Field of the Invention This invention relates to a new and advantageous process for the synthesis of 1 -(2-((2,4- dimethylphenyl)thio)phenyl)piperazine (vortioxetine) a drug for the treatment of depression and anxiety.

Background of the Invention Vortioxetine is disclosed as Example 1 e in WO 2003/029232 A1 and is described as being prepared analogously to Example 1 . The process used to prepare Example 1 involves the preparation of 1 -(2-((2-(trifluoromethyl)phenyl)thio)phenyl)piperazine on a solid polystyrene support, followed by decomplexation using visible light irradiation, and purification by preparative LC-MS and ion-exchange chromatography. The overall yield for the preparation of vortioxetine is described as 17%.

Several alternative palladium catalyzed processes for the preparation of vortioxetine are described in Examples 17 to 25 of WO 2007/144005 A1 . These processes describe the preparation of vortioxetine from 2,4-dimethylthiophenol and 2-bromoiodobenzene (or 1 ,2- dibromobenzene) starting materials via a 1 -(2-bromo-phenylsulfanyl)-2,4-dimethyl-benzene intermediate. Each of these processes involves the use of a palladium catalyst and a phosphine ligand.

The preparation of vortioxetine is also described by Bang-Andersen et al. in J. Med. Chem. (201 1 ), Vol. 54, 3206-3221 . Here, in a first step, te/t-butyl 4-(2-bromophenyl)piperazine-1 - carboxylate intermediate is prepared from Boc-piperazine and 2-bromoiodobenzene in a palladium catalyzed coupling reaction. te/t-Butyl 4-(2-bromophenyl)piperazine-1 -carboxylate is then reacted with 2,4-dimethylthiophenol, again in the presence of palladium catalyst and a phosphine ligand, to provide Boc-protected vortioxetine. In the final step, vortioxetine is deprotected using hydrochloric acid to give vortioxetine hydrochloride.

WO 2013/102573 A1 describes a reaction between 1 -halogen-2,4-dimethyl-phenyl, 2-halogen- thiophenol and an optionally protected piperazine in the presence of a base and a palladium catalyst consisting of a palladium source and a phosphine ligand.

Each of the above processes has disadvantages. The process described in WO 2003/029232 is low yielding and unsuitable for the large scale production of vortioxetine, whereas the processes described in WO 2007/144005 A1 , WO 2013/102573 A1 and by Bang-Andersen et al. require the use of expensive starting materials, palladium catalyst and phosphine ligand. In addition, the toxicity of palladium is well known, Liu et al. Toxicity of Palladium, Toxicology Letters, 4 (1979) 469-473, and the European Medicines Agency' s Guideline on the Specification for Residues of Metal Catalysts sets clear limits on the permitted daily exposure to palladium arising from palladium residue within drug substances, www.ema.europa.eu. Thus it would be desirable to avoid the use of a palladium catalyst in the synthesis of vortioxetine and the subsequent purification steps required to remove palladium residue from the final pharmaceutical product.

Summary of the Invention

Various aspects, advantageous features and preferred embodiments of the present invention as summarized in the following items, respectively alone or in combination, contribute to solving the object of the invention. (1 ) A process for the preparation of a compound of formula VII, or a salt thereof,

said process comprising the steps of

a) reacting a compound of formula V

V

with piperazine in a presence of a copper catalyst, and

b) optionally, converting the obtained compound of formula VII into the salt thereof.

The process according to item 1 , wherein a Cu(l) salt is used as the copper catalyst.

The process according to item 2, wherein the Cu(l) salt is selected from the group consisting of Cul, CuBr, CuCI and Cu ₂ 0. The process according to item 2 or item 3, wherein the copper catalyst additionally comprises a ligand.

The process according to item 4, wherein the ligand is selected from the group consisting of amino acids, bipyridines, diamines, phenols, alcohols, 1 H-benzotriazole, 2-((2,6- dimethylphenyl)amino)-2-oxoacetic acid, and 2-isobutyrylcyclohexanone.

The process according to item 5, wherein the ligand is 2-phenylphenol or N,N-diethyl-2- hydroxybenzamide.

The process according to item 1 , wherein in step a) a base is present.

The term "base" as employed herein means a proton acceptor, preferably a water soluble proton acceptor or an alcoholate salt such as an alkali metal terf-butanolate salt, more preferably the proton acceptor is selected from the group consisting of carbonate salts, and hydroxides of alkaline or earth alkaline metals. The preferred bases are K ₃ P0 ₄ , Cs ₂ C0 ₃ and K ₂ C0 ₃ .

The process according to item 7, wherein the base is K ₃ P0 ₄ .

The process according to item 1 , wherein step a) is conducted at a temperature in a range from 20 ^< €to 200 ^< €.

The process according to item 9, wherein the temperature is about 120°C.

