SUPER CRITICALLY ENABLED MOLECULAR DISPERSION (TRUE SOLUTION) OF LIPOPHILIC BIOACTIVE IN LIPID MATRIX AND CORRESPONDING METHOD THEREOF Field of Invention Present invention describes a composition and a method of its preparation comprising of a lipid matrix in which lipophilic bioactive is dispersed at a molecular level. Such molecular dispersion is made possible through supercritical carbon dioxide as a solvent and a food or pharma grade oil as a lipid matrix. Lipophilic bioactive, as per this invention, includes medicinal agents, vitamins and nutrients for human and animal use. Particularly, this invention describes a molecular dispersion of lipophilic nutrients such as carotenoids, curcumin, ginger, omega 3 fatty acids, tocopherols, tocotrienols, vitamin A, K and the like. More particularly, this invention describes a molecular dispersion of lipophilic nutrients in an oily matrix such as sunflower oil, safflower oil, soybean oil, corn oil, canola oil, castor oil, ground nut oil and the like. Dispersion of lipophilic nutrients in an oily matrix is enabled through supercritical carbon dioxide as a solvent under certain conditions of pressure, temperature and duration of mixing. Molecular dispersion of bioactive may further be stabilized by the addition of a stabilizer and additives which will enable homogenous dispersion. The Molecular dispersions of lipophilic nutrients in a lipid matrix have applications in enhancing the absorption and bioavailability. Molecular dispersions of lipophilic nutrients in lipid matrix also stabilizes the lipophilic nutrient by protecting them from oxidation, stomach acid, digestive enzymes, and microsomal enzymes. At a molecular level, by using certain lipid matrix, it is possible to enhance lymphatic absorption and minimize portal absorption. Background of the Invention The plant bioactives particularly carotenoids are poorly soluble in lipophilic carriers such as oils. To improve the Bio-delivery of plant derived bioactives several strategies have been attempted by different Researchers. In this the aqueous solubility of the nutraceutical bioactive is important and will determine the bioavailability and availability of bioactive for systemic absorption via biological membranes. This makes the requirement of solubility of the nutraceutical drug substance is major concern for formulation. As discussed, the drug substances including bioactive have the bioavailability problem mainly due to the low aqueous solubility and the rate limiting step is the dissolution process. Therefore, for improvising the solubility in lipid medium and to some extent in aqueous medium, the selection of proper additives for targeted delivery of bioactive is very decisive to overcome the major obstruction in bioavailability and absorption. The molecular dispersion of a bioactive is an emerging field and could be achieved through particle design through different techniques including SCF-CO2. The specially designed SCF-CO2 equipment could be applied for particle designing which are very cost intensive. However, in the present invention the focus is to achieve the target through the SCF-CO2 extraction vessel, designed for high pressure extraction. The design modification done to disperse the bioactive molecules at high pressure and optimized temperature leads to the dissolution/dispersion of bioactive molecule. The dispersed bioactive in lipid matrix will result in higher bioaccessability thereby improving the bioavailability of the bioactive nutraceutical compounds as the higher amount of bioactive will be made available in the gastro- intestinal lumen for absorption in the intestine. For achieving the carotenoids bioaccessability and therefore, the higher concentration for intestinal absorption, the bioactive carotenoids need to be released from its food matrix and incorporated and dissolved into a hydrophobic medium (bulk lipid emulsion, or mixed micelles). A number of studies conducted in the past indicate that the carotenoids dissolved in oil are more bioavailable than carotenoids that come directly from their food source. Therefore, we aim to use vegetable oil as a lipid medium for making the bioactive more bioavailable with the use of SCF-CO2. The aim of the current invention is to further improvise the dispersion/solution of bioactives with vegetable oil and making the submicron particle with SCF-CO2. The lipid matrix prepared in such a way with green solvent in to a molecular dispersion will lead to digestion in the GIT and supporting intestinal lymphatic transport. This process may lead to bioactive supersaturation, leading to increased absorption. Therefore, the aim of the present invention is not only to improve the bioavailability of the bio-actives but also to make it amenable to bio- delivery. This is aimed to target the lymphatic absorption of the bioactive and bypass the first pass metabolism. Conventionally to improvise the bioavailability of the bioactives which are mainly poorly water-soluble lipophilic compounds, the process of improving solubility/bioavailability through high-speed homogenization, homogenization in oils, milling and salt formation is attempted. The colloidal milling or ball milling are again may result in stress impacting the stability of bioactive compounds. As discussed, the conventional method of molecular dispersion achieved with high pressure homogenization, ball milling and colloidal milling will result in reduction in the size of bioactive compound in micron and sub-micron level with increased surface area, which will ultimately lead to better and faster diffusion/ absorption. However, the process of homogenization will have limitation for thermos-labile carotenoids and xanthophyll’s as the physical stress may cause thermal degradation. In addition, controlling shape, size and morphology of the particles is also not easy. Another limitation of the process is the use of high concentration of surfactants and co- surfactants to make the lipid dispersion. This will further make the process less charming as in addition to the high concentration of surfactants it will need high pressure homogenization which will be capital intensive and energy intensive. Another process employed for making the submicron particle dispersion is using lauroyl macroglycerides and polyethylene glycols ester. However, these processes also require high quantity of the ingredients making the process less cost-competitive and high concentration of surfactants may have adverse impact on the consumer. The process of salt formation of plant derived bioactives is another method to improve bioavailability. However, this process suffers from the effect like epigastric distress due to the high alkalinity, reactivity with atmospheric water and atmospheric gases particularly carbon dioxide. In the similar lines to the salt formation, adjusting pH to convert a water insoluble bioactive to make better water soluble could be useful. However, due to extreme pH condition stability is major issue. All above techniques are mentioned here to have a preliminary idea of making oil solution of nutraceutical compounds. However, we focus on the application of supercritical fluid carbon dioxide for preparing oil solution. To achieve the oil solution of bioactive nutraceuticals, lipid bioactive was run in the SCF- CO2 with food grade vegetable oil ranging from sunflower, soya, canola, ground nut, coconut, palm oil and olive oil at pressure ranging from 110-900 bar. The aim of particle design of the plant bioactive particularly carotenoids and xanthophyll was to achieve the lipid matrix which is dispersed