Title:
SYSTEM AND METHOD FOR GENOME EDITING
Document Type and Number:
WIPO Patent Application WO/2018/188571
Kind Code:
A1
Abstract:
A system and method for eukaryotic genome editing, a CRISPR-Cpf1 system capable of highly efficient editing of a eukaryotic genome, and uses of the system.
Inventors:
LI WEI (CN)
ZHOU QI (CN)
TENG FEI (CN)
ZHOU QI (CN)
TENG FEI (CN)
Application Number:
PCT/CN2018/082446
Publication Date:
October 18, 2018
Filing Date:
April 10, 2018
Export Citation:
Assignee:
INST ZOOLOGY CAS (CN)
International Classes:
C12N9/22; A61K48/00; C12N15/10; C12N15/11; C12N15/113; C12N15/63; C12N15/90
Domestic Patent References:
| WO2016186946A1 | 2016-11-24 |
Foreign References:
| CN105907785A | 2016-08-31 | |||
| CN201480045703A | 2014-06-10 |
Other References:
DATABASE Protein 9 February 2016 (2016-02-09), "type V CRISPR-associated protein Cpfl [[Eubacterium] rectale", XP055613736, retrieved from NCBI Database accession no. WP_055272206
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Arcobacter butzleri", XP055613739, retrieved from NCBI Database accession no. WP_052943011. 1
DATABASE Protein 13 November 2015 (2015-11-13), HARWICH, M. D. JR.: "hypothetical protein VC03_02970 [Sneathia amnii", XP055613810, retrieved from NCBI Database accession no. AKC95493. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Helcococcus kunzii", XP055613813, retrieved from NCBI Database accession no. WP_005398606. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Bacteroidetes oral taxon 274", XP055613818, retrieved from NCBI Database accession no. WP_009217842. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Oribacterium sp. NK2B42", XP055613820, retrieved from NCBI Database accession no. WP_049895985. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Butyrivibrio sp. NC3005", XP055613822, retrieved from NCBI Database accession no. WP_035798880. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Proteocatella sphenisci", retrieved from NCBI Database accession no. WP_028830240. 1
DATABASE Protein 7 May 2015 (2015-05-07), BROWN, C. T.: "hypothetical protein US 52_C0007G0008 [candidate division WS6 bacterium GW2011_GWA2_37_6", XP055613829, retrieved from NCBI Database accession no. KKQ36153. 1
DATABASE Protein 17 October 2016 (2016-10-17), VARGHESE, N.: "CRISPR-associated protein Cpfl, subtype PREFRAN [Pseudobu- tyrivibrio xylanivorans", XP055613833, retrieved from NCBI Database accession no. SCZ76797. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Pseudobutyrivibrio ruminis", XP055613837, retrieved from NCBI Database accession no. WP_028248456. 1
GAO P.: "Type V CRISPR-Cas Cpfl endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition", CELL RESEARCH, vol. 26, no. 8, 22 July 2016 (2016-07-22), pages 901 - 913, XP055366082
SAMBROOK, J.FRITSCH, E.F.MANIATIS, T.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
CARRILLO, H.LIPMAN, D., SIAM J APPLIED MATH, vol. 48, 1988, pages 1073
"Biocomputing: Informatics and Genome Projects", 1993, ACADEMIC PRESS
"Computer Analysis of Sequence Data", 1994, HUMANA PRESS
WATSON ET AL.: "Sequence Analysis in Molecular Biology", 1987, THE BENJAMIN/CUMMINGS PUB. CO., pages: 224
"Sequence Analysis Primer", 1991, M STOCKTON PRESS
NAKAMURA Y.: "Codon usage tabulated from the international DNA sequence databases: status for the year 2000", NUCL.ACIDS RES, vol. 28, 2000, pages 292, XP002941557, DOI: 10.1093/nar/28.1.292
ZHANG ET AL., CELL, vol. 163, 22 October 2015 (2015-10-22), pages 1 - 13
