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Title:
TARGET BIOLOGICAL MOLECULE IDENTIFICATION METHOD, BEADS FOR TARGET BIOLOGICAL MOLECULE IDENTIFICATION USE, SET OF BEADS, AND TARGET BIOLOGICAL MOLECULE IDENTIFICATION DEVICE
Document Type and Number:
WIPO Patent Application WO/2017/204294
Kind Code:
A1
Abstract:
A target biological molecule identification method, said method comprising the steps of: (a) bringing a sample containing a biological molecule into contact with multiple beads having, immobilized thereon, a ligand which can bind to the target biological molecule and a nucleic acid for beads identification use which corresponds to the type of the ligand; (b) placing the multiple beads, which have been brought into contact with the sample, in each of reaction vessels; (c) adding a reagent necessary for a nucleic acid extension reaction to each of the reaction vessels; (d) carrying out a nucleic acid extension reaction in each of the reaction vessels; (e) measuring the amount of protons generated in each of the reaction vessels during the nucleic acid extension reaction; (f) determining the presence or absence of the extension of a nucleic acid in each of the reaction vessels on the basis of the amount of protons generated; and (g) identifying the sequence for the nucleic acid for beads identification use in each of the reaction vessels, and identifying the type of the ligand in each of the reaction vessels on the basis of the sequence for the nucleic acid for beads identification use.

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Inventors:
TANAKA SHUHEI (JP)
HAMADA RYO (JP)
Application Number:
PCT/JP2017/019530
Publication Date:
November 30, 2017
Filing Date:
May 25, 2017
Export Citation:
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Assignee:
NIPPON KOGAKU KK (JP)
International Classes:
C12M1/00; C12Q1/68; G01N33/68
Domestic Patent References:
WO2015172080A12015-11-12
WO2015166768A12015-11-05
WO2014124338A12014-08-14
WO2015168161A22015-11-05
WO2014176575A12014-10-30
WO2008107014A12008-09-12
Foreign References:
JP2010193885A2010-09-09
US5763176A1998-06-09
JP2015092163A2015-05-14
JP2009195160A2009-09-03
US20130190191A12013-07-25
JP2005245223A2005-09-15
Other References:
MARGULIES, MARCEL ET AL.: "Genome sequencing in microfabricated high-density picolitre reactors", NATURE, vol. 437, 15 September 2005 (2005-09-15), pages 376 - 380, XP002398505
YANG, LIU ET AL.: "Single- Cell , Multiplexed Protein Detection of Rare Tumor Cells Based on a Beads-on-Barcode Antibody Microarray", ANALYTICAL CHEMISTRY, vol. 88, 20 September 2016 (2016-09-20), pages 11077 - 11083, XP055553535
See also references of EP 3467121A4
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
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