and have better bio-delivery to the targeted organs. The process of preparing the improved bioavailable oil solution with vegetable oils and with the application of SCF-CO2 is described as SCEMOD Super Critically Enabled Molecular Dispersion. The technology as named for the process of preparing molecular dispersion of the bioactive compounds in vegetable oils and making the use of high pressure SCF-CO2 for enabling molecular dispersion. The oil used for extraction/ dispersion/dilution media may additionally contain stabilizers which stabilizes the molecular dispersion. The SCF-CO2 extraction/enrichment is attempted in two stage process. Extraction of plant bioactive from the plant source followed by mixing with oils and natural stabilizers. The oil used for dilution is 0.5-1.5 Volume of the SCF-CO2 extract and generally added as per the strength of bioactive (content & mass), phospholipids and polyethylene glycol and may also contain 0.1-5% Polysorbate 80. The process was done in the SCF-CO2 by adding the vegetable oil to the plant bioactive, i.e., lutein ester, Astaxanthin and or turmeric extract, thereafter, conducting SCF-CO2 homogenization /extraction at 125-900 bar and temperature varying from 40-70 degree Celsius with Co2 flow rate of 0.5-2.0 Kg/min. The process further led to the removal of waxes and resins along with the water from the marigold meal by a two-stage extraction, i. e. at 150-300 bar and 50-70 degree Celsius followed by extraction at 450-900 bar and temperature 40- 70 degree Celsius. For the extraction and particle of botanical extract with the vegetable oils SCF-CO2 led to the molecular dispersion. The lipid matrix contains homogenous sub- micron particles which result in improved dispersion. The targeted molecular dispersion with different vegetable oil particularly olive oil is aimed to make the bioactive more bioavailable in the similar lines with the study conducted by Food research foundation on lutein, which showed that when fed with different oils, the mice fed with olive oil gave the highest absorption rate lutein in the intestinal tract. To prepare the delivery of Lipid-based oral drug for poorly soluble lipophilic bioactives is gaining a lot of focus in recent times (Pouton, 2006; Chakraborty et al., 2009). The unique properties of lipids, namely, their physicochemical diversity, biocompatibility, and ability to enhance oral bioavailability of poorly water-soluble lipophilic drugs through selective lymphatic uptake have made them attractive candidates as carriers for oral formulations. Among these the molecular dispersion is one of the promising approach to improve the solubility and absorption of poorly water soluble lipophilic bioactive, (Shao et al., 2010; Wu et al., 2011; Ma et al., 2012; Vithlani et al., 2012). The bioactive delivery as reported by Yonekura and Nagao, 2007 showed xanthophyll’s particularly lutein is absorbed through enterocytes by simple diffusion or receptor mediated transport. The Current invention also focusses on the making the product stable and amenable to lymphatic absorption by the use of natural oils and particle sizing to make sub-micron particles for uniform dispersion by the application of high pressure super critical carbon dioxide. The conventional method of particle sizing for making liquid dispersion to improve the bioavailability of bioactive have the limitation of such as formulation instability, broad particle size distribution, and low drug loading efficiency. Therefore, the particle designing extended to obtain uniform size distribution by subsequent milling and sieving, To overcome these limitation of current conventional methods, the use of SCF- CO2 as an alternative for fabricating the pharmaceutical and nutraceutical products is most widely used, different variants of the SCF technology for particle fabrication based on the behavior of SCF, such as “solvent” (rapid expansion of supercritical solutions), (Luc Van Ginnekon 2002, Vemavarapu et al. 2005, Mattson D W et al 1987, Fraile M et al 2013) “solute” (particle formation from gas-saturated solutions),“anti-solvent” (supercritical anti-solvent [SAS], The supercritical fluid technology acts as a re- precipitation aid for rapid, uniform, as well as smooth nucleation of solutes. As discussed, the application of supercritical fluid in recent years due to the benefits of micro and a nanoparticle production method has gained a lot of interest. For this technology, Carbon dioxide is the most widely used SCF due to the safety and low cost. Chattopadhyay P, 2007 described the super critically extraction and preparation of lipid nano-suspensions are obtained through the SCF extraction of the organic solvent from O/W emulsions. Solvent extraction into supercritical CO2 leads to the precipitation of lipid/drug material, which is dissolved as composite particles. One of the advantages of this technique is the high solvent extraction efficiency of supercritical CO2, compared to conventional methods, which allows the solvent to be quickly and completely removed and a more uniform particle size distribution to be achieved. As discussed, the method of nano-suspension preparation the rapid expansion of supercritical solvent consists of two stages, i) dissolving the solid substance in a SCF and ii) formation of particles due to supersaturation. For designing particles SCF-CO2 is pumped at desired pressure and temperature to extraction chamber containing solid substance(s) through heat exchanger. The SCF percolates and dissolves the solid substance(s) in the extractor and then the resulted solution is depressurized through a heated nozzle or capillary at supersonic speed into a low-pressure chamber. The expansion of the supercritical solution leads to rapid drop in temperature and pressure and formation of droplets/ particles. The method of rapid expansion of supercritical solution explains the process of particle formation of smaller-sized droplets (SEDS) at the tip of the nozzle. The nozzle and its specifications play a critical role during SEDS processing, which can be altered to adjust the jet breakup for fine particle formation (Zohra et al 2022, Garcia casas 2020, Deepika Purohit 2020, A M Vorobei 2021, Michael turk 2022 and Sandip Roy et al 2021). The various designs of atomizer, such as coaxial nozzles, internal twin-fluid mixing nozzles, four-pinhole nozzle, and an annular gap nozzle. The process is different from the conventional supercritical dispersion as it utilizes a specialized prefilming twin-fluid atomizer that increases the mass transfer rate between the SCF and the solution feed. Further, the mixing of both the liquids, that is, the SCF and the solution feed, is intensified for enhancement of mass transfer rates, which eventually result in fine particles (Mishima K et al 2000, Thakur R et.al 2005.2006; Chioou AHJ et al 2009, Turk M et 2009, Weidner et al 1994, He W Suo et al 2004, 2007 and Chattopadhyay P 2001). The earlier works have also indicated that the existence of atomizing air in the nozzle chamber might result in a faster reduction in the breakup length, and this became even stronger when swirling was imparted to the atomizing air (Pratisinis S E 1988, Kalani A 2002, Debenedetti PG 1990, Friedlander S K et al 2000, and Gupta RB 2006). To improvise the molecular dispersion through SCF-CO2, the solvent enhanced dispersion is most advance process for anti -solvent for particle precipitation. As discussed, the SAS process involves mixing of dispersed multiphase systems and nucleation of solute, SEDS two nozzle and three nozzle design as discussed above for