SENSITIVITY CHECKS FOR DETECTORS

PRIORITY

[0001] This application claims the benefit of priority to U.S. Patent Application No. 15/388,589 filed on December 22, 2016, of the same title, which is hereby incorporated by reference herein in its entirety.

FIELD

[0002] The embodiments described herein relate generally to detector calibration, and, more particularly, to calibration, calibration verification, and sensitivity checks for a detector. More specifically, the methods and systems include calibrating a detector by releasing a calibrant from a calibrant chamber in flow communication with the detector. The systems and methods further include verifying the calibration by releasing a verification substance from a verification chamber in flow communication with the detector. The systems and methods further include checking a sensitivity of the detector by releasing a sensitivity substance from a sensitivity chamber in flow communication with the detector.

BACKGROUND

[0003] Mobility spectrometers measure the intensity of ions arriving at a detector as a function of their drift time in order to characterize a sample. Because this drift time is dependent on experimental conditions, such as temperature and pressure, a drift time axis of the detector needs to be calibrated prior to sample analysis. When a detector is calibrated, absolute drift times are converted into "calibrated units," such that peak positions of detected ions are compared to a library entry. Verification of the calibration may be performed by introducing a sample including a known verification substance into the detector, and confirming that the calibrated units enable accurate characterization of the known verification substance. In addition, a sensitivity check to verify that the detector is configured to detect a minimum amount of a substance may be performed. The sensitivity check may be performed by introducing a sample including a known amount of a known sensitivity substance into the detector, and confirming that the detector detects the known sensitivity substance.

[0004] In at least some known systems, calibration of the detector is performed using external calibrant traps, wherein the calibrant is introduced into the detector through a desorber. These calibration traps, however, have limited lifetimes, can get contaminated, and can be costly to replace and/or refill. Moreover, these calibration processes with external traps are labor-intensive and are not easily automated. Likewise, verification of the calibration and the sensitivity checks may be performed using external traps containing the verification and sensitivity substances, respectively. These external traps face the same drawbacks described above.

[0005] Accordingly, having a system that solves and/ or eliminates these issues would be desirable.

SUMMARY

[0006] The following embodiments and aspects thereof are described and illustrated in conjunction with systems, tools and methods, which are meant to be exemplary and illustrative, and not limiting in scope. The present application discloses numerous embodiments.

[0007] In some embodiments of the present disclosure, a method for internal calibration and verification of a detector is disclosed. The method includes calibrating the detector, wherein the calibration includes releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector, and verifying the calibration, wherein the verification includes releasing at least one verification substance from at least one verification chamber in flow communication with the detector.

[0008] In another embodiment of the present disclosure, a method for internal calibration, verification, and sensitivity checking of a detector is disclosed. The method includes calibrating the detector, wherein the calibration includes releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector, verifying the calibration, wherein the verification includes releasing at least one verification substance from at least one verification chamber in flow communication with the detector, and checking the sensitivity of the detector, wherein the sensitivity checking includes releasing at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector.

[0009] In yet another embodiment of the present disclosure, a method for internal calibration and sensitivity checking of a detector is disclosed. The method includes calibrating the detector, wherein the calibration includes releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector, and checking the sensitivity of the detector, wherein the sensitivity checking includes releasing at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector.

[0010] In another embodiment of the present disclosure, a method for external calibration, internal verification, and internal sensitivity checking of a detector is disclosed. The method includes calibrating the detector, verifying the calibration, and checking the sensitivity of the detector. The verification includes releasing at least one verification substance from at least one verification chamber in flow communication with the detector, and the sensitivity checking includes releasing at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector.

[0011] In still another embodiment of the present disclosure, a system for internal calibration and verification of a detector is disclosed. The system includes at least one detector including at least one detector inlet, at least one calibrant chamber coupled in flow communication with the at least one detector inlet, and at least one verification chamber coupled in flow communication with the at least one detector inlet. The at least one calibrant chamber includes at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet. The at least one verification chamber includes at least one verification substance and at least one verification control valve configured to release an amount of the at least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector.

[0012] In another embodiment of the present disclosure, a system for internal calibration, verification, and sensitivity checking of a detector is disclosed. The system includes at least one detector including at least one detector inlet, at least one calibrant chamber coupled in flow communication with the at least one detector inlet, at least one verification chamber coupled in flow communication with the at least one detector inlet, and at least one sensitivity chamber coupled in flow communication with the at least one detector inlet. The at least one calibrant chamber includes at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet. The at least one verification chamber includes at least one verification substance and at least one verification control valve configured to release an amount of the at least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector. The at least one sensitivity chamber includes at least one sensitivity substance and at least one sensitivity control valve configured to release an amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector.

[0013] In yet another embodiment of the present disclosure, a system for internal calibration and sensitivity checking of a detector is disclosed. The system includes at least one detector including at least one detector inlet, at least one calibrant chamber coupled in flow communication with the at least one detector inlet, and at least one sensitivity chamber coupled in flow communication with the at least one detector inlet. The at least one calibrant chamber includes at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet. The at least one sensitivity chamber includes at least one sensitivity substance and at least one sensitivity control valve configured to release an amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector.

[0014] In a further embodiment of the present disclosure, a system for external calibration, internal verification, and internal sensitivity checking of a detector is disclosed. The system includes at least one detector including at least one detector inlet, at least one calibration component configured to calibrate the at least one detector, at least one verification chamber coupled in flow communication with the at least one detector inlet, and at least one sensitivity chamber coupled in flow communication with the at least one detector inlet. The at least one verification chamber includes at least one verification substance and at least one verification control valve configured to release an amount of the at least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector. The at least one sensitivity chamber includes at least one sensitivity substance and at least one sensitivity control valve configured to release an amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector.

[0015] In some embodiments, the present specification discloses a method for internal calibration and verification of a detector, the method comprising: calibrating the detector, wherein the calibration comprises releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector; and, verifying the calibration, wherein the verification comprises releasing at least one verification substance from at least one verification chamber in flow communication with the detector.

[0016] Optionally, the calibration comprises: ionizing the at least one calibrant; and recording at least one mobility spectrum of the at least one ionized calibrant, wherein the at least one mobility spectrum includes at least one peak.

[0017] Optionally, the calibration comprises adjusting a drift time of the at least one peak.

[0018] Optionally, the method further comprises recording the adjusted drift time in calibrated units.

[0019] Optionally, the verification comprises ionizing the at least one verification substance; automatically adjusting at least one mobility spectrum of the at least one ionized verification substance by at least a portion of a calibrated unit according to the calibration of the detector wherein the at least one mobility spectrum includes at least one peak; and recording the at least one mobility spectrum of the at least one ionized verification substance. Still optionally, the verification further comprises: verifying an adjusted drift time of the at least one peak; and recording the adjusted drift time. Still optionally, the verification of the adjusted drift time comprises comparing the at least one adjusted mobility spectrum to at least one pre-determined mobility spectrum of the at least one verification substance.

[0020] In some embodiments, the present specification discloses a method for internal calibration, verification, and sensitivity checking of a detector, the method comprising: calibrating the detector, wherein the calibration comprises releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector; verifying the calibration, wherein the verification comprises releasing at least one verification substance from at least one verification chamber in flow communication with the detector; and, checking the sensitivity of the detector, wherein the sensitivity checking comprises releasing at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector.

[0021] Optionally, releasing the at least one sensitivity substance comprises releasing a predetermined quantity of the at least one sensitivity substance.

[0022] Optionally, checking the sensitivity comprises: ionizing the at least one sensitivity substance; and recording at least one mobility spectrum of the at least one ionized sensitivity substance, wherein the at least one mobility spectrum includes at least one peak.

[0023] Optionally, checking the sensitivity further comprises comparing the at least one mobility spectrum to at least one pre-determined mobility spectrum of the at least one sensitivity substance.

[0024] Optionally, checking the sensitivity further comprises comparing at least one intensity of the at least one mobility spectrum to a threshold intensity of at least one predetermined mobility spectrum of the at least one sensitivity substance.

[0025] Optionally, the verification comprises: ionizing the at least one verification substance; automatically adjusting at least one at least one mobility spectrum of the at least one ionized verification substance by at least a portion of a calibrated unit according to the calibration of the detector wherein the at least one mobility spectrum includes at least one peak; and recording the at least one mobility spectrum of the at least one ionized verification substance. Optionally, the verification further comprises: verifying an adjusted drift time of the at least one peak; and recording the adjusted drift time. Still optionally, the verification of the adjusted drift time comprises comparing the at least one adjusted mobility spectrum to at least one pre-determined mobility spectrum of the at least one verification substance.

[0026] Optionally, the calibration comprises: ionizing the at least one calibrant; and recording at least one mobility spectrum of the at least one ionized calibrant, wherein the at least one mobility spectrum includes at least one peak. Optionally, the calibration further comprises adjusting a drift time of the at least one peak. Optionally, the method further comprises recording the adjusted drift time in calibrated units.

[0027] In some embodiments, the present specification discloses a method for internal calibration and sensitivity checking of a detector, the method comprising: calibrating the detector, wherein the calibration comprises releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector; and, checking the sensitivity of the detector, wherein the sensitivity checking comprises releasing at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector.

[0028] Optionally, the calibration comprises: ionizing the at least one calibrant; and recording at least one mobility spectrum of the at least one ionized calibrant, wherein the at least one mobility spectrum includes at least one peak. Optionally, the calibration further comprises adjusting a drift time of the at least one peak. Optionally, the method further comprises recording the adjusted drift time in calibrated units.

[0029] Optionally, releasing the at least one sensitivity substance comprises a predetermined quantity of the at least one sensitivity substance.

[0030] Optionally, checking the sensitivity comprises: ionizing the at least one sensitivity substance; and recording at least one mobility spectrum of the at least one ionized sensitivity substance, wherein the at least one mobility spectrum includes at least one peak.

[0031] Optionally, checking the sensitivity further comprises comparing the at least one mobility spectrum to at least one pre-determined mobility spectrum of the at least one sensitivity substance.

