Title:
SYSTEMS, METHODS, AND APPARATUS FOR IN VITRO SINGLE-CELL IDENTIFICATION AND RECOVERY
Document Type and Number:
WIPO Patent Application WO/2016/115537
Kind Code:
A3
Abstract:
Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.
Inventors:
ADALSTEINSSON VIKTOR A (US)
LOGINOV DENIS (US)
LOVE J CHRISTOPHER (US)
STOCKDALE ALAN (US)
GIERAHN TODD (US)
LOGINOV DENIS (US)
LOVE J CHRISTOPHER (US)
STOCKDALE ALAN (US)
GIERAHN TODD (US)
Application Number:
PCT/US2016/013724
Publication Date:
September 09, 2016
Filing Date:
January 15, 2016
Export Citation:
Assignee:
MASSACHUSETTS INST TECHNOLOGY (US)
International Classes:
B01L3/00
Domestic Patent References:
| WO2009063462A2 | 2009-05-22 |
Foreign References:
| US20060013031A1 | 2006-01-19 | |||
| EP2170514A1 | 2010-04-07 |
Other References:
MATEU PLA-ROCA ET AL: "Addressable Nanowell Arrays Formed Using Reversibly Sealable Hybrid Elastomer-Metal Stencils", ANALYTICAL CHEMISTRY, vol. 82, no. 9, 1 May 2010 (2010-05-01), pages 3848 - 3855, XP055266929, ISSN: 0003-2700, DOI: 10.1021/ac100335d
Attorney, Agent or Firm:
HAULBROOK, William R. et al. (Hall & Stewart LLPTwo International Plac, Boston Massachusetts, US)
Download PDF: