Title:
Stabilized ribozyme analogs
Document Type and Number:
WIPO Patent Application WO1996019568
Kind Code:
A3
Abstract:
Disclosed are stabilized ribozyme analogs having the ability to endonucleolytically cleave a sequence of 3' to 5' linked ribonucleotides. These ribozyme analogs include modifications at specific loop, catalytic core, and flanking region nucleotides which makes them more resistant to nucleases. Also disclosed are methods of preparing and utilizing the ribozyme analogs of the invention, and pharmaceutical formulations and kits containing such ribozyme analogs.
Inventors:
GOODCHILD JOHN (US)
NESBITT STEVEN M (US)
NESBITT STEVEN M (US)
Application Number:
PCT/US1995/015871
Publication Date:
September 06, 1996
Filing Date:
December 05, 1995
Export Citation:
Assignee:
HYBRIDON INC (US)
International Classes:
C12N15/09; C07H21/02; C12N15/113; (IPC1-7): C12N9/00; A61K31/70; C07H21/02
Domestic Patent References:
| WO1994013688A1 | 1994-06-23 | |||
| WO1993023569A1 | 1993-11-25 | |||
| WO1993015187A1 | 1993-08-05 |
Other References:
USMAN, N. ET AL.: "CHEMICAL MODIFICATION OF HAMMERHEAD RIBOZYMES: ACTIVITY AND NUCLEASE RESISTANCE", NUCLEIC ACIDS RESEARCH SYMPOSIUM SERIES, no. 31, 9 November 1994 (1994-11-09) - 11 November 1994 (1994-11-11), pages 163/164, XP002002020
GOODCHILD, J.: "Enhancement of ribozyme catalytic activity by a contiguous oligodeoxynucleotide (facilitator) and by 2'-O-methylation.", NUCLEIC ACIDS RESEARCH, (1992 SEP 11) 20 (17) 4607-12, XP002004870
PAOLELLA, G. ET AL.: "NUCLEASE RESISTANT RIBOZYMES WITH HIGH CATALYTIC ACTIVITY", EMBO JOURNAL, vol. 11, no. 5, 1 May 1992 (1992-05-01), pages 1913 - 1919, XP000268586
GOODCHILD, J.: "Enhancement of ribozyme catalytic activity by a contiguous oligodeoxynucleotide (facilitator) and by 2'-O-methylation.", NUCLEIC ACIDS RESEARCH, (1992 SEP 11) 20 (17) 4607-12, XP002004870
PAOLELLA, G. ET AL.: "NUCLEASE RESISTANT RIBOZYMES WITH HIGH CATALYTIC ACTIVITY", EMBO JOURNAL, vol. 11, no. 5, 1 May 1992 (1992-05-01), pages 1913 - 1919, XP000268586
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