Title:
SUPERNATANT OF BROWN ADIPOCYTES, METHOD FOR PREPARING SAME AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/036184
Kind Code:
A1
Abstract:
Provided is a composition that comprises a supernatant of brown adipocytes or a purified product thereof and has a metabolism improving effect. Also provided is a method for preparing the aforesaid supernatant without using a liquid culture containing glucose at a high concentration. Also provided is a method for producing brown adipocytes using pluripotent stem cells, said method being useful for preparing a supernatant of brown adipocytes. In the present invention, a supernatant having a metabolism improving effect was successfully obtained from brown adipocytes. Also, this supernatant could be obtained without using a liquid culture containing glucose at a high concentration. In the present invention, moreover, brown adipocytes were successfully formed from pluripotent stem cells using a feeder-free culture system without adding a cytokine cocktail, etc. The supernatant of brown adipocytes according to the present invention is expected as applicable to the development of therapeutic drugs for sugar metabolism-related diseases and cosmetics.
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Inventors:
SAEKI KUMIKO (JP)
KOBAYASHI NORIHIKO (JP)
OKA MASAKO (JP)
MATSUMURA KAZUNORI (JP)
NISHIO MIWAKO (JP)
SAEKI KOICHI (JP)
SHU TSUGUMINE (JP)
MORI TOYOTAKA (JP)
KOBAYASHI NORIHIKO (JP)
OKA MASAKO (JP)
MATSUMURA KAZUNORI (JP)
NISHIO MIWAKO (JP)
SAEKI KOICHI (JP)
SHU TSUGUMINE (JP)
MORI TOYOTAKA (JP)
Application Number:
PCT/JP2019/031897
Publication Date:
February 20, 2020
Filing Date:
August 14, 2019
Export Citation:
Assignee:
NAT CENTER FOR GLOBAL HEALTH AND MEDICINE (JP)
ID PHARMA CO LTD (JP)
ID PHARMA CO LTD (JP)
International Classes:
A61K35/35; A61K35/545; A61P3/00; A61P3/04; A61P3/10; A61P5/50; A61P43/00; C12N5/074
Domestic Patent References:
| WO2012147853A1 | 2012-11-01 | |||
| WO2012147853A1 | 2012-11-01 | |||
| WO2007069666A1 | 2007-06-21 | |||
| WO2010008054A1 | 2010-01-21 |
Foreign References:
| JP2018108080A | 2018-07-12 |
Other References:
NISOLI, E. ET AL.: "Expression of Nerve Growth Factor in Brown Adipose Tissue: Implications for Thermogenesis and Obesity", ENDOCRINOLOGY, vol. 137, no. 2, February 1996 (1996-02-01), pages 495 - 503, XP055686688
NISHIO, M. ET AL.: "Production of Functional Classical Brown Adipocytes from Human Pluripotent Stem Cells using Specific Hemopoietin Cocktail without Gene Transfer", CELL METABOLISM, vol. 16, 2012, pages 394 - 406, XP055142155, DOI: 10.1016/j.cmet.2012.08.001
SAEKI,KUMIKO: "Production of brown adipocytes from human pluripotent stem cells", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 250, no. 9, 1 September 2014 (2014-09-01), pages 816 - 821, XP009526444, ISSN: 0039-2359
SAEKI,KUMIKO: "Function of brown adipocytes established from human ES/iPS cells", THE LIPID, vol. 25, no. 1, 2014, pages 74 - 80, XP009526443, ISSN: 0915-6607
GLOBAL BURDEN OF DISEASES, INJURIES, AND RISK FACTORS STUDY, 2017
"The GBD 2015 Obesity Collaborators", NEW ENGLAND JOURNAL OF MEDICINE, vol. 377, 2017, pages 13 - 27
YU ET AL., ANNALS OF INTERNAL MEDICINE, vol. 166, 2017, pages 613 - 620
FARRAR ET AL., BMJ, vol. 355, 2016, pages i5953
VILLAMOR ET AL., JAMA, vol. 317, 2017, pages 2515 - 2523
PERSSON ET AL., BMJ, vol. 357, 2017, pages J2563
NAVAR ET AL., JAMA, vol. 316, 2016, pages 2041 - 2043
ALEGRE-DIAZ ET AL., NEW ENGLAND JOURNAL OF MEDICINE, vol. 375, 2016, pages 1961 - 1971
