Title:
STABLE CELL LINES RESISTANT TO APOPTOSIS AND NUTRIENT STRESS AND METHODS OF MAKING SAME
Document Type and Number:
WIPO Patent Application WO2001066696
Kind Code:
A3
Abstract:
Cell lines that are commonly used for protein expression are engineered to include genes that emcode suppressors of apoptosis (SA). Insect cell lines expressing these SA genes are resistant to apoptosis or programmed cell death, and express certain recombinant proteins at increased levels. Theses cell lines also have increased resistance to many types of stress. Because some of the SA proteins inhibit apoptosis in a wide spectrum of organisms, these genes may be inserted into other plant or animal cell lines for a variety of purposes involving resistance to apoptosis or resistance to stress.
Inventors:
BLISSARD GARY A (US)
GRANADOS ROBERT R (US)
GRANADOS ROBERT R (US)
Application Number:
PCT/US2001/006846
Publication Date:
March 14, 2002
Filing Date:
March 02, 2001
Export Citation:
Assignee:
THOMPSON BOYCE PLANT RES (US)
BLISSARD GARY A (US)
GRANADOS ROBERT R (US)
BLISSARD GARY A (US)
GRANADOS ROBERT R (US)
International Classes:
C07K14/195; C07K14/47; C12N5/07; C12N15/866; (IPC1-7): C12N5/10; C12N5/06; C07K14/01; C12N15/85; C12N15/86; C12N15/67
Domestic Patent References:
| WO2001023592A2 | 2001-04-05 | |||
| WO1999015677A1 | 1999-04-01 |
Other References:
MASTRANGELO A J ET AL: "Overcoming apoptosis: new methods for improving protein-expression systems", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 16, no. 2, 1 February 1998 (1998-02-01), pages 88 - 95, XP004107048, ISSN: 0167-7799
AL-RUBEAI MOHAMED ET AL: "Apoptosis in cell culture.", CURRENT OPINION IN BIOTECHNOLOGY, vol. 9, no. 2, April 1998 (1998-04-01), pages 152 - 156, XP002183264, ISSN: 0958-1669
MITCHELL-LOGEAN CHRISTINE ET AL: "bcl-2 Expression in Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 insect cells: Effect on recombinant protein expression and cell viability", BIOTECHNOL BIOENG;BIOTECHNOLOGY AND BIOENGINEERING NOV 20 1997 JOHN WILEY & SONS INC, NEW YORK, NY, USA, vol. 56, no. 4, 20 November 1997 (1997-11-20), pages 380 - 390, XP002183265
DATABASE WPI Section Ch Week 199735, Derwent World Patents Index; Class B04, AN 1997-380167, XP002183266
SUZUKI E ET AL: "ESTABLISHING APOPTOSIS RESISTANT CELL LINES FOR IMPROVING PROTEIN PRODUCTIVITY OF CELL CULTURE", CYTOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, vol. 23, no. 1-3, 1997, pages 55 - 59, XP001009825, ISSN: 0920-9069
CARTIER JL ET AL: "Suppression of apoptosis in insect cells stably transfected with baculovirus p35: dominant interference by N-terminal sequences p35(1-76)", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 68, no. 12, 1 December 1994 (1994-12-01), pages 7728 - 7737, XP002091607, ISSN: 0022-538X
AL-RUBEAI MOHAMED ET AL: "Apoptosis in cell culture.", CURRENT OPINION IN BIOTECHNOLOGY, vol. 9, no. 2, April 1998 (1998-04-01), pages 152 - 156, XP002183264, ISSN: 0958-1669
MITCHELL-LOGEAN CHRISTINE ET AL: "bcl-2 Expression in Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 insect cells: Effect on recombinant protein expression and cell viability", BIOTECHNOL BIOENG;BIOTECHNOLOGY AND BIOENGINEERING NOV 20 1997 JOHN WILEY & SONS INC, NEW YORK, NY, USA, vol. 56, no. 4, 20 November 1997 (1997-11-20), pages 380 - 390, XP002183265
DATABASE WPI Section Ch Week 199735, Derwent World Patents Index; Class B04, AN 1997-380167, XP002183266
SUZUKI E ET AL: "ESTABLISHING APOPTOSIS RESISTANT CELL LINES FOR IMPROVING PROTEIN PRODUCTIVITY OF CELL CULTURE", CYTOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, vol. 23, no. 1-3, 1997, pages 55 - 59, XP001009825, ISSN: 0920-9069
CARTIER JL ET AL: "Suppression of apoptosis in insect cells stably transfected with baculovirus p35: dominant interference by N-terminal sequences p35(1-76)", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 68, no. 12, 1 December 1994 (1994-12-01), pages 7728 - 7737, XP002091607, ISSN: 0022-538X
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