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Title:
STRAIN AND METHOD FOR PRODUCING ROSMARINIC ACID
Document Type and Number:
WIPO Patent Application WO/2020/258896
Kind Code:
A1
Abstract:
Provided are a strain and method for producing rosmarinic acid. Recombinant cells expressing 4-coumarate-CoA ligase, rosmarinic acid synthase, polyphosphate kinase 2-I, and polyphosphate kinase 2-II or combinations of the recombinant cells are constructed, and the recombinant cells or the combinations of the recombinant cells are used for catalyzing synthesis of rosmarinic acid from Danshensu and caffeic acid.

Inventors:
CAI YUJIE (CN)
YAN YI (CN)
DING YANRUI (CN)
BAI YAJUN (CN)
ZHENG XIAOHUI (CN)
Application Number:
PCT/CN2020/076363
Publication Date:
December 30, 2020
Filing Date:
February 24, 2020
Export Citation:
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Assignee:
HONGTAOSIM RESEARCH INSTITUTE OF ANALYCAL SCIENCE AND TECH LTD CO (CN)
International Classes:
C12P7/62; C12N15/64; C12R1/19
Foreign References:
CN109880862A2019-06-14
CN102061301A2011-05-18
CN102876713A2013-01-16
US20060172402A12006-08-03
CN103834685A2014-06-04
US20130315983A12013-11-28
CN108658769A2018-10-16
CN107935855A2018-04-20
Other References:
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ZHUANG, YIBIN ET AL.: "Synthesis of Rosmarinic Acid Analogues in Escherichia Coli", BIOTECHNOLOGY LETTERS, vol. 38, no. 4, 14 December 2015 (2015-12-14), pages 619 - 627, XP035648217, ISSN: 1573-6776
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"Reference for determining the activity of 4-coumarate:CoA ligase: 4-Coumarate: CoA ligase from cell suspension cultures of Petroselinum hortense Hoffm", ARCH. BIOCHEM. BIOPHYS., vol. 184, 1977, pages 237 - 248
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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