processing biomaterials and pharmaceutical compounds attaining the supersaturation state. The surrounding molecules of the solute in the medium then start accumulating over the nucleus, and the crystal growth ultimately ends with agglomeration. The process is utilized to prepare uniform sized fine particles in single phase equilibrium. For this, the Bioactive nutraceutical/ and or excipient are dissolved in suitable organic solvent by vigorous mixing and thereafter rapidly sprayed over SCF through a coaxial nozzle into a high-pressure vessel leading to uniform small sized droplets due to jet breakup at the tip of the nozzle. However, these are very capital-intensive process and require high capital investment and currently scale up of the same is yet to be implemented. However, currently there are no products available in the market for true solutions at high concentration such as 20%. For designing the particles to make dispersion the SCF based techniques are superior to existing and well established techniques such as milling/crushing for size reduction, and other conventional techniques however the process needs to be implemented at commercial scale. In addition, the cost of the equipment to high which makes process capital intensive. To overcome all these issue in the present invention the SCF-CO2 process utilized for extraction of phytochemicals from plants has been studied for preparing molecular dispersion of the bioactive. The process is defined as Supercritical enabled molecular dispersion/solution SCEMOD. The process utilizes application of vegetable oils particularly palm, sunflower and olive oil. To achieve the supercritical enabled molecular dispersion the plant nutraceuticals are extracted by SCF-CO2 with single extractor and three separators. The extraction conducted at a pressure of from 125-900 bar and most of the trials attempted with 250-900 Bar and 40-80 degree Celsius temperature. For conducting the extraction, the biomass is placed in extractor and CO2 is passed at a rate of 0.5 to 1.5 Kgs/minute. The extraction is conducted by passing 70-100 Kgs SCF-Co2 per Kgs of biomass. The extracted mass is separated and collected. This extract is mixed with vegetable oil ranging from palm oil, canola oil, Sunflower oil and olive oil in a ratio of 0.5:1.5 of the extract. The dispersion of bioactive in the palm, sunflower and olive oils lead to the molecular dispersion. The addition of plant derived phospholipids particularly sunflower lecithin, tocopherol and lower molecular weight polyethylene glycol in a ratio of 0.5-5% were used to further stabilize the molecular dispersion. The molecular dispersion prepared in this way may also contain 2-80% stabilizers. After adding the stabilizers, the oil dispersion is further homogenized in SCF-CO2 by passing SCF-CO2 at rate 0.6-0.8 kg/min at a pressure range of 70-130 bar and 45-60 degree Celsius. The mixing and homogenization in SCF-CO2 vessel carried for 1-1.5h. The homogenized mass is collected and observed under microscope. The microscopic pictures show particle uniform dispersion after mixing with oils and stabilizers. The SCF-CO2 process for extraction and isolation of nutraceutical bioactive overcome the process of solvent residue in the particle designed as devoid of hazardous solvent residue which pose major risk to the health of consumers. In addition, the processing thermo-labile compounds may result in degradation or quality deterioration for preparation of lipid matrix of lipophilic bioactive involves the application of high quantity of surfactants and oils which are absorbed and pass to liver. The bioavailability is very low as the bioactive are metabolized and shows very less active for the health benefits. To address the issue of solvent residue and handling delicate thermo-labile compounds the supercritical fluid carbon dioxide is used as extraction and solvent for particle designing. The supercritical carbon dioxide is environmentally safe and sustainable solvent and overcome the issue of hazardous solvent in the extracted bioactive. The current method proposes the use of carbon dioxide for particle designing by preparing the bioactive in molecular dispersion. The aim of preparing the stable nutraceutical oil suspension is to make stable oil suspension of oil insoluble bioactive for pharmaceutical or nutraceutical applications. The pre-requisite for the stable oil suspension is to bring the oil insoluble ingredients in homogenous suspended form. For long period of time and even if some active settles that could be reconstituted to original homogenous form by slightly mixing. Another approach to prepare oil suspension is by intimate mixing or blending of marker bioactive to be suspended in oil by reducing the suitable particle sizing before suspending in vegetable oils. Therefore, the preparation of oil dispersion enabled with the SCF-CO2 could adhere to the intestinal mucosa thus the contact time of the bioactive dispersed will have enhanced absorption. In spite of the advantages of the process it has certain limitations. The stability of oil dispersions needs to be taken utmost care and require vast formulation skills to ensure the physical stability. The accuracy of doses needs to be ascertained and reliability needs until the suspension is packed in unit doses form. The oil dispersions may be bulky for transport and needs to be shaken each time before measuring a particular dose. As the conventional method of making oil suspension utilize surfactant and co-surfactant in high quality sometimes 100-300% of the bioactive or sometimes same quantity of the bioactive nutraceuticals. Therefore, the oil dispersion prepared in such a way for food application may suffer from the fact of potential toxicity of the surfactant used in high quantity due to the perturbation of intestinal epithelial cells even with the ingredients having GRAS status. Further, the oil dispersions prepared by conventional method the risk assessment is necessary due to the intact bioactive delivery system into the systemic circulation is unlikely due to the intestinal and gastric digestion. The use of RESS and SAS nozzle technologies are yet to be commercially used and require high investment. Moreover, the amount of bioactive dissolved in oil matrix is typically as low as 1%. Therefore, all these issues could be addressed with the used of supercritical enabled molecular dispersion. Further the existing process of making lipids dispersion of bioactives led to transport of bioactives via the portal vein. These bioactives therefore immediately accumulate in the liver and are then metabolized by enzymes, which lowers the concentration of bioactives in the bloodstream. A number of studies conducted showed that the nutraceuticals taken orally typically show low bioavailability due to their degradation by enzymes in the gastrointestinal (GI) tract, and difficulty of absorbing them in the small intestine and the first-pass metabolism in the liver. After oral administration, the bioactives pass through the small intestine and enter the portal vein to liver. Therefore, the metabolism of the nutraceutical bioactives led to very low bioavailability. Another limitation of conventional preparation of lipid dispersion is entrapment of nutrients and bioactive in the lipoproteins resulting in increased the concentration in the plasma. The entrapment of nutrients and bioactive make the slow transport and less bioavailability of the nutrients for absorption. This will lead to poor bioavailability of the nutrients retained in the lipoproteins. A Study conducted by Nishimukai and