[0032] Optionally, checking the sensitivity further comprises comparing at least an intensity of the at least one mobility spectrum to a threshold intensity of at least one predetermined mobility spectrum of the at least one sensitivity substance.

[0033] In some embodiments, the present specification discloses a method for external calibration, internal verification, and internal sensitivity checking of a detector, the method comprising: calibrating the detector; verifying the calibration, wherein the verification comprises releasing at least one verification substance from at least one verification chamber in flow communication with the detector; and checking the sensitivity of the detector, wherein the sensitivity checking comprises releasing at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector.

[0034] Optionally, releasing the at least one sensitivity substance comprises releasing a predetermined quantity of the at least one sensitivity substance.

[0035] Optionally, checking the sensitivity comprises: ionizing the at least one sensitivity substance; and recording at least one mobility spectrum of the at least one ionized sensitivity substance, wherein the at least one mobility spectrum includes at least one peak.

[0036] Optionally, checking the sensitivity further comprises comparing the at least one mobility spectrum to at least one pre-determined mobility spectrum of the at least one sensitivity substance. Still optionally, checking the sensitivity further comprises comparing at least an intensity of the at least one mobility spectrum to a threshold intensity of at least one predetermined mobility spectrum of the at least one sensitivity substance.

[0037] Optionally, the verification comprises: ionizing the at least one verification substance; automatically adjusting at least one mobility spectrum of the at least one ionized verification substance by at least a portion of a calibrated unit according to the calibration of the detector, wherein the at least one mobility spectrum includes at least one peak; and recording the at least one mobility spectrum of the at least one ionized verification substance. Still optionally, the verification further comprises: verifying an adjusted drift time of the at least one peak; and recording the adjusted drift time. Optionally, the verification of the adjusted drift time comprises comparing the at least one adjusted mobility spectrum to at least one pre-determined mobility spectrum of the at least one verification substance.

[0038] In some embodiments, the present specification discloses a system for internal calibration and verification of a detector, the system comprising: at least one detector including at least one detector inlet; at least one calibrant chamber coupled in flow communication with the at least one detector inlet, the at least one calibrant chamber including at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet; and, at least one verification chamber coupled in flow communication with the at least one detector inlet, the at least one verification chamber including at least one verification substance and at least one verification control valve configured to release an amount of the at least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector.

[0039] In some embodiments, the present specification discloses a system for internal calibration, verification, and sensitivity checking of a detector, the system comprising: at least one detector including at least one detector inlet; at least one calibrant chamber coupled in flow communication with the at least one detector inlet, the at least one calibrant chamber including at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet; at least one verification chamber coupled in flow communication with the at least one detector inlet, the at least one verification chamber including at least one verification substance and at least one verification control valve configured to release an amount of the at least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector; and, at least one sensitivity chamber coupled in flow communication with the at least one detector inlet, the at least one sensitivity chamber including at least one sensitivity substance and at least one sensitivity control valve configured to release an amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector.

[0040] In some embodiments, the present specification discloses a system for internal calibration and sensitivity checking of a detector, the system comprising: at least one detector including at least one detector inlet; at least one calibrant chamber coupled in flow communication with the at least one detector inlet, the at least one calibrant chamber including at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet; and, at least one sensitivity chamber coupled in flow communication with the at least one detector inlet, the at least one sensitivity chamber including at least one sensitivity substance and at least one sensitivity control valve configured to release an amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector.

[0041] In some embodiments, the present specification discloses a system for external calibration, internal verification, and internal sensitivity checking of a detector, the system comprising: at least one detector including at least one detector inlet; at least one calibration component configured to calibrate the at least one detector; at least one verification chamber coupled in flow communication with the at least one detector inlet, the at least one verification chamber including at least one verification substance and at least one verification control valve configured to release an amount of the at least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector; and, at least one sensitivity chamber coupled in flow communication with the at least one detector inlet, the at least one sensitivity chamber including at least one sensitivity substance and at least one sensitivity control valve configured to release an amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector.

[0042] In some embodiments, the present specification discloses a method for internal calibration and verification of a detector, the method comprising: calibrating the detector, wherein the calibration comprises releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector; and, verifying the calibration, wherein the verification comprises releasing at least one verification substance from at least one verification chamber in flow communication with the detector, wherein the at least one verification substance has at least one predefined mobility spectrum and wherein the at least one verification chamber and at least one calibrant chamber are separate chambers.

[0043] In some embodiments, the present specification discloses a method for internal calibration, verification, and sensitivity checking of a detector, the method comprising: calibrating the detector, wherein the calibration comprises releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector; verifying the calibration, wherein the verification comprises releasing at least one verification substance from at least one verification chamber in flow communication with the detector, wherein the at least one verification substance has at least one predefined mobility spectrum and wherein the at least one verification chamber and at least one calibrant chamber are separate chambers; and, checking the sensitivity of the detector, wherein the sensitivity checking comprises releasing at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector. [0044] In some embodiments, the present specification discloses a method for internal calibration and sensitivity checking of a detector, the method comprising: calibrating the detector, wherein the calibration comprises releasing at least one calibrant from at least one calibrant chamber in flow communication with the detector; and, checking the sensitivity of the detector, wherein the sensitivity checking comprises releasing a known amount of at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector and wherein the at least one calibrant chamber and at least one sensitivity chamber are separate chambers.

[0045] In some embodiments, the present specification discloses a method for external calibration, internal verification, and internal sensitivity checking of a detector, the method comprising: calibrating the detector; verifying the calibration, wherein the verification comprises releasing at least one verification substance from at least one verification chamber in flow communication with the detector, wherein the at least one verification substance has at least one predefined mobility spectrum; and checking the sensitivity of the detector, wherein the sensitivity checking comprises releasing at least one sensitivity substance from at least one sensitivity chamber in flow communication with the detector, wherein the at least one verification chamber and at least one sensitivity chamber are separate chambers.

[0046] In some embodiments, the present specification discloses a system for internal calibration and verification of a detector, the system comprising: at least one detector including at least one detector inlet; at least one calibrant chamber coupled in flow communication with the at least one detector inlet, the at least one calibrant chamber including at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet; and, at least one verification chamber coupled in flow communication with the at least one detector inlet, the at least one verification chamber including at least one verification substance, wherein the at least one verification substance has at least one predefined mobility spectrum and wherein the at least one verification chamber and at least one calibrant chamber are separate chambers, and at least one verification control valve configured to release an amount of the at least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector. [0047] In some embodiments, the present specification discloses a system for internal calibration, verification, and sensitivity checking of a detector, the system comprising: at least one detector including at least one detector inlet; at least one calibrant chamber coupled in flow communication with the at least one detector inlet, the at least one calibrant chamber including at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet; at least one verification chamber coupled in flow communication with the at least one detector inlet, the at least one verification chamber including at least one verification substance, wherein the at least one verification substance has at least one predefined mobility spectrum, and at least one verification control valve configured to release an amount of the least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector; and, at least one sensitivity chamber coupled in flow communication with the at least one detector inlet, the at least one sensitivity chamber including at least one sensitivity substance and at least one sensitivity control valve configured to release an amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector, wherein the at least one verification chamber, the at least one sensitivity chamber, and the at least one calibrant chamber are separate chambers.

[0048] In some embodiments, the present specification discloses a system for internal calibration and sensitivity checking of a detector, the system comprising: at least one detector including at least one detector inlet; at least one calibrant chamber coupled in flow communication with the at least one detector inlet, the at least one calibrant chamber including at least one calibrant and at least one calibrant control valve configured to release an amount of the at least one calibrant into the at least one detector inlet; and, at least one sensitivity chamber coupled in flow communication with the at least one detector inlet, the at least one sensitivity chamber including a known amount of at least one sensitivity substance and at least one sensitivity control valve configured to release an the known amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector, wherein the at least one calibrant chamber and the at least one sensitivity chamber are separate chambers.

[0049] In some embodiments, the present specification discloses a system for external calibration, internal verification, and internal sensitivity checking of a detector, the system comprising: at least one detector including at least one detector inlet; at least one calibration component configured to calibrate the at least one detector; at least one verification chamber coupled in flow communication with the at least one detector inlet, the at least one verification chamber including at least one verification substance, wherein the at least one verification substance has at least one predefined mobility spectrum, and at least one verification control valve configured to release an amount of the at least one verification substance into the at least one detector inlet and verify the calibration of the at least one detector; and, at least one sensitivity chamber coupled in flow communication with the at least one detector inlet, the at least one sensitivity chamber including at least one sensitivity substance and at least one sensitivity control valve configured to release an amount of the at least one sensitivity substance into the at least one detector inlet and analyze the sensitivity of the at least one detector, wherein the at least one verification chamber and at least one sensitivity chamber are separate chambers.

[0050] In some embodiments, the present specification discloses a system for performing internal calibration, verification and sensitivity checking of a detector, the system comprising: at least one detector including at least one detector inlet; and a chamber coupled in flow communication with the at least one detector inlet, the chamber including first, second and third substances, said chamber configured to release an amount of each of the first, second and third substances into the at least one detector inlet and use the first substance to calibrate, the second substance to verify the calibration and the third substance to analyze the sensitivity of the at least one detector.

[0051] Optionally, the first substance is a calibrant, the second substance is a verification substance and the third substance is a sensitivity checking substance, wherein the calibration comprises ionizing the calibrant and recording at least one mobility spectrum of the ionized calibrant, said at least one mobility spectrum includes at least one peak, wherein the verification comprises verifying an adjusted drift time of the at least one peak and recording the adjusted drift time and wherein the sensitivity checking comprises ionizing the at least one sensitivity substance and recording at least one mobility spectrum of the at least one ionized sensitivity substance, said the at least one mobility spectrum includes at least one peak. [0052] Optionally, the first, second and third substances are present in the chamber as a mixture.

[0053] Optionally, the chamber includes a first permeation tube to contain the first substance, a second permeation tube to contain the second substance and a third permeation tube to contain the third substance.

[0054] Optionally, the chamber includes a first valve to release the first substance, a second valve to release the second substance and a third valve to release the third substance.

[0055] Optionally, the first, second and third substances do not deplete each other's ions or do not deplete any background peaks or the peaks arising from any of the first, second and third substances.

