Title:
SYSTEM AND METHODS FOR DUPLICATING TARGET FRAGMENTS
Document Type and Number:
WIPO Patent Application WO/2023/232024
Kind Code:
A1
Abstract:
Provided are compositions and methods useful for duplicating/amplifying a target fragment on a target DNA sequence such as a genome sequence. The editing system employs a pair of pegRNA which, by virtue of their targeting sites flanking the target fragment, extend the target fragment with reverse transcriptase (RT) templates included in the pegRNA. As the two RT templates at least include portions that are complementary to each other, they can form a duplex region which can then serve as starting point for DNA polymerase to synthesize a new strand for each strand of the target fragment, thereby duplicating the target fragment. Continue this process introduce amplification of this targeted sequence. Alternatively, this process can be done by combination of pegRNA/sgRNA or sgRNA/sgRNA. In the case of sgRNA/sgRNA in a PAM-out position, the RT enzyme and templates are not required.
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Inventors:
YIN HAO (CN)
ZHANG YING (CN)
ZHANG RUIWEN (CN)
HE ZHOU (CN)
ZHANG YING (CN)
ZHANG RUIWEN (CN)
HE ZHOU (CN)
Application Number:
PCT/CN2023/097095
Publication Date:
December 07, 2023
Filing Date:
May 30, 2023
Export Citation:
Assignee:
UNIV WUHAN (CN)
International Classes:
C12N9/12; C12N9/22; C12N15/10; C12N15/11
Domestic Patent References:
| WO2021226558A1 | 2021-11-11 | |||
| WO2022067130A2 | 2022-03-31 | |||
| WO2021165508A1 | 2021-08-26 |
Foreign References:
| CN114127285A | 2022-03-01 | |||
| CN113891937A | 2022-01-04 | |||
| CN113913405A | 2022-01-11 | |||
| CN113874501A | 2021-12-31 |
Attorney, Agent or Firm:
WINGUAN PATENT AND TRADEMARK ATTORNEYS et al. (CN)
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