Hara 2004 showed lymphatic absorption of carotenoids in triglycerides increases the absorption of carotenoids. It has been further studied that the absorption of marigold, lutein ester is improved with high content of fat in the diet. The consumption of low and or high fat meal enriched with vitamin E, alpha carotene an di-lutein ester increased the concentration of these carotenoids in the plasma. Though the other carotenoids require lesser amount of fats in the diet while lutein ester require higher concentration for the similar concentration in the plasma. As discussed above, the lymphatic absorption of carotenoids could deliver the bioactive carotenoids to the target tissues in more active form. This could be accomplished by preparing the carotenoids molecular dispersion in vegetables oils. The dietary nutrients including carotenoids are absorbed in the small intestine and intestinal lymphatic system is used to absorb lipids and dietary lipophilic vitamins as the form of chylomicrons. The molecular dispersion of carotenoids avoids the first pass metabolism. Therefore, owing to the property of olive oil as a carrier for bioactives for lymphatic transport and bypass the first pass metabolism the current invention focusses on the use of vegetable oils particularly olive oil and SCF-CO2 to enable the molecular dispersion for better efficacy and bioavailability. SCEMOD technology converts crystals of lipophilic nutrients into molecular dispersion in an oil carrier. It is different from nanocrystals and nanoparticles, wherein an antisolvent/lipid matrix - or a polymer is required to convert the solution of lipophilic nutrient into nanoparticles or nanocrystals. SCEMOD cannot be compared with RESS (Rapid Expansion Supercritical Solution). Since SCEMOD does not require use of a nozzle or expansion chamber or precipitation chamber. SCEMOD is differentiated from SNEDD (Self-Nano Emulsifying Drug Delivery) because the SNEDD essentially contains a surfactant and/or cosolvent such as methanol/ethanol. SCEMOD can be achieved with just an oil carrier and supercritical co2. SCEMOD is more of a solubilization technique in lipid phase with the help of SCF CO2. SNEDD basically requires that the nutrient should be dissolved in oil phase or cosolvent or surfactant. Products such as Lutein/Curcumin have very low solubility in oils, surfactants and co-solvents. After applying SCEMOD, one can achieve required solubility in oil phase and then proceed with SNEDD to achieve nano-emulsion upon ingestion. SCF CO2 + Olive Lut oil The process of molecular dispersion in lipid matrix for bioactives prepared with food grade vegetable oil and SCF-CO2 is developed as platform technology for different carotenoids, xanthophyll’s, Polyphenols and Diterpene and Omega-3 fatty acids. The process was developed specifically or marigold lutein ester, Turmeric extract powder, Ginger rhizome extract, Astaxanthin and for all fat-soluble nutrients like tocopherol, tocotrienols, Omega-3 fatty acids, carotenoids like lycopene, Vitamin A, D and K and other similar Bioactives. In addition to nutrients, this technology can also be applied to lipophilic drugs, fine chemicals and speciality chemicals. The application of platform technology may help to enhance the bioavailability of poorly soluble lipophilic drug molecules. In the current invention the marigold extract, turmeric extract powder, Astaxanthin and lutein were modified with the help of vegetable oils and Supercritical fluid-CO2 in a modified SCF- CO2 unit at different temperature and pressure. US 6,620,351 B2: The current invention relates to a method for the production of micron or nanometer size particles by precipitation, wherein a dispersion containing the substance of interest is contacted with a Supercritical fluid anti-solvent under near or Supercritical conditions in order to maximize micro or nanoparticle formation. The invention also provides techniques to control the particle size, particle Size distribution and particle morphology. The invention also includes Super critical fluid coating or composite material particle formation, wherein encapsulation of one Substance by another Substance or co-precipitation of more than one Substance in the form of micro or nanoparticles are achieved in the Supercritical fluid anti-solvent. The process defined above may result in the submicron particle though may not lead to molecular dispersion/ true solution of bioactives as compared to current invention. AU-B 70071194: Method and Apparatus for formation of particles - The invention provides a method for the formation of a product which comprises co-introduction of supercritical fluid and a vehicle containing at least one substance in solution or suspension into a particle formation vessel, the temperature and pressure are set and controlled such that dispersion and extraction of the vehicle occur simultaneously by the action of supercritical fluids. The invention also describes product formation and such method for the same and a vessel for carrying out the process. The process of the cited invention results in particle reduction and not solubilization. Though it may lead to lower concentration in solution form, it not comparable to the commercially viable high concentration of bioactive, i.e., 15 to 20%, achieved through the instant invention. US Patent number: 5921478 - The patent describes dispersion method for a solid-liquid system wherein a solid composed of fine particles and a liquid are mixed and dispersed, a dispersion method for a liquid-liquid system wherein two liquids are mixed and emulsified, and a dispersion method for a solid-liquid(water)-liquid (organic solvent) system. The dispersion method is characterized by carrying out the dispersion by using a supercritical solvent in a supercritical state as a dispersing means. The present invention also relates to a dispersing apparatus for the dispersion method. The disadvantage of the cited invention lies in the use of organic solvent while the current invention deals with the green SCF-CO2 for this purpose. CA 2933086C 2016, describes a pharmaceutical composition comprising a chylomicron and a carotenoid. suspended in an aqueous solution and suitable for intravenous administration. The bioavailability of the carotenoid of the pharmaceutical composition is higher relative to the bioavailability of free carotenoid. The process cited mention aqueous dispersion while the current invention rely on the dispersion/ solution of bioactive in lipophilic lipid matrix thereby ensuring improved stability and bioavailability. EP0728037A1 - Method of forming particles using a supercritical fluid, aerogel particles formed thereby, and antiperspirants containing aerogel particles, Gillete Co Inc, 1994: The current invention focuses on the lipophilic lipid matrix for dispersion/ dissolution of bioactive molecules. A method for providing aerogels, and aerogels produced according to the method, is described. As one aspect, antiperspirant compounds that are in aerogel form, and antiperspirant and deodorant compositions including such salts, are described. The method involves contacting a solution containing material to be processed with a species selected to precipitate the material and selected so as to be miscible with the solvent system of the solution. After the material is precipitated, the material may be washed with the precipitating species until it is substantially free of solvent system. Then, the precipitating species containing the material precipitate is taken above its critical point, and the supercritical fluid is exhausted above