[0056] In some embodiments, the present specification discloses a system for performing first and second operations associated with a detector, the system comprising: at least one detector including at least one detector inlet; and a chamber coupled in flow communication with the at least one detector inlet, the chamber including first and second substances, said chamber configured to release an amount of each of the first and second substances into the at least one detector inlet, wherein the first substance is used to perform the first operation and the second substance is used to perform the second operation.

[0057] Optionally, the first operation is an internal calibration and the second operation is an internal verification of the at least one detector. Optionally, the first substance is a calibrant and the second substance is a verification substance.

[0058] Optionally, the first operation is an internal calibration and the second operation is an internal sensitivity checking of the at least one detector. Optionally, the first substance is a calibrant and the second substance is a sensitivity checking substance.

[0059] Optionally, the first operation is an internal verification and the second operation is an internal sensitivity checking of the at least one detector. Optionally, the first substance is a verification substance and the second substance is a sensitivity checking substance. [0060] Optionally, the first and second substances are present in the chamber as a mixture.

[0061] Optionally, the chamber includes a first permeation tube to contain the first substance and a second permeation tube to contain the second substance.

[0062] Optionally, the chamber includes a first valve to release the first substance and a second valve to release the second substance.

[0063] Optionally, the first and second substances do not deplete each other's ions or do not deplete any background peaks or the peaks arising from any of the first and second substances.

[0064] In some embodiments, the present specification discloses a method for internal calibration, verification, and sensitivity checking of a detector, the method comprising: storing a calibrant, at least one verification substance and at least one sensitivity checking substance in a chamber, said chamber in flow communication with the detector; releasing the calibrant, at least one verification substance and at least one sensitivity checking substance into the detector; calibrating the detector, wherein the calibration comprises ionizing the calibrant and recording at least one mobility spectrum of the ionized calibrant, wherein the at least one mobility spectrum includes at least one peak; verifying the calibration, wherein the verification comprises verifying an adjusted drift time of the at least one peak and recording the adjusted drift time; and, checking the sensitivity of the detector, wherein the sensitivity checking comprises ionizing the at least one sensitivity substance and recording at least one mobility spectrum of the at least one ionized sensitivity substance, wherein the at least one mobility spectrum includes at least one peak.

[0065] In some embodiments, the present specification discloses a method for performing first and second operations associated with a detector, the method comprising: storing first and second substances in a chamber, said chamber in flow communication with the detector; and releasing the first and second substance into the detector, wherein the first substance is used to perform the first operation and the second substance is used to perform the second operation. [0066] Optionally, the first operation is an internal calibration and the second operation is an internal verification of the detector. Optionally, the first substance is a calibrant and the second substance is a verification substance, wherein the calibration comprises ionizing the calibrant and recording at least one mobility spectrum of the ionized calibrant, said at least one mobility spectrum including at least one peak and wherein the verification comprises verifying an adjusted drift time of the at least one peak and recording the adjusted drift time.

[0067] Optionally, the first operation is an internal calibration and the second operation is an internal sensitivity checking of the at least one detector. Optionally, the first substance is a calibrant and the second substance is a sensitivity checking substance, wherein the calibration comprises ionizing the calibrant and recording at least one mobility spectrum of the ionized calibrant, said at least one mobility spectrum including at least one peak, and wherein the sensitivity checking comprises ionizing the at least one sensitivity substance and recording at least one mobility spectrum of the at least one ionized sensitivity substance, said at least one mobility spectrum including at least one peak.

[0068] Optionally, the first operation is an internal verification and the second operation is an internal sensitivity checking of the at least one detector. Optionally, the first substance is a verification substance and the second substance is a sensitivity checking substance, wherein the verification comprises verifying an adjusted drift time of the at least one peak and recording the adjusted drift time, and wherein the sensitivity checking comprises ionizing the at least one sensitivity substance and recording at least one mobility spectrum of the at least one ionized sensitivity substance, said at least one mobility spectrum including at least one peak.

[0069] Optionally, the first and second substances are present in the chamber as a mixture.

[0070] Optionally, the chamber includes a first permeation tube to contain the first substance and a second permeation tube to contain the second substance.

[0071] Optionally, the chamber includes a first valve to release the first substance and a second valve to release the second substance. [0072] Optionally, the first and second substances do not deplete each other's ions or do not deplete any background peaks or the peaks arising from any of the first and second substances.

[0073] The aforementioned and other embodiments of the present specification shall be described in greater depth in the drawings and detailed description provided below.

BRIEF DESCRIPTION OF THE DRAWINGS

[0074] FIG. 1 is a schematic diagram of an exemplary embodiment of a detection system including an internal calibrant chamber in accordance with the present disclosure;

[0075] FIG. 2 is a schematic diagram of an exemplary embodiment of a detection system including an internal calibrant chamber and a verification chamber;

[0076] FIG. 3 is a schematic diagram of an exemplary embodiment of a detection system including an internal calibrant chamber, a verification chamber, and a sensitivity chamber;

[0077] FIG. 4 is an exemplary embodiment of a screen-capture of a calibration process performed by the detection system shown in FIGS. 1-3, in accordance with the present disclosure;

[0078] FIG. 5 is an exemplary embodiment of a screen-capture of another calibration process performed by the detection system shown in FIGS. 1-3, in accordance with the present disclosure;

[0079] FIG. 6 is an exemplary embodiment of a screen-capture of a verification process performed by the detection system shown in FIGS. 2 and 3 to verify the calibration illustrated in FIG. 5, in accordance with the present disclosure;

[0080] FIG. 7 is a schematic diagram of an exemplary embodiment of a detection system including an internal chamber in accordance with the present disclosure; and,

[0081] FIG. 8 is a flow chart illustrating a plurality of steps of a method for internal calibration, verification and sensitivity checking of a detector using a single internal chamber, in accordance with the present disclosure.

DETAILED DESCRIPTION

[0082] The embodiments disclosed herein improve calibration of detectors, verification of the calibration, and checking the sensitivity of the detectors. More specifically, the embodiments disclosed herein provide calibration, verification, and sensitivity check chambers that enable automated calibration, verification, and sensitivity checks. When providing these chambers internally, these processes are less labor-intensive and require smaller sample sizes of the calibration, verification, and sensitivity substances, which, in turn, reduces the cost of these systems in comparison to systems employing external traps. In addition, introducing any substance other than a sample (e.g., including a substance of interest) may constitute a possibility for charge competition between the substance and the sample and/or overlapping peaks in a detected signal that may compromise the response by either reducing the intensity of the target peak or altering its peak position. Using the calibration, verification, and sensitivity check chambers provided herein enables corresponding calibration, verification, and sensitivity checks to be performed quickly, thereby reducing the time any non-substance sample is introduced into the detector. Moreover, in some embodiments, these processes are performed at regular intervals, further reducing the amount of substance needed as well as the processing time.

[0083] In the following specification and the claims, a number of terms are referenced that have the following meanings.

[0084] The singular forms "a", "an", and "the" include plural references unless the context clearly dictates otherwise.

[0085] "Optional" or "optionally" means that the subsequently described event or circumstance may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

[0086] Approximating language, as used herein throughout the specification and claims, may be applied to modify any quantitative representation that could permissibly vary without resulting in a change in the basic function to which it is related. Accordingly, a value modified by a term or terms, such as "about", "approximately", and "substantially", are not to be limited to the precise value specified. In at least some instances, the approximating language may correspond to the precision of an instrument for measuring the value. Here and throughout the specification and claims, range limitations may be combined and/or interchanged. Such ranges are identified and include all the sub-ranges contained therein unless context or language indicates otherwise.

[0087] The term "calibration" refers to the introduction and analysis of a known substance, referred to as a calibration substance or calibrant, into the detection system, either internally or externally, with the goal of associating a certain peak location (drift time in calibrated units) in a mobility spectrum/plasmagram with an absolute drift time, measured in ms, in the detection system. By definition, calibration is focused on determining a drift time dimension, along the mobility axis, as opposed to determining an intensity dimension (peak height), and therefore requires the use of a calibrant for which drift time in calibrated units is already known. Any sample in which the identity of the substance (and the ions generated from it) is not known or defined will not be able to serve the purpose of performing the calibration. Unknown substances are therefore not applicable to the calibration process and do not match the definition of calibration.

[0088] The term "verification" refers to the introduction and analysis of one or more known substances or mixtures of substances, referred to as verification substance(s), into the detection system, either internally or externally, with the goal of verifying an already determined association between a certain peak location (drift time in calibrated units) in a mobility spectrum/plasmagram with an absolute drift time, measured in ms, in the detection system. The already determined association is typically established by the calibration process. The verification substance preferably differs from the calibrant but does not necessarily have to be different from the calibrant. Verification preferably does not change the established association between the calibrated peak location and absolute drift time. By definition, calibration is focused on verifying a drift time dimension, along the mobility axis, and therefore requires the use of a substance for which drift time is already known. Any sample in which the identity of the substance (and the ions generated from it) is not known or defined will not be able to serve the purpose of verifying the calibration. Unknown substances are therefore not applicable to the verification process and do not match the definition of verification.

[0089] The term "sensitivity check" refers to the introduction and analysis of one or more known substances or mixtures of substances, referred to as sensitivity substance(s) or sensitivity check substance(s), into the detection system, either internally or externally, with the goal of evaluating whether the response of the detection system fulfills certain predetermined criteria, such as determining if the intensity of the response at a predetermined peak location meets, exceeds, or falls short of a threshold. The sensitivity check substance preferably differs from the calibrant and verification substance but does not necessarily have to be different from the calibrant or verification substance. The sensitivity check relies on the accuracy of the calibration of the drift time axis and primarily evaluates the intensity dimension of the detector response. By definition, sensitivity check is focused on evaluating the signal intensity, while also evaluating sufficient proximity to a target drift time at which the check is performed and therefore requires the use of a substance for which a signal characteristics are already known. Any sample in which the identity of the substance (and the ions generated from it) is not known or defined will not be able to serve the purpose of performing the sensitivity check. Unknown substances are therefore not applicable to the sensitivity check process and do not match the definition of a sensitivity check substance.

