Title:
STEM CELL CLASSIFICATION METHOD AND APPARATUS USING RAMAN SPECTROSCOPIC ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2023/136510
Kind Code:
A1
Abstract:
Provided are a stem cell classification method and apparatus using Raman spectroscopic analysis. A stem cell classification method, according to one embodiment of the present invention, is a method for classifying stem cells by using a Raman spectroscopic inspection module and a classification device, the method comprising the steps of: preparing and pre-processing a specimen containing stem cells; comparing and analyzing Raman spectroscopy spectrum images through Raman spectroscopy; comparing a result of the Raman spectroscopy with a Raman standard average value for evaluating the direction and state of the differentiation of the stem cells; classifying the differentiation and growth types of the stem cells; and quantifying the differentiation and growth types of the stem cells, by including the Raman standard average value.
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Inventors:
KIM JUNKI (KR)
JUE MIYEON (KR)
JUE MIYEON (KR)
Application Number:
PCT/KR2022/021432
Publication Date:
July 20, 2023
Filing Date:
December 27, 2022
Export Citation:
Assignee:
ASAN FOUND (KR)
UNIV ULSAN FOUND IND COOP (KR)
UNIV ULSAN FOUND IND COOP (KR)
International Classes:
G01N21/65; G01J3/44; G01N33/483; G06N20/00
Foreign References:
| KR20200068697A | 2020-06-15 | |||
| KR20190054746A | 2019-05-22 |
Other References:
AKAGI YUKA, MORI NOBUHITO, KAWAMURA TERUHISA, TAKAYAMA YUZO, KIDA YASUYUKI S.: "Non-invasive cell classification using the Paint Raman Express Spectroscopy System (PRESS)", SCIENTIFIC REPORTS, vol. 11, no. 1, XP093078334, DOI: 10.1038/s41598-021-88056-3
HSU CHIA-CHEN, XU JIABAO, BRINKHOF BAS, WANG HUI, CUI ZHANFENG, HUANG WEI E., YE HUA: "A single-cell Raman-based platform to identify developmental stages of human pluripotent stem cell-derived neurons", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 117, no. 31, 4 August 2020 (2020-08-04), pages 18412 - 18423, XP093078335, ISSN: 0027-8424, DOI: 10.1073/pnas.2001906117
GENG JUNNAN, ZHANG WEI, CHEN CHENG, ZHANG HAN, ZHOU ANHONG, HUANG YU: "Tracking the Differentiation Status of Human Neural Stem Cells through Label-Free Raman Spectroscopy and Machine Learning-Based Analysis", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 93, no. 30, 3 August 2021 (2021-08-03), US , pages 10453 - 10461, XP093078336, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.0c04941
HSU CHIA-CHEN, XU JIABAO, BRINKHOF BAS, WANG HUI, CUI ZHANFENG, HUANG WEI E., YE HUA: "A single-cell Raman-based platform to identify developmental stages of human pluripotent stem cell-derived neurons", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 117, no. 31, 4 August 2020 (2020-08-04), pages 18412 - 18423, XP093078335, ISSN: 0027-8424, DOI: 10.1073/pnas.2001906117
GENG JUNNAN, ZHANG WEI, CHEN CHENG, ZHANG HAN, ZHOU ANHONG, HUANG YU: "Tracking the Differentiation Status of Human Neural Stem Cells through Label-Free Raman Spectroscopy and Machine Learning-Based Analysis", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 93, no. 30, 3 August 2021 (2021-08-03), US , pages 10453 - 10461, XP093078336, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.0c04941
Attorney, Agent or Firm:
BLT PATENT & LAW FIRM (KR)
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