The term "about" means approximately, in the region of, roughly, or around. When the term is used in conjunction with a numerical range, it modifies that range by extending the boundaries above and below the numerical values set forth. In general, the term "about" is used herein to modify a numerical value above and below the stated value by a variance of 10 %. ) The process according to item 1 , wherein the compound of formula V is prepared by reacting a compound of formul wherein X represents F or NH

with a compound of formula II

II

(12) The process according to item 1 , wherein the compound of formula V is prepared by reacting a compound of formul

Γ

with a compound of formula Ι

II'

(13) The process according to item 12, wherein said process is conducted in a presence of a copper catalyst.

(14) The process according to item 1 , wherein the compound of formula V is prepared by reacting a compound of formul

with nitrite, and

reacting the obtained diazo compound of formula IV

IV with a compound of formula Ml, wherein M is selected from the group consisting of K, Na, Li, Cs, Zn, Cu, NH ₄ , and Bu ₄ N, or with a compound of formula Nl ₂ , wherein N is selected from the group consisting of Mg, Ca, Ba, Fe, Cu, Ni, and Sn, or with All ₃ .

(15) The process according to item 14, wherein the obtained diazo compound of formula IV is reacted with a compound of formula Ml, wherein M is selected from the group consisting of K, Na, Li, Cs, Zn, Cu, NH ₄ , and Bu ₄ N, preferably M is K.

(16) The process according to item 14, wherein the obtained diazo compound of formula IV is reacted with a compound of formula Nl ₂ , wherein N is selected from the group consisting of Mg, Ca, Ba, Fe, Cu, Ni, and Sn.

(17) The process according to item 14, wherein the compound of formula III is prepared by reacting a compound of formula I" with a compound of formula II" wherein Y represents Br or I.

(18) The process according to item 14, wherein the compound of formula III is prepared by a) reacting a compound of form "

r

wherein Z represents F or CI,

with a compound of formula

II to obtain the compound of formula III'

III' , and

b) reducing the compound of formula III' to obtain the compound of formula III.

The process according to any one of the previous items, wherein the salt of the compound of formula VII is a hydrobromide salt or a hydrochloride salt.

A compound of formula V

V

(21 ) Use of the compound as defined in item 20 in the preparation of vortioxetine or a salt thereof.

(22) A process for the preparation of a pharmaceutical composition comprising a compound of formula VII, or a salt thereof

comprising the steps of:

x) preparing a compound of formula VII, or a salt thereof, by carrying out a process according to any one of items 1 to 19, and y) mixing the compound of formula VII, or a salt thereof, with a pharmaceutically acceptable carrier and/or excipient.

The new process defined above provides vortioxetine, or salts thereof, in a high yield without the need of using the homogeneous catalysis with palladium catalysts and phosphine ligands, nor expensive starting materials, which provides an industrially applicable, environmentally friendly and economically improved process for obtaining vortioxetine, or salts thereof. The present invention further provides a new intermediate compound that is highly useful as a key intermediate in the preparation of vortioxetine or salts thereof. The intermediate is solid, crystalline, suitable for optional purification by precipitation or preferably recrystallization.

Detailed Description of the Invention

The invention is described below in further detail by embodiments, without being limited thereto. A general concept of the process of the present invention may be represented in Scheme 1 .

Scheme 1 : General representation of the basic synthetic concept of the present invention.

The compound of formula V, i.e. (2,4-dimethylphenyl)(2-iodophenyl)sulfane, is reacted with piperazine (compound of formula VI) in the presence of a copper catalyst to form the compound of formula VII (vortioxetine). The reaction may be conducted in the presence of an organic or inorganic base, for example, K ₂ C0 ₃ , Cs ₂ C0 ₃ , Na ₂ C0 ₃ , K ₃ P0 ₄ , NaOtBu, KOtBu, NaOH, KOH, Et ₃ N, Et ₂ NiPr, Bu ₃ N, triethanolamine, DBU, DABCO, or the like, in a polar or non-polar solvent. Suitable solvents are DMSO, DMF, NMP, dioxane, toluene, iPrOH. The temperature of the reaction should be in a range of from 20 ^ to 200 °C, preferably the reaction temperature is about 120 °C.

The copper catalyst used is, for example, Cu(l) salt, such as Cul, CuBr, CuCI or Cu ₂ 0. The reaction presented in Scheme 1 can be performed without a ligand. Its presence, however, increases the reaction speed. Most commonly used ligands are amino acids, for example, proline, glycine, sarcosine (N-methylglycine), Ν,Ν-dimethylglycine; bypyridines, for example, 2,2'-bipyridyl, 1 ,10-phenanthroline, neocuproine (2,9-dimethyl[1 ,10]phenanthroline); diamines, for example, cis- or trans-1 ,2-diaminocyclohexane, cis- or trans-N,N'-dimethyl-1 ,2- cyclohexanediamine, 2-methylpropane-1 ,2-diamine, Ν,Ν'-dimethylethylenediamine, Ν,Ν,Ν',Ν'- tetramethylethylene-diamine; phenols, for example, 2,6-dimethylphenol, 2-phenylphenol, 1 ,1 '- bi(2-naphthol), 8-quinolinol, N,N-diethyl-2-hydroxybenzamide; alcohols, for example, ethane- 1 ,2-diol, 1 ,1 ,1 -tris-(hydroxylmethyl)ethane; and other types of ligands such as 1 H-benzotriazole, 2-((2,6-dimethyl-phenyl)amino)-2-oxoacetic acid, and 2-isobutyrylcyclohexanone. Preferably used ligands are 2-phenylphenol and N,N-diethyl-2-hydroxybenzamide. Optionally, the vortioxetine compound obtained by the process of the present invention can also be converted to a salt thereof, for example to a hydrobromide salt (VM.HBr) or a hydrochloride salt (VII. HCI).

