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Title:
SUBSTITUTED UNSYMMETRICAL CYANINE DYES WITH SELECTED PERMEABILITY
Document Type and Number:
WIPO Patent Application WO1996013552
Kind Code:
A3
Abstract:
The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a pyridinium or quinolinium ring system. The cyanine dyes of the invention possess a high sensitivity to oligonucleotides and larger nucleic acid polymers in a wide range of cells and gels, and are useful for the analysis of cell structure, membrane integrity or function, and determination of cell cycle distribution. 00000

Inventors:
YUE STEPHEN T
SINGER VICTORIA L
ROTH BRUCE L
MOZER THOMAS J
MILLARD PAUL J
JONES LAURIE J
JIN XIAOKUI
HAUGLAND RICHARD P
POOT MARTIN
Application Number:
US9513706W
Publication Date:
July 11, 1996
Filing Date:
October 27, 1995
Export Citation:
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Assignee:
MOLECULAR PROBES, INC.
International Classes:
G01N33/48; C07D215/18; C07D413/06; C07D417/06; C07D417/14; C09B23/00; C09B23/02; C12Q1/68; G01N21/76; G01N33/58; (IPC1-7): C09B23/02
Domestic Patent References:
WO1994024213A11994-10-27
WO1993006482A11993-04-01
Foreign References:
US5321130A1994-06-14
DE2135413A11972-01-20
US4665024A1987-05-12
EP0226272A11987-06-24
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