its critical temperature. This reference does not disclose a method to make a molecular dispersion or true solution of lipophilic bioactives. US Patent 9622497 - The process describes a method of preparation of carotenoid oil suspension by and preparation: a) mixing carotenoid with organic solvent, heating the mixture to dissolve the carotenoid sufficiently to obtain carotenoid solution; b) introducing the carotenoid solution obtained in step a) into a vegetable oil solution stirred in high speed by spraying, meanwhile recovering the organic solvent generated during spraying under vacuum condition, then, simultaneously completing recycling and spraying, thereafter, obtaining carotenoid oil suspension; wherein, the carotenoid oil suspension comprises a carotenoid crystal with an average particle size of less than 5 μm. The method is applicable in industrial scale with continuous operation and increased efficiency without additional carotenoid crystal grinding processes and decreases the degradation of carotenoid during the preparation process of carotenoid oil suspension. The patent describes use of organic solvent for dissolution of carotenoids and results in a suspension of 5-micron crystals. Not comparable to the current invention which focusses on forming a molecular dispersion in oils. EP 2468111A 1- The process describes a composition of carotenoids improved absorption and bioavailability. The process involves preparation of Micellation of xanthophylls after melting Lutein diacetate or Lutein dipropionate in their natural original lipid vegetable matrix, in the presence of lipids, phospholipids, fatty acids, emulsifiers and moisture, and are ingested in a dosage between 2 to 50 mg, to provide an absorption of the carotenoid solubilizate which is at least 20% above than that of crystalline carotenoids, and provides a macular pigment deposit which exceeds at least 10% of Macular Pigment Optical Density, than the deposition obtained by ingesting crystalline Lutein. The formulation developed might prevent tissue degeneration, UV light damage to skin and the retina, and in general to act as more effective and efficient antioxidants than when administered in crystalline form. The cited document describes the comparison of crystalline carotenoid and one in presence of phospholipids, emulsifier, and fatty acids where up to 50 mg of bioactives solubilize, in comparison current investigation aims to prepare lipid matrix of bioactive in vegetable oils and SCF- CO2 to make molecularly dispersed matrix/ true solution at much higher concentration such as 20%, which will have higher absorption and thereby the bioavailability. EP0814112 A2: Surfactants for heterogeneous processes in liquid or supercritical CO2 Keith P. Johnston,1996 Steven P. Wilkinson, Mark Leonard O’Neill, Lloyd Mahlon Robeson, Simon Mawson, Richard Henry Bott & Carrington Duane Smith Heterogeneous polymer mixtures comprising a polymer in liquid or supercritical CO2 are stabilized by employing a poly (propylene oxide) or poly(butylene oxide) based surfactant. These surfactants are especially well suited for stabilizing heterogeneous polymer mixtures formed by micronizing techniques as well as by precipitation of a compressed fluid antisolvent applications. The patent describes use of surfactant in high quantity, which is unacceptable in nutrition industry, while current invention describes the molecular dispersion of bioactive in lipid matrix which high payloads. EP1547679 B1: The invention relates to a method of applying high-intensity ultrasound in order to accelerate and improve the efficiency of the mass transfer method in supercritical fluids. The inventive method comprises the contact separation of a substance containing components that can be extracted with a solvent under supercritical conditions or conditions close to the critical point. The invention also relates to a device which has been developed in order to carry out said method and which is based on the generation of an intense stationary ultrasonic field inside a standard pressurized container for supercritical fluids. The invention further relates to the use of the inventive method for the separation or extraction of natural products which can be used in, for example, the agri-food, chemical, cosmetic and pharmaceutical sectors. The process of cited patent focusses on the mass transfer for better isolation while current method describes molecular dispersion of bioactives in lipophilic oils and SCF-CO2.

Marc Hendricks (2017) Carotenoid bioaccessability in fruit and vegetable-based systems - The process describes release of bioactive carotenoids from the food matrix its solubilisation into a liquid phase and preparation of micelles and uptake by intestine epithelia. However, the limitation of transferring carotenoids in oil phase is limited by food matrix. The presence of oil can facilitate the transfer of oil-to-oil phase thereby increasing the bioaccessability of the bioactive. 6013665 (2000) Stephen J DE Michele et al. The method describes method for enhancing the absorption and transport of lipid Soluble compounds Such as certain Vitamins, nutrients and drugs in an animal. The inventive method comprises the administration of one or more lipid Soluble compounds in conjunction with a structured glyceride component containing at least 33 wt.% of a fatty acid moiety having 4 to 12 carbon atoms, at least 30 wt. % of a fatty acid moiety having more than 12 carbon atoms and an equivalent carbon number (ECN) greater than 30 to less than 48. A study conducted by Yuki Sato et al 2018 showed lutein formulation in self emulsifying suspension prepared with phospholipids resulted in improved intestinal absorption of bioactive lutein after intestinal absorption results in the reformation of micelles. It is reported that transport of lutein through lymphatic route resulted in low plasma concentration. It has been further studied by Annet JC Rodenburg et al 2000, that plasma concentration was higher when lutein is consumed with high fat spread (207% increase) as compared to low fat spread 80% increase indicating that improved lutein absorption in presence of fatty oils. This process of manufacturing water soluble formulation is requiring specified equipment and its scale up implementation will be problematic. The work reported by Hyeji Ahn and Ji-Ho Park 2016, that the soluble drugs are preferentially transported via the portal vein and immediately accumulate in the liver and are immediately metabolized by enzymes thereby lower the concentration in the blood plasma. However, an alternative route for delivering drugs to the systemic circulation is the intestinal lymphatic pathway. Lipophilic drugs are known to be transported via the lymphatic system. The intestinal lymphatic pathway can bypass first- pass metabolism in the liver, thus increasing drug bioavailability. Furthermore, the co- administration of drugs with lipids can enhance their lymphatic transport. In a postprandial state, lipid–drug conjugates and lipid-based nanoparticles have been widely studied for the delivery of lipophilic drugs via the lymphatic pathway. This will increase the active concentration in the blood plasma which will have more beneficial health impact. In the current investigation the nutraceutical bioactives are homogenized with olive oil under supercritical condition which converts the bioactives to molecular dispersion and transported to the targeted delivery. The other studies conducted revealed that oleic acid micelles and olive oil (oleic acid, C18:1) enhance the intestinal accessibility of carotenoids