In a particular embodiment, the compound of formula V may be prepared by a reaction illustrated in Scheme 2.

X = NH ₂ : lb

Scheme 2: Representation of a particular synthetic embodiment of the present invention.

Intermediate V can be prepared according to simple nucleophilic aromatic substitution of fluorine atom in compound la with 2,4-dimethylbenzenethiol (II). It can also be prepared according to Sandmeyer reaction conditions starting from 2-iodoaniline (lb) and 2,4- dimethylbenzenethiol (Ma) or starting from 2-iodobenzenethiol ( ) and 2,4-dimethylaniline (Ι ).

Alternatively, (2,4-Dimethylphenyl)(2-iodophenyl)sulfane (V) can also be prepared according to the reaction path presented in Scheme 3 through 2-((2,4-dimethylphenyl)thio)aniline (III), which can be prepared directly from 2-aminobenzenethiol (I") coupled with 1 -bromo-2,4- dimethylbenzene (M"a) or 1 -iodo-2,4-dimethylbenzene (M"b) using different types of heavy metal catalysts, for example, Pd, Cu, Rh, Co, Fe, Ni, In, La, Zr. Most commonly used heavy metals for such transformations are Pd and Cu.

Compound III can also be prepared from 2,4-dimethylbenzenethiol (II) and 1 -fluoro-2- nitrobenzene (l"'a) or 1 -chloro-2-nitrobenzene (l'"b). In the first step (2,4-dimethylphenyl)(2- nitrophenyl)sulfane (III') is formed and in the second reaction step nitro group is reduced to ami

Z = F: l"'a

Z = CI: l"'b

Scheme 3: Representation of a particular synthetic embodiment of the present invention.

Compound III obtained as described above is then transformed to (2,4-dimethylphenyl)(2- iodophenyl)sulfane (V) using Sandmeyer reaction conditions. This can be achieved by adding an acidic medium, for example, H ₂ S0 ₄ or HCI, to a solution of III, which is followed by addition of an inorganic metal nitrite compound, for example, NaN0 ₂ . The reaction is conducted in water or a mixture of water and polar organic solvent, for example MeCN, at a temperature in a range from 0°C to 10°C. The obtained diazo compound is then reacted with Ml, Nl ₂ or All ₃ , wherein M is selected from the group consisting of K, Na, Li, Cs, Zn, Cu, NH ₄ , and Bu ₄ N, and wherein N is selected from the group consisting of Mg, Ca, Ba, Fe, Cu, Ni, and Sn. Finally, the compound of formula V is obtained at a temperature of about 25 °C. The processes described above allow for preparation of a crystalline intermediate V from cheap starting materials without using toxic palladium catalysts.

In a specific embodiment presented in Scheme 4, the 1 -fluoro-2-iodobenzene (la) reacts with 2,4-dimethylbenzenethiol (II) in the presence of a base, preferably K ₂ C0 ₃ or Cs ₂ C0 ₃ , in a polar solvent, preferably DMSO or DMF, at reaction temperatures in the range from 80 °C to 200 °C, preferably the temperature is about 140°C. This reaction, i.e. the aromatic nucleophilic substitution results in affording the (2,4-dimethylphenyl)(2-iodophenyl)sulfane (V).

la

Scheme 4: Synthesis of intermediate V representing the embodiment of the invention.

In a further specific embodiment shown in Scheme 5, the compound of formula V can be prepared from either 2-iodoaniline (lb) or 2,4-dimethylaniline (Ι ). First, the compound lb or ΙΓ, respectively, is dissolved at about 0°C in an acidic media, preferably in the aqueous solution of HCI or H ₂ S0 ₄ . To the obtained solution NaN0 ₂ is added. Finally, a water solution of potassium or sodium salt of 2,4-dimethylbenzenethiol (II) or 2-iodobenzenethiol ( ) is then slowly added at O'C to yield the compound V.

lb V

Scheme 5: Synthesis of intermediate V representing the embodiment of the invention.

