Title:
STEM CELL CULTURE METHOD
Document Type and Number:
WIPO Patent Application WO/2014/027176
Kind Code:
A3
Abstract:
Methods for culturing pluripotent stem cells on fiber scaffolds are provided which result in the expansion of the number of stem cells without loss of pluripotency. Cells obtained by such methods, implants containing such cells and medical methods using such cells are also disclosed.
Inventors:
YANG YING (GB)
KUMAR DEEPAK (GB)
FORSYTH NICHOLAS (GB)
KUMAR DEEPAK (GB)
FORSYTH NICHOLAS (GB)
Application Number:
PCT/GB2013/051902
Publication Date:
May 08, 2014
Filing Date:
July 16, 2013
Export Citation:
Assignee:
UNIV KEELE (GB)
International Classes:
A61L27/38; C12N5/073
Domestic Patent References:
| WO2011142832A2 | 2011-11-17 | |||
| WO2010059778A1 | 2010-05-27 |
Foreign References:
| US20110033928A1 | 2011-02-10 | |||
| US20090269845A1 | 2009-10-29 |
Other References:
GAUTHAMAN KALAMEGAM ET AL: "Nanofibrous substrates support colony formation and maintain stemness of human embryonic stem cells", JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, vol. 13, no. 9B, September 2009 (2009-09-01), pages 3475 - 3484, XP002721382, ISSN: 1582-1838
IAN WIMPENNY ET AL: "One-Step Recovery of Marrow Stromal Cells on Nanofibers", TISSUE ENGINEERING PART C: METHODS, vol. 16, no. 3, 1 June 2010 (2010-06-01), pages 503 - 509, XP055106002, ISSN: 1937-3384, DOI: 10.1089/ten.tec.2009.0128
LI LIU ET AL: "Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions", BIOTECHNOLOGY LETTERS, SPRINGER NETHERLANDS, DORDRECHT, vol. 34, no. 10, 20 June 2012 (2012-06-20), pages 1951 - 1957, XP035131452, ISSN: 1573-6776, DOI: 10.1007/S10529-012-0973-9
IAN WIMPENNY ET AL: "One-Step Recovery of Marrow Stromal Cells on Nanofibers", TISSUE ENGINEERING PART C: METHODS, vol. 16, no. 3, 1 June 2010 (2010-06-01), pages 503 - 509, XP055106002, ISSN: 1937-3384, DOI: 10.1089/ten.tec.2009.0128
LI LIU ET AL: "Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions", BIOTECHNOLOGY LETTERS, SPRINGER NETHERLANDS, DORDRECHT, vol. 34, no. 10, 20 June 2012 (2012-06-20), pages 1951 - 1957, XP035131452, ISSN: 1573-6776, DOI: 10.1007/S10529-012-0973-9
Attorney, Agent or Firm:
SALISBURY, Frances et al. (33 Gutter LaneLondon, Greater London EC2V 8AS, GB)
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