Title:
TARGET SEQUENCE SPECIFIC ALTERATION TECHNOLOGY USING NUCLEOTIDE TARGET RECOGNITION
Document Type and Number:
WIPO Patent Application WO/2019/039417
Kind Code:
A1
Abstract:
Provided is a method for targeting a target nucleotide sequence. The method includes introducing, into a cell: (i) CRISPR type I-D related proteins Cas5d, Cas6d, and Cas7d, or nucleic acids encoding these proteins; and (ii) a guide RNA which includes a sequence complementary to said target nucleotide sequence and a common repetitive sequence derived from CRISPR gene locus before and after said complementary sequence, or a DNA encoding said guide RNA.
Inventors:
OSAKABE KEISHI (JP)
OSAKABE YURIKO (JP)
OSAKABE YURIKO (JP)
Application Number:
PCT/JP2018/030607
Publication Date:
February 28, 2019
Filing Date:
August 20, 2018
Export Citation:
Assignee:
UNIV TOKUSHIMA (JP)
International Classes:
C12N15/09; C12N15/113; C12N15/63; C07K14/195
Domestic Patent References:
| WO2017066497A2 | 2017-04-20 | |||
| WO2015155686A2 | 2015-10-15 | |||
| WO2017043573A1 | 2017-03-16 |
Foreign References:
| JP2017512481A | 2017-05-25 | |||
| JP2015503535A | 2015-02-02 |
Other References:
MAKAROVA KS ET AL.: "SnapShot: Class 1 CRISPR-Cas Systems", CELL, vol. 168, no. 5, February 2017 (2017-02-01), pages 946 - 946, XP029932494, DOI: doi:10.1016/j.cell.2017.02.018
YANG C. ET AL.: "Comparative genomics reveals diversified CRISPR-Cas systems of globally distributed Microcystis aeruginosa, a freshwater bloom-forming cyanobacterium", FRONT. MICROBIOL., vol. 6, February 2019 (2019-02-01), pages 394, XP055579720
MORISAKA H . ET AL.: "Genome editing in mammalian cells by cascade and Cas3", J . INVEST, DERMATOL., vol. 137, no. 5, May 2017 (2017-05-01), pages 84, 490, XP055561205, DOI: doi:10.1016/j.jid.2017.02.506
See also references of EP 3674404A4
YANG C. ET AL.: "Comparative genomics reveals diversified CRISPR-Cas systems of globally distributed Microcystis aeruginosa, a freshwater bloom-forming cyanobacterium", FRONT. MICROBIOL., vol. 6, February 2019 (2019-02-01), pages 394, XP055579720
MORISAKA H . ET AL.: "Genome editing in mammalian cells by cascade and Cas3", J . INVEST, DERMATOL., vol. 137, no. 5, May 2017 (2017-05-01), pages 84, 490, XP055561205, DOI: doi:10.1016/j.jid.2017.02.506
See also references of EP 3674404A4
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
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