[0090] The present specification is directed towards multiple embodiments. The following disclosure is provided in order to enable a person having ordinary skill in the art to practice the invention. Language used in this specification should not be interpreted as a general disavowal of any one specific embodiment or used to limit the claims beyond the meaning of the terms used therein. The general principles defined herein may be applied to other embodiments and applications without departing from the spirit and scope of the invention. Also, the terminology and phraseology used is for the purpose of describing exemplary embodiments and should not be considered limiting. Thus, the present invention is to be accorded the widest scope encompassing numerous alternatives, modifications and equivalents consistent with the principles and features disclosed. For purpose of clarity, details relating to technical material that is known in the technical fields related to the invention have not been described in detail so as not to unnecessarily obscure the present invention. [0091] In the description and claims of the application, each of the words "comprise" "include" and "have", and forms thereof, are not necessarily limited to members in a list with which the words may be associated. It should be noted herein that any feature or component described in association with a specific embodiment may be used and implemented with any other embodiment unless clearly indicated otherwise.

[0092] The present disclosure is directed to calibrating a detector (either internally or externally), verifying the calibration of the detector, and checking the sensitivity of the detector. In particular, a detection system includes an internal calibrant chamber containing a calibrant. The calibrant is introduced into the detector for detection of the calibrant and calibration of the detector. In some embodiments, the detection system further includes an internal verification chamber containing a verification substance. The verification substance is introduced into the detector for detection of the verification substance and verification of the calibration of the detector. In some embodiments, the detection system also includes an internal sensitivity chamber containing a sensitivity substance. The sensitivity substance is introduced into the detector for detection of the sensitivity substance and to check the sensitivity of the detector.

[0093] In some embodiments, the detector of the present disclosure (also referred to herein as an "analysis device") includes at least one of an ion mobility spectrometer (HVIS), an ion trap mobility spectrometer (ITMS), a drift spectrometer (DS), a non-linear drift spectrometer, a field ion spectrometer (FIS), a radio frequency ion mobility increment spectrometer (EVIIS), a field asymmetric ion mobility spectrometer (FAIMS), an ultra-high-field FAEVIS, a differential ion mobility spectrometer (DEVIS) and a differential mobility spectrometer (DMS), a traveling wave ion mobility spectrometer, a mass spectrometer (MS), a gas chromatograph (GC), and combinations thereof.

[0094] In some embodiments, the method includes using an ionization source to ionize one or more substances (e.g., a sample, calibrant, verification substance, and/or sensitivity substance) within the detector. The ionization source is any ionization system that enables operation of the methods and systems as described herein, including, without limitation, a radioactive ionization source, an electrospray ionization source (ESI), an atmospheric pressure chemical ionization (APCI) source, a corona discharge ionization source, a partial discharge ionization source, an atmospheric pressure photoionization (APPI) source, an atmospheric pressure glow discharge (APGD) source, a direct analysis in real time (DART) source, an atmospheric pressure dielectric barrier discharge (APDBD) source, and an Electron Ionization (EI). In some embodiments of the present disclosure, the ionization source comprises at least one of an APCI source, an APPI source, an ESI source and a DART source. Some embodiments of the present disclosure are configured to operate at sub-atmospheric pressures. Such embodiments include an ionization source that is, without limitation, a chemical ionization (CI) source, a photoionization (PI) source, a glow discharge (GD) source, a dielectric barrier discharge (DBD) source and combinations thereof.

[0095] In some embodiments, a method for internal calibration and verification of a detector is disclosed. The method includes calibrating the detector, wherein the calibration includes releasing at least one calibrant from a calibrant chamber in flow communication with the detector, and verifying the calibration, wherein the verification includes releasing at least one verification substance from a verification chamber in flow communication with the detector.

[0096] In some embodiments, a method for internal calibration, verification, and sensitivity checking of a detector is disclosed. The method includes calibrating the detector, wherein the calibration includes releasing at least one calibrant from a calibrant chamber in flow communication with the detector, verifying the calibration, wherein the verification includes releasing at least one verification substance from a verification chamber in flow communication with the detector, and checking the sensitivity of the detector, wherein the sensitivity checking includes releasing at least one sensitivity substance from a sensitivity chamber in flow communication with the detector.

[0097] In some embodiments, a method for internal calibration and sensitivity checking of a detector is disclosed. The method includes calibrating the detector, wherein the calibration includes releasing at least one calibrant from a calibrant chamber in flow communication with the detector, and checking the sensitivity of the detector, wherein the sensitivity checking includes releasing at least one sensitivity substance from a sensitivity chamber in flow communication with the detector. [0098] FIG. 1 is a schematic diagram of an exemplary embodiment of a detection system 100. In the illustrated embodiment, detection system 100 includes a detector 102, a desorber 104, a sample pump 106, and a calibrant chamber 108. Detection system 100 further includes a nozzle 110 and a filter 112 in serial flow communication with desorber 104 and detector 102.

[0099] Detector 102 is configured to detect and identify constituents in a sample input thereto. For example, in some embodiments, detector 102 is configured to detect one or more substances of interest in a sample gas, such as one or more volatile or non-volatile substances of interest. Detector 102 includes a detector inlet 103 through which substances enter detector 102. It should be understood that where reference is made to a substance entering or flowing into detector 102, those substances enter detector 102 through detector inlet 103.

[00100] Sample pump 106 is in flow communication with calibrant chamber 108 via a valve 120. Sample pump 106 is also in flow communication with detector 102 via valve 120. In the illustrated embodiment, valve 120 includes a three-way valve 120, such that, based on a position of valve 120, gas 114 output from sample pump 106 will flow through valve 120 to calibrant chamber 108, will flow through valve 120 to an exhaust outlet, or will not flow through valve 120.

[00101] When detection system 100 is operating to calibrate detector 102, valve 120 is positioned such that gas from sample pump 106 flows through valve 120 and through calibrant chamber 108. In some embodiments, this gas is referred to as a "carrier gas" 116. Carrier gas 116 includes at least one of ambient air, purified air, dried air, purified nitrogen, and combinations thereof. In the exemplary embodiment, carrier gas 116 has a flow rate (e.g., from sample pump 106) of from about 5 mL/min to about 5000 mL/min, from about 5 mL/min to about 1000 mL/min, from about 10 mL/min to about 100 mL/ min, or from about 25mL/min to about 50mL/min. Carrier gas 116 flows through calibrant chamber 108 to "pick up" the calibrant therein and actively delivers the calibrant to detector 102. The combination of carrier gas 116 and the calibrant carried therein is referred to herein as "calibrant gas" 124. Calibrant chamber 108 is in flow communication with detector 102 via a valve 122. In the illustrated embodiment, valve 122 includes a two-way valve 122, such that, based on a position of valve 122, calibrant gas 124 will flow through valve 122 to detector 102 or will not flow through valve 122. To calibrate detector 102, valve 122 is positioned such that calibrant gas 124 flows through valve 122 and to detector 102 for detection of the calibrant. Having internal calibrant chamber 108 enables detection system 100 to use less calibrant than alternative detection systems employing external traps, as detection system 100 is designed to channel the calibrant gas 124 directly to detector 102. Additionally or alternatively, detection system 100 channels the calibrant gas 124 through desorber 104 to detector 102.

[00102] In the exemplary embodiment, the calibrant includes at least one of Dibenzylamine, Diethylphenylmalonate, Di-isopropylphenol, 2,4-Dimethylpyridine, Dioctylphthalate, Dinitrotoluene, Dipropylene glycol monom ethyl ether, 2,6-Di-t-butylpyridine, Ethyl salicylate, Hexachloroethane, Hexaphenylbenzene, 4-Methyl-2,6-di-tert-butyl phenol, Methyl salicylate, Nicotinamide, 4-Nitrobenzonitrile, 5-Nitrovanillin, Pentachloroethane, Trihexylamine, and combinations thereof. Whatever particular substance the calibrant is, the calibrant is "known" to detector 102 (e.g., to an operator thereof) prior to calibration of detector 102 using the calibrant. Detector 102 compares a detected signal of the calibrant to a "library" of expected signals representative of different substances, including the calibrant. Detector 102 then adjusts the detected signal by at least a portion of a "calibration unit" in order to match the detected signal to the expected signal for the calibrant. It should be understood that the calibration unit is an arbitrary unit of measure specific to a particular calibration, and accordingly, reference to "one," "more than one," "less than one," and/or "a portion of a "calibrated unit" may reference the same amount of absolute time. Subsequent detected signals (e.g., sample signals of unknown substances) are then adjusted by same amount (e.g., the at least a portion of the calibration unit) in order to accurately compare the detected (sample) signals to the library of substances for identification of constituents of a sample.

[00103] In some embodiments, calibrant chamber 108 includes from about 10 mg to about 500 g of the calibrant. In some embodiments, calibrant chamber 108 includes from about 50 mg to about 250 g of the calibrant, from about 25 mg to about 100 g of the calibrant, or from about 20 mg to about 50 g of the calibrant. More specifically, in some embodiments, calibrant chamber 108 includes from about 0.1 g to about 10 g of the calibrant. [00104] In some embodiments, calibrant chamber 108 includes a permeation tube (not specifically shown) including the calibrant. The permeation tube is configured to emit the calibrant at a particular, predetermined rate. In some embodiments, the calibrant is emitted from the permeation tube at an emission rate of from about 0.4 μg/min to about 30 μg/min. In some embodiments, the calibrant is emitted from the permeation tube at an emission rate of from about 0.5 μg/min to about 25 μg/min, from about 0.75 μg/min to about 20 μg/min, or from about 0.9 μg/min to about 10 μg/min. In some embodiments, the calibrant is emitted from the permeation tube at an emission rate of from about 1 μg/min to about 2 μg/min. In other embodiments, calibrant chamber 108 includes a control valve (not specifically shown) configured to release the calibrant at a particular, predetermined rate. In some embodiments, the control valve releases the calibrant at an emission rate of from about 0.4 μg/min to about 30 μg/min. In some embodiments, the control valve releases the calibrant at an emission rate of from about 0.5 μg/min to about 25 μg/min, from about 0.75 μg/min to about 20 μg/min, or from about 0.9 μg/min to about 10 μg/min. In some embodiments, the control valve releases the calibrant at an emission rate of from about 1 μg/min to about 2 μg/min. Carrier gas 116 flows into calibration chamber 108 and "picks up" the calibrant released from the permeation tube and/or by the control valve for analysis by detector 102 and calibration of the detector 102.

