0001. This Application is a continuation in part of U.S. Patent Application No. 12/466,801 filed on May 15, 2009, which has one inventor in common. U.S. Patent Application No. 12/466,801 is incorporated herein in its entirety by reference.

BACKGROUND OF THE INVENTION

1. AREA OF THE INVENTION

0002. The present invention is related to how to treat mites material derived from Swinglea glutinosa leaves and a

preparation made of the material derived from Swinglea glutinosa leaves and avermectins. The extract is described by preceding pending unpiblished U.S. Patent Application No. 12/466,801.

2. DESCRIPTION OF PRIOR ART

0003. Mite pests affect a range of agronomic, vegetable and fruit cultivations causing great losses. Avermectin mixes, such as abamectin have been use to control mites. Because,

resistance by mites to avermectins is common, increasingly high doses are being used to control these acari. In addition, in order to combat resistance, avermectins are used in combination with synthetic pesticides, e.g., pyrethroids .

0004. Unfortunately, there is no description in the prior art of a natural derived compound that can be used in a mix with avermectins to diminish resistance by mites to avermectins, or to avoid using synthetic pyrethroids . The Invention of the present Application overcomes these prior art limitations.

SUMMARY OF THE INVENTION

0005. The invention of the present application provides a natural material derived from Swinglea glutinosa leaves that in combination with avermectins, increases the effect of

avermectins over mites. The material derived from Swinglea glutinosa leaves, in a preparation with avermectins, results in an all natural material with the potential to replace

combinations of avermectins with synthetic pyrethroids,

eliminating the need to use synthetic pesticides for acari control .

0006. More specifically, the invention of the present

application provides a preparation that comprises a material derived from Swinglea glutinosa leaves and avermectins.

0007. In an aspect of the present invention, the preparation is used to treat acari .

0008. In one additional aspect of the present invention, the acari is Tetranichus sp.

0009. In another aspect of the present invention, the

avermectins of the preparation are constituted by abamectin. 0010. In one more aspect of the preparation of the present invention, the material derived from Swinglea glutinosa leaves is an extract obtained by a method comprising:

A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;

B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;

C. breaking up the leaves into small fragments;

D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;

E. repeat step D. until the solvent is enriched with

substances derived from the leaf fragments; and,

F. retiring the solvent to release an extract.

0011. Objectives and advantages of the present Application invention will be more evident in the detailed description of the invention and the claims.

DETAILED DESCRIPTION OF THE INVENTION

0012. the invention of the present application provides preparation that comprises a material derived from Swingl glutinosa leaves and avermectins.

0013. In an aspect of the present invention, the preparation is used to treat acari . 0014. In one additional aspect of the present invention, the acari is Tetranichus sp.

0015. In another aspect of the present invention, the

avermectins of the preparation are constituted by abamectin.

0016. In one more aspect of the preparation of the present invention, the material derived from Swinglea glutinosa leaves is an extract obtained by a method comprising:

A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;

B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle ;

C. breaking up the leaves into small fragments;

D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;

E. repeat step D. until the solvent is enriched with

substances derived from the leaf fragments; and,

F. retiring the solvent to release an extract.

0017. The term acari includes:

Polyphagotarsonemus sp.

Aculops sp.

Brevipalpus sp .

Aceria sp. Phyllocoptruta sp.

Epitrimerus sp.

Tetranychus sp.

Panonychus sp .

Phyllocnistis sp .

Liriomyza sp .

Scrobipalpula sp.

Psylla sp.

Boophilus sp. ,

and other species describe as acari .

0018. In one more aspect of the Swinglea glutinosa extract of the present invention, in the method, the leaves must not be broken into leaf fragments that are less than 0.5 mm, since smaller fragments would tend to become a single mass which will become a limitation for the optimal extraction with a solvent.

0019. In all cases where a solvent is mentioned in this application, the solvent that can be used comprises ethanol, methanol, hexane, propanol, isopropanol, C0 ₂ , acetone, water, ethyl -acetate, nitrile-acetate, toluene, tetrahydrofurane , Chloroform, dichloromethane , and others.

0020. Objectives and advantages of the present Application invention will be more evident in the detailed description of the invention and the claims.

EXAMPLES

0021. A test was made by putting 5mm discs cut from leaves of bean plants in Petri plates, wherein a the 5mm leaf discs were bathed with: 1) Water only; 2) 2ml of Swinglea glutinosa

Extract/Liter; 3) a solution of a preparation made of 2ml of Swinglea glutinosa Extract/Liter + abamectin 0.1 to 0.3 ml/Liter; and 4) Abamectin 0.1 to 0.3 ml/Liter. 10 Tetranichus sp. individual mites were put on top of each of the 5mm leaf discs. Water surrounded the 5mm leaf discs in the Petri plates to avoid the mites from escaping the discs. Mortality of

Tetranichus sp. mites was measured after 24 hours and 48 hours. All the experiments were repeated three times and each

experiment consisted in a set of three Petri plates for each variable for a total of 24 plates per experiment.

0022. The results are shown in the following Table TABLE 1. Mortality of Tetranichus sp. Mites

TREATMENT Average percentage Average percentage

Mortality 24 hours Mortality 48 hours

Water 5.9 % 10.1 %

SS 9.3 % 12.4 %

SS + 0.1 Aba 15.0 % 17.2 %

Aba 0.1 6.7 % 6.7 %

SS + 0.2 Aba 8.3 % 18.3 %

0.2 Aba 8.3 % 10.0 %

SS + 0.3 Aba 18.3 % 26.7 %

0.3 Aba 0.0 % 5.7 % SS = Swingle glutinosa extract 2ml/L

Aba = Abamectin ml/L

0023. Table 1. shows that in all cases, the preparation with Swinglea glutinosa + abamectin combination resulted in a higher mite mortality than the mite mortality when abamectin was used alone .

0024. In conclusion, these results suggest that the use of Swinglea glutinosa in combination with abamectin to control mites is better than abamectin alone at 0.1 to 0.3 doses.