In a further specific embodiment, the compound of formula V is prepared by reacting the 2- aminobenzenethiol (I") and 1 -iodo-2,4-dimethylbenzene (M"a) (Scheme 6). These compounds are coupled in a polar solvent, preferably DMSO, using heavy metal catalyst, preferably copper catalyst, for example Cul, in a presence of a base, preferably K ₂ C0 ₃ , to give intermediate III. The preferred reaction temperature is about 120°C. (2,4-Dimethylphenyl)(2-iodophenyl)sulfane (V) is then prepared according to standard Sandmeyer reaction conditions from compound III, which is first transformed to corresponding diazo compound using diazotizing agent, preferably NaN0 ₂ , in a presence of an acid, preferably HCI or H ₂ S0 ₄ , at 0°C in water or a mixture of water and appropriate polar organic solvent, preferably MeCN. Finally, an aqueous solution of potassium or sodium iodide is slowly added to the solution/mixture at about O 'C. Compound V can be isolated in a crystalline form.

III V

Scheme 6: Synthesis of an intermediate V representing the embodiment of the invention.

Vortioxetine (VII) can be prepared from (2,4-dimethylphenyl)(2-iodophenyl)sulfane (V) by heavy metal catalyzed coupling with piperazine (VI).

As shown in the reference examples 1 and 2, Pd-catalyzed coupling could be used to obtain vortioxetine from intermediate V. In this coupling any palladium(ll) source could be used like Pddba ₂ , Pd ₂ dba ₃ , Pd(OAc) ₂ , PdCI ₂ , PdBr ₂ , etc. This transformation occurs in the presence of phosphine (monophosphine or diphosphine) ligands, and also in the presence of carbene-type ligands. For example, Pddba ₂ /rac-BINAP could be used as a catalytic system.

However, as mentioned above, it is desirable to avoid the use of a palladium catalyst in the synthesis of vortioxetine and the subsequent purification steps required to remove palladium residue from the final pharmaceutical product.

Hence, according to the process of the present invention, the compound of formula V is coupled with piperazine (VI) in a presence of a copper catalyst, which is made possible due to the presence of a more reactive iodine atom in the compound V.

Conversely, and as shown by Comparative Examples 1 and 2, the Cu-catalyzed coupling does not work if (2-bromophenyl)(2,4-dimethylphenyl)sulfane (V) is used.

In a specific embodiment presented in Scheme 7, vortioxetine (VII) can be prepared by reacting the compound of formula V with piperazine (VI) in the presence of Cul and a ligand, preferably 2-phenylphenol or N,N-diethyl-2-hydroxybenzamide. The reaction is performed in a polar solvent, preferably in DMSO, in a presence of a base, preferably K ₃ P0 ₄ , in a temperature range of from 20 ^< C to 200 ^< C, preferably the reaction temperature is about 120°C.

ligand: 2-phenylphenol or N,N-diethyl-2-hydroxybenzamide

Scheme 7: Synthesis of vortioxetine (VII) representing the embodiment of the invention using

Cu-ligand catalytic system. The vortioxetine compound, or a salt thereof, prepared according to the invention may be used or administered on its own, preferably it is administered as a pharmaceutical composition comprising vortioxetine, or a salt thereof, and a pharmaceutically acceptable excipient and/or carrier. Further, the vortioxetine compound, or a salt thereof, prepared according to the invention may be combined with other drugs, especially with drugs used for the treatment of depression and anxiety.

In a further aspect of the present invention, a pharmaceutical composition comprising the compound of formula VII (vortioxetine), or a salt thereof, is prepared by comprising the steps of preparing the compound of formula VII, or a salt thereof, as described above, and mixing the compound of formula VII, or a salt thereof, with a pharmaceutically acceptable carrier and/or excipient. The administration form can be suitably chosen, e.g. a form suitable for oral, parenteral, rectal administration and/or administration by inhalation, and the dosage form may be solid, liquid, or powdery. Therefore, the pharmaceutical composition comprising vortioxetine, or a salt thereof, prepared according to the invention may suitably be in the form of tablets, pills, capsules, syrups, powders or granules for oral administration; or as sterile parenteral or subcutaneous solutions, suspensions for parenteral administration; or as suppositories for rectal administration.

Suitable excipients and/or carriers include, without being limited to, diluents, binders, disintegrants, lubricants, etc. For example, the compound or a finely divided form thereof, or particles comprising the compound, are mixed with a carrier or binder substance, e.g. a mono-, di- or polysaccharide such as sugars and starch, a sugar alcohol or another polyol. For example, lactose, saccharose, sorbitol, mannitol, starch, cellulose derivatives, a binder such as polyvinylpyrrolidone, and a lubricant such as magnesium stearate, calcium stearate, polyethylene glycol, waxes, paraffin, and the like are mixed, and then compressed into tablets. The compound or a finely divided form thereof, or particles containing the same may be coated by another substance. The powder mixture or particles containing the compound may also be dispensed into capsules.

The pharmaceutical composition comprising vortioxetine, or a salt thereof, prepared according to the invention in a desired dose is generally suitable to treat a disease or condition of a patient in need thereof, specifically to display a desired activity when treating the depression and anxiety.

