Title:
A SUBSTRATE AND A METHOD FOR DETERMINING AND/OR MONITORING ELECTROPHYSIOLOGICAL PROPERTIES OF ION CHANNELS
Document Type and Number:
WIPO Patent Application WO2001025769
Kind Code:
A3
Abstract:
The present invention relates to a substrate and a method for obtaining an electrophysiological measuring configuration in which a cell forms a high resistive seal (giga-seal) around a measuring electrode making it suitable for determining and monitoring a current flow through the cell membrane. The substrate is typically part of an apparatus for studying electrical events in cell membranes, such as an apparatus for carrying out patch clamp techniques utilised to study ion transfer channels in biological membranes. The substrate has a plurality or an array of measuring sites with integrated measuring and reference electrodes formed by wafer processing technology. The electrodes are adapted to conduct a current between them by delivery of ions by one electrode and receipt of ions by the other electrode and are typically silver/silver halide electrodes. This allows for effective and fast measuring of cells in configurations where there is a direct electrical connection between the measuring electrode and the cell interior, a whole-cell measuring configuration.
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Inventors:
PETERSEN JON WULFF
TELLEMAN PIETER
HANSEN OLE
CHRISTOPHERSEN PALLE
BECH MORTEN
OLESEN SOEREN PETER
DUE JOERGEN
THOMSEN LARS
TELLEMAN PIETER
HANSEN OLE
CHRISTOPHERSEN PALLE
BECH MORTEN
OLESEN SOEREN PETER
DUE JOERGEN
THOMSEN LARS
Application Number:
PCT/DK2000/000548
Publication Date:
August 30, 2001
Filing Date:
October 02, 2000
Export Citation:
Assignee:
SOPHION BIOSCIENCE AS (DK)
International Classes:
C12M1/34; G01N27/04; G01N33/483; G01N27/22; G01N27/30; G01N27/416; G01N27/447; G01N33/487; (IPC1-7): G01N33/483; G01N27/02
Foreign References:
| EP0299778A2 | 1989-01-18 | |||
| US4225410A | 1980-09-30 | |||
| US4062750A | 1977-12-13 | |||
| EP0299779A2 | 1989-01-18 |
Other References:
M. MULLENBORN ET AL: "Fast 3d Laser Micromachining of Silicon for Micromechanical and Microfluid Applications", SOLID-STATE SENSORS AND ACTUATORS, 1995 AND EUROSENSORS IX.. TRANSDUCERS '95. THE 8TH INTERNATIONAL CONFERENCE ON,, STOCKHOLM, SE, XP002901487
WEAVER J C: "Electroporation of cells and tissues", IEEE TRANSACTIONS ON PLASMA SCIENCE, vol. 28, no. 1, February 2000 (2000-02-01), pages 24 - 33, XP002901488
WEAVER J C: "Electroporation of cells and tissues", IEEE TRANSACTIONS ON PLASMA SCIENCE, vol. 28, no. 1, February 2000 (2000-02-01), pages 24 - 33, XP002901488
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