[00105] In some embodiments, calibration of detector 102 includes ionization of the calibrant in calibrant gas 124. Detector 102 detects and records at least one mobility spectrum of the ionized calibrant. In some embodiments, detector 102 additionally or alternatively detects and records at least one differential mobility spectrum and/or mass spectrum, depending upon the type of detector 102. Accordingly, where "mobility spectrum" is used herein, it should be understood to refer generally to any of these spectra. In the exemplary embodiment, the mobility spectra are plotted on axes corresponding to a drift time and a "count" or "magnitude". Where other types of spectra are detected, one or more of the axes correspond to other characteristics, such as compensation voltage or mass. Drift time is an inherent characteristic of a substance. Therefore, detecting a drift time of a substance enables identification of that substance. Each mobility spectrum includes at least one peak, wherein a particular peak of the mobility spectrum corresponds to the detected calibrant. Detector 102 identifies the drift time associated with the peak, and adjusts the detected drift time of the peak to a known drift time associated with the calibrant. [00106] In some embodiments, detector 102 accesses a library of "known" or "expected" signals or mobility spectra, each spectrum associated with a particular substance. These known mobility spectra are developed by performing a "base calibration" for each substance in the library. The base calibration includes analyzing a known mobility spectrum for each known substance, or, more specifically, analyzing and identifying at least one peak position of the known substance. The known mobility spectra, including the corresponding peak positions, are stored in the library. Detector 102 identifies the known spectrum for the calibrant and adjusts the detected drift time of the detected calibrant peak to match a known drift time associated with a known calibrant peak. For example, in some embodiments, detector 102 shifts the peak up or down a drift time axis by X ms. Detector 102 records this adjustment (e.g., +X ms) as one or more calibration unit(s).

[00107] In some embodiments, detector 102 is calibrated at fixed intervals. Moreover, in some embodiments, the fixed intervals are pre-determined, for example, by an operator (not shown) of detection system 100 and/or by pre-programming detection system 100. In some embodiments, the fixed interval is between about 10 minutes and about 24 hours, between about 15 minutes and about 8 hours, between about 30 minutes and about 4 hours, between about 1 hour and about 3 hours, about 2 hours, or about 30 minutes. In some embodiments, the fixed interval is "flexible," in that additional calibrations are performed before the fixed interval has fully elapsed. For example, in some embodiments, detection system 100 is pre-programmed to initiate calibration in the event of a pressure change outside of a certain threshold. As another example, detection system 100 is pre-programmed to shorten one or more intervals if a quality of the calibration is low (e.g., low confidence in the calibration results or poor verification). Additionally or alternatively, detector 102 is calibrated "on-demand," at a time determined by a user (e.g., an operator) of detection system 100. For example, in some embodiments, detector 102 is calibrated on a sample-by-sample basis. In some embodiments, detector 102 is calibrated substantially continuously, for example, before and/or after each sample is analyzed.

[00108] In some embodiments, a temperature of calibrant chamber 108 is maintained before, during, and/or after calibration of detector 102. More particularly, in certain embodiments, the temperature of calibrant chamber 108 is maintained between about 0 °C and about 200 °C, between about 10 °C and about 150 °C, between about 15 °C and about 100 °C, or between about 20 °C and about 50°C. The temperature is maintained within calibrant chamber 108 by at least one of heating, cooling, and combinations thereof. For example, in some embodiments, detection system 100 includes one or more heating elements and/or cooling elements (not shown), such as heaters, fans, heating/cooling blocks, and/or any other heating/cooling elements. Additionally or alternatively, the temperature is maintained passively, for example, at an ambient temperature of an environment around detection system 100.

[00109] When detection system 100 is operating to detect and identify one or more unknown constituents of a sample gas, valve 120 is positioned such that gas 114 from sample pump 106 flows through valve 120, to a sample input (not shown), and, in some embodiments, to desorber 104. Desorber 104 (if present) vaporizes the sample and channels the vaporized sample through nozzle 110 and filter 112 to detector 102. Detector 102 analyzes the vaporized sample to identify any substances of interest therein.

[00110] In the illustrated embodiment, detection system 100 further includes a computing device 130. Although computing device 130 is illustrated as remote from detector 102 and in communication therewith, it should be understood that, in some embodiments, computing device 130 is integral to detector 102. In some embodiments, computing device 130 includes a memory device 132 and a processor 134 operatively coupled to memory device 132 for executing instructions. In some embodiments, executable instructions are stored in the memory device 132. Computing device 130 is configurable to perform one or more operations described herein by programming processor 134. For example, in some embodiments, processor 134 is programmed by encoding an operation as one or more executable instructions and providing the executable instructions in memory device 132. In the exemplary embodiment, memory device 132 is one or more devices that enable storage and retrieval of information such as executable instructions and/or other data. Memory device 132 includes one or more computer readable media in some embodiments.

[00111] Memory device 132 is configured to store a pre-programmed library of mobility spectra, each mobility spectrum associated with one substance of a plurality of substances. Memory device 132 may further store associated drift times, alarm limits, detection history, calibration profiles and history (e.g., verification history) for detector 102, and/or any other type data in some embodiments. In the exemplary embodiment, computing device 130, including memory device 132, includes, without limitation, sufficient computer- readable/executable instructions, sufficient data and data structures, algorithms, and commands to facilitate detection of substance(s) introduced into detector 102 (e.g., a sample, the calibrant, the verification substance, and/or the sensitivity substance).

[00112] In the exemplary embodiment, substance detection system 100 further includes an operator presentation and/or control interface 136 coupled to computing device 130. Interface 136 presents data, such as mobility spectra, calibration procedures, verification outcomes, and/or sensitivity check outcomes. In some embodiments, interface 136 includes one or more display devices. In some embodiments, interface 136 presents an audible and/or graphical notification upon detection of a substance of interest. Also, in some embodiments, interface 136 facilitates control of computing device 130 and manual data input into computing device 130. Furthermore, in some embodiments, computing device 130 is coupled in communication with one or more other devices, such as another computing device 130, locally or remotely. As such, in some embodiments, substance detection system 100 is networked with other systems and devices such that data transmitted across portions of system 100 is accessed by any device capable of accessing computing device 130 including, without limitation, desktop computers, laptop computers, and personal digital assistants (PDAs) (neither shown).

[00113] As used herein, the term "computer" and related terms, e.g., "computing device", are not limited to integrated circuits referred to in the art as a computer, but broadly refers to a microcontroller, a microcomputer, a programmable logic controller (PLC), an application specific integrated circuit, and other programmable circuits, and these terms are used interchangeably herein. Further, as used herein, the terms "software" and "firmware" are interchangeable, and include any computer program stored in memory for execution by personal computers, workstations, clients and servers.

[00114] As used herein, the term "non-transitory computer-readable media" is intended to be representative of any tangible computer-based device implemented in any method or technology for short-term and long-term storage of information, such as, computer-readable instructions, data structures, program modules and sub-modules, or other data in any device. Therefore, the methods described herein may be encoded as executable instructions embodied in a tangible, non-transitory, computer readable medium, including, without limitation, a storage device and/or a memory device. Such instructions, when executed by a processor, cause the processor to perform at least a portion of the methods described herein. Moreover, as used herein, the term "non-transitory computer-readable media" includes all tangible, computer- readable media, including, without limitation, non-transitory computer storage devices, including, without limitation, volatile and nonvolatile media, and removable and non-removable media such as a firmware, physical and virtual storage, CD-ROMs, DVDs, and any other digital source such as a network or the Internet, as well as yet to be developed digital means, with the sole exception being a transitory, propagating signal.

[00115] FIG. 2 is a schematic diagram of another exemplary embodiment of detection system 100, as shown in FIG. 1. More particularly, detection system 100 is shown to further include a verification chamber 208.

[00116] In the illustrated embodiment, sample pump 106 is in flow communication with calibrant chamber 108 via valve 120, and is in flow communication with verification chamber 208 via a valve 220. In the illustrated embodiment, valve 120 includes a three-way valve 120, such that, based on a position of valve 220, gas 114 output from sample pump 106 will flow through valve 120 to calibrant chamber 106, will flow through valve 120 to valve 220, or will not flow through valve 120. Moreover, valve 220 includes a three-way valve 220, such that, based on a position of valve 220, gas 114 output from sample pump 106 through valve 220 will flow through valve 220 to verification chamber 208, will flow through valve 220 to an exhaust outlet, or will not flow through valve 220.

[00117] When detection system 100 is operating to verify a previous calibration of detector 102, valve 220 is positioned such that carrier gas 116 from sample pump 106 flows through valve 120, through valve 220, and through verification chamber 108. Carrier gas 116 flows through verification chamber 208 to "pick up" the verification substance therein and actively deliver the verification substance to detector 102. The combination of carrier gas 116 and the verification substance carried therein is referred to herein as "verification gas" 224. Verification chamber 208 is in flow communication with detector 102 via a valve 222. In the illustrated embodiment, valve 222 includes a two-way valve 222, such that, based on a position of valve 222, verification gas 224 will flow through valve 222 to detector 102 or will not flow through valve 222. To verify a previous calibration of detector 102, valve 222 is positioned such that verification gas 224 flows through valve 222 and to detector 102 for detection of the verification substance. Having internal verification chamber 208 enables detection system 100 to use less verification substance than alternative detection systems employing external traps, as detection system 100 is designed to channel the verification gas 224 directly to detector 102. Additionally or alternatively, detection system 100 channels the calibrant gas 124 through desorber 104 to detector 102.

