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Title:
STABILIZATION OF HYPOALLERGENIC, HYPERDIGESTIBLE PREVIOUSLY REDUCED PROTEINS
Document Type and Number:
WIPO Patent Application WO2002062370
Kind Code:
A3
Abstract:
Disulfide proteins showed mitigated allergenicity and increased digestibility by pepsin following reduction by thioredoxin. The sulfhydryl groups newly formed on reduction by thioredoxin (at 4 DEG C) or dithiothreitol (at 55 DEG C) were blocked with a physiological disulfide, such as cystamine or oxidized glutathione to obtain stable forms of the disarmed allergen. Allergenicity of the two derivatives was compared with that of the oxidized, reduced and reoxidized forms of BLG by skin testing dogs from a colony sensitized to cow's milk. The reoxidized form resembled the derivatives in having lower allergenicity. Stable forms of such disarmed, hypoallergenic and hyperdigestible disulfide protein allergens or just hypoallergenic or just hyperdigestible protein allergens are useful foods as well as clinical preparations.

Inventors:
BUCHANAN BOB B (US)
MORIGASAKI SUSUMU (US)
DEL VAL GREGORIO (US)
FRICK OSCAR L (US)
Application Number:
US0203936W
Publication Date:
November 14, 2002
Filing Date:
February 06, 2002
Export Citation:
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Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
BUCHANAN, BOB, B.
MORIGASAKI, SUSUMU
DEL VAL, GREGORIO
FRICK, OSCAR, L.
International Classes:
C12Q1/26; A21D2/26; A21D2/36; A21D8/04; A21D13/06; A23C9/12; A23C9/152; A23J1/12; A23J1/14; A23J3/08; A23J3/18; A23K1/16; A23L1/015; A23L1/16; A23L1/211; A23L1/305; A23L1/314; A61K35/20; A61K35/54; A61K38/00; A61K38/44; A61K39/35; A61K39/36; A61P37/00; A61P37/08; C07K1/113; C07K14/415; C07K14/435; C07K14/46; C07K14/76; C07K14/81; C12N9/02; C12N9/20; C12P21/02; C12Q1/34; A61K35/12; (IPC1-7): A61K38/44; A61K38/06; A61K31/198; A21D2/06; A61P37/08
Domestic Patent References:
WO1996012799A11996-05-02
WO1999020122A11999-04-29
Other References:
MORIGASAKI SUSUMU ET AL: "Stabilization of hypoallergenic, hyperdigestible forms of the milk allergen beta-lactoglobulin.", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 107, no. 2, February 2001 (2001-02-01), 57th Annual Meeting of the American Academy of Allergy, Asthma and Immunology;New Orleans, Louisiana, USA; March 16-21, 2001, pages S138, XP001098620, ISSN: 0091-6749
BUCHANAN B B ET AL: "Thioredoxin: A multifunctional regulatory protein with a bright future in technology and medicine", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, NEW YORK, US, US, vol. 314, no. 2, 1 November 1994 (1994-11-01), pages 257 - 260, XP002089742, ISSN: 0003-9861
BUCHANAN B B ET AL: "Thioredoxin-linked mitigation of allergic responses to wheat", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 94, no. 10, 1 May 1997 (1997-05-01), pages 5372 - 5377, XP002089741, ISSN: 0027-8424
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