Further embodiment of the present invention resides in the provision of a valuable intermediate compound V useful in the synthesis of a formula VII (vortioxetine)

V

The following Examples are non-limiting and serve to illustrate the invention.

Experimental Procedures

Example 1 : Preparation of 2-((2,4-dimethylphenyl)thio)aniline (III) from 1 -fluoro-2-nitrobenzene

(l'"a) or 1 -chloro-2-nitrobenzene (l"'b) and 2,4-dimethylbenzenethiol (II) followed by nitro reduction.

l'"a II III

To a the mixture of K ₂ C0 ₃ (125 g, 0.90 mol) in dry DMF (0.5 L) while stirring at 25 °C was slowly added 2,4-dimethylbenzenethiol II (1 13 ml_, 0.84 mol), then 1 -fluoro-2-nitrobenzene l'"a (1 16 g, 0.82 mol) was added in 2 h. After stirring the reaction mixture for 30 min 1 L water was added, and obtained mixture was stirred at 25^ for 1 h. Yellow precipitate was then filtered off, washed with water (2 x 0.5 L) and dried in vacuum at 50 °C to afford title compound III' as yellow crystals (209 g, 98% yield): DSC: Onset 93.34 ^< €, Peak 97.39 ^< €; H NMR (CDCI ₃ , 500 MHz) δ 2.30 (s, 3H), 2.40 (s, 3H), 6.71 (dd, J = 1 .2, 8.2 Hz, 1 H), 7.1 1 (m, 1 H), 7.18-7.22 (m, 2H), 7.32 (m, 1 H), 7.46 (d, J = (ESI) m/z: 260 [MH] ⁺ .

To a solution of 1 -chloro-2-nitrobenzene l'"b (5.00 g, 31 .7 mmol) in dry DMF (30 mL) K ₂ C0 ₃ (5.26 g, 38 mmol) and 2,4-dimethylbenzenethiol II (4.50 mL, 33.3 mmol) were added, and resulting reaction mixture was stirred at 25^ for 18 h. Water (60 mL) was added and obtained mixture was stirred at 25^ for 30 min. Yellow precipitate was then filtered off, washed with water (2 x 50 mL) and dried to afford title compound III' as yellow crystals (8.24 g, total yield): H NMR (CDCI ₃ , 500 MHz) 5 2.30 (s, 3H), 2.40 (s, 3H), 6.71 (dd, J = 1 .2, 8.2 Hz, 1 H), 7.1 1 (m, 1 H), 7.18-7.22 (m, 2H), 7.32 (m, 1 H), 7.46 (d, J = 7.8 Hz, 1 H), 8.25 (dd, J = 1 .4, 8.2 Hz, 1 H); MS (ESI) m/z: 260 [MH] ⁺ .

To a mixture of (2,4-dimethylphenyl)(2-nitrophenyl)sulfane III' (10.0 g, 38.6 mmol) and AcOH (100 mL) Fe (8.61 g, 154 mmol) was added, and resulting reaction mixture was stirred at 30 'Ό for 16 h. Reaction mixture was then filtered through a bed of Celite, and filtrate was concentrated. To the residue 300 mL saturated NaHC0 ₃ and 100 mL EtOAc was added. Organic layer was separated, water layer was washed with EtOAc (100 mL), combined organic layers were dried over Na ₂ S0 ₄ , and solvent was evaporated to afford title compound III as orange oil (8.85 g, 99% yield): H NMR (CDCI ₃ , 500 MHz) δ 2.28 (s, 3H), 2.40 (s, 3H), 4.24 (br s, 2H), 6.71 (d, J = 8.1 Hz, 1 H), 6.75-6.81 (m, 2H), 6.87 (m, 1 H), 7.02 (m, 1 H), 7.23 (m, 1 H), 7.38 (dd, J = 1 .5, 7.7 Hz, 1 H); MS (ESI) m/z: 230 [MH] ⁺ . Example 2: Preparation of 2-((2,4-dimethylphenyl)thio)aniline (III) from 2-aminobenzenethiol (I")

I" ll"a

Mixture of 2-aminobenzenethiol I" (1 .07 mL, 10.0 mmol), 1 -iodo-2,4-dimethylbenzene M"a (1 .43 mL, 10.0 mmol), Cul (0.19 g, 1 .0 mmol), K ₂ C0 ₃ (6.90 g, 50.0 mmol), and dry and degasses DMSO (50 mL) was stirred under nitrogen at 120°C for 16 h. Reaction mixture was cooled to room temperature, water (50 mL) was added and the product was extracted to EtOAc (2 x 150 mL). Combined organic layers were washed with water (3 x 150 mL) and brine (3 x 150 mL). Organic phase was then dried over MgS0 ₄ and solvent was evaporated to afford title compound as a dark oil (2.15 g, 94% yield): H NMR (CDCI ₃ , 500 MHz) δ 2.28 (s, 3H), 2.40 (s, 3H), 4.24 (br s, 2H), 6.71 (d, J = 8.1 Hz, 1 H), 6.75-6.81 (m, 2H), 6.87 (m, 1 H), 7.02 (m, 1 H), 7.23 (m, 1 H), 7.38 (dd, J = 1 .5, 7.7 Hz, 1 H); MS (ESI) m/z: 230 [MH] ⁺ .