more than linoleic acid micelles or vegetables oils rich in polyunsaturated fatty acids (PUFA). The current work also focusses on the use of lipophilic oils particularly olive oil for targeting lymphatic absorption to improve the bioavailability. Rangaswamy Lakshminarayana and Valiikanan Baskaran 2013 found the Carotenoids incorporated into the lipid phase, are emulsified into small lipid droplets in the stomach and transferred further to mixed micelles formed by the action of bile salts, biliary phospholipids, dietary lipids, and their hydrolysis products. H Tapiero 2004 found that the mixed micelles containing carotenoids migrate through the brush border by simple diffusion, where carotenoids are absorbed by the intestinal enterocytes, packed into chylomicrons, and secreted to the lymphatic system for circulating in the blood stream studied that the carotenoids on digestion are incorporated into the lipid phase and then are emulsified into small lipid droplets. The nature and amount of lipids in the diet greatly affect the emulsification, secretion of bile salts, and formation of mixed. The study found that the oleic acid micelles and olive oil (oleic acid, C18:1) enhance the intestinal accessibility of carotenoids more than linoleic acid micelles or vegetables oils rich in polyunsaturated fatty acids (PUFA). As reported the consumption of tomato products with olive will but not with sunflower oil improve the antioxidant activity in the plasma indicating olive oil as more efficient carrier as compared to sunflower oil. The role of carried lipid in the carotenoid absorption was also studied by Kopec et al 2014, for absorption and conversion of Provitamin A in Avocado oil. Further the particle size reduction through micellarization was found to increase the intestinal digestion of beta carotene in presence for various lipids in the order of soybean oil > olive > canola > butter, However for the absorption of xanthophylls lutein and Zeaxanthin the carrier oil impact was not reported as studies by Failla Mark Chitchumronchokchai, Chureeporn,Ferruzzi Mario G, Goltz Shellen R 2014.

Consumption of carotenoids, tomato products with olive oil but not with sunflower oil improves the antioxidant activity of the plasma [Lee A et al 2000 & H Tapiero, D N Townsend 2004]. A similar study conducted on two-day old calves at a dose of 25mg of lutein dispersed in either olive oil or corn oil, the peak plasma concentration was achieved in 10-12h. The analysis of carotene content in the lymph found the absorption of alpha tocopherol from corn oil was 50 % lesser as compared with coconut oil. Indicating the impact of lipid in the absorption of particular carotenoid. It has also been reported that the olive oil is essential for carotenoids absorption in the intestine and its incorporation in the micelles. A study conducted by Annie Harison Dunn, 2022 on the absorption of lutein in Olive oil and coconut oil, when 200 micro-mole of lutein extracted from marigold meal is fed in oil to the mice compared with control group of micelle lutein fed the olive, coconut, ground nut, palm, and ground nut oil the Plasma level of lutein was highest in olive oil 82 % followed by coconut oil 68%.Lutein accumulation in liver and eye was also highest in olive oil120% liver and 116% eye.in coconut oil 105% and 109% in liver and eye respectively. The intestinal lutein level were higher than the control group by 167% for olive oil, 159% for coconut oil, followed by sunflower oil 140% and groundnut oil 123%, soybean oil 108%, Rice bran oil 87% and palm oil 36%. US Patent 4048203 describes a method for producing a 1% solution of lutein-fatty acid ester by dissolving in hot mono- and diglycerides. The resulting solution is homogenized with an equal volume of 0.5% solution of Polysorbate 80 (TWEEN" 80, ICI United States Inc., Wilmington, Delaware 19897). The emulsion thus produced can be used for coloring ice cream by direct addition to mixing. The above reference has a limitation of low concentration, such as 1% in solution form. Moreover, it makes use of an organic solvent to solubilize lutein ester, which is unacceptable in nutrition industry. Summary of the Invention: The invention describes a molecular dispersion or true solution of lipophilic bioactives in oil matrix in high concentration such as 20% which is made possible through application of supercritical fluid carbon dioxide. The invention further provides for method by which the molecular dispersion is stabilized, such that upon ingestion, the molecular dispersion instantly forms a Nano-emulsion in stomach without allowing crystallization of lipophilic bioactive. According to an aspect of the present invention, there is provided a method for preparing molecular dispersion of a lipophilic bioactive in a lipid matrix, said method comprising the step of: extracting the lipophilic bioactive material with food grade oils (lipid matrix) into a supercritical carbon dioxide (SCF-CO2) extraction vessel; mixing and homogenizing the plant bioactive material with the food grade oils by passing the supercritical carbon dioxide (SCF-CO2) in the extraction vessel, at a specified pressure and temperature for a time duration of 2-3h; stabilizing the molecular dispersion of lipophilic bioactive material with the help of stabilizers and additives; wherein, the supercritical carbon dioxide (SCF-CO2) extraction vessel incorporates tubing and rings that facilitates the uniform distribution of carbon dioxide during extraction and molecular dispersion process, and results in sizing of the particle to molecular level which makes the dispersion homogenous. In accordance with one embodiment of the present invention, the lipophilic bioactive material is extracted from the plant material. In accordance with one embodiment of the present invention, the plant material having particle sizes ranging from 1-4 mm. In accordance with one embodiment of the present invention, the above mentioned method further includes a step of conducting microscopic observation to verify the quality of the molecular dispersion. In accordance with one embodiment of the present invention, the lipophilic bioactive material and food grade oils (lipid matrix) in SCF-CO2 extraction vessel in a proportion 1:0.5 to 1:1. In accordance with one embodiment of the present invention, the lipophilic bioactive material selected from the group consisting of lutein ester, turmeric extract powder; curcumin 95%, and astaxanthin, and wherein the lipophilic bioactive material are present in a ratio of 1:0.5 to 1:1 with respect to the food grade oils. In accordance with one embodiment of the present invention, the molecular dispersion of the bioactive material is performed at a pressure having range from 60-900 bar. In accordance with one embodiment of the present invention, the molecular dispersion of the bioactive material is performed at a temperature having range from 40-80 degree Celsius. In accordance with one embodiment of the present invention, lipophilic bioactive material selected from the group consisting of carotenoids, curcumin, ginger, omega 3 fatty acids, tocopherols, tocotrienols, Coenzyme Q10, Vitamin C palmitate and vitamin A, K and the like. In accordance with one embodiment of the present invention, said food grade oils (lipid matrix) is selected from the group consisting of sunflower oil, safflower oil, soybean oil, corn oil, canola oil, castor oil, ground nut oil, palm and the like. In accordance with one embodiment of the present invention, the antioxidants and stabilizers are present in an amount from 0.2 to 0.5%, and 2-20%, respectively. The antioxidants and stabilizers are selected from tocopherol, ascorbyl palmitate, rosemary extract, and the like. In accordance with one embodiment of the present invention, said additives includes PEG 400, and plant-derived phospholipids. In accordance with one embodiment of the present invention, the stabilizers added in step (c) range from 2% to 80% by weight to improved water dispersibility of the bioactive. In accordance with one embodiment of the present invention, the supercritical carbon dioxide (SCF-CO2) extraction vessel comprises high-pressure CO2 pumps which delivers CO2, at flow rates ranging from 0.5 to 2.0 Kg/min. In accordance with one embodiment of the present invention, the method for the preparation of composition is aimed at the lymphatic transport of SCEMOD preparation and specially target retina with the application of higher chain fatty acids like palm oil, sunflower oil and olive oil. In accordance with one embodiment of the present invention, the retinal target is accompanied by improved bioavailability with the mixing of palm oil, sunflower and/or olive oil to the botanicals for preparing molecular dispersion. According to one aspect of the present invention, there is provided a composition comprising: a lipid matrix formed of a food or pharma grade oil in an amount from 40 to 50 percent by weight of composition; a lipophilic bioactive dispersed at a molecular level in the lipid matrix, wherein the lipophilic bioactives are present in a concentration of at least 20%; and a stabilizer and additives for enhancing the homogenous dispersion and stability of the lipophilic bioactive in the lipid matrix, said stabilizer are present in an amount from 2 to 20 percent; wherein the composition is prepared by using supercritical carbon dioxide as a solvent, the composition is prepared by dispersing the lipophilic bioactive in the lipid matrix at a molecular level in an extraction vessel that operates within a pressure range of 125-900 bar and a temperature range of 40-80 degrees Celsius. Detailed Description of the Invention: The current invention mentions the preparation of molecular dispersion of bioactive nutraceuticals with oil and SCF-CO2 in a specially designed SCF-CO2 equipment. For the preparation of molecular dispersion, the plant material is run in SCF-CO2 with oils for solubilizing the bioactives in presence of SCF-CO2. The extractor is modified by adding small mixing SS tubes/packings which help in uniform distribution of Carbon dioxide during extraction/ molecular dispersion. The specific experimental trials for preparing molecular dispersion are described below. The marigold meal processed from marigold flower is input for conducting SCF-CO2 extraction at different pressure and temperature. For enabling the molecular dispersion of lipophilic extract single stage and /or two stage extraction is carried out. The initial stage involves removal of oil and waxes followed by extraction to get enriched content lutein ester in the SCF_CO2. In two stage extraction the enriched lutein ester-marigold extract is homogenized with vegetable oil ranging from palm oil, MCT oil, Soybean oil, Sunflower oil and olive oil to get lutein ester dispersed in the oil. Further to improvise the dispersion of marigold extract done with the SCF-Co2 is further added stabilizer and additives and further run in SCF-CO2 at specified temperature and pressure. Botanicals for preparation of SCEMOD were grinded to make particle size ranging from 1-4mm and the same was loaded to the SCF-CO2 extraction vessel, 12L capacity. The jacket heating is conducted with hot water circulation. The extraction parameter were set 250-900 bar pressure and 40-80 degree Celsius temperature. For enabling extractionSCF-CO2 is passed at a rate of 0.5 kgs-2.0/minute. The total Co2 passed is generally depends upon the nature of herbal material and the particle size. The extraction material is collected in the separator-1 while the lower content extract is collected in separator-2 while oil and waxes are collected in separator-3. The temperature and pressure of three separators is set depending on the nature of purification required. For marigold extraction the separator -1, separator-2 and separator-3 are maintained at a pressure ranging from 90-350 bar and 50-70 degree celsius, 50-140 bar and 45-50 degree Celsius and 40-70 bar and 10-15 degree celsius respectively. The High-pressure extractors as used in the present investigation are designed independently as parallel systems connected each to independent high pressure CO ₂ pumps having CO ₂ flow rate up to 0.5-2.0 Kg/min with a design pressure of both pumps and extractors 900 bar while the respective H.P, M.P and L.P separators are designed to pressures of 450-600, 240-350 and 83-110 bar. The extraction of botanical powder form of 1-4 mm particle size is conducted by placing the botanical in a basket which is further loaded into an extractor vessel. In the process of extraction, the extractors are confined to various extraction pressures between pressures of operation 150-600 bar specifically 250-590 bar while temperatures are controlled using heat exchangers in-between 45- 65 ⁰ C. Though, the extractions are also attempted at a temperature higher than 65 ⁰ C, for the purpose of invention, higher temperatures 80 ⁰ C are not preferable as the facts that the carotenoids are highly susceptible for temperatures and oxygenated-air. In the present investigation, the pressure parameters of each extractor and separators are regulated to maintain the set pressure conditions by high pressure auto control valves in concordance of temperatures regulation through heat exchangers. Separator-1 collect lutein ester with high content 25-35 % while separator-2 contain Lutein ester content from 8-15% and separator-3 contain 1-2% lutein ester. The vegetable oils are added to the marigold meal however the collected extract contain lower content of lutein ester generally in the range of 15-20%. To overcome the content of lutein ester two phase extraction was carried out first conducting the extraction at 250-590 bar and collecting the lutein ester in separator-1, While the lutein ester with lesser content is collected in separator-2 while oil and water is collected in separator-3. The extract thus obtained is mixed with vegetable oil (1:0.5 to 1:1) ranging from sunflower oil, Palm oil, Soybean oil and olive oil. Stabilizers and antioxidants are added to the oil suspension to get the homogenous molecular dispersion. For conducting the extraction, the SCF-CO2 is passed at a Flow rate of 0.5-0.8 Kg/minute and the homogenization temperature and pressure are set a 90-160 bar and temperature 45-65 degree celsius. SCF-CO2 is passed approximately 40-50 kgs/h of the bioactives taken for molecular dispersion through homogenization. After passing the required amount of SCf_Co2 the homogenized oil dispersion is collected and observed microscopically. Astaxanthin extracted from Haematococcus pluvialis at a pressure of 590-900 bar and 45-60 degree Celsius is filled to the extraction vessel. Vegetable oil sunflower, palm oil, and olive oil are used alone individually. The oil is added to the SCF-CO2 extract in a proportion of 0.5-1.5 volume and homogenization conducted. After passing and maintaining at a pressure of 100-110 bar and temperature 45-60 degree celsius the homogenized molecular mass is collected. The microscopic observation is conducted. All the molecular dispersion of nutraceutical may or may not added stabilizer to improvise the water dispersibility of the bioactives. In the current