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Title:
SYSTEM FOR IMPLEMENTING HIGH-THROUGHPUT INTEGRATED MICRODROPLET DIGITAL PCR
Document Type and Number:
WIPO Patent Application WO/2023/015758
Kind Code:
A1
Abstract:
Provided is a system for implementing high-throughput integrated microdroplet digital PCR, belonging to the technical field of nucleic acid detection and analysis. The system comprises a microdroplet preparation unit, a PCR amplification unit and a fluorescence detection and analysis unit; the microdroplet preparation unit comprises a driver, a disturbance device and a microdroplet generator; the microdroplet generator comprises an oil phase channel, a sample channel and a detection chamber; the driver generates a drive signal and acts upon the disturbance device; the disturbance device generates a velocity pulse in the oil phase, and then a mixed liquid of an oil-phase shear sample and a PCR reaction reagent, generating microdroplets; the microdroplets are spread uniformly within the detection chamber, and the PCR amplification unit performs a PCR amplification reaction on the microdroplets in the detection chamber; then, the fluorescence detection and analysis unit performs data reading and analysis on the microdroplets so as to complete quantitative detection of the specific nucleic acid. Achieved are a rapid preparation of microdroplets, reduced oil consumption, reduced microdroplet size dependence on the size of a fluid channel, high detection precision and reliability, and low cost.

Inventors:
XIA HUANMING (CN)
XIAO BO (CN)
Application Number:
PCT/CN2021/130781
Publication Date:
February 16, 2023
Filing Date:
November 16, 2021
Export Citation:
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Assignee:
BEIJING HUIZHI MEDICAL INSTR CO LTD (CN)
International Classes:
C12M1/34; C12M1/00; C12M1/02
Foreign References:
CN209307400U2019-08-27
CN104450891A2015-03-25
CN113604344A2021-11-05
CN110945139A2020-03-31
CN111479929A2020-07-31
Attorney, Agent or Firm:
BEIJING ZHONGCHUANG BOTENG INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
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