Title:
SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS
Document Type and Number:
WIPO Patent Application WO/2012/139125
Kind Code:
A3
Abstract:
An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
Inventors:
SCHULTZ JONATHAN (US)
NOBILE JOHN (US)
REED BRIAN (US)
THWAR PRASANNA (US)
ROSWECH TODD (US)
NOBILE JOHN (US)
REED BRIAN (US)
THWAR PRASANNA (US)
ROSWECH TODD (US)
Application Number:
PCT/US2012/032786
Publication Date:
November 22, 2012
Filing Date:
April 09, 2012
Export Citation:
Assignee:
LIFE TECHNOLOGIES CORP (US)
SCHULTZ JONATHAN (US)
NOBILE JOHN (US)
REED BRIAN (US)
THWAR PRASANNA (US)
ROSWECH TODD (US)
SCHULTZ JONATHAN (US)
NOBILE JOHN (US)
REED BRIAN (US)
THWAR PRASANNA (US)
ROSWECH TODD (US)
International Classes:
B01F3/08; B01F5/04; B01F13/00; B01L3/00; B01L7/00; B04B5/04; B04B7/02; C12Q1/68
Domestic Patent References:
| WO2008144288A1 | 2008-11-27 |
Foreign References:
| JP2005279458A | 2005-10-13 |
Attorney, Agent or Firm:
SCHELL, John (5791 Van Allen WayCarlsbad, California, US)
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