work the stabilizers used 2-80%to ensure the better dispersibility. The antioxidants ranging from tocopherol, ascorbyl palmitate, rosemary extract along with pharmaceutical additive PEG 400 and plant derived phospholipids, sunflower oil used also used. The application of all ingredients ensures the proper dispersion along with stability of the SCEMOD prepared. Modified design of Extraction vessel- The extraction vessel/ equipment was modified by incorporation small SS tubing which were placed at the bottom of the vessel used for preparing molecular dispersion. The SCF-CO2 passed through these tubing’s at high pressure and temperature result in sizing of the particle to molecular level and making the dispersion homogenous. FIG 2 discloses a SCF-CO2 Extraction Equipment for preparing molecular dispersion, in accordance with one embodiment of the present invention. The SCF-CO2 extraction vessel was modified for better distribution of extraction solvent SCF-CO2. The S-S tubing’s and rings were used at the bottom and towards the periphery of the vessel to pass the pressurized CO2 under supercritical condition for particle modification and produced properly dispersed mass in liquid matrix. The SCF-CO2 extraction of bioactives The analysis of lutein ester content in the dispersed mass is carried out by UV-Vis spectrophotometer as well as by High pressure liquid chromatography method. Example 1 2 kgs marigold meal is loaded to SCF-CO2 basket and extraction conducted at 550-770 bar pressure and 55–70 degree Celsius temperature. The SCF-CO2 is passed at a rate of 0.6-1.0 Kgs/min for one hour. The marigold oil containing waxes and water is separated in three separators. The content of active in the bioactive extract was 17-29.9% in the different experiment conducted. The results of all trials taken are presented in Table 1. Example 2: 2 kgs marigold meal is loaded to SCF-CO2 basket and two stage SCF-CO2 extraction was conducted at 125 -250 bar and 550 Bar temperature, 65-73 degree celsius and 65- 70 degree celsius respectively. The SCF-CO2 is passed at a rate of 0.8-1.2 Kgs/min for 2-3 hour. The marigold extract is collected in separator-1 and oil and water is collected in separator-2 and separtator-3 respectively. The extract is collected and analysed by Spectrophotometry and shows 6.78% Lutein ester in first stages while the lutein ester in second stage is 32.03%. The first stage extract shows molecular dispersion in water while second stage shows lesser dispersibility. Example 3: 2 kgs marigold meal is loaded to SCF-CO2 basket and two stage SCF-CO2 extraction was conducted at 325 bar and 750 Bar temperature, 65 degree celsius and 65-70 degree celsius respectively. The SCF-CO2 is passed at a rate of 0.8-1.2 Kgs/min for 1.5 hour. The marigold extract is collected in separator-1 and oil and water is collected in separator-2 and separtator-3 respectively. The extract is collected and analysed by Spectrophotometry and shows 9.0% Lutein ester in both the stages with good dispersibility. Example 4: 2 kgs marigold meal is loaded to SCF-CO2 basket with the addition of 200g ethanol and 200g olive oil to it and SCF-CO2 extraction was conducted at 550-800 bar pressure and 65-70 celsius temperature. The SCF-CO2 is passed at a rate of 1.0-1.1 Kgs/min for 2.5 hour. The marigold extract is collected in separator-1 and oil and water is collected in separator-2 and separtator-3 respectively. The extract is collected and analysed by Spectrophotometry and shows 12.16% Lutein ester with slight dispersibility. Example 5: Extract obtained from two stage extraction having bioactive content 40% is further charged in SCF-CO2 liquid-liquid extractor and further added vegetable oils sunflower, MCT, Soybean, palm and olive oil, the CO@ is run on dynamic mode at a pressure 120 bar and 50 degree celsius for 1-1.5h. The extract shows good dispersibility. The extraction data is presented in Table 3. The extract is further added 210-35% stabilizers for enabling complete dispersion. The extract so obtained shows excellent dispersibility in water as presented in microscopic figures. Example 6: The marigold extracts as obtained in Example2 is further charged in SCF-CO2 liquid- liquid extractor and added olive oil 0.8 to 1.0 volume of marigold extract and homogenized by passing SCF-CO2 at a flow rate of 1 kg/minute for one hour. This results in a homogenous molecular dispersion of marigold meal extract. The extract is further added stabilizers and antioxidants to further improvise the dispersion. Tocopheryl acetate at 0.2-0.5% of the extract, Sunflower lecithin 2-3%, Polysorbate 80, 1-2% and Koliphor RH 40 at 12-30% of the extract Volume of the extract charged. SCF-CO2 is passed at a rate of 0.5-1.0Kg/min for 1-1.5 hour at a pressure ranging 80-100 Bar and temperature 40-50 degree celsius to get homogenous molecular dispersion. The extract is unloaded from the extractor and content of lutein ester measured by spectrophotometry. The lutein ester content is 15.6%. Example 7: 300g curcumin extract 95% is added 1100 g MCT oil and placed in SCF-CO2 liquid extract for enabling the molecular dispersion. The pressure and temperature are controlled at 180 bar and 70 degree celsius. SCF_CO2 is passed at 1kg/minute and total 80 kgs SCF-CO2 is passed. The extract is taken out and curcumin content in the lipid matrix is 21.5%. Example 8: 500g Curcumin extract 95% is added 1800g olive oil and charged to SCF-CO2 extractor. The extraction/ molecular dispersion trials were conducted at 180 bar pressure and 80degree celsius temperature. For enabling the dispersion 120 kgs SCF-CO2 is passed at a flow rate of 1.0 Kgs/minute. The extract shows 20.1% curcumin content as presented in table 5. Example 9: For enabling molecular dispersion of Astaxanthin oleoresin 200g of same is added 200g sunflower oil and charged to SCF-CO2 high pressure vessel adding the mixing balls to the same. The vessel pressure and temperature are maintained at 160 Bar and 60 degree celsius. The 390g extract and analysed for Astaxanthin content showing 10.04% content. Example 10: In the SCF-CO2 vessel 200g Astaxanthin oleoresin 20.1% and 200g olive oil are charged. To stabilize 2g mixed tocopherol is also added. The pressure and temperature are maintained at 160 bar and 50 degree celsius. The SCF-CO2 passed 500 Kgs and the content of Astaxanthin is 11.25%. The lipid matrix shows good dispersibility indicating the role of olive oil and SCF-CO2 enabling the molecular dispersion. Example 11 The 1.0 refined SCF-CO2 extract of marigold is filled in the Liquid-liquid extractor and added 1.00 Kgs sunflower oil and homogenized at a pressure of 80-100 degree celsius. Further stabilizer and additives for enabling improved molecular dispersion are added, Plant phospholipid, Sunflower phospholipids 25g, Polyethylene glycol 25g, Polysorbate 80, 25-50g and Koliphor RH 100g. The SCF-CO2 is passed at a rate of 1kg/minute for one hour. After depressurization the molecular dispersed lutein ester is collected, and analysis done spectrophotometrically showing 18% lutein ester. A. Primary extraction of Lutein ester oleoresin with Marigold Meal in SCFE Image: A: It shows that crystals of lipophilic nutrients uniform dispersion in fatty oleoresin matrix. Image B1: Initial molecular dispersion of Image B2: Final liquid dispersion/ solution carotenoids of Carotenoids in olive oil 1:1 of marigold d The Microscopic pictures of marigold lutein ester after adding 1:1 volume of different oil and SCF-CO2 particle formation for enabling sub-micron particles and finally with stabilizers is illustrated in fig 1(a) to 1(j).