[00118] In the exemplary embodiment, the verification substance includes at least one of Dibenzylamine, Diethylphenylmalonate, Diisopropylphenol, 2,4-Dimethylpyridine, Dioctylphthalate, Dinitrotoluene, Dipropylene glycol monomethylether, 2,6-Di-t-butylpyridine, Ethyl salicylate, Hexachloroethane, Hexaphenylbenzene, 4-Methyl-2,6-di-tert-butyl phenol, Methyl salicylate, Nicotinamide, 4-Nitrobenzonitrile, 5-Nitrovanillin, Pentachloroethane, Trihexylamine, and combinations thereof. In some embodiments, the calibrant and the verification substance include the same substance. In other embodiments, the calibrant and the verification substance include at least one different substance. Whatever particular substance the verification substance is, the verification substance is "known" to detector 102 (e.g., to an operator thereof) prior to verification of the calibration of detector 102 using the verification substance. Detector 102 generates a detected signal of the verification substance, adjusts the detected signal by the calibration unit, and compares the adjusted signal to a "library" of expected signals representative of different substances, including the verification substance. If the adjusted signal is within a particular range of the expected signal (e.g., with one standard deviation), the calibration of detector 102 is considered to be verified. If the calibration is not verified, in some embodiments, an operator of detection system 100 repeats the calibration process described above.

[00119] In some embodiments, verification chamber 208 includes from about 10 mg to about 500 g of the verification substance. In some embodiments, verification chamber 208 includes from about 50 mg to about 250 g of the verification substance, from about 25 mg to about 100 g of the verification substance, or from about 20 mg to about 50 g of the verification substance. More specifically, in some embodiments, verification chamber 208 includes from about 0.1 g to about 10 g of the verification substance.

[00120] In some embodiments, verification chamber 208 includes a permeation tube (not specifically shown) including the verification substance. The permeation tube is configured to emit the verification substance at a particular, predetermined rate. The verification substance is emitted from the permeation tube at an emission rate of from about 0.4 μg/min to about 30 μg/min. In some embodiments, the verification substance is emitted from the permeation tube at an emission rate of from about 0.5 μg/min to about 25 μg/min, from about 0.75 μg/min to about 20 μg/min, or from about 0.9 μg/min to about 10 μg/min. In some embodiments, the verification substance is emitted from the permeation tube at an emission rate of from about 1 μg/min to about 2 μg/min. In other embodiments, verification chamber 208 includes a control valve (not specifically shown) configured to release the verification substance at a particular, predetermined rate. The control valve releases the verification substance at an emission rate of from about 0.4 μg/min to about 30 μg/min. In some embodiments, the control valve releases the verification substance at an emission rate of from about 0.5 μg/min to about 25 μg/min, from about 0.75 μg/min to about 20 μg/min, or from about 0.9 μg/min to about 10 μg/min. In some embodiments, the control valve releases the verification substance at an emission rate of from about 1 μg/min to about 2 μg/min. Carrier gas 116 flows into verification chamber 208 and "picks up" the verification substance released from the permeation tube and/or by the control valve for analysis by detector 102 and verification of the calibration of the detector 102.

[00121] In some embodiments, verification of the calibration of detector 102 includes ionization of the verification substance in verification gas 224. Detector 102 detects and records at least one mobility spectrum (and/or differential mobility or mass spectrum) of the ionized verification substance. In the exemplary embodiment, the mobility spectrum is plotted on axes corresponding to drift time and count. The mobility spectrum is automatically shifted on the drift time axis (and/or a compensation voltage axis or mass axis, according to a type of detector 102) according the calibration unit(s), determined above. The mobility spectrum includes a peak, which, once the mobility spectrum is adjusted, should correspond to the detected verification substance. Detector 102 identifies the drift time associated with the adjusted peak. In some embodiments, detector 102 accesses the library of known mobility spectra. Detector 102 identifies the known spectrum for the verification substance and determines whether the adjusted peak is within a particular range of the known peak for the verification substance. Detector 102 further records the adjusted and verified mobility spectrum and drift time. If the adjusted peak is within a defined range of the known peak, the calibration of detector 102 is verified. If the calibration is not verified, in some embodiments, an operator of detection system 100 repeats the calibration process described above.

[00122] In some embodiments, the verification of the calibration of detector 102 is performed at fixed intervals. Moreover, in some embodiments, the fixed intervals are predetermined, for example, by an operator (not shown) of detection system 100 and/or by preprogramming detection system 100. In some embodiments, the fixed interval is between about 10 minutes and about 24 hours, between about 15 minutes and about 8 hours, between about 30 minutes and about 4 hours, between about 1 hour and about 3 hours, about 30 minutes, or about 2 hours. In some embodiments, the fixed interval is "flexible," in that additional verifications is performed before the fixed interval has fully elapsed. For example, in some embodiments, detection system 100 is pre-programmed to shorten one or more intervals if the verification process indicates low confidence in the calibration. Additionally or alternatively, the calibration of detector 102 is verified "on-demand," at a time determined by a user (e.g., an operator) of detection system 100. Additionally or alternatively, the calibration of detector 102 is verified substantially continuously (e.g., without substantial pause) after each calibration of detector 102. In some embodiments, the calibration of detector 102 is verified within a predetermined amount of time after the calibration. The predetermined amount of time, in various embodiments, is between about 10 seconds to about 1 day after calibration, between about 15 seconds to about 12 hours after calibration, between about 30 seconds to about 2 hours after calibration, between about 45 seconds to about 10 minutes after calibration, between about 1 minute to about 5 minutes after calibration, or between about 1 minute and about 2 minutes after calibration. Additionally or alternatively, the calibration of detector 102 is verified on a sample-by-sample basis. [00123] In some embodiments, a temperature of verification chamber 208 is maintained before, during, and/or after verification of the calibration of detector 102. More particularly, in certain embodiments, the temperature of verification chamber 208 is maintained between about 0 °C and about 200 °C, between about 10 °C and about 150 °C, between about 15 °C and about 100 °C, or between about 20 °C and about 50°C. The temperature is maintained within verification chamber 208 by at least one of heating, cooling, and combinations thereof. For example, in some embodiments, detection system 100 includes one or more heating elements and/or cooling elements (not shown), such as heaters, fans, heating/cooling blocks, and/or any other heating/cooling elements. Additionally or alternatively, the temperature of verification chamber 208 is maintained passively, for example, at an ambient temperature of an environment around detection system 100.

[00124] FIG. 3 is a schematic diagram of another exemplary embodiment of detection system 100, as shown in FIGS. 1 and 2. More particularly, detection system 100 is shown to further include a sensitivity chamber 308, as well as a housing 300. In the illustrated embodiment, housing 300 contains all components of detection system 100, including detector 102, calibrant chamber 108, verification chamber 208, and sensitivity chamber 308.

[00125] In the illustrated embodiment, sample pump 106 is in flow communication with calibrant chamber 108 via valve 120, is in flow communication with verification chamber 208 via valve 220, and is in flow communication with sensitivity chamber 308 via a valve 320. In the illustrated embodiment, valve 120 includes a three-way valve 120, such that, based on a position of valve 120, gas 114 output from sample pump 106 will flow through valve 120 to calibrant chamber 108, will flow through valve 120 to valve 220, or will not flow through valve 120. Moreover, valve 220 includes a three-way valve 220, such that, based on a position of valve 220, gas 114 output from sample pump 106 through valve 220 will flow through valve 220 to verification chamber 208, will flow through valve 220 to valve 320, or will not flow through valve 220. In addition, valve 320 includes a three-way valve 320 such that, based on a position of valve 320, gas 114 output from sample pump 106 will flow through valve 320 to sensitivity chamber 308, will flow through valve 320 to detector 102, or will not flow through valve 320. [00126] When detection system 100 is operating to check a sensitivity of detector 102, valves 120, 220, and 320 are positioned such that carrier gas 116 from sample pump 106 flows through valve 120, through valve 220, through valve 320 and through sensitivity chamber 308. Carrier gas 116 flows through sensitivity chamber 308 to "pick up" the sensitivity substance therein and actively deliver the sensitivity substance to detector 102. The combination of carrier gas 116 and the sensitivity substance carried therein is referred to herein as "sensitivity gas" 324. Sensitivity chamber 308 is in flow communication with detector 102 via a valve 322. In the illustrated embodiment, valve 322 includes a two-way valve 322, such that, based on a position of valve 322, sensitivity gas 324 will flow through valve 322 to detector 102 or will not flow through valve 322. To check the sensitivity of detector 102, valve 322 is positioned such that sensitivity gas 324 flows through valve 322 and to detector 102 for detection of the sensitivity substance. Having internal sensitivity chamber 308 enables detection system 100 to use less sensitivity substance than alternative detection systems employing external traps, as detection system 100 is designed to channel the sensitivity gas 324 directly to detector 102. Additionally or alternatively, detection system 100 channels the calibrant gas 124 through desorber 104 to detector 102.

[00127] In the exemplary embodiment, the sensitivity substance includes at least one of Dibenzylamine, Diethylphenylmalonate, Di-isopropylphenol, 2,4-Dimethylpyridine, Dioctylphthalate, Dinitrotoluene, Dipropylene glycol monomethylether, 2,6-Di-t-butylpyridine, Ethyl salicylate, Hexachloroethane, Hexaphenylbenzene, 4-Methyl-2,6-di-tert-butyl phenol, Methyl salicylate, Nicotinamide, 4-Nitrobenzonitrile, 5-Nitrovanillin, Pentachloroethane, Trihexylamine, and combinations thereof. In some embodiments, the calibrant and the sensitivity substance include the same substance. In other embodiments, the calibrant and the sensitivity substance include at least one different substance. In some embodiments, the verification substance and the sensitivity substance include the same substance. In other embodiments, the verification substance and the sensitivity substance include at least one different substance. Whatever particular substance the sensitivity substance is, the sensitivity substance is "known" to detector 102 (e.g., to an operator thereof) prior to checking the sensitivity of detector 102 using the sensitivity substance. Moreover, the amount of the sensitivity substance introduced into detector 102 is typically known. Detector 102 generates a detected signal of the sensitivity substance. If the signal has an appropriate magnitude according to the known amount of the sensitivity substance introduced into detector 102, the sensitivity of detector 102 is considered to be confirmed. In some embodiments, if the sensitivity is not confirmed (e.g., the sensitivity substance is not detected), an operator of detection system 100 requests service for detection system 100.

[00128] In some embodiments, sensitivity chamber 308 includes from about 10 mg to about 500 g of the sensitivity substance. In some embodiments, sensitivity chamber 308 includes from about 50 mg to about 250 g of the sensitivity substance, from about 25 mg to about 100 g of the sensitivity substance, or from about 20 mg to about 50 g of the sensitivity substance. More specifically, in some embodiments, sensitivity chamber 308 includes from about 0.1 g to about 10 g of the sensitivity substance.

[00129] In some embodiments, sensitivity chamber 308 includes a permeation tube (not specifically shown) including the sensitivity substance. The permeation tube is configured to emit the sensitivity substance at a particular, predetermined rate. The sensitivity substance is emitted from the permeation tube at an emission rate of from about 0.4 μg/min to about 30 μg/min. In some embodiments, the sensitivity substance is emitted from the permeation tube at an emission rate of from about 0.5 μg/min to about 25 μg/min, from about 0.75 μg/min to about 20 μg/min, or from about 0.9 μg/min to about 10 μg/min. In some embodiments, the sensitivity substance is emitted from the permeation tube at an emission rate of from about 1 μg/min to about 2 μg/min.

[00130] In other embodiments, sensitivity chamber 308 includes a control valve (not specifically shown) configured to release the sensitivity substance at a particular, predetermined rate. The control valve releases the sensitivity substance at an emission rate of from about 0.4 μg/min to about 30 μg/min. In some embodiments, the control valve releases the sensitivity substance at an emission rate of from about 0.5 μg/min to about 25 μg/min, from about 0.75 μg/min to about 20 μg/min, or from about 0.9 μg/min to about 10 μg/min. In some embodiments, the control valve releases the sensitivity substance at an emission rate of from about 1 μg/min to about 2 μg/min. Carrier gas 116 flows into sensitivity chamber 308 and "picks up" the sensitivity substance released from the permeation tube and/or by the control valve for analysis by detector 102 and for checking the sensitivity of the detector 102. In the exemplary embodiment, whatever the flow rate of the sensitivity substance into the sensitivity chamber 308, the amount or quantity of the sensitivity substance carried in carrier gas (and/or a concentration of the sensitivity substance in the carrier gas) is "known," for example, by the operator, such that it is determined whether the detector 102 is sensitive enough (e.g., to comply with business/client and/or legal requirements).

[00131] In some embodiments, checking the sensitivity of detector 102 includes ionization of the sensitivity substance in sensitivity gas 324. Detector 102 detects and records at least one mobility spectrum (and/or differential mobility or mass spectrum) of the ionized sensitivity substance. In the exemplary embodiment, the mobility spectrum is plotted on axes corresponding to drift time and count. The mobility spectrum is shifted on the drift time axis (and/or a compensation voltage axis or mass axis, according to a type of detector 102) according the calibration unit(s), determined above. The mobility spectrum includes a peak, which, once the mobility spectrum is adjusted, should correspond to the detected sensitivity substance. Detector 102 identifies the drift time associated with the adjusted peak. In some embodiments, detector 102 accesses the library of known mobility spectra. Detector 102 identifies the known spectrum for the sensitivity substance and determines whether the peak of the mobility spectrum has an appropriate intensity from the sensitivity substance based upon the known amount of the sensitivity substance channeled to detector 102 in sensitivity gas 324. In some embodiments, detector 102 analyzes the peak quantitatively, for example, whether the intensity of the peak is within a particular range. In some embodiments, detector 102 analyzes the peak proportionally, for example, whether the peak of the sensitivity substance is proportional to the peak of a substance of carrier gas 116, in accordance with the known amount/proportion of sensitivity substance within sensitivity gas 324. In some embodiments, detector 102 analyzes the peak qualitatively, for example, whether the intensity of the peak is above a "detection" threshold, indicating that the sensitivity substance was successfully detected. If the peak of the mobility spectrum is quantitatively, proportionally, qualitatively, and/or otherwise determined to be appropriate, the sensitivity of detector 102 is confirmed or verified. In some embodiments, detector 102 compares an intensity of the peak and/or any other characteristic of the mobility spectrum to a number of preselected detection criteria, which initiates an alarm state that is evaluated by detector 102 (e.g., using one or more software applications). Rather than imposing a binary response to one threshold, detector 102 is programmed to evaluate an alarm strength of the alarm state, wherein a low alarm strength represents a lower level of detection or lower confidence in a detection. A higher alarm strength represents a higher level of detection or higher confidence in a detection.

[00132] In some embodiments, the sensitivity check of detector 102 is performed at fixed intervals. Moreover, in some embodiments, the fixed intervals are pre-determined, for example, by an operator (not shown) of detection system 100 and/or by pre-programming detection system 100. In some embodiments, the fixed interval is between about 10 minutes and about 24 hours, between about 15 minutes and about 8 hours, between about 30 minutes and about 4 hours, between about 1 hour and about 3 hours, about 30 minutes, or about 2 hours. In some embodiments, the fixed interval is "flexible," in that additional sensitivity checks are performed before the fixed interval has fully elapsed. For example, in some embodiments, detection system 100 is pre-programmed to shorten one or more intervals if the sensitivity check indicates low confidence in detector 102 sensitivity. Additionally or alternatively, the sensitivity of detector 102 is checked "on-demand," at a time determined by a user (e.g., an operator) of detection system 100. Additionally or alternatively, the sensitivity of detector 102 is checked after each calibration of detector 102. In some embodiments, the sensitivity of detector 102 is checked substantially continuously (e.g., without substantial pause) after each calibration and/or verification of calibration of detector 102. In some embodiments, the sensitivity of detector 102 is checked within a predetermined amount of time after the calibration and/or the verification of the calibration. The predetermined amount of time, in various embodiments, is between about 10 seconds to about 1 day after calibration and/or verification, between about 15 seconds to about 12 hours after calibration and/or verification, between about 30 seconds to about 2 hours after calibration and/or verification, between about 45 seconds to about 10 minutes after calibration and/or verification, between about 1 minute to about 5 minutes after calibration and/or verification, or between about 1 minute and about 2 minutes after calibration and/or verification. Additionally or alternatively, the sensitivity of detector 102 is checked on a sample- by-sample basis.

[00133] In some embodiments, a temperature of sensitivity chamber 308 is maintained before, during, and/or after checking the sensitivity of detector 102. More particularly, in certain embodiments, the temperature of sensitivity chamber 308 is maintained between about 0 °C and about 200 °C, between about 10 °C and about 150 °C, between about 15 °C and about 100 °C, or between about 20 °C and about 50 °C. The temperature is maintained within sensitivity chamber 308 by at least one of heating, cooling, and combinations thereof. For example, in some embodiments, detection system 100 includes one or more heating elements and/or cooling elements (not shown), such as heaters, fans, heating/cooling blocks, and/or any other heating/cooling elements. Additionally or alternatively, the temperature of sensitivity chamber 208 is maintained passively, for example, at an ambient temperature of an environment around detection system 100.

[00134] In some embodiments, the verification substance, calibrant, and sensitivity substance are preferably but not necessarily separate and distinct substances that are located in different physical chambers of the detection system. The different chambers typically have at least one or more walls, barriers, membranes, or other members, physically separating each chamber from each other, thereby resulting in a physical separation of the verification substance, calibrant, and sensitivity substance from each other, even though the different chambers may be in direct or indirect flow communication with each other. Therefore, it should be appreciated by one of ordinary skill in the art that 1) the verification substance chamber 208 and the calibrant chamber 108 are typically separate chambers having at least one or more walls, barriers, membranes, or other members physically separating the verification substance chamber 208 from the calibrant chamber 108, thereby resulting in the physical separation of the calibrant and the verification substance, 2) the verification substance chamber 208 and the sensitivity substance chamber 308 are typically separate chambers having at least one or more walls, barriers, membranes or other members physically separating the verification substance chamber 208 from the sensitivity substance chamber 308, thereby resulting in the physical separation of the sensitivity substance and the verification substance, 3) the calibrant chamber 108 and the sensitivity substance chamber 308 are typically separate chambers having at least one or more walls, barriers, membranes or other members physically separating the calibrant chamber 108 from the sensitivity substance chamber 308, thereby resulting in the physical separation of the calibrant and the sensitivity substance, and/or 4) the calibrant chamber 108, verification substance chamber 208 and the sensitivity substance chamber 308 are typically each separate chambers having at least one or more walls, barriers, membranes or other members physically separating each of the calibrant chamber 108, verification substance chamber 208, and sensitivity substance chamber 308 from each other, thereby resulting in the physical separation of the calibrant, sensitivity substance, and the verification substance.

[00135] It should be understood that other embodiments of detection system 100 includes different combinations of the calibration chamber 108, verification chamber 208, and sensitivity chamber 308 than are illustrated herein. Moreover, in some embodiments, detection system 100 includes at least one of calibration chamber 108, verification chamber 208, and/or sensitivity chamber 308. It should also be understood that detection system 100 includes additional and/or alternative components in some embodiments. For example, in some embodiments, detection system 100 includes a sample pre-separation component (e.g., a chromatographic pre-separator) to separate a vaporized sample before the sample is channeled into detector 102.

[00136] Moreover, in some embodiments, detection system 100 includes one or more additional or alternative components such that one or more of the calibration, calibration verification, and sensitivity check are performed using a method other than the internal method described above. For instance, in one example embodiment, detection system 100 includes a calibration component (not shown) configured to perform calibration of detector 102 other than internally, such as an external calibrant trap. The external calibrant trap includes a calibrant that is introduced to detector 102 through desorber 104. In some embodiments, verification and sensitivity checking of detector 102 is subsequently performed internally, as described herein.

EXAMPLES

[00137] The following examples describe or illustrate various embodiments of the present disclosure. Other embodiments within the scope of the appended claims will be apparent to a skilled artisan considering the specification or practice of the disclosure as described herein. It is intended that the specification, together with the Examples, be considered exemplary only, with the scope and spirit of the disclosure being indicated by the claims, which follow the Examples.

Example 1 - Calibration [00138] Example 1 is an exemplary embodiment of calibration of detector 102 (shown in FIGS. 1-3) using the systems and methods described herein. In particular, FIG. 4 is an exemplary embodiment of a screen-capture 400 of a calibration process performed by detection system 100 (also shown in FIGS. 1-3), and FIG. 5 is another exemplary embodiment of a screen- capture 500 of a confirmation of another calibration process performed by the detection system 100. In this example, a calibrant gas (e.g., calibrant gas 124) including a carrier gas (e.g., carrier gas 116, both shown in FIGS. 1-3) and a calibrant comprising methyl salicylate was input to detector 102 for detection of the calibrant. Screen-capture 400 and/or 500 represent content displayed, for example, on interface 136 (shown in FIG. 1).

[00139] Screen-capture 400 included a plot 402 of two mobility spectra 404, 406. In the illustrated embodiment, the first mobility spectrum 404 is a mobility spectrum of detected negative ions, and the second mobility spectrum 406 is a mobility spectrum of detected positive ions. The x-axis is the flight time of the ions in the drift tube (a "drift time") and as such represents a signature or identity of the substance detected, and the y-axis represents a number of "counts" of the substance detected. Increasing height with respect to the y-axis represents a stronger signal, indicating a higher presence of a substance. Each mobility spectrum 404, 406 included at least one peak. In this example, first mobility spectrum 404 included a first peak 408 representing detection of negative ions of the calibrant. Second mobility spectrum 406 included a second peak 410 representing detection of positive ions of the calibrant.

[00140] First peak 408 is located along the x-axis at a first drift time, and second peak 410 is located along the x-axis at a second drift time. Detector 102 accessed a library of known mobility spectra for the calibrant and determined the known negative-ion and positive-ion drift times for calibrant-substance peaks.

[00141] Similarly, screen-capture 500 included a plot 502 of two mobility spectra 504, 506 of the detected negative ions and the detected positive ions, respectively. In this example, first mobility spectrum 504 included a first peak 508 representing detection of negative ions of the calibrant. Second mobility spectrum 506 included a second peak 510 representing detection of positive ions of the calibrant. Detector 102 accessed a library of known mobility spectra for the calibrant and determined the known negative-ion and positive-ion drift times for calibrant-sub stance peaks. In the illustrated embodiment of FIG. 5, to complete the calibration process, detector 102 adjusted peaks 508, 510 such that the adjusted peaks (not shown) correspond to the correct drift times for negative and positive ions, respectively, of the calibrant, according to the library of known mobility spectra. A "calibration successful" dialog 512 is displayed, indicating that the peaks 508, 510 of the mobility spectra 504, 506 were successfully adjusted. Detector 102 recorded the amount or value of the adjustment needed for each of peaks 508, 510 and stored this value as a calibration value or calibration unit. Subsequent detected signals (e.g., when detector 102 is analyzing an unknown substance of interest) were automatically adjusted by these calibration values.

Example 2 - Verification

[00142] Example 2 is an exemplary embodiment of verification of the calibration of detector 102 using the systems and methods described herein. In particular, FIG. 6 is an exemplary embodiment of a screen-capture 600 of a verification process performed by detection system 100. In this example, a verification gas (e.g., verification gas 224, shown in FIGS. 2 and 3) including a carrier gas (e.g., carrier gas 116) and a verification substance comprising methyl salicylate were input to detector 102 for detection of the verification substance. Screen-capture 600 represented content displayed, for example, on interface 136 (shown in FIG. 1).

[00143] Screen-capture 600 included a plot 602 of two mobility spectra 604, 606. In the illustrated embodiment, the first mobility spectrum 604 is the mobility spectrum of the negative ions of the carrier gas and verification substance, and the second mobility spectrum 606 is the mobility spectrum of the positive ions of the carrier gas and verification substance. Each mobility spectrum 604, 606 included at least one peak. In this example, first mobility spectrum 604 included a first peak 608 representing detection of negative ions of the verification substance. Second mobility spectrum 606 included a second peak 610 representing detection of positive ions of the verification substance. Detector 102 had adjusted peaks 608, 610 by the calibration units determined in Example 1. In this example, detector 102 had detected peaks 608, 610 representing the verification substance, for example, by accessing the library of known substances and confirming a match of peaks 608, 610 with known peaks of a known mobility spectrum of the verification substance. In some embodiments, detector 102 accordingly displays an alert (not shown). The alert indicates successful verification of the calibration of detector 102, as the adjusted peaks 608, 610 of the verification substance were appropriately detected. If adjusted peaks 608, 610 did not correspond to known peaks of the known mobility spectrum of the verification substance, in some embodiments detector 102 does not display an alert. For instance, in some embodiments, detector 102 displays a "verification unsuccessful" dialog and/or a different alert, and/or produces a sonic alert indicating the verification was unsuccessful, such that the calibration is performed again.

[00144] FIG. 7 is a schematic diagram of an alternate embodiment of detection system 100, as shown in FIG. 1. More particularly, detection system 100 is shown to include a single internal chamber 708 that includes at least one or any combination of two or more of: a calibration substance or calibrant, a verification substance(s), a sensitivity substance(s) or a sensitivity check substance(s).

[00145] In the illustrated embodiment, sample pump 106 is in flow communication with a chamber 708 via valve 120. In accordance with an aspect of the present specification, the chamber 708 is configured to contain at least one or any combination of two or more of: a calibration substance or calibrant, a verification substance(s), a sensitivity substance(s) or a sensitivity check substance(s) (hereinafter referred together as "multiple compounds"). In some embodiments, the multiple compounds are filled in the chamber 708 as a mixture. The multiple compounds are in a liquid state, in an embodiment, and therefore it is desirable that the multiple compounds are miscible with one another. In some embodiments, the chamber 708 includes two or three permeation tubes to respectively include any two or all three of the multiple compounds (separately in the tubes) for "pick up" by the carrier gas 116 released into the chamber 708. In some embodiments, the chamber 708 includes at least one, and preferably two or three, control valves each configured to release respective constituents (that is, calibrant, verification substance and/or sensitivity substance) of the multiple compounds for "pick up" by the carrier gas 116 released into the chamber 708. In some embodiments, the chamber 708 is sub-divided into two or three sections to respectively contain any two or three constituents of the multiple compounds.

[00146] It should be appreciated that each constituent of the multiple compounds may have any level or quantity; however it is desirable that a presence of the multiple compounds does not deplete each other's ions or does not deplete any background peaks, or the peaks arising from any of the constituent compounds if otherwise introduced separately.

[00147] FIG. 8 is a flow chart illustrating a plurality of steps of a method 800 for internal calibration, verification and sensitivity checking of a detector, in accordance with an embodiment of the present specification. In an embodiment, method 800 assumes that the three operations, namely, calibration, verification and sensitivity checking, are being performed using the three respective three substances, namely, the calibrant, the verification substance and the sensitivity substance. However, in alternate embodiments of method 800, any two of the operations may be performed using any two of the respective substances for performing that operation. It should be noted that the calibrant as used for calibration, the verification substance as used for verification, and the sensitivity or sensitivity check substance as used for the sensitivity check have been described in detail with the embodiments above. The embodiment described herein differentiates the previous embodiments in that a single, internal chamber is employed to house the various substances that are used to perform the operations.

[00148] Referring now to FIG. 8 along with FIG. 7, at step 805, a mixture of a calibrant, at least one verification substance and/or at least one sensitivity substance are stored in a chamber 708. At step 810, the carrier gas 116 flows into the chamber 708 and "picks up" the mixture of substances (that is, calibrant, verification substance and/or sensitivity substance), a valve opens and vapors of the substances are introduced or released into the detector 102. In various embodiments, the mixture of substances may be released in a continuous flow, at specific intervals throughout a sampling cycle or on demand. At step 815, the detection system 100 of FIG. 7 is programmed to search for and locate a calibrant and perform necessary alignment of the drift time. Thereafter, at optional step 820, the at least one verification substance is searched and located to confirm a validity of the calibration of step 810 or of an externally performed calibration. Finally, at optional step 825, a sensitivity substance is searched and located to examine and ensure that the detector maintains an adequate level of detection sensitivity and ability against predetermined criteria.

[00149] It should be appreciated that the method 800 illustrates an order of performing the three operations depending upon whether all three operations are being performed using all three substances (that is, calibrant, verification substance and sensitivity substance) or whether any two operations are being performed using any two substances.

[00150] Exemplary embodiments of detection systems for calibrating a detector, verifying the calibration of the detector, and checking the sensitivity of the detector, and methods of operating such systems are not limited to the specific embodiments described herein, but rather, components of systems and/or steps of the methods may be utilized independently and separately from other components and/or steps described herein. For example, the methods may also be used in combination with other systems requiring calibration of a detector, verification of the calibration of the detector, and checks of the sensitivity of the detector, and are not limited to practice with only the substance detection systems and methods as described herein. Rather, the exemplary embodiment is implemented and utilized in connection with many other substance detection applications that are currently configured to determine the presence of substances of interest.

[00151] Although specific features of various embodiments of the disclosure may be shown in some drawings and not in others, this is for convenience only. In accordance with the principles of the disclosure, any feature of a drawing may be referenced and/or claimed in combination with any feature of any other drawing.

[00152] Some embodiments involve the use of one or more electronic or computing devices. Such devices typically include a processor or controller, such as a general purpose central processing unit (CPU), a graphics processing unit (GPU), a microcontroller, a reduced instruction set computer (RISC) processor, an application specific integrated circuit (ASIC), a programmable logic circuit (PLC), and/or any other circuit or processor capable of executing the functions described herein. The methods described herein may be encoded as executable instructions embodied in a computer readable medium, including, without limitation, a storage device and/or a memory device. Such instructions, when executed by a processor, cause the processor to perform at least a portion of the methods described herein. The above examples are exemplary only, and thus are not intended to limit in any way the definition and/or meaning of the term processor. [00153] This written description uses examples to disclose the disclosure, including the best mode, and also to enable any person skilled in the art to practice the disclosure, including making and using any devices or systems and performing any incorporated methods. The patentable scope of the disclosure is defined by the claims, and may include other examples that occur to those skilled in the art. Such other examples are intended to be within the scope of the claims if they have structural elements that do not differ from the literal language of the claims, or if they include equivalent structural elements with insubstantial differences from the literal language of the claims.