Filing Date:
July 01, 2015
Export Citation:
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Assignee:
EXPRESSION PATHOLOGY INC (US)
International Classes:
G01N31/00; G01N33/48; G01N33/50; H01J49/00
Foreign References:
US20100029005A12010-02-04
Other References:
REYES ET AL.: "The Equilibrative Nucleoside Transporter (ENT1) can be phosphorylated at multiple sites by PKC and PKA.", MOL MEMBR BIOL., vol. 28, no. 6, 2011, pages 412 - 26, XP055380593
DATABASE NCBI [o] 20 April 2014 (2014-04-20), "equilibrative nucleoside transporter 1 [Homo sapiens].", XP055380603, Database accession no. NP_001071643.1
"Liquid Tissue MS Protein Prep Kit for the isolation of proteins and peptides from formalin fixed tissue in preparation for mass spectrometry analysis.", EXPRESSION PATHOLOGY., 2009, pages 5, 6, Retrieved from the Internet [retrieved on 20151120]
MURATA ET AL.: "Human equilibrative nucleoside transporter 1 expression is a strong independent prognostic factor in UICC T3-T4 pancreatic cancer patients treated with preoperative gemcitabine-based chemoradiotherapy.", J HEPATOBILIARY PANCREAT SCI., vol. 19, no. 4, 2012, pages 413 - 425, XP035086569, DOI: doi:10.1007/s00534-011-0440-3
Attorney, Agent or Firm:
BOOTH, Paul, M. (1201 Third AvenueSuite 480, Seattle WA, US)
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