Example 3: Preparation of (2,4-dimethylphenyl)(2-iodophenyl)sulfane (V) from

dimethylphenyl)thio)aniline (III)

v

To a mixture of 2-((2,4-dimethylphenyl)thio)aniline III (1 .83 g, 8.0 mmol) in water (8 mL) slowly H ₂ S0 ₄ (96%, 1 .2 mL) was added. Obtained mixture was cooled to O 'C and solution of NaN0 ₂ (0.61 g, 8.8 mmol) in water (2 mL) was added slowly. After addition was complete MeCN (5 mL) was added and obtained mixture was stirred at 0°C for 30 min. This solution was then slowly added to an ice-cold aqueous (4 mL water) solution of Kl (1 .74 g, 10.5 mmol). Obtained mixture was stirred at 25^ for 16 h. EtOAc (20 mL) was then added, organic layer was separated, washed with water (3 x 50 mL) and Na ₂ S ₂ 0 ₅ (aq., 25 mL), and dried over MgS0 ₄ containing C (1 g) and silicagel (1 g). Salts were then filtered off, washed with EtOAc, and filtrate was concentrated to afford title compound V as an orange oil which crystalized upon standing (2.47 g, 91 % yield): H NMR (CDCI ₃ , 500 MHz) δ 2.33 (s, 3H), 2.37 (s, 3H), 6.58 (dd, J = 1 .5, 8.0 Hz, 1 H), 6.81 (m, 1 H), 7.06 (m, 1 H), 7.13 (m, 1 H), 7.17 (m, 1 H), 7.39 (d, J = 7.8 Hz, 1 H), 7.80 (dd, J = 1 .3, 7.8 Hz, 1 H); MS (ESI) m/z: 341 [MH] ⁺ . Example 4: Preparation of (2,4-dimethylphenyl)(2-iodophenyl)sulfane (III) from 1 -fluoro-2- iodobenzene (la)

la

Mixture of 1 -fluoro-2-iodobenzene la (0.58 mL, 5.0 mmol), 2,4-dimethylbenzenethiol II (0.68 mL, 5.0 mmol) and Cs ₂ C0 ₃ (1 .71 g, 5.25 mmol) in dry and degasses DMF (2 mL) was stirred under nitrogen at 140°C for 3 days. Reaction mixture was cooled to room temperature, water (25 mL) was then added and the product was extracted to EtOAc (2 x 20 mL). Combined organic layers were washed with brine (2 x 20 mL) and dried over MgS0 ₄ . After evaporation of the solvent title compound V was isolated as brown oil (1 .29 g, 75% yield): H NMR (CDCI ₃ , 500 MHz) δ 2.33 (s, 3H), 2.37 (s, 3H), 6.58 (dd, J = 1 .5, 8.0 Hz, 1 H), 6.81 (m, 1 H), 7.06 (m, 1 H), 7.13 (m, 1 H), 7.17 (m, 1 H), 7.39 (d, J = 7.8 Hz, 1 H), 7.80 (dd, J = 1 .3, 7.8 Hz, 1 H); MS (ESI) mlz: 341 [MH] ⁺ .

Example 5: Preparation of (2,4-dimethylphenyl)(2-iodophenyl)sulfane (III) from 2-iodoaniline

(lb)

To a mixture of ice (2.5 g) and 37% HCI (2.1 mL) 2-iodoaniline lb (2.74 g, 12.5 mmol) is slowly added. After obtained mixture was stirred at O 'C for 15 min, aqueous (2.15 mL water) solution of NaN0 ₂ (0.88 g, 12.75 mmol) was slowly added. While obtained solution was stirred at 0°C for 30 min solution of KOH (2.8 g, 50.0 mmol), 2,4-dimethylbenzenethiol II (2.03 mL, 15.0 mmol) in water (5 mL) was prepared. To this solution previously prepared solution with diazonium salt was slowly added. Obtained reaction mixture was stirred at 5°C for 1 h, then it was heated to 80°C and stirred for 20 h. Reaction mixture was then cooled to room temperature, water (20 mL) was added and the product was extracted to MeTHF (2 x 20 mL). Combined organic layers were washed with 1 M HCI (3 x 20 mL) and brine (2x 20 mL). Organic phase was separated, dried over MgS0 ₄ and solvent was evaporated. Obtained crude product was purified by chromatography (MeCy) to afford title compound as orange oil (2.35 g, 55% yield): H NMR (CDCI ₃ , 500 MHz) 5 2.33 (s, 3H), 2.37 (s, 3H), 6.58 (dd, J = 1 .5, 8.0 Hz, 1 H), 6.81 (m, 1 H), 7.06 (m, 1 H), 7.13 (m, 1 H), 7.17 (m, 1 H), 7.39 (d, J = 7.8 Hz, 1 H), 7.80 (dd, J = 1 .3, 7.8 Hz, 1 H); MS (ESI) m/z: 341 [MH] ⁺ .

