Title:
SYSTEM AND METHOD FOR CLEAVING ANTIBODIES
Document Type and Number:
WIPO Patent Application WO2004035608
Kind Code:
A3
Abstract:
The present invention is related to a method for producing antibody fragments. In particular, the invention involves a method for the production of F(ab')2 fragments. The method comprises concentration of cell culture media and activation of endogenous enzymes present in the cell culture media by adjusting the temperature and pH.
Inventors:
ZAPATA GERARDO A
Application Number:
PCT/US2003/033121
Publication Date:
August 12, 2004
Filing Date:
October 17, 2003
Export Citation:
Assignee:
ABGENIX INC (US)
International Classes:
C07K16/00; (IPC1-7): C07K16/00; C12P21/06
Other References:
PARHAM P.: "On the fragmentation of monoclonal IgG1, IgG2a, and IgG2b from BALB/c mice", THE JOURNAL OF IMMUNOLOGY, vol. 131, no. 6, December 1983 (1983-12-01), pages 2895 - 2902, XP002014406
JONES R.G.A. ET AL: "Enhanced pepsin digestion: a novel process for purifying antibody F(ab)2 fragments in high yield from serum", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 263, 2002, pages 57 - 74, XP004354385
JONES R.G.A. ET AL: "Enhanced pepsin digestion: a novel process for purifying antibody F(ab)2 fragments in high yield from serum", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 263, 2002, pages 57 - 74, XP004354385
Download PDF:
Previous Patent: HUMAN MONOCLONAL ANTIBODIES AGAINST CD20
Next Patent: USE OF POLY-ALPHA2,8-SIALIC ACID MIMETIC PEPTIDES TO MODULATE NCAM FUNCTIONS.
Next Patent: USE OF POLY-ALPHA2,8-SIALIC ACID MIMETIC PEPTIDES TO MODULATE NCAM FUNCTIONS.