Title:
SULFOHYDROLASES, CORRESPONDING AMINO ACID AND NUCLEOTIDE SEQUENCES, SULFOHYDROLASE PREPARATIONS, PROCESSES, AND PRODUCTS THEREOF
Document Type and Number:
WIPO Patent Application WO2000068395
Kind Code:
A3
Abstract:
A purified sulfohydrolase having a purity level based on total amount of protein of at least about 40 wt.%. Isolated nucleic acid sequence and amino acid sequences. A process for purifying at least one sulfohydrolase, including subjecting an extract from seaweed to fractionation to obtain fractions; and subjecting at least one of the fractions to phenyl sepharose chromatography to obtain sepharose fractions containing at least one sulfohydrolase. An enzymatically modified compound which has been modified by an isolated sulfohydrolase having a purity level based on total amount of protein of at least about 40 wt.%. A process of enzymatically modifying a sulfated compound, including combining at least one sulfohydrolase, having a purity level based on total amount of protein of at least about 40 wt.%, with a sulfated compound form a reaction mixture; and incubating the reaction mixture to remove sulfate groups from the sulfated compound to form an enzymatically modified compound. A process of enzymatically modifying a sulfated compound, including incubating a first sulfohydrolase with a sulfated compound to remove sulfate groups from the sulfated compound to form an intermediate compound; and subsequently incubating the intermediate compound with a second sulfohydrolase to remove sulfate groups to form an enzymatically modified compound. A method for extracting one of nu- and mu-carrageenan from seaweed, including dispersing seaweed in a salt solution including K2CO3 to form a dispersion; filtering the dispersion to obtain a liquid; ultrafiltering the dispersion to remove salts; concentrating the liquid; adjusting the pH of the liquid to about 8 to 8.5; and precipitating one of nu- and mu-carrageenan from the liquid.
Inventors:
GENICOT SABINE
DE RUITER GERHARD
KLOAREG BERNARD
PENNINKHOF BEA
POTIN PHILLIPE
RICHARD ODILE
RUDOLPH BRIAN
DE RUITER GERHARD
KLOAREG BERNARD
PENNINKHOF BEA
POTIN PHILLIPE
RICHARD ODILE
RUDOLPH BRIAN
Application Number:
PCT/US2000/012531
Publication Date:
March 08, 2001
Filing Date:
May 09, 2000
Export Citation:
Assignee:
HERCULES INC (US)
International Classes:
C08B37/00; C08B37/12; C12N9/14; C12N15/09; C12N9/16; C12N15/55; C12P19/04; (IPC1-7): C12N15/55; C12N9/14; C08B37/00
Other References:
CRAIGIE ET AL.: "Carrageenan biosynthesis", PROC INT SEAWEED SYMP, 1978, pages 369 - 377, XP000964560
WONGET AL.: "Sulfohydrolase activity and carrageenan biosynthesis in Chondrus crispus (Rhodophyceae)", PLANT PHYSIOLOGY, vol. 61, no. 4, 1978, pages 663 - 666, XP000961463
MCLEAN ET AL.: "Neocarratetraose 4-O-monosulphate beta-hydrolase from Pseudomonas carrageenovora", EUR J BIOCHEM, vol. 113, 1981, pages 447 - 456, XP000964501
SHAW ET AL.: "Substrate specificity and other prperties of the inducible S3 secondary alkylsulphohydrolase ....", BIOCHEM J, vol. 187, 1980, pages 181 - 196, XP000961652
WONGET AL.: "Sulfohydrolase activity and carrageenan biosynthesis in Chondrus crispus (Rhodophyceae)", PLANT PHYSIOLOGY, vol. 61, no. 4, 1978, pages 663 - 666, XP000961463
MCLEAN ET AL.: "Neocarratetraose 4-O-monosulphate beta-hydrolase from Pseudomonas carrageenovora", EUR J BIOCHEM, vol. 113, 1981, pages 447 - 456, XP000964501
SHAW ET AL.: "Substrate specificity and other prperties of the inducible S3 secondary alkylsulphohydrolase ....", BIOCHEM J, vol. 187, 1980, pages 181 - 196, XP000961652
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