Example 6: Preparation of (2,4-dimethylphenyl)(2-iodophenyl)sulfane (V) from 2- iodobenzenethiol ( )

I' II'

To a mixture of H ₂ S0 ₄ (96%, 2.0 mL) and water (10 mL) 2,4-dimethylaniline ΙΓ (1 .54 mL, 12.5 mmol) was slowly added. After cooling to Ο 'Ό, NaN0 ₂ (0.88 g, 12.75 mmol) was slowly added. After stirring at 0°C for 30 min, it was slowly added to an ice-cold solution of 2-iodobenzenethiol (Γ) (3.0 g, 12.75 mmol) and KOH (5.6 g, 100 mmol) in water (15 mL). Obtained reaction mixture was stirred at 5°C for 1 h, then MeCN (20 mL) was added, solution was heated to 70 ^ and stirred for 16 h. Water (50 mL) was then added, the product was extracted to EtOAc (3 x 25 mL), combined organic layers were dried over MgS0 ₄ . After evaporation of the solvent crude product was purified by chromatography (MeCy) to afford title compound as orange oil which crystalized upon standing (2.60 g, 61 % yield): H NMR (CDCI ₃ , 500 MHz) δ 2.33 (s, 3H), 2.37 (s, 3H), 6.58 (dd, J = 1 .5, 8.0 Hz, 1 H), 6.81 (m, 1 H), 7.06 (m, 1 H), 7.13 (m, 1 H), 7.17 (m, 1 H), 7.39 (d, J = 7.8 Hz, 1 H), 7.80 (dd, J = 1 .3, 7.8 Hz, 1 H); MS (ESI) mlz: 341 [MH] ⁺ .

Example 7: Preparation of 1 -(2-((2,4-dimethylphenyl)thio)phenyl)piperazine vortioxetine, VII)

Mixture of (2,4-dimethylphenyl)(2-iodophenyl)sulfane V (0.34 g, 1 .0 mmol), piperazine VI (0.13 g, 1 .5 mmol), K ₃ P0 ₃ (0.42 g, 2.0 mmol), Cul (19 mg, 0.1 mmol), and 2-phenylphenol (68 mg, 0.4 mmol) in dry and degassed DMSO (2 mL) was heated under nitrogen atmosphere at 120°C for 20 h. Water (10 mL) is then added and product is extracted to EtOAc (3 x 10 mL). Combined organic layers were washed with water (3 x 10 mL) and brine (2 x 10 mL) and dried over Na ₂ S0 ₄ . After evaporation of the solvent crude product is purified by chromatography to afford title compound: H NMR (CDCI ₃ , 500 MHz) δ 1 .63 (br s, 1 H), 2.33 (s, 3H), 2.37 (s, 3H), 3.02- 3.09 (m, 8H), 6.52 (m, 1 H), 6.87 (m, 1 H), 7.04 (m, 1 H), 7.06-7.10 (m, 2H), 7.16 (m, 1 H), 7.39 (d, J= 7.8 Hz, 1 H); MS (ESI) m/z: 299 [MH] ⁺ .

Example 8: Preparation of 1 -(2-((2,4-dimethylphenyl)thio)phenyl)piperazine (vortioxetine, VII)

Mixture of (2,4-dimethylphenyl)(2-iodophenyl)sulfane V (0.34 g, 1 .0 mmol), piperazine VI (0.13 g, 1 .5 mmol), K ₃ P0 ₃ (0.42 g, 2.0 mmol), Cul (19 mg, 0.1 mmol), and N,N-diethyl-2- hydroxybenzamide (39 mg, 0.2 mmol) in dry and degassed DMSO (2 mL) was heated under nitrogen atmosphere at 120 ^ for 20 h. Water (10 mL) is then added and product is extracted to EtOAc (3 x 10 mL). Combined organic layers were washed with water (3 x 10 mL) and brine (2 x 10 mL) and dried over Na ₂ S0 ₄ . After evaporation of the solvent crude product is purified by chromatography to afford title compound: H NMR (CDCI ₃ , 500 MHz) δ 1 .63 (br s, 1 H), 2.33 (s, 3H), 2.37 (s, 3H), 3.02-3.09 (m, 8H), 6.52 (m, 1 H), 6.87 (m, 1 H), 7.04 (m, 1 H), 7.06-7.10 (m, 2H), 7.16 (m, 1 H), 7.39 (d, J= 7.8 Hz, 1 H); MS (ESI) m/z: 299 [MH] ⁺ .

Example 9: Preparation of 1 -(2-((2,4-dimethylphenyl)thio)phenyl)piperazine hydrobromide

(vortioxetine HBr, VII.HBr)

To a solution of vortioxetine VII (1 .80 g, 6.03 mmol) in iPrOAc (20 mL) at room temperature 48% HBr (0.68 mL, 6.03 mmol) was slowly added. Obtained mixture was stirred at room temperature for 1 h, white precipitate was then filtered off, washed with acetone (2 x 20 mL), and dried to afford title compound VII.HBr as a white powder (2.15 g, 94% yield): H NMR (DMSO-d ₆ , 500 MHz) δ 2.23 (s, 3H), 2.32 (s, 3H), 3.15-3.27 (m, 8H), 6.40 (m, 1 H), 6.96 (m, 1 H), 7.08-7.17 (m, 3H), 7.24 (m, 1 H), 7.32 (d, J= 7.8 Hz, 1 H), 8.85 (br, 2H). Reference Example 1 : Preparation of 1 -(2-((2,4-dimethylphenyl)thio)phenyl)piperazine

(vortioxetine, VII)

Mixture of piperazine (1 .0 g, 1 1 .6 mmol), NaOtBu (1 .37 g, 13.8 mmol), Pddba ₂ (40 mg, 0.07 mmol), and 1 ,3-bis(2,6-di-i-propylphenyl)imidazolium chloride (24 mg, 0,07 mmol) in dry and degassed toluene (10 mL) is stirred at room temperature for 1 h. (2,4-Dimethylphenyl)(2- iodophenyl)sulfane V (1 .32 g, 3.86 mmol) is then added, reaction mixture is heated to l OO'C and stirred for 24 h. After cooling to room temperature to the reaction mixture water (5 mL) and Celite (0.4 g) is added. After stirring for 20 min salts are filtered off, organic layer is separated, washed with brine (2 x 10 mL), dried over Na ₂ S0 ₄ and solvent is evaporated to afford crude product, which is then purified by chromatography to afford title compound as yellowish crystals: H NMR (CDCI ₃ , 500 MHz) δ 1 .63 (br s, 1 H), 2.33 (s, 3H), 2.37 (s, 3H),

Reference Example 2: Preparation of 1 -(2-((2,4-dimethylphenyl)thio)phenyl)piperazine

(vortioxetine, VII)

Mixture of piperazine (1 .29 g, 15.0 mmol), NaOtBu (1 .77 g, 17.8 mmol), Pddba2 (52 mg, 0.09 mmol), and rac-BINAP (93 mg, 0,15 mmol) in dry and degassed toluene (10 mL) was stirred at room temperature for 1 h. (2,4-Dimethylphenyl)(2-iodophenyl)sulfane V (1 .70 g, 5.0 mmol) was then added, reaction mixture was heated to 100°C and stirred for 24 h. After cooled to room temperature to the reaction mixture water (5 mL) and Celite (0.4 g) were added. After stirring for 20 min salts were filtered off, organic layer was separated, washed with brine (2 x 10 mL), dried over Na ₂ S0 ₄ and solvent was evaporated to afford product as an orange oil (1 .41 g, 95% yield): H NMR (CDCI ₃ , 500 MHz) δ 1 .63 (br s, 1 H), 2.33 (s, 3H), 2.37 (s, 3H), 3.02-3.09 (m, 8H), 6.52 (m, 1 H), 6.87 (m, 1 H), 7.04 (m, 1 H), 7.06-7.10 (m, 2H), 7.16 (m, 1 H), 7.39 (d, J = 7.8 Hz, 1 H); MS (ESI) m/z: 299 [MH] ⁺ . Comparative Example 1 : Preparation of 1 -(2-((2,4-dimethylphenyl)thio)phenyl)piperazine

(vortioxetine, VII)

Mixture of (2,4-dimethylphenyl)(2-bromohenyl)sulfane V" (0.29 g, 1 .0 mmol), piperazine VI (0.13 g, 1 .5 mmol), K ₃ P0 ₃ (0.42 g, 2.0 mmol), Cul (19 mg, 0.1 mmol), and 2-phenylphenol (68 mg, 0.4 mmol) in dry and degassed DMSO (2 mL) was heated under nitrogen atmosphere at 120°C for 20 h. Vortioxetine VII was not formed.

Comparative Example 2: Preparation of 1 -(2-((2,4-dimethylphenyl)thio)phenyl)piperazine

(vortioxetine, VII)

Mixture of (2,4-dimethylphenyl)(2-bromophenyl)sulfane V (0.29 g, 1 .0 mmol), piperazine VI (0.13 g, 1 .5 mmol), K ₃ P0 ₃ (0.42 g, 2.0 mmol), Cul (19 mg, 0.1 mmol), and N,N-diethyl-2- hydroxybenzamide (39 mg, 0.2 mmol) in dry and degassed DMSO (2 mL) was heated under nitrogen atmosphere at 120 ^ for 20 h. Vortioxetine VII was not formed.