LI, W. ET AL.: "Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems", NAT BIOTECHNOL, vol. 31, no. 8, 2013, pages 684 - 6, XP055324100, DOI: 10.1038/nbt.2652
CONG, L. ET AL.: "Multiplex genome engineering using CRISPR/Cas systems", SCIENCE, vol. 339, no. 6121, 2013, pages 819 - 23, XP055458249, DOI: 10.1126/science.1231143
ZETSCHE, B. ET AL.: "Cpfl is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system", CELL, vol. 163, no. 3, 2015, pages 759 - 71
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Arcobacter butzleri", XP055613739, retrieved from NCBI Database accession no. WP_052943011. 1
DATABASE Protein 13 November 2015 (2015-11-13), HARWICH, M. D. JR.: "hypothetical protein VC03_02970 [Sneathia amnii", XP055613810, retrieved from NCBI Database accession no. AKC95493. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Helcococcus kunzii", XP055613813, retrieved from NCBI Database accession no. WP_005398606. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Bacteroidetes oral taxon 274", XP055613818, retrieved from NCBI Database accession no. WP_009217842. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Oribacterium sp. NK2B42", XP055613820, retrieved from NCBI Database accession no. WP_049895985. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Butyrivibrio sp. NC3005", XP055613822, retrieved from NCBI Database accession no. WP_035798880. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Proteocatella sphenisci", retrieved from NCBI Database accession no. WP_028830240. 1
DATABASE Protein 7 May 2015 (2015-05-07), BROWN, C. T.: "hypothetical protein US 52_C0007G0008 [candidate division WS6 bacterium GW2011_GWA2_37_6", XP055613829, retrieved from NCBI Database accession no. KKQ36153. 1
DATABASE Protein 17 October 2016 (2016-10-17), VARGHESE, N.: "CRISPR-associated protein Cpfl, subtype PREFRAN [Pseudobu- tyrivibrio xylanivorans", XP055613833, retrieved from NCBI Database accession no. SCZ76797. 1
DATABASE Protein 1 October 2015 (2015-10-01), "type V CRISPR-associated protein Cpfl [Pseudobutyrivibrio ruminis", XP055613837, retrieved from NCBI Database accession no. WP_028248456. 1
GAO P.: "Type V CRISPR-Cas Cpfl endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition", CELL RESEARCH, vol. 26, no. 8, 22 July 2016 (2016-07-22), pages 901 - 913, XP055366082
SAMBROOK, J.FRITSCH, E.F.MANIATIS, T.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
CARRILLO, H.LIPMAN, D., SIAM J APPLIED MATH, vol. 48, 1988, pages 1073
"Biocomputing: Informatics and Genome Projects", 1993, ACADEMIC PRESS
"Computer Analysis of Sequence Data", 1994, HUMANA PRESS
WATSON ET AL.: "Sequence Analysis in Molecular Biology", 1987, THE BENJAMIN/CUMMINGS PUB. CO., pages: 224
"Sequence Analysis Primer", 1991, M STOCKTON PRESS
NAKAMURA Y.: "Codon usage tabulated from the international DNA sequence databases: status for the year 2000", NUCL.ACIDS RES, vol. 28, 2000, pages 292, XP002941557, DOI: 10.1093/nar/28.1.292
ZHANG ET AL., CELL, vol. 163, 22 October 2015 (2015-10-22), pages 1 - 13
LI, W. ET AL.: "Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems", NAT BIOTECHNOL, vol. 31, no. 8, 2013, pages 684 - 6, XP055324100, DOI: 10.1038/nbt.2652
CONG, L. ET AL.: "Multiplex genome engineering using CRISPR/Cas systems", SCIENCE, vol. 339, no. 6121, 2013, pages 819 - 23, XP055458249, DOI: 10.1126/science.1231143
ZETSCHE, B. ET AL.: "Cpfl is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system", CELL, vol. 163, no. 3, 2015, pages 759 - 71
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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