SCHAUER ET AL., NEW ENGLAND JOURNAL OF MEDICINE, vol. 376, 2017, pages 641 - 651
VAN MARKEN LICHTENBELT ET AL., NEW ENGLAND JOURNAL OF MEDICINE, vol. 360, 2009, pages 15091 - 1525
SAITO ET AL., DIABETES, vol. 58, 2009, pages 1526 - 1531
KOBAYASHI ET AL., MEDICAL RESEARCH ARCHIVE, vol. 4, 2016
NISHIO ET AL., CELL METABOLISM, vol. 16, 2012, pages 394 - 406
YONESHIRO ET AL., OBESITY, vol. 19, 2011, pages 1755 - 1760
BEIJER ET AL., ONCOL REV, vol. 6, 2012
CARSON ET AL., EXERC SPORT SCI REV, vol. 38, 2010, pages 168 - 176
GALAT ET AL., STEM CELL RESEARCH & THERAPY, vol. 8, no. 67, 2017
ICHISAKA T ET AL., NATURE, vol. 448, no. 7151, 2007, pages 1015 - 1021
EMANI ET AL., THE JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, vol. 154, 2017, pages 286 - 289
THOMOU ET AL., NATURE, vol. 542, 2017, pages 450 - 455
SUEMORI ET AL., BIOCHEM BIOPHYS RES COMMUN, vol. 345, 2006, pages 926 - 932
TAKAHASHI K ET AL., CELL, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 72
NAKAGAWA M ET AL., SCIENTIFIC REPORTS, vol. 4, 2014, pages 3594
MIYAZAKI ET AL., ENDOCRINOLOGY, vol. 127, 1990, pages 126 - 132
See also references of EP 3838279A4
NISHIO, M. ET AL.: "Production of Functional Classical Brown Adipocytes from Human Pluripotent Stem Cells using Specific Hemopoietin Cocktail without Gene Transfer", CELL METABOLISM, vol. 16, 2012, pages 394 - 406, XP055142155, DOI: 10.1016/j.cmet.2012.08.001
SAEKI,KUMIKO: "Production of brown adipocytes from human pluripotent stem cells", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 250, no. 9, 1 September 2014 (2014-09-01), pages 816 - 821, XP009526444, ISSN: 0039-2359
SAEKI,KUMIKO: "Function of brown adipocytes established from human ES/iPS cells", THE LIPID, vol. 25, no. 1, 2014, pages 74 - 80, XP009526443, ISSN: 0915-6607
GLOBAL BURDEN OF DISEASES, INJURIES, AND RISK FACTORS STUDY, 2017
"The GBD 2015 Obesity Collaborators", NEW ENGLAND JOURNAL OF MEDICINE, vol. 377, 2017, pages 13 - 27
YU ET AL., ANNALS OF INTERNAL MEDICINE, vol. 166, 2017, pages 613 - 620
FARRAR ET AL., BMJ, vol. 355, 2016, pages i5953
VILLAMOR ET AL., JAMA, vol. 317, 2017, pages 2515 - 2523
PERSSON ET AL., BMJ, vol. 357, 2017, pages J2563
NAVAR ET AL., JAMA, vol. 316, 2016, pages 2041 - 2043
ALEGRE-DIAZ ET AL., NEW ENGLAND JOURNAL OF MEDICINE, vol. 375, 2016, pages 1961 - 1971
SCHAUER ET AL., NEW ENGLAND JOURNAL OF MEDICINE, vol. 376, 2017, pages 641 - 651
VAN MARKEN LICHTENBELT ET AL., NEW ENGLAND JOURNAL OF MEDICINE, vol. 360, 2009, pages 15091 - 1525
SAITO ET AL., DIABETES, vol. 58, 2009, pages 1526 - 1531
KOBAYASHI ET AL., MEDICAL RESEARCH ARCHIVE, vol. 4, 2016
NISHIO ET AL., CELL METABOLISM, vol. 16, 2012, pages 394 - 406
YONESHIRO ET AL., OBESITY, vol. 19, 2011, pages 1755 - 1760
BEIJER ET AL., ONCOL REV, vol. 6, 2012
CARSON ET AL., EXERC SPORT SCI REV, vol. 38, 2010, pages 168 - 176
GALAT ET AL., STEM CELL RESEARCH & THERAPY, vol. 8, no. 67, 2017
ICHISAKA T ET AL., NATURE, vol. 448, no. 7151, 2007, pages 1015 - 1021
EMANI ET AL., THE JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, vol. 154, 2017, pages 286 - 289
THOMOU ET AL., NATURE, vol. 542, 2017, pages 450 - 455
SUEMORI ET AL., BIOCHEM BIOPHYS RES COMMUN, vol. 345, 2006, pages 926 - 932
TAKAHASHI K ET AL., CELL, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 72
NAKAGAWA M ET AL., SCIENTIFIC REPORTS, vol. 4, 2014, pages 3594
MIYAZAKI ET AL., ENDOCRINOLOGY, vol. 127, 1990, pages 126 - 132
See also references of EP 3838279A4
Attorney, Agent or Firm:
HARUNA, Masao et al. (JP)
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