Title:
SUSPENSION FOR PARALLEL REACTION, PARALLEL REACTION METHOD, AND SCREENING METHOD
Document Type and Number:
WIPO Patent Application WO/2013/024821
Kind Code:
A1
Abstract:
[Problem] To provide a suspension for a parallel reaction, a parallel reaction using the suspension, and a screening method that utilizes the parallel reaction. [Solution] Gel particles made of low-melting agarose have a retiform structure due to the hydrogen bonds between polysaccharide chains; single-stranded DNA having a length within a range of 15 to 50 bases, which functions as a nucleic acid amplification reaction primer, and nitrilotriacetic acid (NTA), as a substance that recognizes a protein having a specific tag sequence, are immobilized in a portion of the retiform structure.
Inventors:
ICHIKAWA SOSAKU (JP)
YAMAMOTO TATSUYUKI (JP)
SAEKI DAISUKE (JP)
SUGIURA SHINJI (JP)
KANAMORI TOSHIYUKI (JP)
YAMAMOTO TATSUYUKI (JP)
SAEKI DAISUKE (JP)
SUGIURA SHINJI (JP)
KANAMORI TOSHIYUKI (JP)
Application Number:
PCT/JP2012/070531
Publication Date:
February 21, 2013
Filing Date:
August 10, 2012
Export Citation:
Assignee:
UNIV TSUKUBA (JP)
NAT INST OF ADVANCED IND SCIEN (JP)
ICHIKAWA SOSAKU (JP)
YAMAMOTO TATSUYUKI (JP)
SAEKI DAISUKE (JP)
SUGIURA SHINJI (JP)
KANAMORI TOSHIYUKI (JP)
NAT INST OF ADVANCED IND SCIEN (JP)
ICHIKAWA SOSAKU (JP)
YAMAMOTO TATSUYUKI (JP)
SAEKI DAISUKE (JP)
SUGIURA SHINJI (JP)
KANAMORI TOSHIYUKI (JP)
International Classes:
C12N15/09; C12Q1/68; G01N33/53
Domestic Patent References:
| WO2010087121A1 | 2010-08-05 |
Foreign References:
| JP2009540829A | 2009-11-26 | |||
| JP2004536565A | 2004-12-09 | |||
| JP2012170346A | 2012-09-10 |
Other References:
KUMARESAN, PALANI ET AL.: "High-throughput single copy DNA amplification and cell analysis in engineered nanoliter droplets.", ANALYTICAL CHEMISTRY, vol. 80, no. 10, 2008, pages 3522 - 3529
RUNGPRAGAYPHAN, SUANG ET AL.: "High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.", JOURNAL OF MOLECULAR BIOLOGY, vol. 318, no. 2, 2002, pages 395 - 405
DAISUKE SAEKI ET AL.: "Hantomei Micro Capsule o Mochiita Musaibo Tanpakushitsu Goseikei no Micro Compartment-ka", ABSTRACTS OF ANNUAL MEETING OF THE SOCIETY OF CHEMICAL ENGINEERS, vol. 76, 22 February 2011 (2011-02-22), JAPAN, pages 325
RUNGPRAGAYPHAN, SUANG ET AL.: "High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.", JOURNAL OF MOLECULAR BIOLOGY, vol. 318, no. 2, 2002, pages 395 - 405
DAISUKE SAEKI ET AL.: "Hantomei Micro Capsule o Mochiita Musaibo Tanpakushitsu Goseikei no Micro Compartment-ka", ABSTRACTS OF ANNUAL MEETING OF THE SOCIETY OF CHEMICAL ENGINEERS, vol. 76, 22 February 2011 (2011-02-22), JAPAN, pages 325
Attorney, Agent or Firm:
KOYAMA, YUU (JP)
Hill 有 (JP)
Hill 有 (JP)
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Claims: