SYNTHETIC LIGATION REASSEMBLY IN DIRECTED EVOLUTION

Title:

SYNTHETIC LIGATION REASSEMBLY IN DIRECTED EVOLUTION

Document Type and Number:

WIPO Patent Application WO2000077262

Kind Code:

A9

Abstract:

The invention is directed to a process of preparing nucleic acid and poly nucleotide libraries by cloning non-randomly generated nucleotide fragments having ligatable ends into an expression vector. The expression of the polynucleotide provides an indirect means by which to screen the polynucleotide library.

Inventors:

SHORT JAY M (US)

Application Number:

PCT/US2000/016838

Publication Date:

June 13, 2002

Filing Date:

June 14, 2000

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Assignee:

DIVERSA CORP (US)
SHORT JAY M (US)

International Classes:

C12N15/09; A61K38/00; A61K39/00; A61K39/39; A61K48/00; A61P33/06; C07K14/445; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/14; C12N15/01; C12N15/10; C12P21/02; C12Q1/02; C12Q1/68; C40B30/00; C40B40/02; C40B50/06; C40B60/12; G01N33/15; G01N33/50; G01N33/53; G01N33/566; C12N; (IPC1-7): C12Q1/68

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